Handbook of Media for Clinical Microbiology

542 Pages·2006·19.66 MB·English

by  James W. Snyder

Preface About the Authors
Almost 1,650 media are described in the second edi- Ronald M. Atlas is Graduate Dean, Professor of
tion of the Handbook of Media for Clinical Microbi- Biology, and Professor of Public Health at the Uni-
ology, including newly described media for the culti- versity of Louisville. He received his B.S. from the
vation of emerging pathogens. Diseases caused by State University of New York at Stony Brook
emerging pathogens that are responsible for increased in 1968 and his M.S. and Ph.D. from Rutgers in
rates of morbidity and mortality rates, such as Escher- 1970 and 1972, respectively. Dr. Atlas has received
ichia coli O157:H7, methicillin resistant Staphylococ- a number of honors including: The University of
cus aureus, and vancomycin resistant enterococci, Louisville Excellence in Research Award, the
have raised special concerns and various media Johnson and Johnson Fellowship for Biology, and
included in the Handbook have been designed for the the ASM Award in Applied and Environmental
specific cultivation and identification of these patho- Microbiology. He has authored several microbiology
gens. textbooks and handbooks of microbiological media.
His research has included development of diagnostic
Many of the new media included in the second edi-
systems for pathogenic microorganisms. He has
tion of the Handbook of Media for Clinical Microbi-
served on the editorial boards of Applied and Envi-
ology permit the cultivation of bacteria, fungi, and
ronmental Microbiology, BioScience, Biotechniques,
viruses that are currently causing major medical
Environmental Microbiology, Biosecurity, and Biot-
problems around the world. These media are very
errorism, and Journal of Industrial Microbiology.
important for the rapid detection of pathogenic
He is editor of Critical Reviews in Microbiology. He
microorganisms and the diagnosis of individuals
also has served as President of the American Society
with specific infectious diseases. Several of the new
for Microbiology.
media described in the second edition of the Hand-
book of Media for Clinical Microbiology include James W. Snyder is Professor of Pathology in the
chromogenic or fluorogenic substrates that permit Department of Pathology and Laboratory Medicine
the rapid detection of specific pathogens. at the University of Louisville School of Medicine
and serves as the Director of Microbiology for the
An important function of the second edition of the University of Louisville Hospital. He received his
Handbook of Media for Clinical Microbiology is to B.S. in 1969 and M.S. in 1970 from Eastern Ken-
provide descriptions of the media that are used to tucky University and Ph.D. in 1974 from the Univer-
cultivate and identify microorganisms from clinical sity of Dayton. Dr. Snyder is a diplomate of the
specimens and to maintain reference cultures of American Board of Medical Microbiology (ABMM)
human pathogens in various clinical settings. The and a Fellow in the American Academy of Microbi-
Handbook provides a compilation of the formula- ology. He has received a number of honors includ-
tions, methods of preparation, and applications for ing: The South Central Association for Clinical
media used in the clinical microbiology laboratory. Microbiology Outstanding Contribution to Microbi-
Each listing is alphabetical and includes medium ology Award, the Spirit of Louisville Foundation
composition, instruction for preparation, commer- Award, Outstanding Service Award as Chairman of
cial sources, and intended uses. the Committee on Continuing Education of the
American Society for Microbiology, and election to
The format of the Handbook allows easy reference
the Alpha Omega Alpha Honor Medical Society. He
to information needed to prepare media for the culti-
is the former Editor of The Yearbook of Clinical
vation of microorganisms relevant to clinical diag-
Microbiology and the Certified Clinical Microbiol-
nostics. The second edition of the Handbook of
ogy Data Base. He is currently a member of the
Media for Clinical Microbiology includes descrip-
Critical Reviews in Microbiology editorial board and
tions of expected results as they apply to microor-
serves on the Professional Affairs Committee of the
ganisms of importance for the examination of clini-
American Society for Microbiology. He maintains
cal specimens.
an active research program in both applied and basic
Importantly, the second edition of the Handbook of clinical microbiology, including molecular tech-
Media for Clinical Microbiology is user friendly and niques for the detection of pathogens and the assess-
should save time and effort for anyone cultivating ment and comparison of automated and nonauto-
pathogenic microorganisms. It should be a valuable mated diagnostic systems and tracking of microbial
resource for anyone working in the area of clinical incidence and in vitro activity of antibiotics in sup-
microbiology. port of local and national surveillance programs.

