IDENTIIFNICCAOTLIDO-NHAANRDDCYHCAIRTARCUTSERREILZAATTIIVOENPoOnFclCrOusLiDri-fRolEiaGlaU(LL.A)TREafD.GENES MEHTAPSAHIN-CEVIK OAFDTIHSESOUEFNRITTVAHETERIRSOIENDTQPOYURCIOETRFSOEEFRMNLEOTONEFRTDIPSDTHAFIOOLITRNOHSPTEOAHPGREHTRDYIAEADLGURFAEUTELEFOSIFCLLHMOEONLT UNIVERSITYOFFLORIDA Copyright2003 by MEHTAPSAHIN-CEVIK TomyhusbandBayram- ACKNOWLEDGMENTS Moore,1wcohuailrdolfikmeyloseuxpperrevsissomryycsoimnmcietreteger,atiftourdhee,rthaadnvkisc,e,ansudppaoprptr,eceinactoiuonratgoeDmre.ntG,loarnida uhnadveerrsetaacnhdeidngmtyhrgoouaglhsowuittthhoiustshteurdyguainddantcheeapnrdephaerlapt.ionofthismanuscript.Icouldnot Dr.WIaywannetlSoheerxtmeanndmfoyrgkriantdiltyudesetrovDirn.gMoanriamyGalcloom-mMictatgehec;r,anDrd.CfohrarltiheeirGuyhe,lpafnudl suggestionsandcommentsandforreviewingthisdissertation. alsowIownatnttotothtahnaknkDKr.imSuReuesMcohyeforrhaenrdhehlepralnadbsfuoprpotrhte;irthhaetlmpaadnedthsisupwpoorrtk;eaasnider.for1 providingthephosphoimagerandhybridizationbathformyexperiments.Iextendmy thanksandappreciationloDr.Morseforherhelpinanalysisofexpressiondata;andto Dr.Foltaforlettingmeusehislabfacilities.IwouldalsoliketothankmembersofDr. Cmlyinfer'isenldasbifnorGatihneeirsvhielllpeaannddsaurpopuonrtd;tahendwofrolrdsh(aerspiencgiathleliyrlloabBaesqumiap)mefonrt.theIstuhpapnokratllanodf friendMshyipdteheepyepsrtovtihdaendksinatnhdelaapsptrfeocuiratyieoanrs.gotomymother,Sultan,forherconstant ssMuuepphppmoorrctttaIanrndedcueTniacvloeantdfiartnoidmonhmailyslfsoaivmsiteelryts.hrAoyIutagelhnso,outEwzammnycthlgaiinfve,.eammnIyydapNttpehhrsaaernncikkinssa;ttieaontmdheyferinondm-lleamswsyslfboorvroetthhaeenridsr whom1thgiisvedimssyertsapteicoinalistdheadnikcsataend,dfloorvheistocomnystawnotndleovref,ulhelhpu,sbsaupnpdorBt,ayurnademrs(t,a’envdiikngt,o andencouragementsincethedaywemet. girl,BestcSelena,forbeingwithmeandbringingmethejoyandhappinessthatmade finishingupmyresearchandwritingmydissertationmoreenjoyable. supporFtindaulrliyn,gImythagnrkadutahteeTsutrukdiiesshatMitnhiesUtnriyveorfsiEtdyucoaftFiloornidfao.rtheirgenerousfinancial . . TABLESOFCONTENTS ATACBKLNEOSWOLFECDOGNMTEENNTTSS. LISTOFTABLES LISTOFHOURS ABSTRACT 3IIrDlfEoNliTalIaF(IL.C)ARTaIf.ONUSOIFNCGOSLUDBTRREAGCUTLIAVTEEcDDGNEANLEISBFRARROIMESPt menufurtimeImpressionStudy Kesu.RDNleanvvtleatrhAscneamlNByolsroittsheHrynbrBildoitsaAtniaolnysisofSuht-n >FCOLDINDUCEDPUTAllVt SIRseaoqpliuadetinoAcnmepolAfinfRailNcyaAsli.snoofeDNAEnds(RACE) - CRoevledrsIendrucreadnsPc!npBi0i5oncPDolNyAme(rnausnePCuhnaciinrjRseEanccOoudne(sRaInPACPR2). CPoIlId--C0I2ndcuDceNdAPIf-rCoImOPccnDcNirAfr< asEncodesaRAVI.I 5SUB-CKI.I.UIARLOCALIZATIONOFCOLDREGULATEDCLNLS 6SIMMARYANDCONCIL'SIONS I-1STOFREFERENCES BIOGRAPHICALSKETCH. .125 LISTOFTABLES Table Page 3-1.Sfurmommarcoyldo-fascecqluimeantceedaPnodnceixprruesscsiDoNnAanlailbyrasriiess.o.f..c.o..l.d..-.r..e.g..ulaledgenesidentified48 LISTOFFIGURES MuIhl'i,tgwihepeilsetmssheoPqm1uo-elCnoIcg0eycawlDiiNtghnAmPewIni-ttChoIfOprporteediincsteidnaGmeinnBoanalc CFEuoxlpnlrs-eelsresnvigeotdnhDosNefqAPu-Ieb-niCcnIedOoicfnDgPNsdmoAamrainuisnrescsopDfoNnPsAIe-PCtInIIOe-nCv0.i2rot I.AnalysisofpredictedaminoacidsequencesofPo 55--21..SSuebq-uceenlcluelaalrilgoncmaleinztatainodncolfoCniOnRgogfenCeOsRingoennieosnfeoprisduebr-mcaelllcuellalrs.localizationstudy.1.0918 IDENTIFINICCAOTLIoDROfe-NtqhHuAeAiNUrRneDiDmveYCenrHtsCsAiItRfTyoARroCfUthTSFelEoDRRreEiIgdLrZaAeAieTnTIoPIVfaOrEDtNoiPaclOotnoFFcurilCorffOiulsPLlhImDirei-ln/otRosloEoifapGlhtaUhye(LL.A)TREafD.GENES By MEHTAP$AHIN-CEVIK December2003 CMhaajiorr:DGelpoarritamAe.ntM:ooHroreticulturalSciences Citrusisacold-sensitiveplantandmostcommerciallyimportantvarietiesofcitrus aresusceptibletofreezes.Ontheotherhand.PoncirusIrifolialaisanintcrfertilcCitrus genesinvolvedincoldtoleranceinP.Irifoliala.cDNAlibrarieswereconstructedfrom2- hybridizationmethod.Atotalof192randomlypickedclones,136fromthecoldinduced libraryand56fromthecoldrepressedlibrary,weresequenced.Mostoftheseclones showedsequencehomologytopreviouslycharacterizedcold-inducedorenvironmental stress-regulatedgenesfromotherplantsthatweredepositedinGenBank. ewxiptrhecsoslido-naocfcl%imactDedNAansdwnaosnaicncclriemaasetded2-prtoob4e9s-.fAonladldyusriisnogfceoxlpdraecscsliiomnatdiaotna.shOonwethdethat partialcDNAs(PI-B05.PI-CI0.andPII-C02)showinghomologytopreviously