Heterosis and Combining Ability Analysis to Leaf Curl Virus in Chilli DARSHAN S.

178 Pages·2017·5.99 MB·English

Heterosis and Combining Ability Analysis to
Leaf Curl Virus in Chilli

DARSHAN S.
(2012-11-176)

DEPARTMENT OF PLANT BREEDING AND GENETICS
COLLEGE OF AGRICULTURE
VELLAYANI, THIRUVANANTHAPURAM – 695 522
KERALA, INDIA
2014
Heterosis and Combining Ability Analysis to
Leaf Curl Virus in Chilli

by
DARSHAN S
.
(2012-11-176)

THESIS

Submitted in partial fulfillment of the
requirements for the degree of

MASTER OF SCIENCE IN AGRICULTURE
Faculty of Agriculture
Kerala Agricultural University

DEPARTMENT OF PLANT BREEDING AND GENETICS
COLLEGE OF AGRICULTURE
VELLAYANI, THIRUVANANTHAPURAM – 695 522
KERALA, INDIA
2014
ii

DECLARATION

I, hereby declare that the thesis entitled “Heterosis and Combining
Ability Analysis to Leaf Curl Virus in Chilli” is a bonafide record of research
done by me during the course of research and that the thesis has not previously
formed the basis for the award to me of any degree, diploma, fellowship or other
similar title, of any other University or Society.

Vellayani, DARSHAN. S.
Date: 08-08-14 (2012-11-176)
iii

CERTIFICATE

Certified that this thesis entitled “Heterosis and Combining Ability
Analysis to Leaf Curl Virus in Chilli” is a record of research work done
independently by Mr. Darshan S. under my guidance and supervision and that it
has not previously formed the basis for the award of any degree, diploma,
fellowship or associateship to him.

Vellayani, Mrs. Seeja, G.
Date: 08-08-14 (Major Advisor, Advisory Committee)
Assistant Professor (Sr.Scale)
Department of Plant Breeding and Genetics
College of Agriculture, Vellayani.
iv
CERTIFICATE

We, the undersigned members of the advisory committee of Mr. Darshan S
(2012-11-176), a candidate for the degree of Master of Science in Agriculture,
with major in Plant breeding and genetics, agree that this thesis entitled
“Heterosis and Combining Ability Analysis to Leaf Curl Virus in Chilli” may
be submitted by Mr. Darshan S., in partial fulfilment of the requirement for the
degree.

Mrs. Seeja, G.
(Chairman, Advisory Committee)
Assistant Professor (Sr. Scale)
Department of Plant Breeding & Genetics
College of Agriculture, Vellayani
Thiruvananthapuram-695522

Dr. D. S. Radha Devi Dr. K. Umamaheshwaran
(Member, Advisory Committee) (Member, Advisory Committee)
Professor and Head Professor
Department of Plant Breeding & Genetics Department of Plant pathology
College of Agriculture, Vellayani, College of Agriculture, Vellayani
Thiruvananthapuram-695522 Thiruvananthapuram-695522

Dr. R. V. Manju Dr. Mareen Abraham
(Member, Advisory Committee) (Member, Advisory Committee)
Associate Professor Associate Professor
Department of Plant Physiology Department of Plant Breeding & Genetics
College of Agriculture, Vellayani College of Agriculture, Vellayani,
Thiruvananthapuram-695522 Thiruvananthapuram-695522

