HUMIC ACID IN IMPROVING THE EFFICIENCY OF ORGANIC WASTES BY SHREEMATHI.P B.Sc. (Ag.) THESIS SUBMITTED TO THE PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN AGRICULTURE (ENVIRONMENTAL SCIENCE AND TECHNOLOGY) CHAIRPERSON: Dr. K. JEEVAN RAO DEPARTMENT OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY COLLEGE OF AGRICULTURE RAJENDRANAGAR, HYDERABAD – 500 030 PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSIRTY 2015 1 DECLARATION I, SHREEMATHI.P, hereby declare that the thesis entitled “HUMIC ACID IN IMPROVING THE EFFICIENCY OF ORGANIC WASTES” submitted to the Professor Jayashankar Telangana State Agricultural University for the degree of MASTER OF SCIENCE IN AGRICULTURE in the major field of ENVIRONMENTAL SCIENCE AND TECHNOLOGY is the result of original research work done by me. I also declare that no material contained in the thesis has been published earlier elsewhere in any manner. Place: Hyderabad (SHREEMATHI.P) Date: I.D. No. RAM/13-98 2 CERTIFICATE Ms. SHREEMATHI.P has satisfactorily prosecuted the course of research and that the thesis entitled “HUMIC ACID IN IMPROVING THE EFFICIENCY OF ORGANIC WASTES” submitted is the result of original research work and is of sufficiently high standard to warrant its presentation to the examination. I also certify that neither the thesis nor its part thereof has been previously submitted by her for a degree of any University. (Dr. K.JEEVAN RAO) Date: Chairperson 3 CERTIFICATE This is to certify that the thesis entitled “HUMIC ACID IN IMPROVING THE EFFICIENCY OF ORGANIC WASTES” submitted in partial fulfillment of the requirements for the degree of ‘MASTER OF SCIENCE IN AGRICULTURE’ (ENVIRONMENTAL SCIENCE AND TECHNOLOGY) of the PROFESSOR JAYASHANKARTELANGANA STATE AGRICULTURAL UNIVERSITY, Hyderabad is a record of the bonafide original research work carried out by Ms.SHREEMATHI.P under my guidance and supervision. The subject of the thesis has been approved by the Student’s Advisory Committee. No part of the thesis has been submitted by the student for any other degree or diploma. The published part and all assistance received during the course of investigations have been duly acknowledged by the author of the thesis. Thesis approved by the student’s advisory committee Chairperson Dr. K. Jeevan Rao Professor &University Head (FAC) Department of Environmental Science & Technology (Signature) College of Agriculture, Rajendranagar, Hyderabad-500030 Member Dr. B.Anil Kumar Assistant Professor Department of Environmental Science & Technology (Signature) College of Agriculture, Rajendranagar, Hyderabad-500030 Member Dr. S. Harish Kumar Sharma Associate Professor Department of Soil Science &Agril. Chemistry (Signature) College of Agriculture, Rajendranagar, Hyderabad-500030 Date of final viva-voce: 4 ACKNOWLEDGEMENTS My deep sincere thanks to the almighty Pilayar for his grace showered on me. With immense pleasure, I wish to express my heartfelt thanks and affectionate gratitude to my beloved mother Mrs. Meenatchi and father Mr. Parthiban whose everlasting love, unfading faith, incessant inspiration, moral and financial support and blessings kept me enthusiastic throughout my life and molded me to the present position, my dear brothers Balu anna, Dhanapal anna and my dear sister Poornima whose constant encouragement brings out the best in every one of my endeavors and without which this work could not be completed. I humbly consider it a privilege to have worked under esteemed guidance of Dr. K. Jeevan Rao, Professor and Principal Investigator, Department of Environmental Science and Technology, College of Agriculture, Rajendranagar and Chairman of my Advisory Committee. I place on records and my deep sense of gratitude to him for encouraging me to take up this research work and providing necessary guidance during the course of my investigation, because of which I have been able to complete the work assigned to me. I am equally and deeply indebted to Dr. P. Prabhu Prasadini, Professor, Department of Soil Science and Agricultural Chemistry, College of Agriculture, Rajendranagar, Hyderabad and Member of Advisory Committee for her valuable suggestions, encouragement, affection, personal guidance, keen interest, immeasurable help and constructive criticism given throughout my work and making it possible to bring out this thesis. It was an honour and privilege for me to be associated with her who stood by me with boundless patience in many difficult situations during my M. Sc. Degree Programme. I owe my effusive thanks to Dr. B. Anil Kumar, Assistant Professor, Department of Environmental Science and Technology, College of Agriculture, Rajendranagar, Hyderabad and Member of Advisory Committee for his encouragement, co-operation and valuable counsel, note-worthy guidance and cordial co-operation during the course of investigation. I am deeply thankful to Dr. D. Jagdishwar Reddy, Dept. of Environmental Science and Technology, for his constant encouragement, guidance and standing by me during 5 difficulties and helped in solving those during my research work, due to which, which I could complete my work in possible shortest time. I am extremely