Guide to Micro and Nano Fluidics - Harvard Apparatus

42 Pages·2009·2.97 MB·English

Guide to
Microfluidic
& Nanofluidic
Solutions
pumps, heaters,
chips & connectors
www.harvardapparatus.com
Table of Contents
Micro/Nanofluidics Introduction
Why Miniaturization?................................................................................................3
Evolution of Research Reactor Fluidic Devices............................................................4-5
What do we mean by Micro and Nanofluidics? ..........................................................6-7
Applications of Microfluidics ......................................................................................8
Applications of Nanofluidics ......................................................................................9
Let Our Expertise Work for You
108 years of Experience in Low-Flow Science ............................................................10
Harvard Apparatus Micro and Nanofluidics Advantage ................................................11
Harvard Apparatus Family of Companies ..................................................................12
Pump Application Questionnaire ..............................................................................13
Syringe Pumps
Nanomite ............................................................................................................14
Pump 11 Pico Plus................................................................................................14
Pump 22..............................................................................................................14
Pump 33 (Dual Syringe) ........................................................................................14
Microliter Syringe Pump Module ..............................................................................14
NEW PHD ULTRA™................................................................................................15
PHD 22/2000 Programmable................................................................................15
PHD 22/2000 with Microliter Syringe Rack..............................................................15
PHD 4400 Hpsi ..................................................................................................15
Multiple Animal Feeding System ..............................................................................15
s NEW PHD ULTRA™ ..............................................................................................16-17
t
c Pico Plus Syringe Pump..............................................................................................18
u
d Model 33 Syringe Pump ............................................................................................19
o
r Nanoleader Series of Pumps
P
c NLS110P Pressure Pump..................................................................................20-21
di PLI-100 Nano Dispenser ......................................................................................22-23
ui
fl General Accessories
o
n Microfluidic Chip Holders and Connection Kits
a
N Fluidic Connect 4515........................................................................................24
& Fused Silica Connection Kit ................................................................................24
c Teflon Connection Kit ........................................................................................24
di Stainless Steel Connection Kit ............................................................................24
ui
fl Microtiter Plate MPG 96.3................................................................................24
o
r Microfluidic Chips
c
Mi Microreactor Chips ..........................................................................................25
Cross Channel Chips ........................................................................................25
o
t Micromixer Chips..............................................................................................25
e
d Temperature Control
ui WP-10 and WP-16 Warmed Platforms ..............................................................26
G
TB-3 CS Thermal Insert ....................................................................................26
|
s CO Microscope Stage Incubator ........................................................................26
u
at SH2-27B and SF-28 Single Inline Solution Heaters ..................................................27
r
a Perfusion Valve Control Systems ........................................................................27
p
p Specialized Fluidic Products ..................................................................................28-29
A
Applications
d
ar Reactor/Detector ................................................................................................30
v
r Reactor/Microspheres ..........................................................................................31
a
H Device ............................................................................................................32-34
Detection ............................................................................................................34
Reactor................................................................................................................35
Bibliography ........................................................................................................36-39
Getting to know Harvard Apparatus............................................................................40
Technical Service ......................................................................................................41
2 phone [phone] • email [email protected] • web www.harvardapparatus.com
Micro/Nanofluidics Introduction
Why Miniaturization?
Volume of 1µl 1nl 1pl
Is a Cube of 1mm3 100µm3 10µm3
# of Molecules 600,000,000 600,000 600
(1nM solution)
Diffusion Time 17 min 10 s 100 ms
# of Reactions 1.5/min/cm2 250/s/cm2 2,500,000/s/cm2
(diffusion controlled)
Source:A.Manz,ISASDortmun
• 10 Fold Miniaturization • 1000 x smaller volume
• 100 x faster separation • 10 x lower reagent
H
a
• 100 x capillarity consumption rv
a
r
d
A
p
The two charts above illustrate why there is so much interest in p
a
r
Micro and Nanofluidics: a
t
u
s
|
Reduced Sample Size: Researchers can use expensive or precious G
u
reagents in small quantity, reducing expense and allowing for id
e
increased research on trace amounts of sample.
t
o
M
Decreased Scale of Experimentation: Experiments ic
r
are easier to automate; easier to do simultaneous of
lu
multiple experiments in the same physical space as a id
ic
macro experiment.
&
N
a
Increased Experimental Productivity: The ability n
o
to automate multiple simultaneous complete experi- f
lu
ments creates increased experimental productivity. id
ic
P
r
o
Conclusions: d
u
c
• Study more variables simultaneously per time period t
s
• Study smaller phenomenon for greater
understanding
• Increased experimental productivity
• More information faster than conventional
techniques
phone [phone] • email [email protected] • web www.harvardapparatus.com 3
Micro/Nanofluidics Introduction
Evolution of Research Reactor
Both Batch and Continuous Flow
n
i
m ll
a
/ m
l S
p
s
t
c
u
d
o
r
P
dic e e Technicon
flui at Siz Continuous
o R
n e
a l Flow
N w p
m
& o
a
c Fl S
di
ui
fl
o
r
c
Mi
o
t
e
d
ui
G
|
s
u
t
a
r
a
p
p
A n
d mi e
r
a g
v / r
ar l a
H m L
1950 1960 1970 1980
Years
4 phone [phone] • email [email protected] • web www.harvardapparatus.com
Micro/Nanofluidics Introduction
Fluidic Devices
Lab-on-a-chip
Sensors Tissues Reactors
Flow Injection H
a
Analysis Continuous Flow rv
a
r
d
A
p
p
a
r
a
t
u
s
|
G
u
id
e
Batch Reactors t
o
M
ic
r
o
f
lu
id
ic
&
N
a
n
o
f
lu
id
Glassware Reactors ic
P
r
o
d
u
c
t
s
1990 2000 2010 AND BEYOND!