Table of Contents
Diagnostic Microbiology: Isolation Alginate Utilization Medium.................................21
and Identification of Pathogens...........................1 Alkaline Peptone Agar...........................................22
Isolation and Culture Procedures.............................1 Alkaline Peptone Salt Broth..................................22
Media for the Isolation and Identification Alkaline Peptone Water.........................................22
of Microorganisms from Clinical Specimens......2 Alkvisco Medium..................................................22
References................................................................4 Allantoin Agar.......................................................22
A 3 Agar...................................................................5 Allantoin Broth......................................................23
A 3B Agar................................................................5 ALOA Medium......................................................23
A 7 Agar...................................................................6 ALP Basal Medium...............................................24
A 7 Agar, Modified..................................................7 Amies Modified Transport Medium
A 7B Agar................................................................7 with Charcoal....................................................24
A 8B Agar................................................................8 Amies Modified Transport Medium
A 1 Broth.................................................................9 with Charcoal....................................................25
Abeyta-Hunt Bark Agar...........................................9 Amies Transport Medium without Charcoal.........25
ABY Agar..............................................................10 AMO.1 Medium....................................................25
AC Agar.................................................................10 Ampicillin Kanamycin Nutrient Agar...................26
AC Broth................................................................10 Ampicillin L Broth Medium..................................26
Acanthamoeba Medium.........................................11 Ampicillin TY Salt Medium..................................27
ACC Medium.........................................................11 Amygdalin Medium...............................................27
Acetamide Agar.....................................................11 Anaerobe Agar.......................................................27
Acetamide Broth....................................................11 Anaerobe Agar.......................................................28
Acetamide Cetrimide Glycerol Mannitol Anaerobe Medium.................................................28
Selective Medium..............................................12 Anaerobic Agar......................................................28
Acetamide Medium................................................12 Anaerobic Agar......................................................29
Acetamide Medium................................................13 Anaerobic Broth.....................................................29
Acetate Differential Agar.......................................13 Anaerobic CNA Agar............................................29
Acetobacteroides glycinophilus Medium...............13 Anaerobic Egg Yolk Agar......................................29
Achromobacter Choline Medium..........................14 Anaerobic Egg Yolk Agar......................................30
Achromobacter Choline Anaerobic LKV Blood Agar..................................30
Medium, Modified.............................................14 Anaerobic Oxalate Medium...................................31
Achromobacter Medium........................................15 Anaerobic Trypticase™ Soy Agar
Acid Egg Medium..................................................15 with Calf Blood.................................................32
Acid Tomato Broth................................................15 Anaerobic TVLS Medium.....................................32
Actidione® Agar....................................................15 Andersen’s Pork Pea Agar.....................................32
Actinomyces Agar..................................................16 Andrade’s Carbohydrate Broth and Indicator........33
Actinomyces Broth.................................................16 Andrade’s Broth.....................................................33
Actinomyces Isolation Agar...................................16 Anthracis Chromogenic Agar................................34
Actinomycete Growth Medium.............................17 Antibiotic Medium 1..............................................34
Activated Carbon Medium.....................................17 Antibiotic Medium 2..............................................34
AE Sporulation Medium, Modified.......................17 Antibiotic Medium 3..............................................34
Aeromonas Differential Agar.................................18 Antibiotic Medium 3 Plus......................................35
Aeromonas hydrophila Medium............................18 Antibiotic Medium 4..............................................35
Aeromonas Medium...............................................18 Antibiotic Medium 5..............................................35
AFPA......................................................................19 Antibiotic Medium 6..............................................35
AH5 Medium.........................................................19 Antibiotic Medium 7..............................................35
AKI Medium..........................................................20 Antibiotic Medium 8..............................................36
Alcaligenes Agar....................................................20 Antibiotic Medium 9..............................................36
Alcaligenes Medium..............................................20 Antibiotic Medium 10............................................36
Alcaligenes N5 Medium........................................20 Antibiotic Medium 11............................................36
Alcaligenes NA YE Medium.................................21 Antibiotic Medium 12............................................37
Alcaligenes NB YE Agar.......................................21 Antibiotic Medium 13............................................37
Alcaligenes NB YE Broth......................................21 Antibiotic Medium 19............................................37
Alcaligenes NB YE Medium.................................21 Antibiotic Medium 20............................................37
Antibiotic Medium 21............................................37 Beef Infusion Agar................................................55
Antibiotic Sulfonamide Sensitivity Test Agar.......38 Beef Infusion Broth...............................................56
AOAC Letheen Broth............................................38 Beef Liver Medium for Anaerobes........................56
Arenavirus Plaquing Medium................................38 Bennett’s Agar.......................................................56
Arginine Broth.......................................................39 Bennett’s Agar with Maltose.................................56
