Immunochemical Techniques: Part B

771 Pages·1981·16.299 MB·English

Contributors to Volume 73
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
IFTEKHAR ALAM (19), Cardeza Foundation Hospital, Halifax, Nova Scotia B3H 3G2,
for Hematologic Research, Jefferson Canada
Hospital, Philadelphia, Pennsylvania WILLIAM C. EBY (22, 23), Departments of
70191
Microbiology and Pathology, Loma Linda
J. ARENDS (10), Hormone Department, University, Loma Linda, California 92350
Statens Seruminstitut, DK-2300 Copen- H. HU~H FUDENaER6 (38), Department of
hagen, Denmark Basic and Clinical Immunology and Mi-
STRATIS AVRAMEAS (33), Un#~ d'Im- crobiology, Medical University of South
munocytochimie, D@artment de Biologie Carolina, Charleston, South Carolina
Moleculaire, Institut Pasteur, 75724 Paris 29403
Cedex ,51 France G. GALFRi~ (1), MRC Laboratory of Molecu-
ROBERT J. BUEHLER (17), Damon Diagnos- lar Biology, Hills Road, Cambridge CB2
tics, Damon Corp., Needham Heights, 2QH, England
Massachusetts 02194 R. H. GISLER (28), Pharmaceuticals Re-
J. E. BUTLER (34), Department of Micro- search Department, CIBA-GEIGY Lim-
biology, University of Iowa Medical ited, CH-4002 Basel, Switzerland
School, Iowa City, Iowa 52242 G. J. GLEICH (43), Allergic Diseases Re-
ALBERT CASTRO (35), Department of search Laboratory, Mayo Clinic, Roches-
Pathology, University of Miami School of ter, Minnesota 55905
Medicine, Miami, Florida 33101 JEAN-Luc GUESDON (33), Unit~ d'Im-
MIROSEAV CESKA (44), Department of Im- munocytochimie, D@artment de Biologie
munology, Sandoz Forschungsinstitut, Mol~culaire, Institut Pasteur, 75724 Paris
A-1235 Wien, Austria Cedex ,51 France
JOHN E. T. CORRIE (5), Medical Research CURTIS C. HARRIS (26), Laboratory of Ex-
Council, Immunoassay Team, Edinburgh perimental Pathology, National Cancer
EHI 2QW, United Kingdom Institute, Bethesda, Maryland 20205
HARRY DAUGHARTY (39), Diagnostic Prod- JOHN E. HERRMANN (15), Department of
ucts Evaluation, Center for Disease Con- Experimental Biology, Abbott Laborato-
trol, Atlanta, Georgia 30333 ries, North Chicago, Illinois 60064
J. R. DAWSON (42), Department of Mi- LEROY S. HERSH (40), Research and Devel-
crobiology and lmmunology, Duke Univer- opment Laboratories, Corning Glass
sity Medical Center, Durham, North Works, Corning, New York 13841
Carolina 27710 C. H. HEUSSER (28), Pharmaceuticals Re-
REHPOTSIRHC J. DEAN (30), Division of search Department, CIBA-GEIGY Lim-
Tumour Immunology, Chester Beatty Re- ited, CH-4002 Basel, Switzerland
search Institute, Belmont, Sutton, Surrey, DONALD R. HOFFMAN (45), Department of
England Pathology and Laboratory Medicine, East
S. L. DUNNETTE (43), Allergic Diseases Re- Carolina University School of Medicine,
search Laboratory, Mayo Clinic, Roches- Greenville, North Carolina 27834
ter, Minnesota 55905 IH-CHANG HSU (26), Laboratory of Experi-
S. L. F, OOOWTSA (32), Department of Medi- mental Pathology, National Cancer Insti-
cine. Dalhoasie University and Camp Hill tute, Bethesda, Maryland 20205
ix
X CONTRIBUTORS TO VOLUME 73
WILLIAM M. HUNIEr (5), Medical Re- ieine, Dalhousie University and Camp Hill
search Council, lmmunoassay Team. Hospital, Halifax, Nova Scotia B3H 3G2.
