Germ-Free Biology Experimental and Clinical Aspects: Proceedings of an International Symposium on Gnotobiology held in Buffalo, New York, June 9–11, 1968 PDF
Preview Germ-Free Biology Experimental and Clinical Aspects: Proceedings of an International Symposium on Gnotobiology held in Buffalo, New York, June 9–11, 1968
GERM-FREE BIOLOGY Experimental and Clinical Aspects ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY Editorial Board: Nathan Back Chairman, Department 01 Biochemical Pharmacology, School 01 Pharmacy, State University 0/ New York, BuDalo, New York N. R. Di Luzio Chairman, Department 0/ Physiology, Tulane Un iversity School 0/ M edicine, N ew Orleans, Louisiana Alfred GeIlhorn University 0/ Pennsylvania Medical School, Philadelphia, Pennsylvania Bernard Halpern Director 0/ the Institute 0/ Immuno-Biology, Paris, France Ephraim Katchalski Department 01 Biophysics, The Weizmann Institute 0/ Science, Rehovoth, Israel David Kritchevsky Wistar Institute, Philadelphia, Pennsylvania Abel Lajtha N ew York State Research Institute lor N eurochemistry and Drug Addiction, Ward's Island, New York Rodolfo Paoletti Institute 0/ Pharmacology, University 0/ Milan, Milan, [taly, and Institute 0/ Pharmacology, University 01 Cagliari, Cagliari, Italy Volume 1 l'HE RETICULOENDOTHELIAL SYSTEM AND ATHEROSCLEROSIS Edited by N_ R. Di Luzio and R. Paoletti • 1967 Volume2 PHARMACOLOGY OF HORMONAL POLYPEPTIDES AND PROTEINS Edited by N. Back, L. Martini, und R. Paoletti • 1968 Volume3 GERM-FREE BIOLOGY - EXPERIMENTAL AND CLINICAL ASPECTS Edited by E. A. Mirand and N. Back· 1969 Volume 4 DRUGS AFFECTING LIPID METABOLISM Edited by W. 1. Holmes, 1. A. Carlson, und R. Paoletti • 1969 GERM-FREE BIOLOGY Experimental and Clinical Aspects Proceedings of an International Symposium on Gnotobiology held in Buffalo, New York, June 9-11, 1968 Edited by Edwin A. Mirand Roswell Park Memorial Institute New York State Departmentol Health State University 01 New York, BufJalo, New Yorle N iagara U niversity, N iagara U niversity, N ew Y orle Canisius College, BufJalo, N ew Y orle and Nathan Back Department 0/ Biochemical Pharmacology School 0/ Pharmacy State University 01 New Y ork BufJalo, New York <:±? Springer Science+Business Media, LLC 1969 Library of Congress Catalog Card Number 69-16518 © 1969 Springer Science+Business Media New York Origina11y published by Plenum Press, New York in 1969. All rights reserved No part of this publication may be reproduced in any form without written permission from the publisher ISBN 978-1-4899-6234-8 ISBN 978-1-4899-6495-3 (eBook) DOI 10.1007/978-1-4899-6495-3 Softcover reprint of the hardcover 1s t edition 1969 PREFACE The purpose of this publication is to provide a cross-sectional version of the present state of gnotobiology and its clinical ap- plications by biomedical scientists and physicianso With contri- butions from abroad as well as from the United States, the current status of gnotobiology is amply exploredo We wish to express our sincere appreciation to the participants for their contributions to this Symposium held in Buffalo, New York on June 9 - 11, 1968, sponsored by Roswell Park Memorial Institute, State University of New York at Buffalo School of Pharmacy, and the Association for Gnotobiotics, Inc o We owe a great debt to those pioneers who labored under great hardships to develop this area of science o Such people at the Lobund Institute at the University of Notre Dame, notably the late Professor J. Ao Reyniers and Professor Po Co Trexler and their colleagues as well as others in Sweden, Japan and elsewhere, are to be recognized for their enormous effort to keep this field aliveo Who can determine the limits of the usefulness of gnotobiotic tech- niques in solving problems in a number of the scienceso Its useful- ness in immunological research is becoming more clearly understoodo The techniques in gnotobiotic research may be useful too in aero- space scienceo To those working in gnotobiology, Dro Nathan Back and I would like to commend all of you who have contributed to so many i.mportant developments in understanding the biological rela- tionships between animals including man and their microflorao Edwin Ao Mirand December, 1968 v CONTENTS PATIENT CARE IN STERILE ENVIRONMENT The App1ication of Laminar F10w Rooms to Patient Isolation • • • • 0 • • • 0 • 0 1 Dona1d Ves1ey, George S. Michaelsen, and A1exander A. Levitan Technica1 Advances in Patient Isolation ••••• o • " 0 • 11 R. P. Hummel, M. Ma1ey, and B. G. MacMi11an Laminar Air F10w Unit for Patients Undergoing Cancer Chemotherapy • • • • • • • • • • • • • • • 19 Gera1d P. Bodey Psychologie Adaptation of Patients During Treatment of Acute Leukemia in Life