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Human Monoclonal Antibodies: Methods and Protocols PDF

367 Pages·2014·4.47 MB·English
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Methods in Molecular Biology 1060 Michael Steinitz Editor Human Monoclonal Antibodies Methods and Protocols M M B ™ ETHODS IN OLECULAR IOLOGY Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK For further volumes: h ttp://www.springer.com/series/7651 Human Monoclonal Antibodies Methods and Protocols Edited by Michael Steinitz Department of Pathology, The Lautenberg Center, IMRIC, The Hebrew University, Hadassah Medical School, Jerusalem, Israel Editor Michael Steinitz Department of Pathology The Lautenberg Center, IMRIC The Hebrew University Hadassah Medical School Jerusalem, I srael ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-62703-585-9 ISBN 978-1-62703-586-6 (eBook) DOI 10.1007/978-1-62703-586-6 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2013943914 © Springer Science+Business Media, LLC 2 014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifi cally for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is a brand of Springer Springer is part of Springer Science+Business Media (www.springer.com) Pref ace There is no doubt that the introduction of monoclonal antibodies revolutionized immunology. The ease and reproducibility of the various techniques for preparing murine monoclonal antibodies, using both cellular and molecular techniques, have had a major impact on their rapid and comprehensive expansion. The development of human monoclonal antibodies has been inspired primarily by the enormous clinical benefi ts promised by these reagents, used as anti-infl ammatory reagents, anti-tumor reagents, and reagents for passive immuni- zation. Initially, some technical and ethical obstacles hampered the development of entirely human monoclonal antibodies, prompting the development of chimeric and humanized monoclonal antibodies. The development of an immune response against the mouse resid- ual fragment in these antibodies stressed the need for complete human antibodies. Today, the development of advanced cellular and molecular techniques enables the production of human antibodies with any required specifi city. Considering the rapid improvement in the methods involved in making human monoclonal antibodies, it is expected that their impact will gradually be of major importance in the clinical setting. This volume presents technical protocols of cellular and molecular methods for the production, purifi cation, and application of human monoclonal antibodies. In addition, this volume presents also a few review articles related to the topic of human monoclonal and polyclonal antibodies. Jerusalem, Israel Michael Steinitz v Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Human Monoclonal Antibodies: The Residual Challenge of Antibody Immunogenicity.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Herman Waldmann 2 Technical and Ethical Limitations in Making Human Monoclonal Antibodies (An Overview).. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Mark C. Glassy and Rishab Gupta 3 Therapeutic Human Monoclonal Antibodies in Inflammatory Diseases . . . . . . 37 Sotirios Kotsovilis and Evangelos Andreakos 4 Therapeutic Human Monoclonal Antibodies Against Cancer. . . . . . . . . . . . . . 61 Jamie Jarboe, Anumeha Gupta, and Wasif Saif 5 Polyclonal and Monoclonal Antibodies in Clinic . . . . . . . . . . . . . . . . . . . . . . . 79 Bharath Wootla, Aleksandar Denic, and Moses Rodriguez 6 Production of Human Monoclonal Antibodies by the Epstein–Barr Virus Method.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 Michael Steinitz 7 Humanization and Simultaneous Optimization of Monoclonal Antibody. . . . . 123 T. Kuramochi, T. Igawa, H. Tsunoda, and K. Hattori 8 Chimeric Antibodies. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Kohei Kurosawa, Waka Lin, and Kunihiro Ohta 9 Recombinant Genetic Libraries and Human Monoclonal Antibodies. . . . . . . . 