Handbook of Vascular Biology Techniques PDF
Preview Handbook of Vascular Biology Techniques
Mark Slevin · Garry McDowell Editors Handbook of Vascular Biology Techniques Handbook of Vascular Biology Techniques Mark Slevin (cid:129) Garry McDowell Editors Handbook of Vascular Biology Techniques Editors Mark Slevin Garry McDowell School of Healthcare Science School of Healthcare Science Manchester Metropolitan University Manchester Metropolitan University Manchester , UK Manchester , UK ISBN 978-94-017-9715-3 ISBN 978-94-017-9716-0 (eBook) DOI 10.1007/978-94-017-9716-0 Library of Congress Control Number: 2015936145 Springer Dordrecht Heidelberg New York London © Springer Science+Business Media Dordrecht 2015 T his work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. T he use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. T he publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Springer Science+Business Media B.V. Dordrecht is part of Springer Science+Business Media (www.springer.com) The authors would like to dedicate this book to: (Mark-Asha Shakira Slevin) (GMcD: To Christine and Amy) Pref ace W ithin this book, a variety of techniques are described in detail pertaining to methods used in both basic and advanced vascular biology. Methodologies range from in vitro cell culture to in vivo manipulations, through cell signalling proteomics and genomics to patient imaging in disease. A number of novel, state-of-the-art methodologies are also included. Each chapter is fully inclusive, containing s ections on trouble shooting and additional notes/links thus ensuring the reader has suffi cient information to carry out the protocol without additional requirements. This book should appeal to students, researchers and medical professionals working in all vascular-linked fi elds such as cardio- and cerebro-vascular, cancer and dementia. Manchester, UK Mark Slevin Garry McDowell vii Contents Part I In Vitro Techniques 1 In Vitro Angiogenesis Assay: Endothelial Migration, Proliferation, and Tube Formation ........................................................ 3 Kazuhide Hayakawa , Anna Chun-Ling L iang , Changhong Xing , Eng H. Lo , and Ken Arai 2 Endothelial Cell Tube Formation on Basement Membrane to Study Cancer Neoangiogenesis ...................................... 13 Amelia Casamassimi , Filomena de Nigris , Concetta Schiano , and Claudio Napoli 3 Induction of Hypoxia in Vascular Endothelial Cell Culture ............... 23 Hyun-Young Koo , Meredith Millay , and Tsutomu Kume 4 Evaluating In Vitro Angiogenesis Using Live Cell Imaging ................ 29 Elen Bray and Mark Slevin 5 Isolation of Endothelial Progenitor Cells (EPCs)................................. 45 Aaron Liew and Timothy O’Brien 6 Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis ................................................. 55 Michael Carroll and Clare Nevin 7 Assessment of Vascular Function and Contractility, Ex Vivo .............. 65 May Azzawi 8 Measurement of Aβ Uptake by Cerebrovascular Smooth Muscle Cells ............................................................................... 81 Wan Adriyani Wan R uzali and Seth Love 9 Measurement of Intracellular Ca2 + in Human Endothelial Cells ..................................................................................... 95 Sarah Jones ix
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