HYPOTENSIVE PEPTIDES SYMPOSIUM CHAIRMEN: F. Sic uteri and E.G. Erdos ORGANIZING COMMITTEE: N. Back, E.G. Erdos and F. Sicuteri Assistants to the Organizing Committee: G. Franchi and M. Fanciullacci HONORARY COMMITTEE G. G. ARCH! -Rector of University, Florence D. BOVET -Institute of Pharmacology, University of Sassari L. CALIFANO -Institute of General Pathology, University of Naples J.H.GADDUM * -Depatrment of Pharmacology, University of Edinburgh E. GREPPI -Department of Medicine, University of Florence E. KATCHALSKI -Weizmann Institute, Israel L. LAGORIO -Mayor of Florence A. LUNEDEI -Dean of the School of Medicine of the University of Florence D.H.MURRAY -Dean of the School of Pharmacy, Buffalo, State U. of N. Y . M. ROCHA e SILVA -Chairman, Dept. of Pharmacol. U. of S. Paulo, Ribeirao Preto, S. Paulo, Brazil * Deceased SPONSORED BY The State University of New York at Buffalo and The University of Florence HYPOTENSIVE PEPTIDES Proceedings of the International Symposium October 25-29, 1965, Florence, Italy Ervin G. Erdos Professor of Pharmacology University of Oklahoma School of Medicine Editor-in-Chief Nathan Back Professor of Biochemical Pharmacology State University of New York at Buffalo Federigo Sicuteri Professor of Medicine University of Florence and A nne F. Wilde New Haven, Connecticut Technical Editor t SPRINGER-VERLAG • BERLIN. HEIDELBERG • NEW YORK - 19• • ISBN-13: 978-3-642-94967-8 e-TSBN-13: 978-3-642-94965-4 DOT: 10.1007/978-3-642-94965-4 All rights, especially that of translation into foreign languages, reserved. It is also forbidden to reproduce this book, either whole or in part, by photomechanical means (photostat, microfilm and/or microcard) or by other procedure without written permission from Springer-Verlag New York Inc. Library of Congress Catalog Card Number 66-21083 Title No. 1379 @ 1966 by Springer-Verlag New York Inc. Softcover reprint of the hardcover 1st edition 1966 List of Contributors AKIMOTO, SETSUKO, Laboratory of Physiological Chemistry, Tokyo College of SCience, Tokyo, Japan AMARO, J., Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana AMUNDSEN, EGIL, Institute of Physiology, UniversityofOslo, Norway ANASTASI, A., Farmitalia Research Laboratories, Milan, Italy ARMSTRONG, DESIREE, Middlesex Hospital Medical School, University of London, England ATSUMI, T., Institute for Cardiovascular Diseases and Depart ment of MediCine, Tokyo Medical and Dental University, Tokyo, Japan AUNGST, C. W., Division of Medicine, Roswell Park Memorial Institute, Buffalo, New York AUSTEN, K. FRANK, Massachusetts General Hospital, Boston, Massachusetts BACK, N., Department of Biochemical Pharmacology, The state University of New York at Buffalo, School of Pharm acy, Buffalo, New York BAROOS, T., The state University of New York at Buffalo, School of Pharmacy, Buffalo, New York BARLOW, B., The state University of New York at Buffalo, School of Pharmacy, Buffalo, New York BARROSO, JADER, Departamento de Bioquimica, Faculdade de Medicina, Universidade de Minas GeraiS, Belo Horizonte, Brazil BAUER, G., Pharmakologisches Institut der Universitiit Inns bruck, Austria BEACHAM, J., The Robert Robinson and Physiological Labora tories, Liverpool University, England BENTLEY, P. H., The Robert Robinson and Physiological Lab oratories, Liverpool University, England BERETTA ANGUISSOLA, A., Institute of Medical Pathology and Clinical Methodology, University of Turin, Italy BERNARDI, L., Istituto Ricerche Farmitalia, Milan, Italy v vi LIST OF CONTRIBUTORS BERT, G., Institute of Medical Pathology and Clinical Meth odology, University of Turin, Italy BERTACCINI, GIU LIO , Institute of Pharmacology, University of Parma, Italy BOBBIN, R., Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana BOREHAM, P. F. L., Nuffield Institute of Comparative Medi cine, The Zoological Society of London, England BORGHETTI, A., Instituto de Patologia Speciale Medica, Uni versita di Parma, Italy CAMPUS, S., Institute of Medical Pathology and Clinical Meth odology, University of Turin, Italy CANO, G., Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana CEI, J. M., Institute of Biology, National University of Cuyo, Mendoza, Argentina CHIANDUSSI, L., Institute of Medical Pathology and Clinical Methodology, University of Turin, Italy COLLIER, H. 0. J., Department of Pharmacological Research, Parke-Davis & Co., Hounslow, Middlesex, England CRAIG, LYMAN C., The Laboratories of The Rockefeller Uni versity, New York, New York DE CARO, G., Istituto di Patologia Speciale Medica, University of Parma, Italy DEL BIANCO, P. L., Center for the Study of Headaches, Gen eral Medical Clinic of the University of Florence, Italy Dl MATTEI, PIETRO, Institute of Pharmacology, University of Rome, Italy DINIZ, CARLOS R., Departamento de Bioquimica, Faculdade de Medicina, Universidade de Minas Gerais, Belo Hori zonte, Brazil DYER, DONALD C., Department of Pharmacology, Kansas University Medical Center, Kansas City, Kansas EDERY, H., Israel Institute for Biological Research, Ness Ziona, Israel EISEN, V., Department of Pharmacology, The Middlesex Hos pital Medical School, London, England ERDOS, ERVIN G., Department of Pharmacology, University of Oklahoma School of Medicine, Oklahoma City, Oklahoma ERSPAMER, V., Institute of Pharmacology of the University of Parma, Italy FANCIULLACCI, M., Center for the Study of Headaches, Gen eral Medical Clinic of the University of Florence, Italy LIST OF CONTRIBUTORS vii FERREffiA, S. H., Department of Pharmacology, Faculty of Medicine, University of sao Paulo, Ribeirao Preto, sao Paulo, Brazil FERUGLIO, F. S., Institute of Medical Pathology and Clinical Methodology, University of Turin, Italy FRANCHI, G., Center for the study of Headaches, General Medical Clinic of the University of Florence, Italy FREGNAN, G. B., Farmitalia Research Laboratories, Milan, Italy FRITZ, H., Klinisch-Chemisches Institut an der Chirurgischen Klinik der Universitat Miinchen, Germany FUJITA, T., Institute for Cardiovascular Diseases and Depart ment of Medicine, Tokyo Medical and Dental University, Tokyo, Japan FUKUSHIMA, HffiOKO, Laboratory of Physiological Chemistry, Tokyo College of Science, Tokyo, Japan FURANO, E., Laboratory of Clinical Biochemistry, National Heart Institute, Bethesda, Maryland GASPARRI, F., Istituto di Clinica Ostetrica della Universita degli studi di Firenze, Florence, Italy GLADNER, J. A., Laboratory of Biophysical Chemistry, Na tional Institute of Arthritis and Metabolic Diseases, Be thesda, Maryland GLASSER, A. H., Farmitalia Research Laboratories, Milan, Italy GOODWIN, L. G., Nuffield Institute of Comparative MediCine, The Zoological SoCiety of London, England GREENBAUM, LOWELL M., Department of Pharmacology, College of PhYSicians and Surgeons, Columbia University, New York, New York GREGORY, R. A., The Robert Robinson and Physiological Laboratories, Liverpool University, England GREPPI, E., Clinica Medica dell' Universita, Florence, Italy GUTH, P. S., Department of Pharmacology, Tulane University School of MediCine, New Orleans, Louisiana HABER, EDGAR, Department of Medicine, Harvard Medical School, Boston, Massachusetts HABERMANN, E., Institut fUr Pharmakologie der Universitiit, Wlirzburg, Germany HAEFELY, W., Department of Experimental MediCine, F. Hoff mann-La Roche & Co. Ltd., Basle, Switzerland HAUGE, A., Institute of PhYSiology, University of Oslo, Nor way viii LIST OF CONTRIBUTORS HEIZMANN, A., Department of Pharmacology, University of TUbingen, Germany HILTON, S. M., Department of Physiology, University of Birm ingham, England HOCHSTRASSER, K., Klinisch-Chemisches Institut an der Chir urgischen Klinik der Universitat Miinchen, Munich, Ger .. many HURLIMANN, A., Department of Experimental Medicine, F. Hoffmann-La Roche & Co. Ltd., Basle, Switzerland ISHIOKA, T., Institute for Cardiovascular Diseases and De partment of Medicine, Tokyo Medical and Dental Univer sity, Tokyo, Japan IW ANA GA, SADAAKI, The Institute for Protein Research, Osaka University, Osaka, Japan KA TCHALSKI, EPHRAIM, Department of Biophysics, Weizmann Institute of Science, Rehovoth, Israel KEELE, C. A., Department of Pharmacology, The Middlesex Hospital Medical School, London, England KENNER, G. W., The Robert Robinson and Physiological Laboratories, Liverpool University, England KONZETT, H., Pharmakologisches Institut der Universitiit Innsbruck, Austria KRIVOY, W., Department of Pharmacology, Baylor University College of MediCine, Texas Medical Center, Houston, Texas KROEGER, D. C., Department of Pharmacology, University of Texas Dental Branch, Houston, Texas LA GRECA, G., Istituto di Farmacologia dell' Universita di Parma, Italy LANZ, P., F. Hoffmann-LaRoche & Co. Ltd., Basle, Switzerland