Arginine Broth with NaCl......................................39 Bennett’s Modified Agar Medium.........................56
Arginine Glucose Slants........................................39 BG Sulfa Agar.......................................................56
Armstrong Fusarium Medium...............................39 BHI with Glucose..................................................57
Arylsulfatase Agar.................................................39 BHI Glucose Medium............................................57
Ascospore Agar......................................................40 BHI with Glycerol and Reducing Agents..............57
ASM Medium........................................................40 BHI Medium..........................................................58
Asparaginate Glycerol Agar..................................40 BHI/1 Medium.......................................................58
Asparagine Broth...................................................40 BHI/2 Medium.......................................................58
Aspergillus Differential Medium...........................40 BHI/3 Medium.......................................................58
Aspergillus Medium...............................................41 Bifidobacterium Medium.......................................58
ATS Medium..........................................................41 BiGGY Agar..........................................................59
AV Agar with Vitamins..........................................41 Bile Esculin Agar...................................................59
Avian Mycoplasma Agar........................................42 Bile Esculin Agar with Kanamycin.......................60
Avian Mycoplasma Broth......................................42 Bile Esculin Azide Agar........................................60
Azide Blood Agar..................................................43 Bile Oxalate Sorbose Broth...................................60
Azide Blood Agar with Crystal Violet...................43 Bile Salts Brilliant Green Starch Agar...................61
Azide Broth............................................................44 Bile Salts Gelatin Agar..........................................61
Azide Medium.......................................................44 BIN Medium..........................................................62
B Broth...................................................................44 Biosynth Chromogenic Medium
Bacillus cereus Medium........................................44 for Listeria monocytogenes...............................62
Bacillus cereus Selective Agar Base......................45 Biphasic Medium for Neisseria.............................62
Bacillus Medium....................................................45 Bird Seed Agar......................................................63
Bacillus Medium....................................................46 Bismuth Sulfite Agar.............................................63
Bacteroides Bile Esculin Agar...............................46 Bismuth Sulfite Agar Wilson and Blair.................63
BAF Agar...............................................................47 Bismuth Sulfite Broth............................................64
BAGG Broth..........................................................47 BL Agar.................................................................64
Baird-Parker Agar..................................................47 Blood Agar.............................................................64
Baird-Parker Agar, Supplemented.........................48 Blood Agar Base....................................................65
Baird-Parker Medium............................................48 Blood Agar Base No. 2..........................................65
Balamuth Medium.................................................48 Blood Agar Base with Peptone..............................66
Basal Synthetic Medium........................................49 Blood Agar Base, Sheep........................................66
Basic Cultivation Medium.....................................49 Blood Agar Base with Special Peptone.................66
Basic Mineral Medium..........................................49 Blood Agar, Diphasic............................................66
BC Motility Medium.............................................49 Blood Agar, Diphasic Base Medium.....................67
BCM Bacillus cereus Group Plating Medium.......49 Blood Agar with Low pH......................................67
BCM O157:H7(+) Plating Medium.......................50 Blood Agar No. 2...................................................67
BCP D Agar...........................................................50 Blood Base Agar....................................................67
BCP DCLS Agar....................................................50 Blood Base Agar with Charcoal............................67
BCYE Agar............................................................50 Blood Base Agar with Horse Blood......................68
BCYE Differential Agar........................................51 Blood Base Agar with
BCYE Medium, Diphasic Blood Culture..............51 Horse Blood, Fumarate and Formate................68
BCYE Selective Agar with CCVC........................52 Blood Glucose Cystine Agar.................................68
BCYE Selective Agar with GPVA.........................52 BMPA-α Medium..................................................68
BCYE Selective Agar with GVPC........................53 BMPA-α Medium..................................................69
BCYE Selective Agar with PAC............................53 Bonner-Addicott Medium......................................69
BCYE Selective Agar with PAV............................54 Bordetella pertussis Selective Medium
BCYEα with Alb...................................................54 with Bordet-Gengou Agar Base........................69
BCYEα without L-Cysteine...................................54 Bordetella pertussis Selective Medium
Beef Extract Agar..................................................55 with Charcoal Agar Base...................................70
Beef Extract Broth.................................................55 Bordet-Gengou Agar.............................................70
Beef Extract Peptone Serum Medium...................55 Bordet-Gengou Medium........................................71
Borrelia Medium...................................................71 Brilliant Green 2%-Bile Broth,
Bouillon Medium...................................................72 Fluorocult®.......................................................84
Bovine Albumin Tween 80 Medium, Brilliant Green Lactose Bile Broth........................84
Ellinghausen and McCullough, Modified.........72 Brilliant Green Phenol Red Agar...........................84
Bovine Albumin Tween 80....................................80 BROLACIN MUG Agar........................................84
Semisolid Medium, Ellinghausen Bromcresol Purple Broth.......................................85
and McCullough, Modified...............................72 Bromcresol Purple Dextrose Broth........................85
Bovine Serum Albumin Tween 80 Agar................73 Bromthymol Blue Agar.........................................85
Bovine Serum Albumin Tween 80 Broth..............73 Bromthymol Blue Broth........................................86
