Table Of Content

Volume Table of Contents IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 1 ISSN 0883-8364 JANUARY 1993 CONTENTS LETTERS TO THE EDITOR A Superior Method for Single Cell Dispersal of Rat and Tumorous Human Anterior Pituitary Tissue S. L. Atkin, A. M. Landolt, R. V. Jeffreys, and M. C. White Fibroblast Inhibition of Tumor Cells May Be Mediated by TGF-61 David Kirk, Todd Broberg, Juan C. Irwin, William C. Kenney, and Lawrence W. Jones CA?* and Suspension Growth of Salmonid Embryo Cells Yasushi Wada, Hideki Nakano, and Masahide Hasobe CELLULAR MODELS Growth of Normal Human Ovarian Surface Epithelial Cells in Reduced-Serum and Serum-Free Media W. M. Elliott and N. Auersperg Cultures of Bovine Tracheal Epithelium with Differentiated Ultrastructure and lon Transport M. Kondo, W. E. Finkbeiner, and J. H. Widdicombe Organ-Specific Change in Dolichos biflorus Lectin Binding by Myocardial Endothelial Cells During In Vitro Cultivation Johanna Plendl, Laura Hartwell, and Robert Auerbach Crude Liver Membrane Fractions as Substrate Preserve Liver-Specific Functions in Long-Term, Serum-Free Rat Hepatocyte Cultures Bashar Saad, Hanspeter Schawalder, and Peter Maier Interstitial Cells from the Atrial and Ventricular Sides of the Bovine Mitral Valve Respond Differently to Denuding Endocardial Injury Wanda M. Lester, Ariff A.D amji, lbolya Gedeon, and Masaye Tanaka GROWTH DIFFERENTIATION AND SENESCENCE Differentiation of Isolated Murine Embryonic Palatal Epithelium in Culture: Exogenous Transforming Growth Factor Alpha Modulates Matrix Biosynthesis in Defined Experimental Conditions M. J. Dixon, M. J. M. Carette, B. B. Moser, and M. W. J. Ferguson Transient Induction of Cytochrome P450 1A1 mRNA by Culture Medium Component in Primary Cultures of Adult Rat Hepatocytes Thomas A. Kocarek, Erin G. Schuetz, and Philip S. Guzelian In Vitro Stimulation of Human Endothelial Cells by Derivatized Dextrans Didier Letourneur, Jacqueline Champion, Faouzi Slaoui, and Jacqueline Jozefonvicz Transforming Growth Factor-Beta: Signal Transduction Via Protein Kinase C In Cultured Embryonic Vascular Smooth Muscle Cells Robert W. Wrenn, Claire L. Raeuber, Lee E. Herman, Wendy J. Walton, and Thomas H. Rosenquist .... In Vitro Culture of Blood Cells from the Colonial Protochordate Botrylius schlosseri Baruch Rinkevich and Claudette Rabinowitz ERRATA IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 2 ISSN 0883-8364 FEBRUARY 1993 CONTENTS LETTERS TO THE EDITOR Serum-Free Medium for Long-Term Growth, Freezing, Cloning and Fusion of Myelomas and Hybridomas J-Lang Li, Ying-Jie Li, Ming-Hui Ouyang, and Yi-Bing Peng Endothelial Proliferation and Adhesivity lroka J. Udeinya Morphologic Study of Angiogenesis In Vitro Tatsuro Ishibashi, Katsuo Sueishi, Toshinori Murata, and Hajime Inomata A Serum-Free Medium for Human Epidermal-Like Cells P. Thomas lype, Gary D. Stoner, Basliel W. Gabriel, and M. Edward Kaighn Cell Cultures of Sponges Clathrina and Polymastia Michelle Klautau, Marcio R. Custodio, and Radovan Borojevic “Normal” Breast Cells Adjacent to a Tumor Grown in Long-Term Three-Dimensional Culture R. Beaupain, C. Mainguené, D. Brouty-Boyé, P. Planchon, and V. Magnien TGF-8 Gene Expression