Immunological Approaches to the Diagnosis and Therapy of Breast Cancer

268 Pages·1987·10.28 MB·English

by  Arnold S. Dion

IMMUNOLOGICAL APPROACHES TO
THE DIAGNOSIS AND THERAPY
OF BREAST CANCER
IMMUNOLOGICAL APPROACHES TO
THE DIAGNOSIS AND THERAPY
OF BREAST CANCER
Edited by
Ro berto L. Ceriani
John MIIir Cancer and Aging Research Institute
W"'nut Creek, C"'ifornia
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
International Workshop on Monoclonal Antibodies and Breast Cancer (2nd: 1986: San
Francisco, Calif.)
Immunological approaches to the diagnosis and therapy of breast cancer.
"Proceedings of the Second International Workshop on Monoclonal Antibodies and Breast
Cancer, held November 20-21,1986, in San Francisco, California"-T.p. verso.
Includes bibliographies and index.
1. Breast-Cancer-Immunological aspects-Congresses. 2. Antibodies, Monoclonal
Diagnostic use-Congresses. 3. Antibodies, Monoclonal-Therapeutic use-Congresses. 4.
Breast - Cancer - Immunotherapy - Congresses. 5. Immunodiagnosis - Congresses. I.
Ceriani, Roberto L. II. Title. [DNLM: 1. Antibodies, Monoclonal-immunology-congresses.
2. Breast Neoplasms-immunology-congresses. W3 IN932662nd 1986i / WP 879161 1986i]
RC280.B8158 1986 616.99'449075 87-15373
ISBN-13: 978-1-4612-9059-9 e-ISBN-13: 978-1-4613-1903-0
DOl: 10.1007/978-1-4613-1903-0
Proceedings of the Second International Workshop on Monoclonal Antibodies and
Breast Cancer, held November 20-21, 1986, in San Francisco, California
© 1987 Plenum Press, New York
Softcover reprint of the hardcover 1s t edition 1987
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N.Y. 10013
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfliming,
recording, or otherwise, without written permission from the Publisher
2ND INTERNATIONAL WORKSHOP ON MONOCLONAL ANTIBODIES AND
BREAST CANCER
San Francisco, California
November 20-21, 1986
organized by the John Muir Cancer & Aging Research Institute,
with the cooperation of the International Association for
Breast Cancer Research.
CHAIRPERSON Dr. Roberto L. Ceriani
John Muir Cancer & Aging Research Institute
ORGANIZING COMMITTEE
Chairpersons: Dr. Roberto L. Ceriani
Dr. Jean Hager-Rich (AMC Cancer Research
Center, Denver, Colorado)
Members: Ms. Shannon Jackson Dr. Jerry A. Peterson
Dr. Bernard Larner Ms. Kelly Travers
Dr. Howard Maccabee Ms. Patricia Woycheese
PROGRAM COMMITTEE
Chairperson: Dr. Roberto L. Ceriani
Members: Dr. Robert Cardiff Dr. Ricardo Mesa-Tejada
Dr., Arthur Frankel Dr. Luciano Ozzello
Dr. Kenneth McCarty, Jr. Dr. Jeffrey Schlom
Dr. William L. McGuire
v
ACKNOWLEDGEMENTS
The Organizing Committee for the 2nd International Workshop on
Monoclonal Antibodies and Breast Cancer. together with the John
Muir Cancer & Aging Research Institute. ~ratefully acknowledge
the support of the following in making the Workshop possible:
SPONSORS
COULTER IMMUNOLOGY. HIALEAH. FLORIDA
TRITON BIOSCIENCES. INC •• ALAMEDA. CALIFORNIA
Abbott Laboratories. North Chicago. Illinois
Cambridge Research Laboratory. cambridge. Massachusetts
Cetus Corporation. Emeryville. California
Medi+Physics. Inc •• Emeryville. California
Stuart Pharmaceuticals. Wilmington. Delaware
Bio-Rad Laboratories. Richmond. California
Bristol-Myers Company. New York. New York
Eli Lilly and Company. Indianapolis. Indiana
Genelabs Incorporated. San Carlos. California
CONTRIBUTORS
The Christian Brothers Winery. FM Productions. Hyclone. Micco
Office Equipment. Polysciences. Inc .• Zymed Laboratories. Inc.
PREFACE
Once again we have gathered to discover and evaluate
advances made in our ability to understand. diagnose and
possibly treat breast cancer with the new reagents provided by
monoclonal antibody techniques.
In the last two years since our first International Workshop
on Monoclonal Antibodies and Breast Cancer there has been an
enormous surge in the number and quality of applications for
these new reagents. Solid achievements have been made in
identification and quantitation of estrogen and progesterone
receptors. in histopathological diagnostic procedures. in serum
diagnosis. and we are witnessing now the first attempts to treat
breast cancer with immunoconjugates.
Cytosolic estrogen receptors can now be quantitated with
monoclonal antibodies. and also their cellular distribution can
be directly assessed histologically. In addition. monoclonal
antibodies to progesterone receptors have been generated that
show promise in having similar uses as those to the estrogen
receptor.
In the field of diagnosis. the use of monoclonal antibodies
has permitted the development of serological approaches for
early diagnosis by identifying and measuring breast epithelial
antigens in serum. and of histological approaches for
establishing criteria for breast cancer dissemination and
prognosis.