Bovine Serum Albumin Tween 80 Soft Agar........74 Brucella Agar.........................................................86
Brain Heart CC Agar.............................................74 Brucella Agar Base Campylobacter Medium........87
Brain Heart Infusion..............................................74 Brucella Agar with Vitamin K .............................87
1
Brain Heart Infusion Agar.....................................75 Brucella Albimi Broth with 0.16% Agar...............87
Brain Heart Infusion Agar 0.7%............................75 Brucella Albimi Broth with 0.16%
Brain Heart Infusion with 0.7% Agar....................76 Agar and 1% Glycine........................................87
Brain Heart Infusion Agar with Brucella Albimi Broth
Chloramphenicol...............................................76 with Formate and Fumarate...............................88
Brain Heart Infusion Agar with Kanamycin..........76 Brucella Albimi Broth with Sheep Blood.............88
Brain Heart Infusion Agar Brucella Albimi Medium, Semisolid.....................88
with Penicillin and Streptomycin......................76 Brucella Anaerobic Blood Agar............................88
Brain Heart Infusion Agar with 10% Sheep Brucella Blood Agar with Hemin
Blood, Gentamicin, and Chloramphenicol........77 and Vitamin K .........................................................89
1
Brain Heart Infusion Agar with Tween 80.............77 Brucella Blood Culture Broth................................89
Brain Heart Infusion with Agar, Brucella Broth.......................................................89
Yeast Extract, NaCl, Inactivated Brucella Broth with Additives...............................90
Horse Serum, and Penicillin..............................77 Brucella Broth with 0.16% Agar...........................90
Brain Heart Infusion with Agar, Brucella Broth Base Campylobacter Medium......91
Yeast Extract, Sucrose, Brucella Broth, Modified.......................................91
Horse Serum, and Penicillin..............................78 Brucella FBP Agar................................................91
Brain Heart Infusion with Agar, Brucella FBP Broth...............................................91
Yeast Extract, Sucrose, Inactivated Brucella Medium Base..........................................92
Horse Serum, and Penicillin..............................78 Brucella Medium, Selective..................................92
Brain Heart Infusion Blood Agar...........................78 Brucella Selective Medium...................................92
Brain Heart Infusion Broth....................................79 Brucella Semisolid Medium
Brain Heart Infusion with Casein..........................79 with Cysteine.....................................................92
Brain Heart Infusion Casein Starch.......................79 Brucella Semisolid Medium with Glycine............93
Brain Heart Infusion with Cystine.........................79 Brucella Semisolid Medium with NaCl................93
Brain Heart Infusion Brucella Semisolid Medium with Nitrate..............93
with Glucose and Horse Serum.........................80 BSK Medium.........................................................94
Brain Heart Infusion with 5% NaCl......................80 BSK Medium, Modified........................................94
Brain Heart Infusion with PABA...........................80 BSK Medium, Revised..........................................95
Brain Heart Infusion BTB Lactose Agar.................................................96
with PABA and Agar.........................................80 BTB Teepol® Agar................................................96
Brain Heart Infusion with Rabbit Serum...............81 BTU Medium.........................................................97
Brain Heart Infusion with 3% Sodium Chloride...81 Buffered Charcoal Yeast Extract
Brain Heart Infusion Soil Extract Medium............81 Differential Agar...............................................97
Brain Heart Infusion with Sucrose Buffered Enrichment Broth...................................97
and Horse Serum...............................................82 Buffered Peptone Water.........................................98
Brain Liver Heart Semisolid Medium...................82 Burkholderia cepacia Agar....................................98
Brewer Anaerobic Agar.........................................82 BYE Agar..............................................................98
Brewer Thioglycolate Medium..............................82 BYEB.....................................................................99
Brewer Thioglycolate Medium, Modified.............82 Caffeic Acid Ferric Citrate Test Medium..............99
BRILA MUG Broth...............................................83 Caffeine Medium...................................................99
Brilliant Green Agar..............................................83 CAL Agar............................................................100
Brilliant Green Agar, Modified..............................83 CAL Broth...........................................................100
Calymmatobacterium granulomatis Cary and Blair Transport Medium.......................115
Semidefined Medium......................................100 Cary and Blair
CAMG Broth.......................................................100 Transport Medium, Modified..........................115
Campy THIO Medium.........................................100 Casamino Acids Yeast
Campylobacter Agar............................................101 Extract Lincomycin Medium..........................116
Campylobacter Agar with 5 Casamino Acids Yeast
Antimicrobics and 10% Sheep Blood.............101 Extract Salts Broth, Gorbach...........................116
Campylobacter Agar, Blaser’s.............................101 Casein Agar.........................................................116
Campylobacter Agar, Skirrow’s..........................102 Casein Yeast Extract Glucose Agar.....................116
Campylobacter Blood-Free Agar Casein Yeast Extract Glucose Broth....................117
Base Modified.................................................102 Casman Agar Base...............................................117
Campylobacter Blood-Free Casman Agar Base with Rabbit Blood................117
Selective Agar.................................................102 CASO MUG Agar...............................................117
Campylobacter Charcoal CCY Modified Medium.......................................118
Differential Agar.............................................103 CDC Anaerobe Blood Agar.................................118
Campylobacter Enrichment Broth.......................103 CDC Anaerobe Blood Agar
Campylobacter Enrichment Broth.......................104 with Kanamycin and Vancomycin...................118
Campylobacter fecalis Medium...........................105 CDC Anaerobe Blood Agar
Campylobacter fetus Medium..............................105 with Phenylethyl Alcohol................................119
Campylobacter fetus Medium..............................106 CDC Anaerobe Laked Blood Agar
Campylobacter fetus Selective Medium..............106 with Kanamycin and Vancomycin...................119
Campylobacter Isolation Agar A.........................106 Cefiximine Rhamnose Sorbitol
Campylobacter Isolation Agar B.........................107 MacConkey Agar............................................120