Depends on Tissue Architecture D. Theodorescu, C. Sheehan, and R. S. Kerbel New Model of Vascular Cell Repair In Vitro Valery Kolpakov, Anna Di Sciullo, Concetta Amore, Mimmo Nasuti, Licia lacoviello, Andreina Poggi, and Maria Benedetta Donati A Novel Human Glioma Cell Line from Pituitaries Wu-Nan Wen and Liming Chang Insulin-Like Growth Factor-ll Receptor Expression in Normal and N-MethyI-N’-Nitro-Nitrosoguanidine Exposed Cell Lines: Assessment by Flow Cytometry William H. Thornton, Jr., Louise Barnett, and Ruth S. MacDonald Density Gradient Separation of Growth Plate Chondrocytes Dennis E. Carey, Mauro Alini, Yasumoto Matsui, and A. Robin Poole CELLULAR AND MOLECULAR TOXICOLOGY Factors Regulating the Emergence of Spontaneous and X-Ray-induced Variants in Primary Rat Tracheal Epithelial Cell Cultures M. Terzaghi-Howe CELLULAR MODELS A Cobblestone Cell Isolated from the Human Omentum: The Mesothelial Cell; Isolation, Identification, and Growth Characteristics Apollo Pronk, Piet Leguit, Arthur A. G. M. Hoynck van Papendrecht, Edwin Hagelen, Theo J. M. V. van Vroonhoven, and Henri A. Verbrugh Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells Paul G. McGuire, Helen M. Walker-Caprioglio, Sally A. Little, and Linda J. McGuffee IMMUNOLOGY Retinoic Acid Modulation of a(1-»2) Fucosyltransferase Activity and Sensitivity of Tumor Cells to LAK-Mediated Cytotoxicity N. Labarriére, J.-P. Piau, R. Zennadi, P. Blanchardie, M. Denis, and P. Lustenberger GROWTH, DIFFERENTIATION AND SENESCENCE Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord In Vitro Marilyn J. Anderson Production of Autostimulatory Growth Factors by the Human Carcinoma Line, RPMI 2650 Breda M. Carey, Margaret Dooley, Roisin Weedle, and Martin Clynes Polarized Secretion of Tissue-Plasminogen Activator in Cultured Thyroid Cells S. Desruisseau-Gonzalvez, P. Delori, D. Gruffat, and O. Chabaud GENETICS Transient Promoter Activity in Primary Rat Mammary Epithelial Cells Evaluated Using Particle Bombardment Gene Transfer T. Anthony Thompson, Michael N. Gould, Joseph K. Burkholder, and Ning-Sun Yang IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 3 ISSN 0883-8364 MARCH 1993 PART | CONTENTS INVITED REVIEW Cellular Models for Multiple Drug Resistance in Cancer Martin Clynes LETTERS TO THE EDITOR immortalization of Human Mammary Epithelial Cells by SV40 Large T-Antigen Involves a Two Step Mechanism Brigitte A. Van Der Haegen and Jerry W. Shay Measurement of Strain in Cultured Bone and Fetal Muscle and Lung Cells J. E. Anderson, R. S. Carvalho, E. Yen, and J. E. Scott Mitogens in Bovine Pituitary for Cultured Rat Tracheal Cells David G. Thomassen Synergistic Inhibition of Human Rhabdomyosarcoma Cells by Sodium Phenylacetate and Tretinoin Grzegorz K. Gorski, Lydia E. McMorrow, and Milton H. Donaldson 3 Liposomes can Specifically Target Entrapped Melanin to Hair Follicles in Histocultured Skin Lingna Li, Valeryi K. Lishko, and Robert M. Hoffman Aphidicolin-Resistant Human Cell Lines Resistant to UV and Bleomycin Kouzin Kamino, Ken-ichiro Fukuchi, Kiyoji Tanaka, Tetsuro Miki, Jun Nakura, Yuji Takemoto, Yuichi Kumahara, and Toshio Ogihara Human Endothelial Cell Line from an Angiosarcoma Marie L. Hoover, Vaclav Vétvicka, John