thankful to Dr. Baby Akula, Dr. V. Sailaja, Dr. Sridevi in the Department of Environmental Science and technology, Soil Science and Agricultural Chemistry, College of Agriculture, Rajendranagar for their valuable suggestions and whole-hearted help during my course work as well as research work. I wish to make a special mention for the help rendered by Mrs. Anuradha, senior research fellow and computer staff Mr. Praveen Mudiraj during research work for which I am highly thankful to both, my sincere thanks to my seniors K. Bhavya, Archana Karuni, Latika Devi, V. Rambabu Nayak, Praveen Solanki and to my classmates and friends Susan Priyadarshini, Vijay Kumar, Ramya and non-technical staff Neermala and Padma for their timely assistance and co-operation and all those who directly and indirectly helped me in the Department of Environmental Science & Technology during my study period and research work. Diction is not enough to express love and affection to my late grandfather and grandmother whose blessings gave me moral support without which I could not have completed the thesis. My brothers Pavithiran, Karthick, Dinesh, Raguram ranjan, Arutselvan, Suresh verma, sister Surya, Devadarshini, Karthiga and my nephew Srinithish whose affection and encouragement have been the source of inspiration during the course of my study. Uncles Kumaresan, Rajendran, Thiruvenkadam, Arumugam, Eakambaram, Venkat, Thangamani and aunt Selvi, Jayalakshmi, Viji, Tamil Ozhi and their children, brother-in-law Sankar. Words are not enough to express my wholehearted and affectionate gratitude to my friends Gomathi, Shyla, Deebika, Saranya, Gunavathi, Brindha, Kalaivanan, Gobu, Suresh Kumar, Suresh Kannan, Myilvaganan, Madhumitha, Latha for their unbounding love, unparallel affection and unstinted encouragement throughout my educational career. Finally, I wish to extend my thanks to all my dear relatives and all who have contributed even in a small way in the completion of my research work. (SHREEMATHI.P) 6 LIST OF CONTENTS CHAPTER No. TITLE PAGE No. I INTRODUCTION II REVIEW OF LITERATURE III MATERIAL AND METHODS IV RESULTS AND DISCUSSION V SUMMARY AND CONCLUSIONS LITERATURE CITED 7 LIST OF TABLES TABLE PAGE TITLE No. No. 1. Salient characteristics of water used in the experiment 2. Salient characteristics of experimental soil Allowable limits for pollutant concentration (ppm) of biosolids 3. for land application Salient characteristics of organic wastes (namely Urban 4. compost, Sewage sludge, Biochar and Humic acid) Effects of different treatments on fruits quality parameters, dry 5. matter production of plants and fruits, yield Effect of different treatments in plant contents (%) and nutrient 6. uptake (g plant-1) Effect of different treatments on micronutrients and heavy 7. metals content in plants (g plant-1) Effect of different treatments on Micronutrients and heavy 8. metals uptake by plants (g plant-1) Effect of different treatments in fruit content (%) and nutrient 9. uptake (g plant-1) Effect of different treatments on micronutrients and heavy 10. metals concentration in fruits (g plant-1) Effect of different treatments on micronutrients and heavy 11. metals uptake in fruits (g plant-1) Effect of different treatments on pH, EC (dS m-1), organic 12. carbon (%), available N,P,K (kg ha-1) of soil Effect of different treatments on micronutrients and heavy 13. metals in soil (mg kg-1) 8 LIST OF ILLUSTRATIONS Page Title S. No. Figure No. No. 1. 1 General view of pot culture experiment 2. 2 Harvesting of fruits after 90 days Micronutrients and heavy metals in organic wastes and 3. 3 humic acid (mg kg-1) Effect of different treatments on dry matter production and 4. 4 yield (g plant-1) Effect of different treatments on fruit quality parameters 5. 5 (mg 100g-1) 6. 6 Effect of different treatments on plant contents N, P, K (%) Effect of different treatments on plant nutrients N, P, K 7. 7 uptake (g plant-1) Effect of different treatments on micronutrient content in 8. 8 plants (g plant-1) Effect of different treatments on heavy metal contents in 9. 9 plants (g plant-1) Effect of different treatments on micronutrient uptake in 10. 10 plants (g plant-1) Effect of different treatments on heavy metals uptake in 11. 11 plants (g plant-1) Effect of different treatments on nutrient content in fruits 12. 12 (%) Effect of different treatments on nutrient uptake in fruits (g 13. 13 plant-1) Effect of different treatments on micronutrient content in 14. 14 fruits (g plant-1) Effect of different treatments on heavy metals content in 15. 15 fruits (g plant-1) Effect of different treatments on micronutrients uptake by 16. 16 fruits (g plant-1) 9 Page S. No. Figure No. Title19. No. Effect of different treatments on heavy metals uptake by 17. 17 fruits (g plant-1) Effect of different treatments on pH, EC, organic carbon 18. 18 content in soils. Effect of different treatments on major nutrient (N, P, K) in 19. 19 soils kg ha-1. Effect of different treatments on micronutrients in soils (mg 20. 20 kg-1). Effect of different treatments on heavy metal contents in 21. 21 soils (mg kg-1). 10
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