Campylobacter Medium......................................107 Cefsulodin Irgasan Novobiocin Agar..................120
Campylobacter mucosalis Medium.....................107 Cell Growth Medium...........................................121
Campylobacter rectus Medium............................108 Cellobiose Polymyxin
Campylobacter Selective B Colistin Agar, Modified...............................122
Medium, Blaser-Wang.....................................108 Cellobiose Polymyxin Colistin Agar...................123
Campylobacter Selective Cereal Agar..........................................................123
Medium, Blaser-Wang.....................................109 Cetrimide Agar, Non-USP...................................123
Campylobacter Selective Cetrimide Agar, USP...........................................123
Medium, Butzler’s...........................................109 CF Assay Medium...............................................123
Campylobacter Selective CFAT Medium.....................................................124
Medium, Karmali’s..........................................110 CGY Agar............................................................124
Campylobacter Selective Medium, CH 1 Medium......................................................124
Preston’s..........................................................110 Chapman Stone Agar...........................................125
Campylobacter sputorum Charcoal Agar......................................................125
Subspecies bubulus Medium...........................110 Charcoal Agar with Horse Blood........................125
Campylobacter sputorum Charcoal Agar with Horse Blood
Subspecies mucosalis Medium........................111 and Cepahalexin..............................................125
Campylobacter Thioglycolate Charcoal Blood Medium......................................126
Medium with 5 Antimicrobics........................111 Chlamydia Growth Medium................................126
Candida BCG Agar Base.....................................111 Chlamydia Isolation Medium..............................127
Candida Isolation Agar........................................112 Chlamydospore Agar...........................................127
Capnocytophaga Medium....................................112 Chloramphenicol Ampicillin LB Medium...........127
Capnocytophaga II Medium................................112 Chloramphenicol Erythromycin LB Medium......128
Carbohydrate Fermentation Broth.......................112 Chloramphenicol L Broth Medium No. 1............128
Carbon Assimilation Medium..............................113 Chloramphenicol L Broth Medium No. 2............128
Carbon Assimilation Medium, Chloramphenicol L Broth Medium No. 3............129
Auxanographic Method for Yeast Chloramphenicol LB Medium No. 1...................129
Identification...................................................113 Chloramphenicol LB Medium No. 2...................129
Carbon Utilization Test........................................114 CHO Medium Base..............................................129
Cardiobacterium hominis Medium......................114 Chocolate Agar....................................................129
Carnitine Chloride Medium.................................114 Chocolate Agar....................................................130
Carrot Potato Dextrose Agar................................115 Chocolate Agar, Enriched....................................130
Chocolate Agar, Enriched....................................131 Clostridium Medium............................................148
Chocolate Agar-Bartonella C-29.........................131 Clostridium Medium............................................149
Chocolate II Agar.................................................132 Clostridium novyi Blood Agar.............................149
Chocolate II Agar with Clostridium perfringens Agar, OPSP..................150
Hemoglobin and IsoVitaleX............................132 Clostridium Selective Agar..................................150
Chocolate Tellurite Agar......................................132 CM3 Medium.......................................................150
Cholera Medium TCBS.......................................133 CN Screen Medium.............................................151
Cholic Acid Medium...........................................133 Coagulase Agar Base...........................................151
Cholera Medium TCBS.......................................133 Coagulase Mannitol Agar....................................151
Chopped Meat Agar.............................................134 COBA..................................................................151
Chopped Meat Broth............................................134 Coletsos Medium.................................................152
Chopped Meat Broth with Carbohydrates...........134 Coletsos Selective Medium.................................152
Chopped Meat Broth Coliform Agar, Chromocult®..............................153
with Formate and Fumarate.............................134 Coliform Agar ES, Chromocult...........................153
Chopped Meat Broth with Vitamin K1...........135 Columbia Agar.....................................................154
Chopped Meat Carbohydrate Medium................135 Columbia Blood Agar..........................................154
Chopped Meat Glucose Agar...............................136 Columbia Blood Agar Base.................................154
Chopped Meat Glucose Broth..............................136 Columbia Blood Agar
Chopped Meat Glucose Medium.........................136 Base with Horse Blood....................................155
Chopped Meat Medium.......................................136 Columbia Blood Agar Base
Chopped Meat Medium with Formate with Horse Blood and Charcoal......................155
and Fumarate...................................................137 Columbia Broth...................................................155
Chopped Meat Medium, Modified......................137 Columbia Broth...................................................156
Chopped Meat Medium, Modified Columbia CNA Agar...........................................156
with Formate and Fumarate.............................138 Columbia CNA Agar, Modified with
Christensen Agar..................................................139 Sheep Blood....................................................157
Christensen Citrate Agar......................................139 Complex Medium................................................157
Christensen Citrate Agar, Modified.....................139 Congo Red Acid Morpholinepropanesulfonic
Christensen Citrate Sulfide Medium....................139 Acid Pigmentation Agar..................................157
Christensen’s Urea Agar......................................140 Congo Red Agar..................................................157
CHROMagar Candida.........................................140 Congo Red Agar..................................................158
CHROMagar E. coli............................................140 Congo Red BHI Agarose Medium......................158
CHROMagar ECC...............................................140 Congo Red BHI Agarose Medium......................159
CHROMagar Listeria..........................................141 Congo Red Magnesium Oxalate Agar.................159