M. Hoffpauir, and Carlo H. Tamburro Stability of a Murine Hybridoma is Dependent on the Clonal Line and Culture Media Nicole Kessler, Suzanne Bertrand, and Michéle Aymard CELLULAR AND MOLECULAR TOXICOLOGY Extracellular Calcium Does Not Contribute to Cryopreservation-Induced Cytotoxicity Laura S. Rhoads, Anne M. Danks, John Im, Anne Warner, Robert L. Isaacson, John Baust, and Robert G. Van Buskirk Nickel-Induced Increases in Gap Junctional Communication in the Uterine Cell Line SK-UT-1 M. Sue Marty and Rita Loch-Caruso CELLULAR MODELS Absence of Trypsinogen Autoactivation and Immunolocalizatior: of Pancreatic Secretory Trypsin Inhibitor in Acinar Cells In Vitro Ariel E. Arias, Thomas Boldicke, and Molise Bendayan GROWTH, DIFFERENTIATION AND SENESCENCE Auto and Transactivation of FGF Expression: Potential Mechanism for Regulation of Myogenic Differentiation Jonathan C. Fox and Judith L. Swain Epidermal Growth Factor and Insulin-Like Growth Factor-1 Preserve Cell Viability in the Absence of Protein Synthesis Avraham Geier, Rina Hemi, Michal Haimson, and Rachel Beery Fetal Bovine Serum and Other Sera Used in Tissue Culture Increase Epithelial Permeability Kathleen H. Mortell, Alan D. Marmorstein, and Eva B. Cramer GENETICS Characterization of the DiFi Rectal Carcinoma Cell Line Derived from a Familial Adenomatous Polyposis Patient Matilde Olive, Seema Untawale, Robert J. Coffey, Michael J. Siciliano, David M. Wildrick, Herbert Fritsche, Sen Pathak, Lorraine M. Cherry, Mark Blick, Patrice Lointier, Leor D. Roubein, Bernard Levin, and Bruce M. Boman RAPID COMMUNICATIONS IN CELL BIOLOGY Culturing of Primary Hepatocytes as Entrapped Aggregates in a Packed Bed Bioreactor: A Potential Bioartificial Liver Albert P. Li, George Barker, Dale Beck, Susan Colburn, Roger Monsell, and Carol Pellegrin IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 4 ISSN 0883-8364 APRIL 1993 CONTENTS LETTERS TO THE EDITOR Resistance to HIV-1 Infection by CD4-Positive Lymphoid Cells that Vary in their Glucocorticoid Receptors and Responses Simon Kawa, Miles W. Cloyd, and E. Brad Thompson Liposome Targeting of High Molecular Weight DNA to the Hair Follicles of Histocultured Skin: A Model for Gene Therapy of the Hair Growth Processes Lingna Li, Valeryi Lishko, and Robert M. Hoffman Polyamine-Phospholipid Complex Blocks NMDA-Activated Currents In Vitro Angela T. Gyévai, A. Toth, Vera Janossy, and L. Bodécs Chinook Salmon Embryo (CHSE-sp) Cells for Fish Virus Research Hideki Nakano, Yasushi Wada, and Masahide Hasobe Modified RPMI 1640 Culture Medium George E. Moore and David B. Hood Separation of Metastatic Clone from P-388D, Murine Tumor Cells Using a Novel Sialic Acid-Specific Lectin I. Tsuboi, M. Matsukawa, and Y. Mitsui SESSION-IN-DEPTH Invertebrate Tissue Culture as a Tool to Study Insect-Transmitted Viruses: A Brief Introduction Hans Koblet Viral Evolution and Insects as a Possible Virologic Turning Table Hans Koblet Invertebrate Tissue Culture as a Tool to Study Insect Transmission of Plant Viruses Rebecca Creamer Insect-Transmitted Vertebrate Viruses: Alphatogaviruses Victor Stollar Insect-Transmitted Vertebrate Viruses: Flaviviridae George V. Ludwig and Lauren C. Iacono-Connors CELLULAR MODELS