AQUAPONICS AND HYDROPONICS: THE EFFECTS OF NUTRIENT SOURCE AND HYDROPONIC SUBSYSTEM DESIGN ON SWEET BASIL PRODUCTION by Ryan K. Dunwoody An Abstract of a thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in the Department of Biology and Agriculture University of Central Missouri November, 2013 i ABSTRACT by Ryan K. Dunwoody Development and practice of sustainable agriculture is one approach to help offset escalating environmental and production crises. Aquaponics is an emerging sustainable agriculture system that amalgamates aquaculture and hydroponics. This research compared sweet basil (Ocimum basilicum L. Nufar), yield within six constant flow recirculating systems. Two hydroponic subsystem designs, media filled and deep water culture, were employed. Independent nutrient sources consisted of General Hydroponics® Flora Series, or metabolic wastes of live channel catfish (Ictalurus punctatus) during respective trials. Weekly water quality and macronutrient assessments were recorded. Sweet basil fresh leaf mass (kg) (FLM), yield (leaf) (kg/0.6027 m2), total yield of vegetative (non-root) biomass (kg) (TVB), plant height (cm), and absolute growth rate (cm/day) (AGR) data was analyzed following each trial. For overall combined media filled and DWC aquaponic and hydroponic systems, FLM, yield, TVB, height, and AGR were significantly higher during the hydroponic trial (p=<0.001). Furthermore, there were significant differences in basil production between aquaponic and hydroponic media and DWC hydroponic subsystems (p<0.05). Additionally, there was no significant difference between aquaponic and hydroponic DWC hydroponic subsystems for FLM, yield, and TVB (p>0.05). Lastly, analysis revealed significant differences between water quality and macronutrient parameters, except for temperature (°C), D.O. (ppm), and PO 3- (ppm). 4 ii AQUAPONICS AND HYDROPONICS: THE EFFECTS OF NUTRIENT SOURCE AND HYDROPONIC SUBSYSTEM DESIGN ON SWEET BASIL PRODUCTION by Ryan K. Dunwoody A Thesis presented in partial fulfillment of the requirements for the degree of Master of Science in the Department of Biology and Agriculture University of Central Missouri November, 2013 iii © 2013 Ryan K. Dunwoody ALL RIGHTS RESERVED iv AQUAPONICS AND HYDROPONICS: THE EFFECTS OF NUTRIENT SOURCE AND HYDROPONIC SUBSYSTEM DESIGN ON SWEET BASIL PRODUCTION by Ryan K. Dunwoody November, 2013 APPROVED: Thesis Chair: Dr. Stefan Cairns Thesis Committee Member: Dr. Scott Lankford Thesis Committee Member: Dr. Mark Goodwin ACCEPTED: Chair, Department of Biology and Agriculture: Dr. Fanson Kidwaro UNIVERSITY OF CENTRAL MISSOURI WARRENSBURG, MISSOURI v ACKNOWLEDGMENTS First and most importantly I would like to thank my academic advisors: Dr. Stefan Cairns (principle Investigator), Dr. Scott Lankford (committee member), and Dr. Mark Goodwin (committee member) for all of their knowledge, help, and support provided to me throughout my academic career. Secondly, I would like to thank the University of Central Missouri for the SHCUR1 Level II Internal Grant that was awarded to Dr. Cairns that supported my research. Without these funds, this research would not have been possible. Furthermore, I would like to thank General Hydroponics for their generous donation of General Hydroponic® Flora Series. Additionally, I would like to thank the Missouri Department of Conservation Chesapeake Fish Hatchery for donating their quality channel catfish fingerlings, and the Clinton office for loaning their fish transport equipment. Moreover, I would like to thank GreenCircle Hydroponics for their supply of hydroponic materials for my study. Subsequently, I would like to thank my peers and UCM Biology support staff; Messrs. Daniel Metcalf, David Penning, Travis Lyon, Geoffrey Vincent, Alex Prentice, John Platt, Jordan Smith, John Lisek, and Ms. Glenda Carmack for all of the help and contributions with the research. In addition, I would like to give a special thanks to my research assistant Mr. Jared Kelly for his hard work and devotion to this study. Finally, I would like to thank my family and friends for all of their love and support. vi TABLE OF CONTENTS Page LIST OF TABLES ............................................................................................................. ix LIST OF FIGURES .............................................................................................................x LIST OF PLATES ........................................................................................................... xiii CHAPTER 1: INTRODUCTION ........................................................................................1 CHAPTER 2: MATERIALS AND METHODS ...............................................................22 CHAPTER 3: RESULTS ...................................................................................................32 CHAPTER 4: DISCUSSION .............................................................................................46 CHAPTER 5: CONCLUSION ..........................................................................................71 CHAPTER 6: LITERATURE CITED ...............................................................................74 APPENDICIES A. Aquaponic Sweet Basil Growth .............................................................................81 B. Hydroponic Sweet Basil Growth ...........................................................................82 C. General Hydroponics® Flora Series Expert Recirculating Feeding Schedule ......83 D. General Hydroponics® Flora Series Feeding Schedule Utilized During Hydroponic Trial ...................................................................................................84 E. Aquaponic Trial Data .............................................................................................85 F. Aquaponic and Hydroponic Foot Candle Data ......................................................99 G. Hydroponic Trial Data .........................................................................................100 vii Page H. Note : Aquaponics and Tilapia ............................................................................112 I. IACUC Approval .................................................................................................114 viii LIST OF TABLES Table Page 1.1 Mean water quality parameters sampled five times (15-Jan, 23-Jan, 31-Jan, 8-Feb, and 16-Feb) during the aquaponic trial .............................................................39 1.2 Mean water quality parameters sampled five times (17-Apr, 25-Apr, 3-May, 11-May, and 19-May) during the hydroponic trial ......................................................45 ix LIST OF FIGURES Figure Page 2.1 Aerial view of system design and orientation, as well as plant schematics .................24 2.2 Lateral view of one system design ...............................................................................26 2.3 Aerial view of one system design ................................................................................25 2.4 Mean ( SE) channel catfish weight (kg) for media filled and DWC aquaponic systems .......................................................................................................32 2.5 Mean ( SE) channel catfish SGR (kg) for media filled and DWC aquaponic systems .......................................................................................................33 2.6 Mean ( SE) aquaponic basil FLM (kg) for media filled and DWC hydroponic subsystems ................................................................................................34 2.7 Mean ( SE) aquaponic basil yield (kg/0.6027 m2) for media filled and DWC hydroponic subsystems ...............................................................................35 2.8 Mean ( SE) aquaponic basil TVB (kg) for media filled and DWC hydroponic subsystems ................................................................................................36 2.9 Mean ( SE) aquaponic basil height (cm) for media filled and DWC hydroponic subsystems ...............................................................................37 2.10 Mean ( SE) aquaponic basil AGR (cm/day) for media filled and DWC hydroponic subsystems .............................................................................38 2.11 Mean ( SE) hydroponic basil FLM (kg) for media filled and DWC hydroponic subsystems .............................................................................40 2.12 Mean ( SE) hydroponic basil yield (kg/0.6027 m2) for media filled and DWC hydroponic subsystems ........................................................41 2.13 Mean ( SE) hydroponic basil TVB (kg) for media filled and DWC hydroponic subsystems .............................................................................42 2.14 Mean ( SE) hydroponic basil height (cm) for media filled and DWC hydroponic subsystems .............................................................................43 2.15 Mean ( SE) hydroponic basil AGR (cm/day) for media filled and hydroponic subsystems .......................................................................................44 x
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