Green Fluorescent Protein

534 Pages·1999·15.064 MB·English

by  P. Michael Conn (Eds.)

Contributors to Volume 302
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
MICHAEL T. AYDERSON (26), Department of North Carolina--Chapel Hill, Chapel ,lliH
Pathology, University of Alabama ta Bir- North aniloraC 99572
mingham, Birmingham, Alabama 49253 ROBERT KANEVRE~rC (16, 17), Department of
TAKASHI AOKI (23), Department of -mehcoiB ygoloiborciM dna Immunology, ehT retneC
,yrtsi Faculty of Pharmaceutical ,secneicS for ecnellecxE ni recnaC ,hcraeseR and ehT
Health Sciences University of Hokkaido, retneC for ecnellecxE ni Arthritis dna Rheu-
-irakihsI Tobetsu, Hokkaido ,3920-160 ,ygolotatn Louisiana State ytisrevinU -ideM
napaJ lac ,retneC Shreveport, Louisiana 03117
HOWARD J. ATKINSON (27), Centre for Plant SvEermN R. CRONIN (8), Department of Biol-
,secneicS University of Leeds, Leeds LS2 ogy, University of ,ainrofilaC naS ,ogeiD
,TJ9 United Kingdom La Jolla, California 7430-39029
Joseph C. AYooB (15), Department of lleC G~ttssou A. DABIRI (15), Department of lleC
and Developmental Biology, ytisrevinU of dna Developmental Biology, ytisrevinU of
ainavlysnneP School of ,enicideM -ledalihP ainavlysnneP School of ,enicideM -ledalihP
phia, Pennsylvania 8506-40191 phia, Pennsylvania 8506-40191
GEORGE RANTING (1), Department of -GiB TRISHA N. DAVIS (10), Department of Bio-
,yrtsimehc University of Bristol School of chemistry, University of Washington,
Medical Sciences, Bristol BS8 I ,DT ,elttaeS Washington 59189
detinU Kingdom TOMMY DUO(cid:127)G (4), CLONTECH Labora-
LARRY S. BARAK (14), Howard Hughes -ideM ,seirot Inc., Palo Alto, California -30349
lac Institute dna Department of Cell Biol- 0324
,ygo Duke ,ytisrevinU Durham, North -raC Yu FAN~ (18), CLONTECH ,seirotarobaL
olina 01772 Inc., Palo Alto, California 0324-30349
K. K. BENCE (19), Brown ,ytisrevinU Provi- STEPHEN S. G. FERGUSON (14), John .P Ro-
,ecned Rhode Island 21920 strab hcraeseR Institute, London, ,oiratnO
LuIZ E. BERMUDEZ (25), Kuzell Institute for adanaC N6A 5K8
Arthritis and Infectious ,sesaesiD ainrofilaC MARK R. FLORY (10), Molecular and ralulleC
cificaP Medical ,retneC naS ,ocsicnarF -ilaC Biology Program, University of -gnihsaW
fornia 51149 ton, ,elttaeS Washington 59189
L. A. C. BLAre (19), Brown ,ytisrevinU Provi- TEllCH1FoRtJlCm (20), Department of -celoM
,ecned Rhode Island 21920 ralu Neurobiology, ehT Institute of lacideM
~rEVINNEJ K, MOSMULB (27), ertneC for Plant ,ecneicS ehT University of Tokyo, Shiro-
,secneicS University of Leeds, Leeds LS2 kanedai, Minato-ku, Tokyo ,9368-801
,TJ9 United Kingdom napaJ
MARC G. CARON (14), Howard Hughes -ideM DAVID W. GALBRAITH (26), Department of
lac Institute and Department of Cell Biol- Plant ,secneicS ytisrevinU of Arizona, -cuT
,ygo Duke ,ytisrevinU Durham, North -raC son, Arizona 12758
olina 01772 V1RGINIE GEORGET (12), Montpellier School
WAYNE E. CASCIO (29), Department of -ideM of ,enicideM INSERM ,934-U 09043 Mont-
,enic School of Medicine, University of ,reillep ecnarF
ix
X CONTRIBUTORS TO VOLUME 302
HANs-HERMANN GERDES (2), Department of YUKIO KIMATA (31), hcraeseR and Education
Neurobiology, University of ,grebledieH retneC for Genetic Information, Nara -itsnI
02196-D ,grebledieH Germany tute of Science and Technology, Ikoma,
GONTHER GERISCH (7), Max-Planck-lnstitut Nara ,10-036 Japan
for Biochemie, D-82152 Martinsried, AD~t~L A. KING (33), School of lacigoloiB and
