GROWTH, DEVELOPMENT AND POST HARVEST BEHAVIOUR IN FRUITS OF ACID LIME (Citrus aurantifolia Swingle) AS INFLUENCED BY PRE-HARVEST SPRAYS OF CERTAIN MINERALS AND GROWTH REGULATORS By ANOOP MATHEW ABRAHAM, B.Sc., (Ag.) THESIS SUBMITTED TO THE ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN AGRICULTURE (HORTICULTURE) DEPARTMENT OF HORTICULTURE SRI VENKATESWARA AGRICULTURAL COLLEGE ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY TIRUPATI - 517 502 (A.P) SEPTEMBER, 2004 CERTIFICATE This is to certify that Mr. ANOOP MATHEW ABRAHAM, has satisfactorily prosecuted the course of research and that the thesis entitled “GROWTH, DEVELOPMENT AND POST HARVEST BEHAVIOUR IN FRUITS OF ACID LIME (Citrus aurantifolia Swingle) AS INFLUENCED BY PRE-HARVEST SPRAYS OF CERTAIN MINERALS AND GROWTH REGULATORS" submitted is the result of original research work and is of sufficiently high standard to warrant its presentation to the examination. I also certify that the thesis or part there of has not been previously submitted by him for a degree of any University. Date : (Dr. K. HARI BABU) Major Advisor Place : CERTIFICATE This is to certify that the thesis entitled “GROWTH, DEVELOPMENT AND POST HARVEST BEHAVIOUR IN FRUITS OF ACID LIME (Citrus aurantifolia Swingle) AS INFLUENCED BY PRE-HARVEST SPRAYS OF CERTAIN MINERALS AND GROWTH REGULATORS" submitted in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN AGRICULTURE of the Acharya N.G. Ranga Agricultural University, Hyderabad, is a record of the bonafide research work carried out by Mr.ANOOP MATHEW ABRAHAM under our guidance and supervision. The subject of the thesis has been approved by the Student's Advisory Committee. No part of the thesis has been submitted by the student for any other degree or diploma. The published part has been fully acknowledged. All assistance and help received during the course of the investigations have been duly acknowledged by the author of the thesis. (Dr. K. HARI BABU) Chairman of the Advisory Committee Thesis is approved by the Student Advisory Committee Chairman (Dr. K. HARI BABU) _______________ Prinicipal Scientist (Hort.) AICRP on Tropical Fruits (Citrus) S.V.Agricultural College Campus Tirupati - 517 502 Member (Dr. R. SRI HARI BABU) _______________ Professor & Head Department of Horticulture S.V.Agricultural College Tirupati - 517 502 Member (Dr. N. RAMAVATHARAM ) ______________ Professor & Head Department of Soil Science & Agricultural Chemistry S.V.Agricultural College Tirupati - 517 502 CONTENTS Chapter Title Page No. I INTRODUCTION II REVIEW OF LITERATURE III MATERIALS AND METHODS IV RESULTS V DISCUSSION VI SUMMARY LITERATURE CITED LIST OF ILLUSTRATIONS Fig. Page No. Title No. 1 Influence of pre-harvest sprays of minerals and growth regulators on fruit length (cm) of acid lime 2 Influence of pre-harvest sprays of minerals and growth regulators of fruit diameter (cm) of acid lime 3 Influence of pre-harvest sprays of minerals and growth regulators on fruit weight (g) of acid lime 4 Influence of pre-harvest sprays of minerals and growth regulators on rind thickness (mm) of acid lime 5 Influence of pre-harvest sprays of minerals and growth regulators on rag (%) of acid lime 6 Influence of pre-harvest sprays of minerals and growth regulators on juice content % of acid lime 7 Influence of pre-harvest sprays of minerals and growth regulators on physiological loss of weight (%) of acid lime 8 Influence of pre-harvest sprays of minerals and growth regulators on total titratable acidity (%)of acid lime 9 Influence of pre-harvest sprays of minerals and growth regulators on total soluble solids of acid lime 10 Influence of pre-harvest sprays of minerals and growth regulators on brix acid ratio of acid lime 11 Influence of pre-harvest sprays of minerals and growth regulators on ascorbic acid content (mg / 100 g) of acid lime 12 Influence of pre-harvest sprays of minerals and growth regulators on fruit softness (sensory score) of acid lime 13 Influence of pre-harvest sprays of minerals and growth regulators on peel colour (visual score) of fruits of acid lime 14 Influence of pre-harvest sprays of minerals and growth regulators on shelf life of acid lime LIST OF TABLES S Page No. Title No. Standard Week Wise Meteorological Data During The Crop 3.1 Growth Period (January 2004 - July 2004) Influence of pre-harvest sprays of minerals and growth 1 regulators on fruit length (cm) of acid lime Influence of pre-harvest sprays of minerals and growth 2 regulators of fruit diameter (cm) of acid lime Influence of pre-harvest sprays of minerals and growth 3 regulators on fruit weight (g) of acid lime Influence of pre-harvest sprays of minerals and growth 4 regulators on rind thickness (mm) of acid lime Influence of pre-harvest sprays of minerals and growth 5 regulators on rag (%) of acid lime Influence of pre-harvest sprays of minerals and growth 6 regulators on juice content % of acid lime