Гистология = Histology

129 Pages·2018·16.025 MB·Russian

by  Большова, Е. И.

HISTOLOGY
Laboratory Work for Dental Students

Student____________________________________ group ____

Minsk BSMU 2018
МИНИСТЕРСТВО ЗДРАВООХРАНЕНИЯ РЕСПУБЛИКИ БЕЛАРУСЬ
БЕЛОРУССКИЙ ГОСУДАРСТВЕННЫЙ МЕДИЦИНСКИЙ УНИВЕРСИТЕТ
КАФЕДРА МОРФОЛОГИИ ЧЕЛОВЕКА

ГИСТОЛОГИЯ

HISTOLOGY

Лабораторный практикум для студентов-стоматологов

5-е издание, исправленное

Минск БГМУ 2018
УДК 611-018(076.5)(075.8)-054.6
ББК 28.06я73
Г51

Рекомендовано Научно-методическим советом университета в качестве
практикума 16.05.2018 г., протокол № 9

Авто р ы: Е. И. Большова, А. А. Артишевский, Н. А. Жарикова,
В. В. Китель, Ю. М. Мельниченко, В. В. Заточная

Р е це нз е нт ы: доц. Т. М. Студеникина; доц. Д. В. Хандогий

Гистология = Histology : лабораторный практикум для студен-
Г51 тов-стоматологов / Е. И. Большова [и др.]. – 5-е изд., испр. –
Минск : БГМУ, 2018. – 128 с.
ISBN 978-985-21-0084-7.
Содержит обучающие и контролирующие схемы, таблицы и задания для
зарисовки микропрепаратов. Первое издание вышло в 2014 году.
Предназначен для студентов 1–2-го курсов медицинского факультета
иностранных учащихся по специальности «Стоматология», обучающихся на
английском языке.

УДК 611-018(076.5)(075.8)-054.6
ББК 28.06я73

ISBN 978-985-21-0084-7 © УО «Белорусский государственный
медицинский университет», 2018
2
Topic: OBJEСTS AND METHODS
OF MODERN HISTOLOGY LITERATURE

1. Ross, M. H. Histology : a text and atlas : with correlated cell and
1. Tasks, objects and methods of modern histology.
molecular biology / M. H. Ross, P. Wojciech. 6th ed. P. 1–21.
2. Applied value of histology for medical and biological
sciences. The connection between them.

3. Basic stages in the preparation of histology slides:

a) tissue fixation, processing, embedding and sectioning

b) histological staining methods

4. Instruments and research methods in light microscopy.
The resolution of the light microscope.

5. Instruments and research methods in electron microscopy.
Correct tasks №№ ___________________________
The resolution of the electron microscope.

THE LESSON IS COMPLETED
Hystology is _______________________________________________

__________________________________________________________
Teacher ________________________________
__________________________________________________________
«____» ______________________
__________________________________________________________

__________________________________________________________

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Task 1. HISTOLOGY HOW TO USE A HISTOLOGY MICROSCOPE Task 2. LIVER AXOLOTL
MICROSCOPE
1. Place a clean histology slide on the stage. The histology
Stain: H&E
slide should be centered with specimen side up. Secure
Magnification: 80×, 400×
the histology slide in place using the mechanical stage clips.
The microscope should be started with the lowest power
objective in place.
2. Lower the objective to the closest point without crunching
the histology slide against the objective lens. The objective
lens should not touch the slide. Look through the oculars
and bring the histology slide into focus with the coarse

focus adjustment.

3. Adjust the eyepieces for your interpupillary distance. While

looking at the histology slide, move the eyepieces together

and apart until the histology image appears as one image.

4. Rotate the revolving nosepiece in order to change

the magnification. When switching to a higher power

magnification, only the fine focus knob should be used to

focus.

H&E. This is a standard histology stain. «H&E» stand

for hematoxylin and eosin.

Indicate:
Basophilic is a technical term relating to tissue

1. Eyepieces / Oculars
components that stain readily with basic dyes. Hematoxylin

2. Arm
can be thought of as a basic dye. It binds to acidic structures,

3. Objective lenses staining them blue to purple. Therefore, the nucleus stains

4. Illuminator blue.
Indicate:
5. Fine focus knob
Oxyphilic refers to the tissue components stainable with
1. Nucleus (basophilic)
6. Coarse focus knob
an acid dye; thus, the structure being stained is basic. Eosin is
2. Cytoplasm (oxyphilic)
7. Revolving nosepiece
an acidic dye. It stains basic structures pink to pink/orange/red.
8. Stage