CHROMagar O157..............................................141 Conradi Drigalski Agar........................................160
CHROMagar Orientation.....................................141 Converse Liquid Medium, Levine Modification.160
CHROMagar Salmonella.....................................141 Cooke Rose Bengal Agar.....................................160
CHROMagar Staph. aureus.................................141 Cooked Meat Medium.........................................160
CHROMagar Vibrio.............................................141 Cooked Meat Medium.........................................161
Chromogenic Candida Agar................................142 Cooked Meat Medium with
Chromogenic Listeria Agar.................................142 Glucose, Hemin, and Vitamin K.....................161
Chromogenic MRSA Agar..................................142 Cooked Meat Medium, Modified........................162
Chromogenic Salmonella Esterase Agar.............143 Cooked Meat Medium with Peptone
Chromogenic Substrate Broth..............................143 and Yeast Extract.............................................162
Chromogenic Urinary Tract Cornmeal Agar.....................................................162
Infection (UTI) Medium..................................143 Cornmeal Agar with Strep100 and Tet100..........162
Chromogenic UTI Medium, Clear.......................144 Cornmeal Yeast Glucose Agar.............................163
CIN Agar..............................................................144 CREA...................................................................163
Citrate Medium, Koser’s Modified......................144 Creatinine Agar....................................................163
CLED Agar..........................................................145 Creatinine Medium..............................................163
CLED Agar with Andrade Indicator....................145 CreDm1 Medium.................................................164
Clostridia Medium...............................................145 Crystal Violet Agar..............................................164
Clostridium botulinum Isolation Agar.................145 Crystal Violet Azide Esculin Agar......................165
Clostridium difficile Agar....................................146 Crystal Violet Esculin Agar.................................165
Clostridium Medium............................................147 CT Agar...............................................................165
CTA Agar.............................................................166 Dorset Egg Medium.............................................181
CTA Medium.......................................................166 DTC Agar............................................................181
CTA Medium with Yeast Extract and DTM Agar...........................................................182
Rabbit Serum.................................................166 Dulaney Slants.....................................................182
CTLM Medium....................................................166 Duncan-Strong Sporulation Medium, Modified..182
CVA Medium.......................................................167 Dunkelberg Carbohydrate Medium, Modified....182
Cycloserine Cefoxitin Egg Yolk Fructose Agar..167 Dunkelberg Maintenance Medium......................183
CYE Agar............................................................167 Dunkelberg Semisolid Carbohydrate
CYE Agar, Buffered............................................168 Fermentation Medium...................................183
Cystine Heart Agar..............................................168 E. coli O157:H7 MUG Agar................................183
Cystine Heart Agar with Rabbit Blood................169 Eagle Medium......................................................183
Cystine Tellurite Blood Agar...............................169 Eagle Medium......................................................184
Czapek Dox Agar, Modified................................169 Eagle Medium......................................................185
Czapek Dox Broth...............................................170 Eagle Medium, Modified.....................................185
Czapek Dox Liquid Medium, Modified..............170 Eagle’s Minimal Essential Medium with
Czapek Solution Agar..........................................170 Earle’s Salts and Nonessential Amino Acids..186
Czapek Yeast Extract Agar..................................170 Earle’s Balanced Salts, Phenol Red-Free.............187
DCLS Agar..........................................................171 EB Motility Medium............................................187
Decarboxylase Base, Møller................................171 EC Broth..............................................................187
Decarboxylase Medium Base, Falkow................171 EC Broth with MUG............................................187
Decarboxylase Medium, Ornithine Modified......171 EC Medium, Modified with Novobiocin.............187
Demi-Fraser Broth...............................................172 ECD MUG Agar..................................................188
Deoxycholate Agar..............................................172 Egg Yolk Agar.....................................................188
Deoxycholate Citrate Agar..................................172 Egg Yolk Agar, Modified.....................................188
Deoxycholate Citrate Agar..................................173 Egg Yolk Emulsion..............................................189
Deoxycholate Citrate Agar, Hynes......................173 Egg Yolk Emulsion, 50%.....................................189
Deoxycholate Lactose Agar.................................173 Egg-yolk Tellurite Emulsion 20%.......................189
Dermasel Agar Base............................................174 Eijkman Lactose Medium....................................189
Dermatophyte Test Medium Base........................174 EMB Agar............................................................190
DEV Lactose Peptone MUG Broth.....................174 EMB Agar Base...................................................190
Dextrose Agar......................................................174 EMB Agar, Modified...........................................190
Dextrose Agar......................................................175 Endo Agar............................................................190
Dextrose Ascitic Fluid Semisolid Agar...............175 Endo Agar............................................................191
Dextrose Broth.....................................................175 Endo Agar, LES...................................................191
Dextrose Proteose No. 3 Agar.............................175 Endo Broth...........................................................192
Dextrose Starch Agar...........................................176 Enriched Nutrient Broth......................................192
Diagnostic Sensitivity Test Agar.........................176 Enrichment Broth for Aeromonas hydrophila.....192
Diamonds Medium, Modified..............................177 Entamoeba Medium.............................................192
Diethyl Phosphonate Agar...................................177 Enteric Fermentation Base...................................193
