(Horticulture) In Floriculture, Medicinal and Aromatic Plants PDF
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“CHANGES IN CORM RESERVE COMPOSITION DURING DORMANCY, SPROUTING AND INITIAL PLANT DEVELOPMENT OF GLADIOLUS (Gladiolus x grandiflorus).” By AMIT LOHAR (H-2012-01-M) Thesis submitted to the Uttar Banga Krishi Viswavidyalaya In partial fulfillment Of the requirements for the degree of MMaasstteerr ooff SScciieennccee ((HHoorrttiiccuullttuurree)) IInn FFlloorriiccuullttuurree,, MMeeddiicciinnaall aanndd AArroommaattiicc PPllaannttss WHERE WISDOM IS FREE 2014 FACULTY OF HORTICULTURE UTTAR BANGA KRISHI VISWAVIDYALAYA PUNDIBARI, COOCHBEHAR WEST BENGAL-736165 DEPARTMENT OF FLORICULTURE, MEDICINAL AND AROMATIC PLANTS FACULTY OF HORTICULTURE UTTAR BANGA KRISHI VISWAVIDYALAYA Pundibari, Cooch Behar, West Bengal From: Phone: Dr. S. S. Gantait Mobile : e-mail: Assistant Professor Ref.no.: …………… Date............... CERTIFICATE This is to certify that the thesis entitled “CHANGES IN CORM RESERVE COMPOSITION DURING DORMANCY,GERMINATION AND INITIAL PLANT DEVELOPMENT OF GLADIOLUS (Gladiolus x grandiflorus L.)” submitted by Mr.Amit lohar (H-2012-01-M) for partial fulfillment of the requirements for the Degree of Master of Science (Horticulture) in Floriculture, Medicinal & Aromatic Plants of Uttar Banga Krishi Viswavidyalaya, Pundibari is a faithful and bonafide research work carried out under my personal supervision and guidance. The results of the investigation reported in this thesis have not so far been submitted for any other degree or diploma. The assistance and help received during the course of investigation have been duly acknowledged. Place: Pundibari, Cooch Behar Dated (Dr. S. S Gantait) Chairman Advisory committee UTTAR BANGA KRISHI VISWAVIDYALAYA Department of Floriculture, Medicinal and Aromatic Plants Faculty of Horticulture Pundibari-736165, Cooch Behar, West Bengal, India WHERE WISDOM IS FREE . APPROVAL OF EXAMINERS FOR THE AWARD OF THE DEGREE MASTER OF SCIENCE (HORTICULTURE) IN FLORICULTURE, MEDICINAL AND AROMATIC PLANTS This is to certify that the thesis entitled “CHANGES IN CORM RESERVE COMPOSITION DURING DORMANCY, SPROUTING AND INITIAL PLANT DEVELOPMENT OF GLADIOLUS (Gladiolus x grandiflorus L.).” submitted by Mr. AMIT LOHAR (H-2012-01-M) to the Uttar Banga Krishi Viswavidyalaya, Pundibari, in partial fulfillment of the requirements for the degree of Master of Science in Horticulture (Floriculture, Medicinal & Aromatic Plants) has been approved by the Student’s Advisory Committee after viva-voce on the same in collaboration with an external examiner on 22.07.2014. NAME DESIGNATION SIGNATURE Dr. S. S. Gantait Assistant Professor and Chairman, Advisory Committee, Dept, of Floriculture, Medicinal & ……………….. Aromatic plants, Faculty of Horticulture. Dr.D.Barman External Examiner,principal scientist, ………………… NRC for orchids Pakyong,Sikkim- 737106 Dr. S. Maitra Asssociate Professor and Head, Dept, of Floriculture, Medicinal & Aromatic plants, ……………… Faculty of Horticulture (Member, Advisory Committee). Dr. A. Sarkar Associate Professor, Department of Genetics & ………………. Plant Breeding (Member, Advisory Committee). Dr. D. Mukhopadhyay Associate Professor and Head, Dept, of Soil ……………….... Science and Agricultural chemistry (Member, Advisory Committee). DDeeddiiccaatteedd TToo MMyy BBeelloovveedd PPaarreennttss && FFaammiillyy Acknowledgement I would like to express the deepest appreciation to my honourable chairman of Advisory Committee Dr. S.S Gantait, Department of Floriculture, Medicinal and Aromatic plants, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalaya for suggesting the problem, invaluable and ceaseless guidance, precious counsel, unstinted help, sustained interest and constant encouragement throughout the course of investigation and constructive critics during the preparation of manuscript of the thesis which can never be forgotten. It is pleasure on my part to acknowledge the debt I owe to Dr. J.C Jana, Dean, Faculty of Horticulture, and Uttar Banga Krishi Viswavidyalaya. I avail myself at this moment to express my sincere gratitude to him for their whole hearted co-operation, necessary assistance and sustained interest and facilities in carrying out the investigations. I would also like to convey my gratefulness to, Dr. Soumen Maitra, Associate Professor (Head) Department of Floriculture, Medicinal and Aromatic plants, and Dr. Arup sarkar, Associate Professor, Department of Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya. I am really grateful to Dr. Dibyendu Mukhopadhyay Associate Professor, Department of Soil science and Agricultural chemistry, Uttar Banga Krishi Viswavidyalaya who are the respected members of my Advisory Committee for their learned and affectionate guidance, helpful criticism and intensive encouragement throughout the period of this investigation. I would also like to extend my sincere thanks to all the non-teaching staff of the Department Floriculture, Medicinal and Aromatic plants, the Library staffs of the University and the field workers of instructional farm, U.B.K.V., Pundibari for their help during my study. I am especially indebted to my senior Mr.Suresh kumar Mahato, Mr.Kaushik Dutta. Sachin Sharma, Prakash Yakulay Rai and Rocky Thoksom, for their uncounted and irreparable co-operation as and when required. I express my lot of thanks to Kamal dah, Abhisekh