Tumorigenic, Invasive, Karyotypic, and Immunocytochemical Characteristics of Clonal Cell Lines Derived from a Spontaneous Canine Anaplastic Astrocytoma Michael E. Berens, George Bjotvedt, Donald C. Levesque, Monique D. Rief, Joan Rankin Shapiro, and Stephen W. Coons Development of Human Fetal Lung in Organ Culture Compared with In Utero Ontogeny David Cossar, Jeanne Bell, Malcolm Lang, and Robert Hume Isolation and Characterization of Microvessel Endothelial Cells from Human Mammary Adipose Tissue P. W. Hewett, J. C. Murray, E. A. Price, M. E. Watts, and M. Woodcock GROWTH, DIFFERENTIATION AND SENESCENCE Growth Modulation of Human Tumor Cells by a Growth-Inhibiting Activity Derived from Tumorigenic V79 Chinese Hamster Cells Ichiro Ichinose, Shuji Nakano, Taito Esaki, Tatsuhiko Koga, Kenji Mitsugi, Hidenori Yamada, and Yoshiyuki Niho Replication of Adult Pancreatic-Beta Cells Cultured on Bovine Corneal Endothelial Cell Extracellular Matrix G. T. Schuppin, S. Bonner-Weir, E. Montana, N. Kaiser, and G. C. Weir IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 5 ISSN 0883-8364 MAY 1993 CONTENTS LETTERS TO THE EDITOR HL-60 Derived Metamyelocyte-Rich Cell Line Keishi Hata, Kazuki Shinohara, and Masuko Kobori The Effect of Probucol on the Proliferation of Cultured Human Umbilical Vascular Endothelial Cells Takehiko Tajika, Yukihiko Ono, Kazuhiro Ono, Mamoru Miura, and Yoshinori Kyo Examination of Cell Toxicity and Dosing Regimen in an In Vitro Chromosomal Aberration Assay V. Ann Linscombe, Ken M. Bodner, B. Bhaskar Gollapudi, and Anil K. Sinha Freshly Harvested Endothelial and Mononuclear Cells: Comparison to Cells in Culture R. J. Bing, R. Dudek, and A. Conforto Size Distribution of Native Aggrecan Aggregates of Human Articular Chondrocytes in Agarose Maria Cornelissen, Marc Dewulf, Gust Verbruggen, Pascal Hellebuyck, Anne-Marie Malfait, Leo De Ridder, and Eric M. Veys Unchecked DNA Synthesis and Blocked Cell Division Induced by Methionine Deprivation in a Human Prostate Cancer Cell Line Hui Yan Guo, Robert M. Hoffman, and Hector Herrera BIOTECHNOLOGY Failure to Obtain Hybridomas Between Human Macrophages and Human Tumoral U-937 Cells is Probably Due to Parental Macrophages Alain Bohbot, Francoise Uttwiller, Ricardo Fujita, Benedicte Mousson, Michel Fabre, Francis Oberling, and Philippe Poindron CELLULAR MODELS Variation in the Electrical Properties of Cultured Human Proximal Tubule Cells John H. Todd, Mary Ann Sens, Debra J. Hazen-Martin, John E. Bylander, Brendan J. Smyth, and Donald A. Sens Serial Culturing of Human Bronchial Epithelial Cells Derived From Biopsies Petra M. de Jong, Marianne A. J. A. van Sterkenburg, Johanna A. Kempenaar, Joop H. Dijkman, and Maria Ponec Comparative Recombinant Protein Production of Eight Insect Cell Lines T. R. Davis, T: J. Wickham, K. A. McKenna, R. R. Granados, M. L. Shuler, and H. A. Wo000..........:scssssssssesesessssseseseseseees 388 Insulin-Like Growth Factor-I is a Serum Component Stimulating Growth of Human Neuroblastoma Thomas W. Burke and Stanimir Vuk-Pavlovic GROWTH, DIFFERENTIATION AND SENESCENCE Effect of Genistein, a Tyrosine-Specific Protein Kinase Inhibitor, on Cell Rounding by pH Upshifting K. H. Sit, B. H. Bay, and