Differential Agar Medium A8 Enterobacter Medium..........................................193
for Ureaplasma urealyticum..........................177 Enterococci Broth, Chromocult...........................193
Dilute Peptone Water...........................................178 Enterococcosel™ Agar........................................194
Dilute Potato Medium..........................................178 Enterococcosel Broth...........................................194
Diphasic Blood Agar Base Medium....................178 Enterococcus Agar...............................................194
Diphasic Blood Agar Medium Enterococcus faecium Medium...........................195
with 10% Blood.............................................178 Erythritol Agar.....................................................195
Diphasic Blood Agar Medium Erythritol Broth....................................................195
with 30% Blood.............................................179 Esculin Agar........................................................195
Diphasic Medium for Amoeba.............................179 Esculin Agar, Modified CDC..............................195
Dixon Agar..........................................................180 Esculin Broth.......................................................195
DNase Agar..........................................................180 Esculin Iron Agar.................................................196
DNase Medium....................................................180 Esculin Mannitol Agar.........................................196
DNase Test Agar..................................................181 ETGPA.................................................................196
DNase Test Agar with Methyl Green...................181 Ethyl Violet Azide Broth.....................................197
DNase Test Agar with Toluidine Blue.................181 Eubacterium Medium..........................................198
Fastidious Anaerobe Agar...................................198 GC Agar with Ampicillin....................................214
Fastidious Anaerobe Agar, Alternative Selective..198 GC Agar with Ampicillin and Gentamicin..........215
Fastidious Anaerobe Agar, Alternative GC Agar with Ampicillin and Tetracycline.........215
Selective with Neomycin, Vancomycin, GC Agar with Chloramphenicol,
and Josamycin...............................................199 Tetracycline, and Ampicillin.........................216
Fastidious Anaerobe Agar, Selective...................199 GC Agar with Defined Supplements...................217
Fastidious Anaerobe Agar, Selective with GC Agar with Penicillin G..................................217
Neomycin and Vancomycin...........................200 GC Agar with Supplement A...............................218
Fay and Barry Medium........................................200 GC Agar with Supplement A
FDA Agar............................................................201 and with VCN Inhibitor.................................219
FDA Broth...........................................................201 GC Agar with Supplement A and
Fecal Coliform Agar, Modified...........................201 with VCTN Inhibitor.....................................219
Fermentation Broth..............................................201 GC Agar with Supplement B...............................220
Fermentation Medium..........................................202 GC Medium, New York City Formulation..........221
F-G Agar..............................................................202 GCII Agar............................................................221
F-G Agar with Selenium......................................202 GCII Agar............................................................222
F-G Broth.............................................................203 GC-Lect™ Agar..................................................222
FGTC Agar...........................................................203 GC Medium with Chloramphenicol....................223
Fildes Enrichment Agar.......................................204 GCA Agar with Thiamine...................................223
Fish Peptone Agar................................................204 Gelatin Agar.........................................................224
Fish Peptone Broth...............................................204 Gelatin Infusion Broth.........................................224
Five g Agar..........................................................205 Gelatin Medium...................................................225
Fletcher Medium..................................................205 Gelatin Metronidazole Cadmium Medium..........225
Fletcher Medium with Fluorouracil.....................205 Gelatin Phosphate Salt Agar................................225
Fletcher’s Semisolid Medium..............................205 Gelatin Phosphate Salt Broth...............................226
FLN Medium.......................................................205 Gelatin Salt Agar.................................................226
Fluid Sabouraud Medium....................................206 Gelatinase Test Medium......................................226
Fluid Thioglycolate Agar.....................................206 Gluconate Peptone Broth.....................................226
Fluid Thioglycolate Agar with Glucose Nutrient Agar.........................................226
Calcium Carbonate........................................206 Glucose Phosphate Broth.....................................227
Fluid Thioglycolate Medium...............................206 Glucose Salt Teepol Broth...................................227
Fluid Thioglycolate Medium Glucose Salts Medium.........................................227
with Beef Extract...........................................207 Glutarate Medium................................................227
Fluid Thioglycolate Medium Glycerol Agar......................................................228
without Glucose or E Indicator....................207 Glycerol Asparagine Meat Agar..........................228
h
Fluid Thioglycolate Medium GN Broth, Hajna..................................................228
with K Agar...................................................207 Gonococcus Medium...........................................229
Fluid Thioglycolate Medium GP Agar...............................................................229
with Rabbit Serum.........................................207 GPHF Agar..........................................................229
Fluorocult E. coli 0157:H7 Agar.........................208 Granada Medium.................................................229
FN Medium..........................................................208 Group A Selective Strep Agar
Folic Acid Agar...................................................208 with Sheep Blood..........................................230
Formate Fumarate Medium.................................209 H Agar.................................................................230
FRAG Agar..........................................................209 H Broth................................................................231
Francisella tularensis Isolation Medium.............210 H Diphasic Medium.............................................232
Freezing Medium.................................................210 Haemophilus ducreyi Medium.............................233