thapa ,Tshering lama and my mates Bikash, and Chitra, all junior and seniors of U.B.K.V for their sincere co- operation during the period of my experiment and writing of this manuscript. Finally, at this moment, I want to put a record that, I have not completed that work without the wishes, encouragement and inspiration from my family. My deepest regard and love places them at the top of my gratitude where ocean of blessings and wishes will bestow over my head silently but constantly throughout my life and waited patiently for the day I complete my M. Sc. Degree. Last but not the least; I would like to thank my family, my parents, for giving birth to me at the first place and supporting me spiritually throughout my life. Dated 15.07.2014 ...................... Pundibari Cooch Behar Amit lohar CONTENTS CHAPTER DESCRIPTION PAGE(s) I INTRODUCTION 1-4 II REVIEW OF LITERATURE 5-9 III MATERIAL AND METHODS 10-15 IV RESULTS AND DISCUSSION 16-39 V SUMMARY AND CONCLUSION 40 VI BIBLIOGRAPHY I-IV Department of Floriculture, Medicinal & Aromatic Plants Faculty of Horticulture Uttar Banga Krishi Viswavidyalaya Pundibari, Coochbehar ____________________________________________________________________________________________ __ TITLE: Changes in corm reserve composition during dormancy, sprouting and initial plant development of gladiolus. (Gladiolus x grandiflorus L.) Name of Student : AMIT LOHAR Registration No. : H-2012-01-M Chairman of Advisory Committee : Dr. S. S. Gantait Year : 2014 ____________________________________________________________________________________________ __ ABSTRACT Gladiolus is one of the commercially important flower crops belonging to the family Iridaceae. It is one of the most cultivated flower crops because of its growing versatility and portability, and its corm can be dried and preserved for long periods. During this period, high catabolism is considered to be the main cause of changes in quality. These changes, occurring during post-harvest storage, are principally heat production, transpiration, and sprouting and rotting. Dormancy of corms, which is defined as a temporary suspension of visible growth of the meristematic tissues of the sprouts, has a major impact on the storage of corms. The chemical energy produced during photosynthesis accumulates as corm reserves in multiple forms including carbohydrates, lipids and proteins. In general mobilization of corm reserves following sprouting is essential for the corm to complete new plant establishment and, also signals the start of a new life cycle. Thus, the investigation was carried out with four genotypes of gladiolus to Changes in corm reserve composition during different stages of gladiolus. The experimental design adopted was two factor complete randomised block design in which there were two factors having two different levels viz, Red Ginger, Sunset Jubilee, Pacifica and Little Prince for first one whereas and dormancy, sprouting and initial plant development for second factor. Observations estimated and recorded at different stages were reducing sugar and non reducing sugar (Nelson, 1944; Somogyi, 1945), Starch (Hassid and Neufeld, 1964), Total soluble protein (Lowry’s method 1951), Lipids (Becker et al., 1978), Total phenol content (Malick and Singh 1980), Ascorbic acid (Roe and Kuether 1943), Peroxidase (Sadasivam and Manickam 1996) and Polyphenoloxidase (Mayer et al., 1965). Total reducing sugar (2.97, 3.42, 3.09), non reducing sugar (1.63, 1.67, 1.17), starch (2.49, 2.62, 1.47) and lipids (0.97, 1.08, 0.64) levels showed increasing trend up to sprouting stage thereafter reverse trend was observed indicating their key role in sprouting of corms whereas contents like protein (1.92, 1.26, 2.20) and ascorbic acid (2.17, 1.17, 3.35) levels decreases as the sprouting progress and showed the opposite trend at plant development stage. Results suggest that significant changes took place in sugar metabolisms. Changes in starch content closely followed sugar changes in the corms and this might be due to starch were converted to sugars. Low levels of starch and sugars in the corms as concentration level, indicating continued export and conversion to starch. This result suggests that during dormancy to vegetative stage there is a rapid change in corm reserve compositions and these analyses provide novel insights into key regulatory triggers for sprout dormancy release in gladiolus corms and provide the potential for the development of biochemical markers for sprout initiation. Keywords: corms, dormancy, sprouting, sugar, starch, polyphenol oxidase LIST OF TABLES Table No. Title Page no 4.1 Reducing Sugar content [mg g-1(f.w.)] in gladiolus corm at 16 different stages 4.2 Non Reducing Sugar content [mg g-1(f.w.)] in gladiolus corm at 19 different stages 4.3 Starch content [mg g-1(f.w.)] in gladiolus corm at different stages 21 4.4 Protein content [mg g-1(f.w.)] in gladiolus corm at different stages 24 4.5 Lipid content [mg g-1(f.w.)] in gladiolus corm at different stages 27 4.6 Total Phenol content [mg g-1(f.w.)] in gladiolus corm at different 29 stages 4.7 Ascorbic acid (mg/g) content changes in gladiolus corm at 32 different stages 4.8 Peroxidase content (U g-1 F.W.) changes in gladiolus corm at 35 different stages 4.9 Polyphenol oxidase (U g-1FW) content changes in gladiolus corm 38 at different stages
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