Edinburgh EHI 2QW, United Kingdom Canada
STEVEY W. KESSLEr (31), Department of KAZIMIERZ MAt.INOWSKI (29), Department
Medicine and Biochemistry. Uniformed of Ophthalmology, College of Physicians
Services UniversiO' of the Health Sci- and Sargeons, Columbia UniversiO', New
ences, Bethesda, Maryland 20014 York, New York I0032
J. L. KLEIN (42), The Johns Hopkins Oncol- WLADYSLAW MANSKI (29), Departments of
ogy Center, Johns Hopkins University Microbiology and Ophthalmology, Col-
School of Medicine, Baltimore, Maryland lege of Physicians and Surgeons, Colum-
21205 bia University, Nelv York, New York 10032
JENS KrOLL (3, 25), The Finsen Laboratory, V. MARKS (9), Department of Clinical
The Finsen lnst#ute, Strandboulevarden Biochemistry, University of Slirrey ,
49, 2100 Copenhagen O, Denmark Guildford, Surrey GU2 5XH, England
ROBERT LANGER (4), Department of Nutri- C. MILSTEIN (1), MRC Laboratory of Mo-
tion and Food Science, Massachusetts In- lecular Biology, Hills Road, Cambridge
stitute of Technology, Cambridge. Mas- CB2 2QH, England
sachusetts 02139, and Department of
CHRISTINE A. MOLINARO (22, 23), Depart-
Surgery, Children's Hospital Medical
ment of Pathology, Scripps "cinilC and Re-
Center, Boston, Massachusetts 02115
search Foundation, La Jolla, California
JOHN J. LANGONE (6), Laboratory of lm- 92037
munobiology, National Cancer Institute,
GIUSEPPE A. MOLINARO (22, 23), Depart-
National Institutes of Health, Bethesda,
ment of Molecular Immunology, Scripps
Maryland 20205
Clinic and Research Foundation, La
CArL-BERHL LAUrELL (24), Department of Jolla, California 92037
Clinical Chemistry, University of Lund,
NoBuo MONJI (35), Medical Research Divi-
Malmi) General Hospital, 214 Ol Malm6,
sion, American Cyanamid Co., Pearl
Sweden
River, New York 10965
DAVID E. LENZ (36), Pharmacology Jose Mu~oz (38), Unidad de lmmunologia
Branch, U.S. Army Biomedical Labora-
Clinica Facultad de Medicina, Univer-
tory, Aberdeen Proving Ground, Mary-
sidad de Los Andes, M6rida, Venezuela
land 21010
S. T. NErENBErG (46), Laboratory Service,
LAWRENCE LEVINE (19), Department of
Veteran's Administration Medical Ser-
Biochemistry, Brandeis University, Wal-
vice, Long Beach, California 90822
tham, Massachusetts 02254
FRANKHN Lira (17), Department gfPathol- A. R. NEUrATH (7), The New York Blood
Center, 310 East 67th Street, New York,
ogy, Medical College of Virginia, Virginia
New York 10021
Commonwealth University, Richmond,
Virginia 23298 EDWARD A. NEUWELT (37), UniversiO' of
J. G. LOEBER (18), Laboratory of Endo- Oregon Health Science Center, Portland,
crinology, National Institute of Public Oregon 97201
Health, 3720 BA Bilthoven, The Nether- M. J. O'SULLIVAN (9), Blond Mclndoe Insti-
lands tute for Transplantation Biology, Queen
E. JOHN MCKAY (24), Department of Clini- Victoria Hospital, East Grinstead, Sussex
cal Chemisto', University of Lund, Maim6 RHI9 3DZ, England
General Hospital, 214 lO Maim6, Sweden
DEBKUMAR PAIN (11), Department of En-
J. M. MAcSWEEN (32), Department of Med- zyme Engineering, Indian Institute of Ex-
CONTRIBUTORS TO VOLUME 37 xi
perimental Medicine, Calcutta 007 032, JUDITH L. VAITUKAITIS (2), Department of
West Bengal, India Medicine and Physiology, Boston Univer-
GEORGE H. PARSONS, JR. (14), Clinical sit), School of Medicine, Boston, Massa-
Assays, Division of Travenol Laborato- chusetts 81120
ries, Inc., 026 Memorial Drive, Cam- WILLIAM P. VANN (40), Research and De-
bridge, Massachusetts 93120 velopment Laboratories, Corning Glass
A~IRESIB POKRI~ (21), "Ruder Bo~kovi(" Works, Corning, New York 13841
Institute, Bijeni(ka ,45 P. .O Box ,6101 J. VERHOEF (18), Rudolf Magnus Institute,
4100/ Zagreb, Yagoslavia State University of Utrecht, Vondellaan ,6
R. PRASAD (46), University of Illinois Medi- 1253 GD Utrecht, ehT Netherlands
cal Center, Chicago, ll6nois 21606 GABRIEL VIRELLA (38), Department of
ZVONIMIR PU~AR (21), "Ruder Bogkovi(" Basic and Clinical Immunology and -iM
Institute, Bijeni(ka ,45 P. O. Box ,6101 crobiology, Medical University of South
10014 Zagreb, Yugoslavia Carolina, Charleston, South Carolina
