Global Strategies for Disease Detection and Treatment (Disease Markers) PDF
115 Pages·2002·15.137 MB·English
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The field of proteomics holds great promise for identifying non-targeted, global molecular profiles "signatures" of normal and diseased cells. While genomics offers an array of tools for identifying mutated or dysregulated genes, proteonomics offers the ability to measure post-translational modifications, protein stability, phosphorylation state, protein-protein interactions, and protein DNA-binding affinities. This work shows that strategies based on the separate analysis of subcellular compartments provide the means to determine protein location in a cell. Selective enrichment for a subset of proteins of interest can be achieved using a variety of techniques from centrifugation procedures to affinity capture.
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