Influence of pre-harvest sprays of minerals and growth 7 regulators on seed number of acid lime Influence of pre-harvest sprays of minerals and growth 8 regulators on seed weight of acid lime Influence of pre-harvest sprays of minerals and growth 9 regulators on physiological loss of weight (%) of acid lime Influence of pre-harvest sprays of minerals and growth 10 regulators on total titratable acidity (%)of acid lime Influence of pre-harvest sprays of minerals and growth 11 regulators on total soluble solids of acid lime Influence of pre-harvest sprays of minerals and growth 12 regulators on brix acid ratio of acid lime Influence of pre-harvest sprays of minerals and growth 13 regulators on ascorbic acid content (mg / 100 g) of acid lime Influence of pre-harvest sprays of minerals and growth 14 regulators on fruit softness (sensory score) of acid lime Influence of pre-harvest sprays of minerals and growth 15 regulators on peel colour (visual score) of fruits of acid lime Influence of pre-harvest sprays of minerals and growth 16 regulators on shelf life of acid lime LIST OF SYMBOLS AND ABBREVIATIONS % - Percentage @ - At the rate of °C - degree celsius B - Boron CaCl - Calcium chloride 2 CD - Critical difference cm - centimetre DAFS - Days after fruit set DAH - Days after harvest et al., - and others Fig. - Figure g - gram GA - Gibberellic acid 3 i.e., - That is l - litre mg - Milli gram ml - millilitre mm - millimeter mt - Million tonnes MH - Maleic hydrazide N - Normal NAA - Naphthalene acetic acid no. - Number NS - Non-significant ppm - parts per million RBD - Randomized block design S - Significant SD - Standard deviation SEM - Standard error of mean viz. - Namely L-1 - per litre ai - active ingredient ACKNOWLEDGEMENTS It is by unfathomable grace and blessings of Almighty that I have been able to bring into light this humble piece of work for which I am extremely indebted. I have immense pleasure in expressing my earnest gratitude to my major advisor Dr. K.Hari Babu, Principal Scientist, AICRP on Tropical Fruits (Citrus), Tirupati for his perspicacity, learned counsel, and meticulous guidance at every stage in the presentation and editing of the thesis. I am greatly indebted to Dr. R. Sri Hari Babu, Professor & Head, Department of Horticulture, S.V. Agricultural College, Tirupati, member of my advisory committee as the indefatigability of his astute brain has helped a long way in consummation of this laconic tome. I deem it my privilege to extol my profound etiquette and sincere feelings of gratitude to Dr. K. Ramavataram, Professor & Head, Department of Soil Science and Agricultural Chemistry, S.V.Agricultural College, Tirupati, member of my advisory committee. It is with great pleasure that I take this opportunity to express my sincere gratitude to Dr. E. Nagabhushanam Reddy, Professor, Sri T. Dayanand, Associate Professor, Sri L. Naram Naidu, Sri K.M. Yuvaraj and Smt. Madhavi, Assistant Professors, Department of Horticulture, S.V.Agricultural College, Tirupati and also to the staff of AICRP on Tropical Fruits (Citrus), Tirupati, for the help rendered during my study. Diction is not enough to extend my unboundful gratitude to my beloved parents Mr. M.M. Abraham, and Ms. Susan Abraham brother Ashok Abraham, for their affection and encouragement throughout my study and for their sacrifices made for my upbringing. I wish to express my thanks to my dearest friends Manjunath, Sudha, and Yuvaraj. I am very grateful to my seniors Usha Rani, Srilatha and Umamaheswari. I avail this opportunity to express my deep feelings to my beloved colleagues Sashidar and Hanuman Naik and junior friends Kiranmayi, Sailaja, Sandhya, Sampath and Biju. I wish to express my thanks to my friends Kiran Kumar, Chaitanya Varma, Mahendra Dia, Gnanesh, Sampath and Dasaradh and others for their co-operation extended during my stay here. I am thankful to the authorities of ICAR for the allotment of PG seat at S.V. Agricultural College, Tirupati. I also thankful to Acharya N.G. Ranga Agricultural University, Hyderabad for the facilities provided during my study. My heartful thanks to K. Visweswara rao, Department of Arts and Photography and Mr. Hemanth, Hemanth Computer Centre for their excellent work, patiently carried out in getting thesis its present shape. (Anoop Mathew Abraham … ) DECLARATION I, Mr. ANOOP MATHEW ABRAHAM, here by declare that the thesis entitled “GROWTH, DEVELOPMENT AND POST HARVEST BEHAVIOUR IN FRUITS OF ACID LIME (Citrus aurantifolia Swingle) AS INFLUENCED BY PRE-HARVEST SPRAYS OF CERTAIN MINERALS AND GROWTH REGULATORS" submitted to Acharya N.G. Ranga Agricultural University, Hyderabad for the degree of Master of Science in Agriculture is the result of original research work done by me. I also declare that the material contained in this thesis has not been published earlier. Date : (ANOOP MATHEW ABRAHAM)
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