Germany Molecular ,secneicS Oxford Brookes -inU
GORDON L. HAGER (9), Laboratory of -peceR ,ytisrev Oxford 0)30BP, United Kingdom
tor Biology and Gene Expression, National
KATHERINE S. Koch (23), Department of
Cancer Institute, National Institutes of
,ygolocamrahP School of ,enicideM -revinU
,htlaeH Bethesda, Maryland 5505-29802
sity of ,ainrofilaC San Diego, La ,alloJ -ilaC
RANDOLPH Y. HAMPTON (8), Department of fornia 6360-39029
Biology, University of California, naS
,ogeiD La Jolla, California 7430-39029 KENn KOHNO (31), Research and Education
retneC for Genetic Information, Nara Insti-
FRANK HANAKAM (7), Micromet GmbH,
tute of Science and Technology, Ikoma,
25128-D ,deirsnitraM Germany
Nara 630-0l, Japan
ROGER HEIM (34), Aurora Biosciences Cor-
poration, naS Diego, California 12129 SABINE KUPZlG (1), Department of Biochem-
,yrtsi University of Bristol School of Med-
JOHN C. HERR (24), Department of Cell Biol-
ical Sciences, Bristol BS8 1TD, United
ogy, University of ,ainigriV ,ellivsettolrahC
Kingdom
ainigriV 80922
LEONARD A. HERZENBERO (26), Department ENAHPETS A. LAPORTE (14), Howard Hughes
of Genetics, Stanford University Medical Medical Institute and Department of Cell
School, Stanford, California 8135-50349 Biology, Duke ,ytisrevinU Durham, North
ROBERT M. HOFPMAN (3), An#Cancer, Inc., aniloraC 01772
naS Diego, California 11129 SAN SAN LEE (1), Department of -simehcoiB
CHIAO-CH1AN HUANG (4, 18, 36), CLON- ,yrt ytisrevinU of Bristol School of lacideM
TECH ,seirotarobaL Inc., Palo Alto, -ilaC ,secneicS Bristol BS81TD, detinU Kingdom
fornia 0324-30349
HYAM L. LEF:ERT (23), Department of -rahP
SATOSHI INOUYE (37), Scripps Institution of macology and retneC for Molecular -teneG
Oceanography, University of ,ainrofilaC ,sci School of ,enicideM ytisrevinU of -ilaC
naS Diego, La Jolla, ainrofilaC 2020-39029 fornia, naS Diego, La Jolla, California
XIN JIANG (36), CLONTECH ,seirotarobaL 6360-39029
Inc., Palo Alto, California 0324-30349
JOHN J. SRETSAMEL (29), Department of lleC
HPOTSIRHC KAETHER (2), Department .fo Biology and Anatomy, School of ,enicideM
Neurobiology, University of ,grebledieH ytisrevinU of North Carolina--Chapel ,lliH
02196-D ,grebledieH Germany
Chapel Hill, North Carolina 99572
NEVETS R. KAIN (4, 5, 18, 28, 30, 36), CLON-
NHOJ P. LEvY (28, 30, 35), Molecular Oncol-
TECH ,seirotarobaL Inc., Palo Alto, -ilaC
ygo Laboratory, Human Gene Therapy -eR
fornia 0324-30349
hcraes Institute, Iowa Health System, Des
MARKO KALLIO (24), Department of Cell Bi-
,senioM Iowa 2023-90305
ology, University of Virginia, -settolrahC
,elliv Virginia 80922 XIANQIANG Ll (4, 18, 36), CLONTECH Lab-
,seirotaro Inc., Palo Alto, Cahfornia
MOTOYA KArSUKI (21), Department of DNA
0324-30349
Biology and Embryo Engineering, -eR
hcraes retneC of Animal Models for Human CHUN REN LIM (31), hcraeseR and noitacudE
,sesaesiD ehT Institute of Medical ,ecneicS retneC for Genetic Information, Nara Insti-
ehT University of Tokyo, Shirokanedai, tute of Science dna Technology, Ikoma,
Minato-ku, Tokyo ,9368-801 Japan Nara ,10-036 Japan
CONTRIBUTORS TO VOLUME 302 xi
CHARLES J. LINk, JR. (28, 30. 35), raluceloM KENJI NAKAMURA (21), Department of DNA
Oncology Laboratory, Human Gene -rehT Biology dna Embryo Engineering, -eR
apy hcraeseR ,etutitsnI Iowa Health ,metsyS hcraes retneC of Animal Models for Human
seD Moines, Iowa 2023-90305 ,sesaesiD ehT Institute of Medical ,ecneicS