G Medium............................................................210 Haemophilus ducreyi Medium, Revised..............233
GAM Agar...........................................................211 Haemophilus influenzae Defined Medium MI....234
GAM Semisolid...................................................211 Haemophilus influenzae Defined Medium
Gardnerella vaginalis Selective Medium............211 MI-Cit............................................................234
GBNA Medium....................................................211 Haemophilus Medium.........................................235
GBS Agar Base, Islam.........................................212 Haemophilus Test Medium..................................235
GBS Medium, Rapid............................................212 Ham’s F-10 Medium............................................236
GC Agar...............................................................212 HBT Bilayer Medium..........................................236
GC Agar...............................................................213 Heart Infusion Agar.............................................237
Heart Infusion Agar with Glucose.......................238 Histoplasma capsulatum Broth............................253
Heart Infusion Agar with 0.1% Glucose..............238 HL Agar...............................................................254
Heart Infusion Agar (pH 7.6) HO-LE Trace Elements Solution.........................254
with Inactivated Horse Serum.......................238 Horse Blood Agar................................................254
Heart Infusion Agar with Inactivated Horse Serum Agar...............................................255
Horse Serum..................................................238 Horse Serum Broth..............................................255
Heart Infusion Agar with Inactivated Hoyle Medium.....................................................255
Horse Serum, NaCl, and Penicillin...............238 Hoyle Medium Base............................................255
Heart Infusion Agar with Rabbit Blood...............239 HR Antifungal Assay Medium
Heart Infusion Broth............................................239 Buffered with MOPS.....................................256
Heart Infusion Broth (pH 7.5) with Hugh-Leifson’s Glucose Broth............................256
Inactivated Human Serum and Hydroxybenzoate Agar........................................256
Yeast Extract..................................................239 Hydroxybenzoate Broth.......................................257
Heart Infusion Broth Imidazole Utilization Medium.............................257
with Additives for Staphylococcus................239 Indole Medium.....................................................258
Heart Infusion Broth Indole Nitrite Medium.........................................258
with Additives for Streptobacillus.................239 Infection Medium................................................258
Heart Infusion Broth with Inactivated Infusion Broth......................................................258
Horse Serum and Fresh Yeast Extract...........240 Inhibitory Mold Agar...........................................259
Heart Infusion Broth with Inactivated Inositol Brilliant Green Bile Salts Agar...............259
Horse Serum, Fresh Yeast Extract, Inositol Urea Caffeic Acid Medium....................259
and Sucrose....................................................240 Intracellular Growth Phase Medium....................260
Heart Infusion Medium Ion Agar for Ureaplasma.....................................260
with Fetal Bovine Serum...............................240 Irgasan Ticarcillin Chlorate Broth.......................261
Heart Infusion Tyrosine Agar..............................240 Iron Agar, Lyngby................................................261
Hektoen Enteric Agar..........................................241 Iron Milk Medium...............................................261
Helicobacter pylori Isolation Agar......................241 Iso-Sensitest Agar................................................261
Helicobacter pylori Selective Medium................241 Iso-Sensitest Broth...............................................262
Hemo ID Quad Plate with Growth Factors..........242 Isoleucine Hydroxamate Medium........................262
Hemorrhagic Coli Agar.......................................242 Jones-Kendrick Pertussis Transport Medium......262
Herellea Agar.......................................................242 Jordan’s Tartrate Agar..........................................263
Hershey’s Tris-Buffered Salts Medium...............243 Kanamycin Vancomycin Blood Agar..................263
Hexamita Medium...............................................243 Kanamycin Vancomycin Laked Blood Agar.......263
HiCrome™ Aureus Agar Base............................244 Kasai Medium......................................................264
HiCrome Coliform Agar......................................244 KCN Broth...........................................................264
HiCrome Coliform Agar with Novobiocin..........245 Keister's Modified TYI-S-33 Medium................264
HiCrome E. coli Agar A......................................245 Kelly Medium, Nonselective Modified...............264
HiCrome E. coli Agar B......................................245 Kelly Medium, Selective Modified.....................265
HiCrome ECC Agar.............................................245 KF Streptococcus Agar........................................266
HiCrome ECC Selective Agar.............................246 KF Streptococcus Agar........................................267
HiCrome Enterococci Broth................................247 KF Streptococcus Broth.......................................267
HiCrome Listeria Agar Base, Modified..............247 Kirchner’s Enrichment Medium..........................267
HiCrome MacConkey-Sorbitol Agar...................247 K-L Virulence Agar.............................................268
HiCrome MacConkey-Sorbitol Agar Klebsiella Medium...............................................269
with Tellurite-Cefixime Supplement.............248 Klebsiella Selective Agar....................................269
HiCrome M-CP Agar Base..................................248 Kligler Iron Agar.................................................270
HiCrome MS.O157 Agar.....................................249 Knisely Medium for Bacillus anthracis..............270
HiCrome MS.O157 Agar with Tellurite..............249 Koch’s K1 Medium..............................................270
HiCrome Rapid Coliform Broth..........................249 Korthof Medium..................................................271
HiCrome Rapid Enterococci Agar.......................250 Korthof Medium, Modified.................................271
HiCrome Salmonella Agar...................................250 Koser Citrate Medium.........................................271
HiCrome Salmonella Chromogen Agar...............250 Kosmachev’s Medium.........................................271
HiCrome UTI Agar, Modified.............................250 Kupferberg Trichomonas Base............................271
Histoplasma capsulatum Agar.............................251 Kupferberg Trichomonas Broth...........................272
Histoplasma capsulatum Agar.............................252 L Agar..................................................................272