With great expectations we are all following developments in
the area of breast cancer treatment using conjugates of
anti-breast epithelial monoclonal antibodies which are now
confirming earlier reports in affecting breast tumor control.
The recent creation of newer monoclonal antibodies and
conjugates await experimentation and clinical trials to
determine their value.
As a whole. we are at the threshold of an explosive
development in uses of these reagents and the many contributions
to this 2nd International Workshop attest to this fact. The
enthusiasm and investment of many laboratories worldwide in
these new techniques and approaches stems also no doubt from the
steady progress made in basic aspects of the field for which we
have evident proof here.
This 2nd Workshop was made possible by the generosity of our
Sponsors. the commitment of the members of the Program and
Organizing Committee. and the strong dedication of the John Muir
Cancer & Aging Research staff. A special mention
Ins~itute
should be made of the effort and care put into the preparation
of this volume by Ms. Shannon Jackson.
Evidence of the progress made is presented in this volume
and the excitement generated by these accomplishments make us
believe in the important role to be played by reagents produced
with monoclonal antibodies in breast cancer diagnosis and
treatment in the near future.
Roberto L. Ceriani
vm
CONTENTS
SESSIONS I AND II
Characterization of Cross-Related Antigens of Human
Milk Fat Globule Membrane and Breast Tumors
Detected By A New Monoclonal Antibody Panel ... 3
A.S. Dion. P.P. Major and M. Ishida
A Multiparametric Study by Monoclonal Antibodies in
Breast Cancer • . • . . . . • . . • . . . .. .. 21
M.I. Colnaghi. S. Menard, M.G. Da Dalt. R. Agresti.
G. Cattoretti. S. Andreola. G. Di Fronzo. M. Del
Vecchio. L. Verderio. N. Cascinelli and F. Rilke
Molecular and Immunological Analysis of Mucin
Molecules Produced by Normal and Malignant Human
Mammary Epithelial Cells . . • . . . . • 33
S. Gendler. J. Burchell. A.B. Griffiths and J.
Taylor-Papadimitriou
Single Cell Heterogeneity in Breast Cancer • • . . . . • • 41
J.A. Peterson
Monoclonal Antibody Studies of Drug Resistance . . . . . . 55
W.L. McGuire. S.A.W. Fuqua. L.G. Dressler
The Use of Multitumor/Tissue (Sausage) Blocks for
Immunohistologic Screening of Monoclonal
Antibodies . • • . . . . • . • . . . • . . • • • 63
H. Battifora
Advances Towards Clinical Relevance of Breast Carcinoma
Associated Antigens . . . • . . . . . . . . . . . . 69
F .A. Salinas
SESSION III
Detection and Assay of the 52K Estrogen-Regulated
Protease in Mammary Tumors • . .. .... ., 85
H. Rochefort. V. Cavailles. G. Freiss. D. Derocq.
G. Salazar. T. Maudelonde. S. Khalaf. H. Rogier.
F. Paolucci. B. Pau and M. Garcia
ix
Diversity of Antigenic Expression on Normal and
Malignant Human Mammary Cells Recognized By A
New Panel of Monoclonal Antibodies • • . . . . 95
E. Gero. Ph. Hageman. J. Hilkens. J. Sugar and
J. Hilgers
Immunochemical Evaluation of Estrogen Receptor and
Progesterone Receptor in Breast Cancer . .... 119
G.L. Greene and M.F. Press
Application of Monoclonal Antibodies Against Estrogen
and Progesterone Receptors in the Immunocytochemical
Evaluation of Breast Carcinomas . . . . . . • . 137
L. Ozzello. C.M. DeRose and D.V. Habif
Heterogeneity vs. Asynchrony of Receptor Expression
in Breast Cancer as Determined by Monoclonal
Antibodies . . . . . . . . . • . . • . . . . 145
L.B. Kinsel. J.L. Flowers. J. Konrath. G.S. Leight.
G.L. Greene and K.S. McCarty
Immunoaffinity Purification and Structural Analysis of
Human Breast Cancer Progesterone Receptors . .. 157
D.P. Edwards. P.A. Estes. D. EI-Ashry. E. Suba. and
J. Lawler-Heavner
SESSION IV
Analysis of the Humoral Immune Repertoire in Patients
with Cancer . . . . . . . . . . . . . . . 171
R.J. Cote. D.M. Morrissey. A.N. Houghton. H.F.
Oettgen and L.J. Old
An Improved Method for the Radiohalogenation of
Monoclonal Antibodies . . . . .. •.... 187
M.R. Zalutsky and A.S. Narula
Studies on a Epithelial/Epithelial Malignancy Associated
Antigen Associated with Human Adenocarcinomas .... 205
T.F. Bumol. J. Parrish. S.V. DeHerdt. M.E. Spearman.
R. Pohland. M.J. Borowitz. S.L. Briggs. A.L.
Baker. P. Marder. and L.D. Apelgren
A Serum Test for the Diagnosis and Monitoring the
Progress of Breast Cancer . . . . . . . .. '" 217
S. Stacker. N. Sacks. C. Thompson. C. Smart.
R. Burton. J. Bishop. J. Golder. X. Pei-xiang.
I.F.C. McKenzie
Experimental Therapy of Breast Cancer with Anti-breast
Epithelial Radioimmunoconjugates. ........ 229
R.L. Ceriani and E.W. Blank
Antibody Guided Diagnosis and Therapy of Patients with
Breast Cancer . . . .. ...... • 245
H.P. Kalofonos and A.A. Epenetos
Contributors 259
Index • • • • • 265
x
SESSIONS I AND II