+VI~NOL LY~ARC;ER (16, 17), Department of ehT University of Tokyo, ,iadenakorihS
ygoloiborciM and hnmunology, Louisiana Minato-ku, Tokyo ,9368-801 Japan
State University Medical Center, Shreve- KAZUKt NAKAO (21), Department of DNA -iB
port, Louisiana 03117 ology and Embryo ,gnireenignE hcraeseR
retneC of Animal Models for Human Dis-
JINc;-TYAN MA (5), CLONTECH Labora-
,sesae ehT etutitsnI of lacideM ,ecneicS ehT
,seirot lnc, Palo Alto, California -30349
ytisrevinU of Tokyo, ,iadenakorihS Minato-
0324
kit, Tokyo ,9368-801 Japan
MARKUS KX~INAM (6). MRC Laboratory for
JEAN-CLAUDE NICOLAS (12). Montpellier
raluceloM Cell Biology, isrevinU ,O egelloC
School of ,enicideM INSERM ,934-U 09043
London, London WC1E 6BT, England
,reilleptnoM ecnarF
J. MARS~tALL (19), Brown ,ytisrevinU Provi- IKUYAS~H OHATA (29), Showa ,ytisrevinU
,ecned Rhode Island 21920 Tokyo ,241 Japan
JtsclE M. MATnESON (20), Department of -oM J. B. OLMSTED (10, Department of ,ygoloiB
ralucel ,ygoloiborueN ehT btstitute of -ideM ytisrevinU of Rochester, Rochester, New
lac ,ecneicS ehT ytisrevinU of Tokyo, -orihS York 72641
kanedaL Minato-ku, Tokyo ,9368-801 KEJTt~ R. OLSOY (11), ,scimolleC Inc., -sttiP
napaJ ,hgrub Pennsylvania 83251
ILYA A. MAZO (28, 30), CLONTECH Labo- Go'VrFR1ED J. PALM (32), Department of
,seirotar Inc., Palo Alto, California -30349 Strucnlral Biology dna ,yhpargollatsyrC
0324 e~mitsnl of Molecular Biotechnology,
TAKAYUKI MICHIKAWA (20), Department of 54770-D Jena, Germany
Molecular Neurobiology, ehT Institute of AMY PARKER (25), Kuzell etutitsnI for Arthri-
lacideM ,ecneicS ehT ytisrevinU of Tokyo, sit and Infectious ,sesaesiD California -aP
Shirokanedai, Minam-ku, Tokyo 108- cific lacideM ,retneC naS ,ocsicnarF -rofilaC
,9368 Japan ain 51149
OKIHUSTAK A3IIHSOKIM (20), teNlangisoicllk~ ,PEBOR T D. POSSEE (33), NERC Institute of
Project, Exploratory Research for Ad- ygoloriV dna Environmental ,ygoloiborciM
decnav ,ygolonhceT Tokyo ,4600-351 Japan Oxford 1XO 3SR, United Kingdom
TINO QIAN (29), Department of Cell Biology
ATSUSnl 1KAWAYIM (20), Department of Mo-
dna Anatomy, School of ,enicideM -revinU
ralucel ,ygoloiborueN ehT etutitsnI of -ideM
ytis of North Carolina--Chapel Hill,
Iac ,ecneicS ehT ytisrevinU of Tokyo, -orihS
Chapel Hill, North aniloraC 99572
kanedai, Minato-ku, Tokyo ,9368-801
napaJ P. PRABHAKARA REDDI (24), Department of
Cell Biology, ytisrevinU of ,ainigriV -rahC
SIMON G. .FELLOM (27), Centre for Plant -icS
,ellivsettol Virginia 80922
,secne ytisrevinU of ,sdeeL Leeds LS2 ,TJ9
ORNA RESNEKOV (13), Laboratory of -uceloM
detinU Kingdom
ral Genetics, National Institute of Child
REBECCA R. MULDOON (28, 30, 35), -uceloM htlaeH and Human Development, National
ral Oncology Laboratory, Human Gene setutitsnI of Health, Bethesda, Maryland
Therapy Research Instilute, Iowa Health 5872-29802
System, seD ,+enioM Iowa 2023-90305 FELIX J. SANGARI (25), Kuzell Institute for
AKIRA MUTO (20), langisoiclaC Net ,tcejorP Arthritis dna Infectious ,sesaesiD ainrofilaC
yrotarolpxE hcraeseR for Advanced -hceT cificaP Medical ,retneC naS ,ocsicnarF -ilaC
nology, Tokyo ,4600-351 Japan fornia 51149
xii CONTRIBUTORS TO VOLUME 302
JEAN M. SANGER (15), Department of Cell KENAN K. TURNACIOGLU (15), Department of
and Developmental Biology, University of Cell and Developmental Biology, -revinU
Pennsylvania School of ,enicideM -ledalihP sity of Pennsylvania School of ,enicideM