K. P. Wong Vitronectin Secretion by Hepatic and Non-Hepatic Human Cancer Cells Hidetaro Yasumitsu, Noriko Seo, Emi Misugi, Hatsué Morita, Kaoru Miyazaki, and Makoto Umeda Growth of Separated and Recombined Neonatal Rat Uterine Luminal Epithelium and Stroma on Extracellular Matrix: Effects of In Vivo Tamoxifen Exposure William S. Branham, Beverly D. Lyn-Cook, Annette Andrews, and Daniel M. Sheehan Melatonin Inhibits the Proliferation of Retinal Pigment Epithelial (RPE) Cells In Vitro Hing-Sing Yu, Veronica Hernandez, Mark Haywood, and Corinne G. Wong Macrophages Release Melanocyte Dendrite Extension Factor in Response to Ultra-Violet Ray Satoshi Suzuki, Takayuki Katagiri, and Takuji Takeuchi RAPID COMMUNICATIONS IN CELL BIOLOGY Cytokeratin Profile of Imnmunomagnetically Separated Epithelial Subsets of the Human Mammary Gland Shanaz Dairkee and Hans W. Heid as IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 6 ISSN 0883-8364 JUNE 1993 CONTENTS INVITED REVIEW Anabolic-Androgenic Steroids: In Cell Culture Charles D. Kochakian and Allison A. Welder LETTERS TO THE EDITOR Properties of ATT-20 Cells in Serum-Reduced Medium Matthias F. Melzig, Jorns Fickel, Brigitta S. Savoly, Ulla Vogel, and Josef Zipper Cortical and Medullary Phenotypes within a Mouse Thymic Epithelial Cell Line Elizabeth O. Cirne-Lima, Willem van Ewijk, and Wilson Savino. Induction of Myo D Expression in NIH 3T3 Cells Produces a Differentiated Myocyte Phenotype without Passing Through a Determination-Like State Samuel S. Murray Histoculture Radiometric In Vitro Hair Growth Assay Lingna Li and Robert M. Hoffman BIOTECHNOLOGY Immunomagnetic Separation as a Final Purification Step of Liver Endothelial Cells Daniel E. Gomez, Jacqueline L. Hartzler, Robert H. Corbitt, Alexander M. Nason, and Unnur P. Thorgeirsson CELLULAR AND MOLECULAR TOXICOLOGY Botulinum Toxin A Inhibits Acetylcholine Release from Cultured Neurons In Vitro Prabhati Ray CELLULAR MODELS Quantitation of Human Choriocarcinoma Spheroid Attachment to Uterine Epithelial Cell Monolayers Nancy J. John, Manuela Linke, and Hans-Werner Denker. Mucin Synthesis and Secretion by Cultured Tracheal Cells: Effects of Collagen Gel Substratum Thickness Cynthia B. Robinson and Reen Wu Myometrial Characteristics of the Syrian Hamster Uterine Smooth Muscle Cell Line, SHM R. Kirk Riemer, Yoel Sadovsky, and James M. Roberts GROWTH, DIFFERENTIATION AND SENESCENCE Rat Tracheal Epithelial Cell Differentiation In Vitro Liisa Kaartinen, Paul Nettesheim, Kenneth B. Adler, and Scott H. Randell .. Adrenergic Stimulation of Diacylglycerol Production in Genital Tract Smooth Muscle Myocytes Robert J. Austin, Laura DiGiovonni, and Mark Phillippe Neoplastic Progression of Rat Tracheal Epithelial Cells is Associated with a Reduction in the Number of Growth Factors Required for Clonal Proliferation in Culture David G. Thomassen Effect of Maternal Serum on Viability and Function of Early Human Placental Explants Jahanara Begum-Hasan, Mary Senterman, Peter Gillett, Charlotte LaPlante Branchaud, and Beverley E. Pearson Murphy Characterization of Human Plasma Growth Inhibitory Activity on Serum-Free Mouse Embryo Cells Patrick D. Varga Weisz and David W. Barnes IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 