4
Topic: BASICS OF CYTOLOGY. LITERATURE
CELL MORPHOLOGY
1. Kuznetsov, S. L. Histology, Cytology and Embryology (a course

of lectures) / S. Kuznetsov, T. Boronikina, N. Gorychkina. Moscow :
1. Definition of a «сell». Cell theory.
Medical Informational Agency, 2004. P. 5–23.
2. Cytoplasm: general morphofunctional characteristics, 2. Erlandsen, S. L. Color Atlas of Histology / S. L. Erlandsen,
J. E. Magney. St. Louis : Мosby Year Book, 1992. 196 p.
physicochemical properties of hyaloplasm.
3. Ross, M. H. Histology : a text and atlas : with correlated cell and
3. Nucleus and its components. Nuclear-cytoplasmic ratio.
molecular biology / M. H. Ross, P. Wojciech. 6th ed. P. 22–97.
4. Biological membranes, general composition, chemical 4. Material of lectures.

components.

5. Cell organelles: their classification, structure and functions.
6. Cell inclusions: classification, structure and functions.

7. Classification and structure of cell junctions.
Correct tasks №№ ___________________________
8. Cell cycle. Cell reproduction. The reactive properties of

the cells and their medical and biological properties.

THE LESSON IS COMPLETED

Teacher _____________________________

«____» ______________________

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Task 3. THE STRUCTURE AND FUNCTIONS OF CELL ORGANELLES

Indicate:
1. Cell Membrane
2. Nucleus
a) nuclear envelope
b) nuclear pore
c) nucleolus
3. Mitochondria
a) outer membrane
b) inner membrane
4. Synthesis of proteins
5. Endoplasmic reticulum
a) rough
b) smooth
6. Transport vacuole
7. Golgy apparatus
8. Peroxisomes
9. Exocytosis
10. Lysosome
11. Endocytosis
a) phagosome
b) рhagolysosome

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Task 4. CELL PLASMA MEMBRANE Task 5. CELL JUNCTIONS

Indicate:
1. Tight junctions (tight junctions are a pair of trans-

Indicate: membrane protein fused on outer plasma membrane)
1. Phospholipid molecule 2. Desmosome (molecular complexes of cell adhesion
a) hydrophilic phosphate head group of phospholipid proteins and linking proteins that attach the cell
b) fatty acid tail (×2) of phospholipid surface adhesion proteins to intracellular keratin
2. Glycolipid (a phospholipid with a carbohydrate chain attached to it) cytoskeletal filaments)
3. Glycoprotein (a protein with a carbohydrate chain attached to it) 3. Gap junction or nexus (connects the cytoplasm of
a) carbohydrate chain of glycoprotein two cells and are made up of proteins called
4. Cholesterol connexins. It directly connects the cytoplasm of two
5. Protein cells, which allows various molecules and ions to
6. Protein channel (pore) pass freely between cells

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Task 6. INCLUSIONS OF GLYCOGEN Task 7. SKIN PIGMENT CELLS Task 8. FAT INCLUSIONS
IN THE LIVER CELLS OF AXOLOTL IN THE LIVER CELLS

Stain: Best’s carmine Stain: nuclei stained with hematoxylin Stain: osmic acid-safranine
Magnification: 400× Magnification: 400× Magnification: 400×

Indicate: Indicate: Indicate:
1. Nucleus 1. Nucleus 1. Nucleus
2. Granules of glycogen 2. Granules of the pigment melanin 2. Fat drops in the cytoplasm of cells
in the cytoplasm

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TOPIC: BASES OF HUMAN EMBRYOLOGY LITERATURE

1. Kuznetsov, S. L. Histology, Cytology and Embriology (a course of
1. Concept of embryogenesis. Connections between
lectures) / S. Kuznetsov, T. Boronikina, N. Gorychkina. Moscow :
phylogeny and ontogeny.
Medical Informational Agency, 2004. P. 223–239.
2. Basic periods of human embryogenesis. 2. Erlandsen, S. L. Color Atlas of Histology / S. L. Erlandsen,
J. E. Magney. St. Louis: Мosby Year Book, 1992. 196 p.
3. Progenesis. Morphological and functional characteristic of
3. Singh, I. Textbook of Human Histology (with Colour Atlas and
mature human gametes. Practical Guide) / I. Singh. 6th ed. P. 24–31.
4. Material of lectures.
4. Fertilization and zygote formation.

5. Cleavage. Structure of the blastocyst.

6. Gastrulation: early stage (7–14 days). Implantation.

Transformations in embryoblast and trophoblast. Correct tasks №№____________________________

7. Gastrulation: late stage (14–17 days). Transfer from

histotrophic to hemotrophic nutrition.

8. Histogenesis and organogenesis. The differentiation of
THE LESSON IS COMPLETED
germinal layers and their derivatives.

9. Extraembryonic organs: sources of development and
Teacher ____________________________
functions.
10. Placenta. Structure and function. «____» ______________________

11. Critical periods in human development.

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