phia, Pennsylvania 8506-40191 ,aihpledalihP Pennsylvania 8506-40191
JOSEPH W. SANGER (15), Department of lleC KAZUHIKO UMESONO (37), Institute for Virus
dna Developmental Biology, University of Research, Kyoto University, Shogoin,
ainavlysnneP School of ,enicideM -ledalihP Sakyo-ku, Kyoto ,7058-606 Japan
phia, Pennsylvania 8506-40191 PETER E. URWIN (27), Centre for Plant Sci-
LEE G. SAYERS (20), Department of raluceloM ,secne ytisrevinU of Leeds, Leeds LS2 ,TJ9
Neurobiology, ehT Institute of Medical -icS detinU Kingdom
ence, ehT University of Tokyo, Shiro- ALAN S. VERKMAN (22), ralucsavoidraC Re-
kanedai, Minato-ku, Tokyo ,9368-801 search Institute, University of ,ainrofilaC
napaJ naS Francisco, naS Francisco, California
TATIANA SEREGINA (35), Molecular Oncol- 1250-34149
ogy Laboratory, Human Gene Therapy -eR SUMING WANG (35), Molecular Oncology
hcraes Institute, Iowa Health System, seD Laboratory, Human Gene Therapy -eR
Moines, Iowa 2023-90305 hcraes Institute, Iowa Health System, Des
Moines, Iowa 2023-90305
O~MUS SUGANO (21), Department of ,ygoloriV
ehT Institute of Medical ,ecneicS ehT -inU HIROYUKI WATABE (23), Department of Bio-
ytisrev of Tokyo, Shirokanedai, Minato-ku, ,yrtsimehc Faculty of Pharmaceutical Sci-
Tokyo ,9368-801 Japan ,secne Health Sciences University of Hok-
kaido, Ishikari-Tobetsu, Hokkaido 061-
CHARLES SULTAN (12), Montpellier School of
,3920 Japan
,enicideM 1NSERM ,934-U 34090 Montpel-
,reil ecnarF CHRIS D. WEBB (13), Information ygolonhceT
,eciffO Defense Advanced Research Proj-
TATSUYUKI TAKADA (21), Department of Ex-
stce Agency, Arlington, ainigriV 4171-30222
perimental Radiology, Shiga University of
Medical Science, Ohtsu, Shiga ,2912-025 NICOLA NOSN~KLTW (33), School of lacigoloiB
napaJ and Molecular Sciences, Oxford Brookes
,ytisrevinU Oxford OX3 OBP, United
B~ATR~CE TEROUANNE (12), Montpellier
Kingdom
School of ,enicideM INSERM ,934-U 09043
ALEXANDER WLODAWER (32), Macromo-
,reilleptnoM ecnarF
ralucel Structure Laboratory, ABL-Basic
CAROLE J. THOMAS (33), School of lacigoloiB Research Program, National Cancer In-
and Molecular Sciences, Oxford Brookes stitute-Frederick Cancer hcraeseR and -eD
,ytisrevinU Oxford OX3 OBP, United velopment Center, Frederick, Maryland
Kingdom 20712
DONNA R. TROLLINOER (29), Department of KENICHI YOStlIDA (21), Department of -loriV
lleC Biology and Anatomy, School of -ideM ogy, The Institute of Medical Science, ehT
,enic ytisrevinU of North Carolina--Chapel ytisrevinU of Tokyo, ,iadenakorihS Minato-
Hill, Chapel Hill, North Carolina 99572 ku, Tokyo ,9368-801 Japan
KCIREDERF I. TSUJ~ (37), Scripps Institution JIE ZHANO (14), Howard Hughes Medical In-
of ,yhpargonaecO ytisrevinU of ,ainrofilaC stitute and Department of Cell Biology,
naS Diego, La Jolla, ainrofilaC 2020-39029 Duke University, Durham, North Caro-
anil 01772
GozoH TSUJIMOTO (211, Department of Mo-
,ralucel Cell Pharmacology, National -lihC XIAONING ZHAO (4, 36), CLONTECH Labo-
dren's Medical Research ,retneC ,odihsiaT ,seirotar Inc., Palo Alto, California -30349
,uk-ayagateS Tokyo ,9058-451 Japan 0324
Preface
This Methods ni Enzymology volume deals with the utility of green
fluorescent protein (GFP). The OVID database (including MEDLINE,
Current Contents, and other sources) lists nine references to GFP for the
ten-year period 1985-1994. In contrast, in less than four years thereafter,
more than 500 are listed, a testament to the rapid growth of interest in
this probe.