3O492
SEWlOUR I. SCHLAGER (12), Department of
SHINICHIRO WATANABE (41), College of
Microbiology, University of Notre Dame,
Biomedical Technology, Osaka Univer-
Notre Dame, Indiana 65564
,'5tis Toyonaka, Osaka 560, Japan
PHILIP S. SHEPHERD (30), Department of
ELMAR W. WEILER (27), Ruhr-Universitdt
Chemical Pathology, Guy's Hospital
Bochum, Lehrstuhl fi~'r Pflanzen-
Medical School, London SEI 9RT, En-
physiologie, D 4630 Bochum ,1 Federal
gland
Republic of German),
AKIRA SHIMIZU (41), The Central Labora- LEIF WIDE (13), Department of Clinical
tory for Clinical Investigation, Osaka Chemistry, University Hospital, S-750 41
Uniw~rsity Medical School, Osaka, Uppsala, Sweden
Fukushima 553, Japan
RONALD L. WILDER (8), Arthritis and
J. W. STOEKER (28), Laborato~ of Applied Rheumatism Branch, National Institute of
Immunology, Hoffmann-La Roche Lim- Arthritis, Metabolism and Digestive Dis-
ited, CH-4002 Basel, Switzerland eases, National Institutes of Health,
Bethesda, Ma~land 50202
AVADHESHA SUROLIA (11), Molecular
Physics Unit, Indian Institute of Science, SALLY A. WILHELM (40), Research and De-
Bangalore 065 012. India velopment Laboratories, Corning Glass
Works, Corning, New York 13841
BRIAN F. TACK (8), Department of Medi-
SHAO-YAO YING (16), Laboratories for
cine, Childrens Hospital Medical Center,
Neuroendocrinology, ehT Salk Institute,
and Department of Pediatrics, Harvard
La Jolla, California 83129
Medical School, Boston, Massachusetts
02II5 ROBERT H. YOLKEN (26), Department of
Pediatrics, Johns Hopkins ,,OisrevinU
JEAN-PIERRE VAERMAN (20), Unit of Ex-
Baltimore, Maryland 50212
perimental Medicine, Universitd Catho-
lique de Louvain, International Institute MEINHART H. ZENK (27), Pharmazeutisch
of Cellular and Molecular Pathology, Biologie, Universitdt Mffnchen, D 8000
0021-B Brussels, Belgium Mffnchen ,2 Federal Republic of Germany
Preface
This volume continues the coverage of general immunochemical tech-
niques presented in Volume 70, Part A. The papers illustrate the ingenuity
characteristic of workers who have adapted the antigen-antibody reaction
to develop a variety of assays which are applicable to numerous biochem-
ical and clinical problems. Sensitivity and specificity clearly are among
the major advantages of immunoassay techniques. These properties make
these methods useful for quantification and purification of antigens repre-
senting a wide variety of chemical structures and biological activities.
Markers of high specific activity labeled with radionuclides, enzymes,
fluorescent molecules, or other probes have played an important role in
the development of immunochemical methods sensitive to the femtomole
range or less.
Much has been done with antibodies produced by classic immuniza-
tion methods, and they will continue to be important reagents for the
development and application of new and refined immunochemical tech-
nique,;. However, hybridoma technology opens up new vistas for the
future. Since a monoclonal antibody is a well-defined chemical reagent
with absolute specificity and affinity for an individual determinant, the
problems inherent in dealing with a heterogeneous population of an-
tibodies are minimized. Monoclonal antibodies can be produced in large
quantifies and reproduced exactly in different laboratories, provided the
original hybridoma is available. Perhaps in the near future, a central bank
of hybridoma cell lines will provide an "off-the-shelf" source of specific
antibodies available to scientists around the world. The investigator's only
major effort would be to grow the cells and harvest the antibody product.
The field is wide open for the development of innovative methods that will
add even another dimension to analytical technology.
We are indebted to the authors whose contributions make these vol-
umes possible. Carla Langone has continued to deal competently with the
secretarial work and Dr. Adrian Gee has done an excellent job indexing
the volumes. The continued enthusiastic support of Dr. Nathan Kaplan
and Dr. Sidney Colowick is greatly appreciated.