7 ISSN 0883-8364 JULY 1993 CONTENTS LETTERS TO THE EDITOR Growth of Mycoplasma Transformed tTN129 Cells Depends on IGF-I Leo T. M. Van der Ven, Louk H. P. M. Rademakers, Alexander F. Angulo, Jacques C. Giltay, Iris Wills, Gerard H. Jansen, Irma M. Prinsen, Anne G. M. Rombouts, Paul J. M. Roholl, and Willem Den Otter Expression of Prostate-Specific Antigen in Human Prostate Specimens in In Vitro Three-Dimensional Histoculture Jack Geller, Lida Sionit, Kenneth Connors, Tyler Youngkin, and Robert M. Hoffman Staurosporine Induces Multinucleation Following Chromosome Decondensation in Colcemid-Arrested Cells Katsumasa Nakamura and Shigetoshi Antoku A Better Defined Medium for Human Prostate Cancer Cells Thomas G. Pretlow, Gregory S. Ogrinc, Saeid B. Amini, Carrie M. Delmoro, Kay F. Molkentin, James K. V. Willson, and Theresa P. Pretlow CELLULAR AND MOLECULAR TOXICOLOGY A Simplified In Vitro Model of Oxidant Injury Using Vascular Endothelial Cells Lin Li and Benjamin H. S. Lau CELLULAR MODELS Establishment of a Human Anaplastic Thyroid Cancer Cell Line Secreting Granulocyte Colony-Stimulating Factor in Response to Cytokines Yoshio Oka, Tetsuro Kobayashi, Shoichi Fujita, Nariaki Matsuura, Shigeru Okamoto, Hideki Asakawa, Atsuo Murata, and Takesada Mori Culturing the Epiblast Cells of the Pig Blastocyst Neil C. Talbot, Caird E. Rexroad, Jr., Vernon G. Pursel, Anne M. Powell, and Neil D. Nel Enhanced In Vitro Hair Growth at the Air-Liquid Interface: Minoxidil Preserves the Root Sheath in Cultured Whisker Follicles D. J. Waldon, T. T. Kawabe, C. A. Baker, G. A. Johnson, and A. E. Buhl In Vitro Characterization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney Alfonso Gonzalez, Terry D. Oberley, Janice L. Schultz, Jennifer Ostrom, and Jonathan J. Li Morphogenesis of “Duct-Like” Structures in Three-Dimensional Cultures of Human Cancerous Pancreatic Duct Cells (Capan-1) Marjorie Fanjul and Etienne Hollande Role of Thrombospondin in the Adhesion of Human Endothelial Cells in Primary Culture Veronica Morandi, Francoise Fauvel-Lafeve, Chantal Legrand, and Yves J. Legrand GROWTH, DIFFERENTIATION, AND SENESCENCE Development of Primary Culture of Ovine Fetal Hepatocytes for Studies of Amino Acid Metabolism and Insulinlike Growth Factors Susan F. Townsend, Patti J. Thureen, William W. Hay, Jr., and Michael R. Narkewicz RAPID COMMUNICATIONS IN CELL BIOLOGY An Impurity in Phenol Red Opens an Ion Channel in Cultured Human Cells Martin Lubin IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 8 ISSN 0883-8364 AUGUST 1993 CONTENTS LETTERS TO THE EDITOR Different Microvascular Endothelial Cell Phenotypes Exhibit Different Class I and II Antigens under Interferon-y Katharina Spanel-Borowski and Gregor Bein Mouse Chondrocytes in Monolayer Culture Gabriella Argentin, Rosadele Cicchetti, and Benedetto Nicoletti Miller Cell Phenotype Exhibited by Senescent RSV-Transformed Chicken Neuroretinal Cells Gail M. Seigel, John T. Hansen, Eileen L. Imperato, and Mary F. D. Notter Continuous Endothelial Cells from Adult Rat Heart Marieke C. J. G. Linssen, Frans A. van Nieuwenhoven, Adriaan M. Duijvestijn, Jan F. C. Glatz, and Ger J. van der Vusse Establishment of a Metastatic Murine