Identification of Fusarium Head Blight resistance genes in the wheat QTL-Fhb2, through forward

25 Pages·2016·2.34 MB·English

Barley Chitin Elicitor Receptor
Kinase (HvCERK1) Contributes to
Resistance against Fusarium
graminearum
Shailesh Karre, Dhananjay Dhokane, Arun Kumar, & Ajjamada C. Kushalappa
Department of Plant Science
Barley
Fourth major cereal crop

Unique dietary health benefits and exceptional malting
properties makes barley an promising food crop


Molecular Characterization
2n=14
~5.3 GB genome size
24,287 genes predicted

Canada is the fifth largest
producer of barley ( )
FAO STAT, 2014
Fusarium head blight
F. graminearum
 FHB: A most devastating disease

 Fusarium graminearum: predominant causal agent

 Causes severe losses in yield

 Reduces grain quality due to accumulation of
trichothecene mycotoxin like Deoxynivalenol

Healthy Diseased
Healthy spikelets Diseased spikelets (Gilbert and Tekauz, 2000)
Mini review on
economic losses
Province/country Year Losses in Losses in References
million metric million $
tonnes
Quebec and Ontario 1990 - 200 Windels,2000
Manitoba 1990 - 300 Windels,2000
USA 1919 2.18 - Dickson,1929
China 1990’s 1.00 - Bai & Shaner, 2004
USA 1990 - 3000 Schmale, 2003
USA 2003 - 13.6 McMullen,2012
Kansas 2008 - 57 McMullen,2012
Nebraska 2008 - 13.3 McMullen,2012
Kansas 2010 - 13 McMullen,2012
Unified efforts Needed!!
Fungicide
application
FHB
Seed
PLANT
Control
Treatment RESISTANCE
Measures
Effective
Economic
Ecofriendly

Cultural and
Biological
Practices
eg. Tillage,
waste
disposal
(Bai & Shaner, 2004)
How resistance works?

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(Kushalappa et al., 2016)
Research question?

To identify genetic controls that contributes to
FHB resistance in barley resistant cultivar
CI9831 which may be further transferred to
susceptible genotypes or the non-functional
one’s in the susceptible genotypes would be
replaced using genome editing techniques.
Phenotyping of genotypes
I. Disease severity analysis II. Fungal biomass quantification
Genotypes CI9831, Zhedar2, H106-
371 were grown in green house
Spikelets of 15 spikes were
inoculated with Fg macroconidial
spore suspension
Disease severity was accessed
(Kumar et al., 2015)
Results
Disease severity analysis and fungal biomass quantification
confirmed CI9831 as FHB resistant genotype.
Metabolo-transcriptome profiling of
barley resistant cultivar CI9831
 Experimental Design: RCBD
 Barley Genotype: CI9831 (Resistant)
 Inoculations: Pathogen and Mock
 Replications: 5 for metabolomics and 3 for transcriptomics
 Experimental Unit: Spikelets
 Sample collection: 48 hrs (transcriptomics) and 72 hrs
(metabolomics)

Plant production Pathogen production Inoculations Sample
Fusarium graminearum Pathogen c o l l e c t i o n
Strain- Z3639
Mock