This volume documents many diverse uses for this interesting molecule
in disciplines that broadly span biology. The methods presented include
shortcuts and conveniences not included in the sources from which they
were taken. The techniques are described in a context that allows compari-
sons to other related methodologies. The authors were encouraged to do
this in the belief that such comparisons are valuable to readers who must
adapt extant procedures to new systems. Also, so far as possible, methodolo-
gies are presented in a manner that stresses their general applicability and
potential limitations. Although for various reasons some topics are not
covered, the volume provides a substantial and current overview of the
extant methodology in the field and a view of its rapid development.
Particular thanks go to the authors for their attention to meeting dead-
lines and for maintaining high standards of quality, to the series editors
for their encouragement, and to the staff of Academic Press for their help
and timely publication of the volume.
P. MICHAEL CONN
iiix
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited yb SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
Edited yb SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV III. Preparation and Assay of Substrates
Edited yb SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. COLOWICK AND NATHAN O, KAPLAN
EMULOV ,V Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited yb SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited yb ELIZABETH F. NEUFELD DNA VICTOR GINSBURG
VOLUME IX. Carbohydrate Metabolism
Edited yb SILLIW A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited yb RONALD W. ESTABROOK DNA MAYNARD E. PULLMAN
VOLUME XI. Enzyme Structure
Edited by .C H. W. SRIH
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE GROSSMAN DNA KIVIE MOLDAVE
EMULOV XIII. Citric Acid Cycle
Edited by J. ,M N1ETSNEWOL
VOLUME XIV. Lipids
Edited yb J. .M N1ETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
VX
xvi
METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by HERBERT TABOR DNA CELIA WHITE TABOR
EMULOV XVII1. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
EMULOV XIX. Proteolytic Enzymes
Edited yb GERTRUDE E. NNAMLREP DNA LORAND LASZLO
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by LAWRENCE NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by SAN PmTRO ANTHONY
EMULOV XXiV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by SAN PIETRO ANTHONY
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. Hms DNA SERGE N. EFEHSAM1T
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H, W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA SERGE N. TIMASHEFF
EMULOV XXVIll. Complex Carbohydrates (Part B)
Edited by VICTOR GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by LAWRENCE NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVtE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by FLEISCHER SIDNEY DNA PACKER LESTER
EMULOV XXXII. Biomembranes (Part B)
Edited yb FLEISCHER SIDNEY DNA LESTER PACKER
EMULOV XXXIII. Cumulative Subject Index Volumes 1-XXX
Edited yb MARTHA G. DENNIS DNA EDWARD A. DENNIS
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. JAKOBY DNA MEIR WILCHEK
EMULOV XXXV. Lipids (Part B)
Edited yb JOHN M. NIETSNEWOL
METHODS NI ENZYMOLOOY xvii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL O. HARDMAN
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by TREB W. O'MALLEY DNA LEOJ G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL O. HARDMAN DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XL, Hormone Action (Part E: Nuclear Structure and Function)
Edited by TREB W, O'MALLEY DNA JOEL G. HARDMAN