Cell Line Carrying the Human c-Ha-ras Takahiro Nomura, Sadaya Matano, Gensaku Okada, Haruhiko Tokuyama, Isao Hori, Shinobu Nakamura, Tadanori Kameyama, and Kazuo Ryoyama cis-Diamminedichloroplatinum Sensitivity of Murine Reticulum Sarcoma Cells in Primary Culture and after In Vitro Passages G. Balconi, G. Damia, F. Morali, M. Marzola, G. Tagliabue, E. Erba, and M. D’Incalci Primary Culture of Lymphoid, Nerve, and Ovary Cells from Penaeus stylirostris and Penaeus vannamei Elpidio Cesar Nadala, Yuanan Lu, and Philip C. Loh Clastogenesis Induced by Streptonigrin in CHO Cells Mariana I. Testoni, Martha S. Bianchi, and Néstor O. Bianchi Zinc-Enhanced Cytotoxicity in Cisplatin Sensitive and Resistant Human Ovarian Cancer Cells Daniel L. Nicholson, Robert H. Maier, and Walter J. Pories Characterization of the Unstirred Water Layer in Cultured Brain Microvessel Endothelial Cells Ka-yun Ng, George Grass, Hank Lane, and Ronald T. Borchardt Lymphoma Cell Culture under Serum-Free Conditions Yu-cheng Dai, Xin-hua Wang, Nazir Jamal, and Hans A. Messner . Genital Tract Growth and Development-Promoting Activity from Insect Cell Lines Marcia J. Loeb and Dwight E. Lynn CELLULAR AND MOLECULAR TOXICOLOGY Mycocyte Injury by Hemin V. Bhoite-Solomon, G. Kessler-Icekson, and N. Shaklai Down Regulation of CYP 1A1 by Glucocorticoids in Trout Hepatocytes In Vitro A. K. Dasmahapatra and P. C. Lee The Role of Short Chain Fatty Acid Substrates in Aerobic and Glycolytic Metabolism in Primary Cultures of Renal Proximal Tubule Cells Richard D. Griner, Michael D. Aleo, and Rick G. Schnellmann GROWTH, DIFFERENTIATION, AND SENESCENCE Effects of Tumor Necrosis Factor and Interferon-G on Proliferation and Epidermal Growth Factor Binding in Young and Senescent WI-38 Cells Felicia L. Cianciarulo, Paul D. Phillips, and Vincent J. Cristofalo An Analysis of Dorsal Root Ganglia Differentiation Using Three Tissue Culture Systems Margit Inczedy-Marcsek, Linda Hsu, and Erich Lindner Mouse Blastocyst Immunosurgery with Commercial Antiserum to Mouse Erythrocytes Y. P. Cruz, R. S. Treichel, E. Harsay, and K. D. Chi IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 9 ISSN 0883-8364 SEPTEMBER 1993 CONTENTS LETTERS TO THE EDITOR Neonatal Pig Pancreatic Islets for Transplantation Chao- Ying Kuo, Angela Myracle, George A. Burghen, and Henry G. Herrod Effect of Hemin on Growth and DNA Synthesis of HL-60 Cells Alfred Palkowski and Aleksander F. Sikorski B16 Melanoma Cells Resistant to a Novel Sialic Acid-Specific Lectin I. Tsuboi, M. Matsukawa, and N. Sato Quantifiable Cell Suspensions from Fixed, Stained Monolayers Grown in Culture Zeid Mohamedali and Nelly Auersperg CELLULAR AND MOLECULAR TOXICOLOGY Framework for Validation and Implementation of In Vitro Toxicity Tests Alan M. Goldberg, John M. Frazier, David Brusick, Michael S. Dickens, Oliver Flint, Stephen D. Gettings, Richard N. Hill, Robert L. Lipnick, Kevin J. Renskers, June A. Bradlaw, Robert A. Scala, Bellina Veronesi, Sidney Green, Neil L. Wilcox, and Rodger D. Curren CELLULAR MODELS Sterol Metabolism and Oral Epithelial Cell Growth Gretchen B. Caughman, George S. Schuster, and Thomas R. Dirksen Human Bone Marrow Stromal Cells Express an Osteoblastic Phenotype in