Is1and Isolator 27 Les1ie Fine, Morton Wachspress, David N. Graubert, John Edmonson, and Howard Eisman Fever and Infection in Patients Treated with Intensive Antineop1astic Chemotherapy in an Isolator 35 John H. Edmonson, Wi1frido M. Sy, Stephen J. Millian, and Stan1ey L. Lee Gnotobiotic Man • • • 45 Rona1d D. Barnes EXPERIMENTAL BIOLOGY Penicillin Prophy1axis of Post-Operative C1ostridia1 Gangrene in Monocontaminated Guinea Pigs 63 Bruce P. Phi11ips Intestinal Monocontamination in the Neonata1 Pig: Microbio1ogica1 and Microscopic Studies 65 T. E. Sta1ey, E. W. Jones, and L. D. Cor1ey ~i viii CONTENTS The Peribronchial Lymphocytic Tissue in Germfree, Defined-Flora, Conventional and Chronic Murine Pneumonia-Affected Rats • • 75 W. E. Giddens, Jr. and C. K. Whitehair Bacterial Burden and Wound Repair in Conventional and Germfree Rats •••••••••• 85 David T. Rovee, Carole A. Kurowsky, and Patricia A. Flanagan Effect of Type of Diet on Cholesterol Absorption and Bile Salt Excretion in Germfree and Conventional Chicks • • • • • • • • • 97 H. Eyssen, G. Van Messom, and J. Van den Bosch Infectivity of Avian Reoviruses for Day-Old Chicks Under Germ-free and Isolation Conditions 107 D. R. Deshmukh and B. S. Pomeroy Erythropoietic Response of Germfree Mice to Erythropoietin • • • • • • • • • • 111 Edwin A. Mirand, Albert S. Gordon, and Gerald P. Murphy LEUKEMIA IN GERMFREE RESEARCH Viral Status of Germfree Rodents; Present and Future 117 Masahiro Kajima Leukemia in Germfree AKR Mice • • • • • 125 H. Ira Pilgrim, Richard C. Parks, and Lloyd W. Law Reticular Neoplasms in Irradiated and Unirradiated Germfree Mice • • • • • • • • • • 135 H. E. Walburg, Jr. and G. E. Cosgrove CARCINOGENESIS Comparison Between the Effects of a Single Dose Of_8 Chemical Carcinogen on Gnotobiotic and Conventional Mice • • • • • • • • • • o • • 0 143 G. A. Grant and F. J. C. Roe Rous Sarcoma Virus in Germfree Rats • • • • • • 0 • • • • • • 149 Morris Pollard, Masahiro Kajima, and Theodore P. Zacharia CONTENTS ix 7,12-Dimethy1benz(~) Anthracene-Induced Ma1ignant Lymphoma in Germfree Mice • • • • • • • 153 Masahiro Kajima Gnotobiotes as Usefu1 Too1s in Studies in Carcino- genesis with Cycasin, A Natural G1ucoside 163 Gert L. Laqueur PHYSIOLOGY OF CECUM Bidirectiona1 Permeation of Water and Amino Acids in the Cecum of Germfree Rats • • 169 Etiennette Combe and Helmut A. Gordon Recent Progress 'in Studies on a Bioactive Pigment ("Alpha Pigment") Iso1ated from Ceca1 Contents of Germfree Rodents ••••• 171 Helmut A. Gordon, Eszter Kokas, and Ju1ius Magyary-Kossa Kinin-Forming Activity in Ceca1 Contents of Germ- Free and Conventiona1 Mice • • • • 173 N. Back, R. Steger, and E. A. Mirand Effects of Clostridium Diffici1e, Lactobaci11us ~, Bacillus Subtilis and Lactobacillus sp. as Mono- and Dicontaminants on the Cecum of Germfree Mice •••• • • • • • • 179 Lasz16 Pesti, Eszter Kokas, and Helmut A. Gordon Simi1arities Between Germfree Mice and Mice with an Antibiotic Decontaminated Digestive Tract 181 D. van der Waaij The Effect of Intestinal Flora on Toxicity of Nitrogen Mustard •••••• 191 Judith A. O'Ma11ey, Joyce Jividen, Edwin A. Mirand, and James F. Holland DISEASE RESISTANCE OF GERMFREE ANIMALS Effects of the Normal Flora on Host Defenses Against Microbia1 Invasion 197 Gera1d D. Abrams Ageing and Resistance to Infection in Germfree C3Hf Mice • • • • • • • • • • • • • 207 Henry C. Outzen x CONTENTS Studies of the Effects of Immune Serum on the Course of ~ Coli Infections in Gnotobiotic Pigs 219 E. M. Koh1er Differentiation of Potentia11y Immunocompetent Ce11s in the Mouse • • • • • • • • • 227 G. Cudkowicz and G. M. Shearer A Comparative Study of the Immune Reaction in Germfree and Conventiona1 Mice • • • • • • • • • 237 M. G. Hanna, Jr., P. Nettesheim, and H. E. Walburg, Jr. Deve10pment of Immunologie Competence in Germfree and Conventiona1 Mice • • • • • • • • • • • • • 249 M. J. Bosma IMMUNOLOGY The True PrimaryImmune Response in Germfree Co1ostrum-Deprived Pig1ets 259 Yoon Berm Kim and Dennis W. Watson Hemo1ytic P1aque-Forming Ce11s and Antigen-Sensitive Units in Spleens of Germfree and Conventiona1 Mice • • • 269 G. M. Shearer, G. Cudkowicz, and H. E. Walburg, Jr. Severity of the Parent to F1 Graft-Versus-Host Reaction in Irradiated Germfree Mice 277 H. E. Walburg, Jr. and G. E. Cosgrove Diet and Immune Mechanisms • o 0 0 • • 0 • 0 • 287 B. S. Wostmann, J. R. P1easants, and Patricia Bealmear NUTRITION The Response of Germfree Rats to Dietary Cho1estero1 293 Themas Ke110gg and Bernard Wostmann The Absorption of Fat by Germfree and Conventionalized Ra ts 0 0 0 • • • • 0 0 0 0 0 0 • 0 • 0 297 Mario Reina-Guerra, B. Tennant, D. Harro1d, and M. Goldman
The list of books you might like