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited yb W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W, A. DOOW
EMULOV XLIII. Antibiotics
Edited by JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. JAKOBY DNA MEIR KEHCL1W
EMULOV XLVII, Enzyme Structure (Part E)
Edited by ,C H. W. HIRS DNA EGRES N, FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C, H. W. HIRS DNA EGRES N. FFEHSAM~T
EMULOV XLIX. Enzyme Structure (Part G)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAM1T
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by PATRICIA A. HOFFEE DNA MARY ELLEN JONES
EMULOV LII, Biomembranes (Part C: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIII, Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by YENDIS REHCS1ELF DNA RETSEL REKCAP
xviii METHODSIN ENZYMOLOGY
EMULOV LV, Biomembranes (Part F: Bioenergetics)
Edited yb SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited yb ENELRAM A. DELucA
EMULOV LVIII. Cell Culture
Edited by WILLIAM B. JAKOBY DNA IRA PASTAN
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVlE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV .16 Enzyme Structure (Part H)
Edited by .C H. W. HIRS DNA EGRES .N FFEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited yb DONALD B. McCORMICK DNA LEMUEL D. WRIGHT
EMULOV .36 Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
Edited by DANIEL L. PURICH
EMULOV .46 Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and Com-
plex Enzyme Systems)
Edited by LEINAD L. HC1RUP
EMULOV .56 Nucleic Acids (Part I)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited by DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited by DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
VOLUME 68. Recombinant DNA
Edited by YAR Wu
EMULOV .96 Photosynthesis and Nitrogen Fixation (Part C)
Edited by ANTHONY SAN PIETRO
EMULOV .07 Immunochemical Techniques (Part A)
Edited by HELEN VAN VUNAKIS DNA JOHN J. LANGONE
EMULOV .17 Lipids (Part C)
Edited by NHOJ .M NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited by NHOJ .M NIETSNEWOL
METHODS NI ENZYMOLOGY xix
EMULOV .37 hnmunochemical Techniques (Part B)
Edited by NHOJ J. ENOGNAL DNA NELEH NAV SIKANUV
EMULOV .47 Immunochemieal Techniques (Part C)
Edited by JOHN J. LANGONE DNA HELEN VAN VUNAKIS
EMULOV .57 Cumulative Subject Index Volumes XXXI, XXXII, XXXIV-LX
Edited by EDWARD A. DENNIS DNA MARTHA G. DENNIS
VOLUME 76. Hemoglobins
Edited by ERALDO ANTONINI, LUIGI ROSSI-BERNARDI, DNA EMIL1A CHIANCONE
EMULOV .77 Detoxication and Drug Metabolism
Edited by WILLIAM B. JAKOBY
EMULOV .87 lnterferons (Part A)
Edited by YENDIS AKTSEP
EMULOV .97 Interferons (Part B)
Edited by YENDIS AKTSEP
EMULOV .08 Proteolytic Enzymes (Part C)
Edited by OLZSAL DNAROL
EMULOV .18 Biomembranes (Part H: Visual Pigments and Purple Mem-
branes, l)
Edited yb RETSEL REKCAP
EMULOV .28 Structural and Contractile Proteins (Part A: Extracellular Matrix)
Edited by NOEL W. MAHONINNUC DNA DIXIE W. NESKIREDERF
EMULOV .38 Complex Carbohydrates (Part D)
Edited by ROTCIV GRUBSNIG
EMULOV .48 Immunochemical Techniques (Part D: Selected Immunoassays)
Edited by JOHN J. LANOONE DNA HELEN VAN VUNAKIS
EMULOV .58 Structural and Contractile Proteins (Part B: The Contractile Appara-
tus and the Cytoskeleton)
Edited by DIXIE W. FREDERIKSEN DNA LEON W. MAHGN1NNUC
EMULOV .68 Prostaglandins and Arachidonate Metabolites
Edited by WILLIAM E. S. LANDS DNA WILLIAM L. SMITH
EMULOV .78 Enzyme Kinetics and Mechanism (Part C: Intermediates, Stereo-
chemistry, and Rate Studies)
Edited by DANIEL L. PURICH
EMULOV .88 Biomembranes (Part I: Visual Pigments and Purple Mem-
branes, lI)
Edited by RETSEL REKCAP
EMULOV .98 Carbohydrate Metabolism (Part D)
Edited by WILLIS A. WOOD
EMULOV .09 Carbohydrate Metabolism (Part E)
Edited by SILLIW A. DOOW