Culture Joelle Vilamitjana-Amedee, Reine Bareille, Francois Rouais, Arnold I. Caplan, and Marie-Francoise Harmand Influence of Cell Density and Receptor Number on the Binding and Distribution of Cell Surface Epidermal Growth Factor Receptors Charles A. Kuszynski, Keith A. Miller, and Angie Rizzino GROWTH, DIFFERENTIATION, AND SENESCENCE Redifferentiation of Proliferated Rat Hepatocytes Cultured in L15 Medium Supplemented with EGF and DMSO Toshihiro Mitaka, Ken-Ichi Norioka, and Yohichi Mochizuki Pluripotent Mesenchymal Stem Cells Reside within Avian Connective Tissue Matrices H. E. Young, E. M. Ceballos, J. C. Smith, M. L. Mancini, R. P. Wright, B. L. Ragan, Ian Bushell, and P. A. Lucas Luminol and Diazoluminomelanin as Indicators of HL-60 Cell Differentiation John G. Bruno and Johnathan L. Kiel Growth Advantage (“Clonal Dominance”) of Metastatically Competent Tumor Cell Variants Expressed under Selective Two- or Three-Dimensional Tissue Culture Conditions Janusz W. Rak and Robert S. Kerbel GENETICS Characterization of a Cell Line Derived from Zebrafish (Brachydanio rerio) Embryos Wolfgang Driever and Zehava Rangini IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY — ANIMAL VOLUME 29A NUMBER 10 ISSN 1071-2690 OCTOBER 1993 CONTENTS LETTERS TO THE EDITOR Effects of Methylcellulose on Epithelial Cells Stephen Gallik, Anne Bradshaw, and Margaret van Wambeck Limit Growth of Human Fibroblasts Superinoculated on Fixed Confluent Monolayers Hideo Matsuoka, Motonosuke Furusawa, Hirotsugu Tomoda, Yousuke Seo, and Keizo Sugimachi Protein Kinase Inhibitor, Staurosporine, Prevents Okadaic Acid- or Caffeine-Induced Chromosome Condensation Katsumasa Nakamura and Shigetoshi Antoku CELLULAR AND MOLECULAR TOXICOLOGY Neoplastic Transformation and Characterizaton of Human Fibroblasts by Treatment with “Co Gamma Rays and the Human c-Ha-ras Oncogene I. Jahan, K. Mihara, L. Bai, and M. Namba Extracellular Matrix Components Affect the Pattern of Protein Synthesis of Endothelial Cells Responding to Hyperthermia Nika V. Ketis, Jack Lawler, and William G. Bendena CELLULAR MODELS Expression of Elastin, Smooth Muscle Alpha-Actin, and c-Jun as a Function of the Embryonic Lineage of Vascular Smooth Muscle Cells Preston F. Gadson, Jr., Candace Rossignol, Judy McCoy, and Thomas H. Rosenquist GROWTH, DIFFERENTIATION, AND SENESCENCE A 29 000 Molecular Weight Fraction From Fetal Rat Liver Adhering Cells That Cooperates with Erythropoietin in Stimulating the Growth of Erythroid Progenitors In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso- N-Methylurea Using a Collagen Gel Matrix Culture Jeffrey R. Laduca and Dilip K. Sinha Lysozyme as a Regulator of Interleukin-2-Activated Lymphocyte Proliferation Noritsugu Yabe, Kouji Komiya, Tetsuya Takezono, and Hisao Matsui Exogenous HSP70 Becomes Cell Associated, But Not Internalized, by Stressed Arterial Smooth Muscle Cells A. Daniel Johnson and Michael Tytell Cultured Aedes Albopictus Mosquito Cells Synthesize Hormone-Inducible Proteins Que Lan, Anna Gerenday, and Ann Marie Fallon INFECTIOUS DISEASE / CELLULAR PATHOLOGY Mycoplasma Detection by PCR Analysis Anne Hopert, Cord C. Uphoff, Manfred Wirth, Hansjérg Hauser, and Hans G. Drexler BOOK REVIEW ERRATUM