Years
phone [phone] • email [email protected] • web www.harvardapparatus.com 5
Micro/Nanofluidics Introduction
What do we mean by Micro and Nanofluidics?
The science of micro and nanofluidics is the building of miniaturized devices with chambers
and tunnels for the containment and flow of fluids. With devices measured at the micrometer
level and fluids measured in nanoliters and picoliters, microfluidic and nanofluidic devices are
widely used in the applications shown in this guide (pgs 8 & 9).
e
nt
e
w
T
of
y
sit
er
v
ni
U
e
h
t
of
er
y
e
h
hl
o
K
h
c
etri
Di
y
s b
ct oto
u Ph
d
o
r
P
c
di
ui
fl
o
n
a
N
&
c
di
ui
fl
o
r
c
Mi
o
t
e
d
ui
G
|
s
u
t
a
r
a
p
p
A
d
r
a
v
r
a
H
6 phone [phone] • email [email protected] • web www.harvardapparatus.com
Micro/Nanofluidics Introduction
Microfluidics: Nanotechnology provides:
(10-6) length scale is on the order of a micron. • Exquisite new tools to engineer novel
Liquid micro systems and components differ materials and devices at the nanoscale level,
H
a
from macro systems primarily due to device A nanometer, one billionth of a meter, is r
v
a
fabrication of miniature systems. about 10,000 times narrower than a r
d
human hair A
p
Nanofluidics: p
a
• Biological systems on the nano scale resem- r
(10-9) The science of building microminiaturized a
ble the semiconductor world where a mole- tu
devices with chambers and tunnels for the s
cule or a biological system is a series of |
containment and flow of fluids measured at the G
atoms and energies u
nanometer level. Harvard Apparatus 108 years id
e
of experience in low-flow science provides you • Monitoring temperature, pressure, t
o
with: low flow (microliter, nanoliter, picoliter and inserting things into a cell or moving fluids M
ic
femtoliter) fluid delivery systems, with high becomes an exercise in moving atoms and r
o
f
accuracy and precision, pulse free pumping maintaining or monitoring energies lu
id
mechanisms and critical accessories for micro ic
and nano fluidics. &
N
a
n
o
f
lu
id
ic
P
r
o
d
u
c
t
s
phone [phone] • email [email protected] • web www.harvardapparatus.com 7
Micro/Nanofluidics Introduction
Applications of
Microfluidics
BIOCHEMISTRY
• Enzymatic assays
• The Polymerase chain reaction
• Nucleic acid arrays
• Biomolecular separations
• Immunohybridization reactions
• Piercing structures for DNA injection
MICROFLUIDICS
s
t
c
u
d
o CELL BIOLOGY
r
P
• Flow cytometry/sorting
c
di • Sperm/embryo tools: sperm motility, in vitro
ui
fl fertilization, embryo branding
o
n
a • Force measurements with bending cantilevers
N
• Dialectrophoresis/electroporation
&
c • Impedance monitoring for cell motility
di
ui and micromotion
ofl • Chemical/physical
r
c substrate patterning
Mi
o
t
e
d
ui
G
|
s
u
t
a
r
a
p
p
A
d
r
a
v
r
a
H
8 phone [phone] • email [email protected] • web www.harvardapparatus.com
Micro/Nanofluidics Introduction
Applications of
Nanofluidics
BIOLOGY
Physiology
(filtration, superhydrophobicity, biolubrication,
ion channels, actin/myosin, aquaporins)
Genetics Nanobioscience
(DNA) (single molecule
studies)
Biochemistry Biophysics
CHEMISTRY PHYSICS
H
Colloid Physics of Fluids a
r
Chemistry (CFD, cavitation, slip flow) va
r
(DLVO theory, electrokinetics, d
A
Donnan equil,. semipermeability) Thermodynamics p
p
a
r
a
t
u
NANOFLUIDICS s
|
G
u
Polymer Science Surface Science ide
(DNA, protein conformation) (SFA, wetting, absorption) to
M
Separation Science Tribology icr
o
(chromatographic (brush layers) flu
packings) id
ic
&
N
Membrane Science a
Soil Science n
(nanoporosity, size exclusion, o
(water potential, zeolites) flu
semipermeability) id
ic
P
ENGINEERING r
o
d
Microengineering u
c
t
(stiction, device fabrication) s
Bioengineering
(tissue engineering, cartilage)
phone [phone] • email [email protected] • web www.harvardapparatus.com 9
Let Our Expertise Work for You
108 Years of Experience
in Low Flow Science
Harvard Apparatus offers superior
solutions for the challenges of
microfluidic and nanofluidic
applications. Harvard Apparatus’
108 years of experience in low-flow
science provides you low flow
(microliter, nanoliter, picoliter and
femtoliter) fluid delivery systems
with high accuracy and precision,
pulse free pumping mechanisms
s and critical accessories for micro-
t
c
u
d fluidics and nanofluidics.
o
r
P
c Harvard Apparatus offers single-
di
ui and multi-channel volume and
fl
o
n
a pressure controlled pumps with flow
N
& rates from picoliters to milliliters.
c
di We can provide standard models,
ui
fl
o specials and OEM modules designed
r
c
Mi to meet your application needs.
o
t
In addition to pumps we carry
e
d
ui heaters/coolers (syringe, in-line
G
|
s and chip), syringes, microfluidic
u
t
a
r chips, connectors and tubing.
a
p
p
A
d
r
a
v
r
a
H
technical support staff
Our experienced
READY any time!
is to assist you at
10 phone [phone] • email [email protected] • web www.harvardapparatus.com