149 Jarrett J. Adams, Bryce Nelson, and Sachdev S. Sidhu 10 Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial Cytoplasm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171 Lilach Vaks and Itai Benhar 11 Construction and Production of an IgG-Like Tetravalent Bispecific Antibody, IgG–Single-Chain Fv Fusion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 Dan Lu and Zhenping Zhu 12 Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215 André Frenzel, Jonas Kügler, Sonja Wilke, Thomas Schirrmann, and Michael Hust 13 Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice.. . . . . 245 Susana Magadán Mompó and África González-Fernández vii viii Contents 14 Phage Display Technology for Human Monoclonal Antibodies. . . . . . . . . . . . 277 Cecilia Deantonio, Diego Cotella, Paolo Macor, Claudio Santoro, and Daniele Sblattero 15 Antigen-Specific In Vitro Immunization: A Source for Human Monoclonal Antibodies. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 297 Kosuke Tomimatsu and Sanetaka Shirahata 16 Methods for Radiolabelling of Monoclonal Antibodies.. . . . . . . . . . . . . . . . . . 309 Vladimir Tolmachev, Anna Orlova, and Karl Andersson 17 Purification of Human Monoclonal Antibodies and Their Fragments. . . . . . . . 331 Thomas Müller-Späth and Massimo Morbidelli 18 Idiotype-Specific Intravenous Immunoglobulin (IVIG) for Therapy of Autoimmune Diseases.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 353 Miri Blank, Tomer Bashi, and Yehuda Shoenfeld Index.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 Contributors JARRETT J. ADAMS • Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto , Toronto , ON , Canada ; Banting and Best Department of Medical Research , University of Toronto , Toronto , ON , Canada ; Department of Molecular Genetics, University of Toronto , Toronto , ON , Canada EVANGELOS ANDREAKOS • Laboratory of Immunogenetics, Immunobiology Division, Center of Immunology and Transplantation, Biomedical Research Foundation of the Academy of Athens , Athens , Hellas , Greece KARL ANDERSSON • Unit of Biomedical Radiation Sciences and Rudbeck Laboratory , Ridgeview Instruments AB , Uppsala , Sweden TOMER BASHI • Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Tel-Aviv University , Tel-Aviv , Israel ITAI BENHAR • The George S. Wise Faculty of Life Sciences, Department of Molecular Microbiology and Biotechnology, Tel-Aviv University , Ramat Aviv , Israel MIRI BLANK • Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Tel-Aviv University , Tel-Aviv , Israel DIEGO COTELLA • Department of Health Sciences and Interdisciplinary Research Center for Autoimmune Diseases (IRCAD), University of Eastern Piedmont , Novara , Italy CECILIA DEANTONIO • Department of Health Sciences and Interdisciplinary Research Center for Autoimmune Diseases (IRCAD), University of Eastern Piedmont , Novara , Italy ALEKSANDAR DENIC • Departments of Neurology and Immunology, Mayo Clinic , Rochester , MN , USA ANDRÉ FRENZEL • Abteilung Biotechnologie Technische Universität Braunschweig, Institut für Biochemie, Biotechnologie und Bioinformatik , Braunschweig, Germany ÁFRICA GONZÁLEZ-FERNÁNDEZ • Directora del Centro de Investigación Biomédica (CINBIO) , Vigo , Pontevedra, Spain ; Catedrática de Inmunología Universidad de Vigo , Vigo , Pontevedra, Spain MARK C. GLASSY • Integrated Medical Sciences Association Foundation , San Diego, CA , USA ANUMEHA GUPTA • Tufts University School of Medicine , Boston , MA , USA RISHAB GUPTA • BioProbe International, Inc., and University of California , Los Angeles, CA , USA K. HATTORI • Research Division , Chugai Pharmaceutical Co., Ltd. , Gotemba, Shizuoka, Japan MICHAEL HUST • Abteilung Biotechnologie, Technische Universität Braunschweig, Institut für Biochemie, Biotechnologie und Bioinformatik , Braunschweig, Germany T. IGAWA • Research Division , Chugai Pharmaceutical Co., Ltd. , Gotemba, Shizuoka, Japan JAMIE JARBOE • Tufts cancer center, Tufts university school of medicine , Boston , MA , USA ix

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The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immuni
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