EXTERNAL EXAMINER
(Name and Address)
v
ACKNOWLEDGEMENT
“By the grace of God I am what I am”
Life is an unending process of finding and losing, living and loving,
learning and moving on. I am deeply thankful to God for all his blessings. I bow
my head before the mighty power for his eternal love and grace for having this
way in my life.
I learned a lot of lessons even beyond the classroom and the books from
Mrs. Seeja, G., Chairperson of the Advisory committee, Assistant Professor (Sr.
S), Department of Plant breeding and Genetics. I feel immense pleasure in
extending my heartfelt gratitude for her perfectness, kindness, forgivingness,
simplicity and concern for my welfare.
To, Dr. D.S. Radha Devi, Professor and Head, Department of Plant
breeding and Genetics for her authentic advices as an advisory member for
research work and valuable suggestions during the course of study.
I owe my immense gratitude with pleasure to Dr. R.V. Manju, advisory
member and Associate Professor, Department of Plant Physiology for her timely
advice, selfless help, critical suggestions during the course of study and unstinted
help extended in experimental analysis.
I owe my heartful gratitude to Dr. K. Umamaheshwaran, Member,
Advisory Committee, and Professor, Department of Plant pathology for his
enlightened guidance, keen interest and suggestion to embellish this study.
I express my sincere gratitude to Dr. Mareen Abraham, Member,
Advisory Committee and Associate Professor, Department of Plant Breeding &
Genetics for her valuable suggestions during the thesis preparation.
I feel happy to express my heartful feelings to Dr. K.M. Abdul Khader,
for his critical suggestions during the entire course of study.
To, Dr. Arya K, for the compassionate heart, inspiration, sharing
idealisms and supporting throughout the course of study.
vi
Words are insufficient to express my sincere thanks to all my professors.
Especially, Dr. P. Manju, for her wonderful and lively classes. To Dr. Maya
Devi, P. for her complete and whole hearted fidelity.
My deepest thanks to Dr. Sunny, K. Oommen, Dr. D. Wilson, Dr.
Lekha Rani and Dr. Jayalekshmi, V. G., for their charisma filled with best
thoughts and warmth.
To, Dr. C. Gokulapalan, the man with a difference and one of the best
guru I’ve met, thank for making all the things easier and for the criticism, timely
concern and being readily available for consultation always.
To, Dr. Suma Bai, for allotment of experimental plot which made my
work and her critical suggestions for thesis preparation.
To, non-teaching staff members of my department. Lekshmi Chechi,
Reshma Chechi, Divya Chechi and other research associates of Plant physiology
department, for their kind heartedness and helping nature.
Everything went well with the presence of my loving friends, Anju,
Manju, Raju and Henry during my study period.
I would like to express my deepest gratitude to loving brother and senior
Ashish who made my thesis worth.
Many thanks go to my seniors, Sreenivas, Gangadhar, Rajib Das, Ramling,
Ravi2, Vijayaraj, Mady, Abhijatha, Vinetha, Vidya Gowda, Lekshmi and Asha for
their loving support and valuable suggestions during my studies and research.
I feel happy to thank all my lovely batchmates, Loki, Jayasheela,
Hemanth, Prasanth, Pavan, Kishore, Jayanth, Nikil, Jacob, Sreejith, Dipin, Anees,
Safeer, Anjali, Anupama, Reshma, Annie, Karolin, Revoo, Pinto and junior
friends for their everlasting love and care.
Best friendship knows no distance and I want to thank my ever loyal
friends back home. To, Subbu, Suri, Jeevan, Gowri, Kiran, Bhavya and Ashwini.
To my dear Yogitha, you always bring comfort and sound advice when I badly
need one.
To, my ever loving guru Shivakumar, M. S. for lighting the flame within
me again and bringing me back to academics with love and care.
I sincerely acknowledge the Kerala Agricultural University for financial
support in the form of KAU Junior Research Fellowship during my studies.
vii
My heartful thanks are due to NBPGR (New Delhi), IIHR (Bengaluru) and
GBPUAT (Pantnagar) for providing the experimental seed material.
To my very reasons for living: Dad-Mr. Shambhu, mom-Mrs. Kamala, for
support and love. To my loving sisters Lavanya and Sowmya who have always
been so proud of me.
Above all, I am very greatful to the Eternal Power guiding me throughout
this course programme.

DARSHAN S.
viii
CONTENTS
Sl. No. Particulars Page No.
1 INTRODUCTION 01
2 REVIEW OF LITERATURE 04
3 MATERIALS AND METHODS 28
4 RESULTS 52
5 DISCUSSION 103
6 SUMMARY 124
7 REFERENCES 127
ABSTRACT 149
ix
LIST OF TABLES
Table
Page
No. Title
No.
1 Details of parents used as experimental material 29
2 List of cross combinations 31
3 Scoring index for chilli leaf curl virus disease symptom. 40
Mean performance of parents and crosses for different
4 61
characters
Analysis of variance (ANOVA) for various characters –
5 65
RBD
Heterosis (%) over mid-parent (RH), better-parent (HB)
6 and standard commercial variety (SH) for various 66
characters
7 ANOVA for combining ability 95
8 Genetic components of variance 96
9 gca effect of parents for all the characters 97
sca effect of crosses for all the characters
10 98
Analysis of variance – CRD (Experiment 3) - Leaf Curl
11 Virus disease incidence – Artificial screening 102
Overall comparison of parents and hybrids by standard
12 heterosis, gca effect and sca effect for various traits 122