JOHN J. LANGONE
HELEN VAN VUNAK1S
iiix
METHODS IN ENZYMOLOGY
EDITED BY
Sidney R Colowick and Nathan O. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDIC1NE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE TA SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
.V Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
VX
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney .P Colowick Nathan O. Kaplan
VOLUME VIII. Complex Carbohydrates
Edited yb ELIZABETH F. NEUFELD DNA ROTCIV GRUBSNIG
VOLUME IX. Carbohydrate Metabolism
Edited yb WILLIS A. DOOW
VOLUME X. Oxidation and Phosphorylation
Edited yb DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRS
VOLUME XII. Nucleic Acids (Parts A and B)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
VOLUME XIII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited yb KENNETH KUSTIN
EMU.tOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb HERBERT TABOR AND CELIA WHITE TABOR
EMU.tOV XVlII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DONALD B. MCCORMICK AND LEMUEL D. WRIGHT
xvii
XVln °o, METHODS IN ENZYMOLOGY
EMULOV XIX. Proteolytic Enzymes
Edited yb GERTRUDE E. NNAMLREP DNA LASZLO DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited yb KIVlE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb ANTHONY NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited yb ANTHONY NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited yb C. H. W. HIRS DNA SERGE N. TIMASHEFF
EMULOV XXVI. Enzyme Structure (Part C)
Edited yb C. H. W. HIRS DNA SERGE N. TIMASHEFF
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by VICTOR GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by SIDNEY FLEISCHER DNA LESTER REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited by SIDNEY REHCSIELF DNA LESTER REKCAP
METHODS IN ENZYMOLOGY xix
VOLUME XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by MARTHA G. DENNIS AND EDWARD A. DEYNIS
U VOt, EM XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. JAKO~Y DNA ME1R WILCHEK
VOLUME XXXV. Lipids (Part B)
Edited by JOHN M. LOWENSTEIN
VOLUME XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
VOLUME XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
VOt, EMU XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN AND BERT W. O'MALLEV
VOt.UME XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMU._IOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited yb BERT W. O'MALLEY DNA JOEL G. HARDMAN
VOLUME XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. WOOD
VOLUME XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. WOOD
VOt.UME XLIII. Antibiotics
Edited by JOHN H. HASH
ULOV EM XLIV. Immobilized Enzymes
Edited by KLAUS HCABSOM
VOLUME XLV. Proteolytic Enzymes (Part B)
Edited by LASZLO LORAND
VOLUME XLVI. Affinity Labeling
Edited by WILLIAM B. JAKOBY DNA MEIR WILCHEK
XX METHODS IN ENZYMOLOGY
VOLUME XLVII. Enzyme Structure (Part E)
Edited by C. H. W. H1RS DNA SERGE N. TIMASHEFF
VOLUME XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA SERGE N. TIMASHEFF
VOLUME XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA SERGE N. TIMASHEFF
VOLUME L. Complex Carbohydrates (Part C)
Edited by VICTOR GINSBURG
VOLUME LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by PATRICIA A. HOFFEE DNA MARY ELLEN JONES
VOLUME LII. Biomembranes (Part C: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
VOLUME LIII. Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
VOLUME LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY FLE1SCHER DNA LESTER PACKER
VOLUME LV. Biomembranes (Part F: Bioenergetics)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
VOLUME LVI. Biomembranes (Part G: Bioenergetics)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
VOLUME LVII. Bioluminescence and Chemiluminescence
Edited by MARLENE A. DELUCA
VOLUME LVIII. Cell Culture
Edited by WILLIAM B. JAKOBY DNA ARI H. PASTAN
VOLUME LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVIE MOLDAVE DNA LAWRENCE NAMSSORG
VOLUME LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by KIVIE MOLDAVE DNA LAWRENCE NAMSSORG
METHODS IN ENZYMOLOGY xxi
EMULOV 61. Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA SERGE N. TIMASHEFF
EMULOV 62. Vitamins and Coenzymes (Part D)
Edited by DONALD B. KCIMROCCM DNA LEMUEL D. WRIGHT
EMULOV 63. Enzyme Kinetics and Mechanisms (Part A: Initial Rate and
Inhibitor Methods)
Edited by DANIEL L. HCIRUP
EMULOV 64. Enzyme Kinetics and Mechanisms (Part B: Isotopic Probes
and Complex Enzyme Systems)
Edited by DANIEL L. HCIRUP
EMULOV 65. Nucleic Acids (Part )I
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV 66. Vitamins and Coenzymes (Part E)
Edited by DONALD B. KCIMROCCM DNA LEMUEL D. WRIGHT
EMULOV 67. Vitamins and Coenzymes (Part F)
Edited by DONALD B. KCIMROCCM DNA LEMUEL D. WRIGHT
EMULOV 68. Recombinant DNA
Edited by RAY Wv
EMULOV 69. Photosynthesis and Nitrogen Fixation (Part C)
Edited by ANTHONY NAS ORTEIP
EMULOV 70. Immunochemical Techniques (Part A)
Edited by HELEN NAV SIKANUV DNA JOHN J. ENOGNAL
EMULOV 71. Lipids (Part C)
Edited by JOHN M. NIETSNEWOL
EMULOV 72. Lipids (Part D)
Edited by JOHN M. NIETSNEWOL
EMULOV 73. Immunochemical Techniques (Part B)
Edited by JOHN J. ENOGNAL DNA HELEN NAV SIKANUV