LEMBECK, F., Department of Pharmacology, University of Tiibingen, Germany LUBKE, KLAUS, ScheringAG, Hauptlaboratorium, Berlin, West Germany LUNDE, P. K. M., Institute of Physiology, University of Oslo, Norway MAC LEOD, J. K., The Robert Robinson and Physiological Laboratories, Liverpool University, England MARES-GUIA, MARCOS, Departamento de Bioquimica, Facul..; dade de Medicina, Universidade de Minas Gerais, Belo Horizonte, Brazil MARGOLIS, J., Children's Medical Research Foundation, Royal Alexandra Hospital for Children, Camperdown, New South Wales, Australia LIST OF CONTRIBUTORS ix MEINARDI, HARRY, The Laboratories of The Rockefeller University, New York, New York MELMON, KENNETH L., Departments of Medicine and Phar macology, The Section of Clinical Pharmacology and Car diovascular Research Institute, University of California, San Francisco, California MERRIFIELD, R. B., The Rockefeller University, New York, New York MILLS, G. L., The Middlesex Hospital Medical School, Uni versity of London, England MORIWAKI, CHIAKI, Laboratory of Physiological Chemistry, Tokyo College of SCience, Tokyo, Japan MORIYA, HIROSHI, Laboratory of Physiological Chemistry, Tokyo College of SCience, Tokyo, Japan NAGASAWA, SHIGEHARU, The Institute for Protein Research, Osaka University, Osaka, Japan NUMANO, F., Institute for Cardiovascular Diseases and De partment of MediCine, Tokyo Medical and Dental University, Tokyo, Japan OATES, JOHN A., Departments of Medicine and Pharmacology, The Division of Clinical Pharmacology, Vanderbilt Uni versity, Nashville, Tennessee PANDOLFO, G., Institute of Medical Pathology and Clinical Methodology, University of Turin, Italy PEREIRA, ANIBAL A., Departamento de Bioquimica, Facul dade de Medicina, Universidade de Minas Gerais, Belo Horizonte, Brazil PERITI, P., Istituto di Farmacologia della Universita degli studi di Firenze, Florence, Italy PIERCE, JACK V., Laboratory of MetabOlism, National Heart Institute, Bethesda, Maryland PISANO, J. J., Laboratory of Clinical Biochemistry, National Heart Institute, Bethesda, Maryland PRADO, J. L., Escola Paulista de Medicina, Sao Paulo, Brazil SARDESAI, V. M., The Robert S. Marx Laboratories of the Department of Surgery, Wayne state University School of Medicine, Detroit, Michigan SATO, TADASHI, The Institute for Protein Research, Osaka University, Osaka, Japan SCHACHTER, M., Department of PhYSiology, University of Alberta, Edmonton, Alberta, Canada SCHRODER, E., Schering AG, Hauptlaboratorium, Berlin, West Germany x LIST OF CONTRIBUTORS SEIDEL, G., Department of Pharmacology, University of TUbingen, Germany SHEPPARD, R. C., The Robert Robinson and Physiological Laboratories, Liverpool University, England SHIMAMOTO, T., Institute for Cardiovascular Diseases and Department of Medicine, Tokyo Medical and Dental Uni versity, Tokyo, Japan SICUTERI, F., Center for the study of Headaches, General Medical Clinic of the University of Florence, Italy SPRAGG, JOCELYN, Department of Medicine, Harvard Medical School, Boston, Massachusetts STEGER, R., The Roswell Park Memorial Institute, Buffalo, New York STERN, P., Department of Pharmacology, Medical Faculty, Sarajevo, Yugoslavia STEWART, JOHN MORROW, The Rockefeller University, New York, New York STORMER, E., Biological and Medical Research Division, Sandoz Ltd., Basle, Switzerland SUZUKI, TOMOJI, Institute for Protein Research, Osaka Uni versity, Osaka, Japan TAMURA, Z., Laboratory of Clinical Biochemistry, National Heart Institute, Bethesda, Maryland THAL, A. P., The Robert S. Marx Laboratories of the De partment of Surgery, Wayne state University School of Medicine, Detroit, Michigan THOENEN, H., Department of Experimental Medicine, F. Hoff mann-La Roche & Co. Ltd., Basle, Switzerland TRAUTSCHOLD, 1., Klinisch-Chemisches Institut an der Chir urgischen Klinik der Universitiit Miinchen, Munich, Ger many TSUKADA, G. A., The state University of New York at Buffalo, School of Pharmacy, Buffalo, New York UDENFRIED, S., Laboratory of Clinical Biochemistry, National Heart Institute, Bethesda, Maryland VICK, ROBERT L., Department of Physiology, Baylor Univer sity College of Medicine, Texas Medical Center, Houston, Texas VOGLER, K, F. Hoffmann-La Roche & Co. Ltd., Basle, Swit zerland VOGT, W., Department of Pharmacology, Max-Planck Institut fur Experimentelle Medizin, GOttingen, Germany