Identification and Characterization of cold-regulated genes in cold-hardy citrus relative poncirus trifoliata (L.) raf.

141 Pages·2003·2.1 MB·English

by  Sahin-Çevik, Mehtap

IDENTIIFNICCAOTLIDO-NHAANRDDCYHCAIRTARCUTSERREILZAATTIIVOENPoOnFclCrOusLiDri-fRolEiaGlaU(LL.A)TREafD.GENES
MEHTAPSAHIN-CEVIK
OAFDTIHSESOUEFNRITTVAHETERIRSOIENDTQPOYURCIOETRFSOEEFRMNLEOTONEFRTDIPSDTHAFIOOLITRNOHSPTEOAHPGREHTRDYIAEADLGURFAEUTELEFOSIFCLLHMOEONLT
UNIVERSITYOFFLORIDA
Copyright2003
by
MEHTAPSAHIN-CEVIK
TomyhusbandBayram-
ACKNOWLEDGMENTS
Moore,1wcohuailrdolfikmeyloseuxpperrevsissomryycsoimnmcietreteger,atiftourdhee,rthaadnvkisc,e,ansudppaoprptr,eceinactoiuonratgoeDmre.ntG,loarnida
uhnadveerrsetaacnhdeidngmtyhrgoouaglhsowuittthhoiustshteurdyguainddantcheeapnrdephaerlapt.ionofthismanuscript.Icouldnot
Dr.WIaywannetlSoheerxtmeanndmfoyrgkriantdiltyudesetrovDirn.gMoanriamyGalcloom-mMictatgehec;r,anDrd.CfohrarltiheeirGuyhe,lpafnudl
suggestionsandcommentsandforreviewingthisdissertation.
alsowIownatnttotothtahnaknkDKr.imSuReuesMcohyeforrhaenrdhehlepralnadbsfuoprpotrhte;irthhaetlmpaadnedthsisupwpoorrtk;eaasnider.for1
providingthephosphoimagerandhybridizationbathformyexperiments.Iextendmy
thanksandappreciationloDr.Morseforherhelpinanalysisofexpressiondata;andto
Dr.Foltaforlettingmeusehislabfacilities.IwouldalsoliketothankmembersofDr.
Cmlyinfer'isenldasbifnorGatihneeirsvhielllpeaannddsaurpopuonrtd;tahendwofrolrdsh(aerspiencgiathleliyrlloabBaesqumiap)mefonrt.theIstuhpapnokratllanodf
friendMshyipdteheepyepsrtovtihdaendksinatnhdelaapsptrfeocuiratyieoanrs.gotomymother,Sultan,forherconstant
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andencouragementsincethedaywemet.
girl,BestcSelena,forbeingwithmeandbringingmethejoyandhappinessthatmade
finishingupmyresearchandwritingmydissertationmoreenjoyable.
supporFtindaulrliyn,gImythagnrkadutahteeTsutrukdiiesshatMitnhiesUtnriyveorfsiEtdyucoaftFiloornidfao.rtheirgenerousfinancial
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TABLESOFCONTENTS
ATACBKLNEOSWOLFECDOGNMTEENNTTSS.
LISTOFTABLES
LISTOFHOURS
ABSTRACT
3IIrDlfEoNliTalIaF(IL.C)ARTaIf.ONUSOIFNCGOSLUDBTRREAGCUTLIAVTEEcDDGNEANLEISBFRARROIMESPt
menufurtimeImpressionStudy
Kesu.RDNleanvvtleatrhAscneamlNByolsroittsheHrynbrBildoitsaAtniaolnysisofSuht-n
>FCOLDINDUCEDPUTAllVt
SIRseaoqpliuadetinoAcnmepolAfinfRailNcyaAsli.snoofeDNAEnds(RACE) -
CRoevledrsIendrucreadnsPc!npBi0i5oncPDolNyAme(rnausnePCuhnaciinrjRseEanccOoudne(sRaInPACPR2).
CPoIlId--C0I2ndcuDceNdAPIf-rCoImOPccnDcNirAfr< asEncodesaRAVI.I
5SUB-CKI.I.UIARLOCALIZATIONOFCOLDREGULATEDCLNLS
6SIMMARYANDCONCIL'SIONS
I-1STOFREFERENCES
BIOGRAPHICALSKETCH. .125
LISTOFTABLES
Table Page
3-1.Sfurmommarcoyldo-fascecqluimeantceedaPnodnceixprruesscsiDoNnAanlailbyrasriiess.o.f..c.o..l.d..-.r..e.g..ulaledgenesidentified48
LISTOFFIGURES
MuIhl'i,tgwihepeilsetmssheoPqm1uo-elCnoIcg0eycawlDiiNtghnAmPewIni-ttChoIfOprporteediincsteidnaGmeinnBoanalc
CFEuoxlpnlrs-eelsresnvigeotdnhDosNefqAPu-Ieb-niCcnIedOoicfnDgPNsdmoAamrainuisnrescsopDfoNnPsAIe-PCtInIIOe-nCv0.i2rot
I.AnalysisofpredictedaminoacidsequencesofPo
55--21..SSuebq-uceenlcluelaalrilgoncmaleinztatainodncolfoCniOnRgogfenCeOsRingoennieosnfeoprisduebr-mcaelllcuellalrs.localizationstudy.1.0918
IDENTIFINICCAOTLIoDROfe-NtqhHuAeAiNUrRneDiDmveYCenrHtsCsAiItRfTyoARroCfUthTSFelEoDRRreEiIgdLrZaAeAieTnTIoPIVfaOrEDtNoiPaclOotnoFFcurilCorffOiulsPLlhImDirei-ln/otRosloEoifapGlhtaUhye(LL.A)TREafD.GENES
By
MEHTAP$AHIN-CEVIK
December2003
CMhaajiorr:DGelpoarritamAe.ntM:ooHroreticulturalSciences
Citrusisacold-sensitiveplantandmostcommerciallyimportantvarietiesofcitrus
aresusceptibletofreezes.Ontheotherhand.PoncirusIrifolialaisanintcrfertilcCitrus
genesinvolvedincoldtoleranceinP.Irifoliala.cDNAlibrarieswereconstructedfrom2-
hybridizationmethod.Atotalof192randomlypickedclones,136fromthecoldinduced
libraryand56fromthecoldrepressedlibrary,weresequenced.Mostoftheseclones
showedsequencehomologytopreviouslycharacterizedcold-inducedorenvironmental
stress-regulatedgenesfromotherplantsthatweredepositedinGenBank.
ewxiptrhecsoslido-naocfcl%imactDedNAansdwnaosnaicncclriemaasetded2-prtoob4e9s-.fAonladldyusriisnogfceoxlpdraecscsliiomnatdiaotna.shOonwethdethat
partialcDNAs(PI-B05.PI-CI0.andPII-C02)showinghomologytopreviously