Geographical Distribution of Sexual and Apogamous Types of Dryopteris chinensis (Dryopteridaceae) in Japan

2015·5.2 MB·English

by  Hori, Kiyotaka

The Japanese Society for Plant Systematics
ISSN 1346-7565 Acta Phytotax. Geobot. 66 (1): 35–45 (2015)
Geographical Distribution of Sexual and Apogamous Types of
Dryopteris chinensis (Dryopteridaceae) in Japan
KiyotaKa Hori1, MeguMi MatsuMoto1, atsusHi ebiHara2, NaruMi NaKato3
aNd NoriaKi MuraKaMi1
1Makino Herbarium, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji,
Tokyo 192-0397, Japan. *[email protected] (author for correspondence);
2Department of Botany, National Museum of Nature and Science, 4-1-1 Amakubo,
Tsukuba, Ibaraki 305-0005, Japan; 3Narahashi 1–363, Higashiyamato-shi, Tokyo,
207–0031 Japan
Only triploid and tetraploid apogamous cytotypes have previously been reported for the fern Dryopteris
chinensis (Dryopteridaceae). In this paper we report the discovery of a diploid sexual cytotype of D. chi-
nensis. The geographical distribution of the sexual and apogamous types of the species in Japan and ad-
jacent region was determined by counting the number of spores per sporangium in a large number of
herbarium specimens from MAK and TNS. Apogamous plants were found to be the predominant type in
Japan; the sexual type was restricted to Miyazaki, Oita, and Kumamoto prefectures in Kyushu, and Ko-
chi Prefecture in Shikoku, Japan.
Keywords: apogamy, chromosome number, Dryopteridaceae, Dryopteris chinensis, reproductive mode,
sexual cytotype
The fern Dryopteris chinensis (Baker) Koidz. Zhejiang provinces (Zhang 2012). Weng (1989)
(Dryopteridaceae), considered to be apogamous, reported triploid apogamous plants of D. chinensis
is widely distributed in China and Japan (Zhang from Lushan in Jiangxi Province, and Nakato et
2012, Iwatsuki et al. 1995). Only triploid apoga- al. (1995) reported triploid and tetraploid apoga-
mous types of D. chinensis have been reported mous D. chinensis from Jiangsu Province. In Ja-
from Japan, whereas tetraploid apogamous plants pan, triploid apogamous plants were reported
have been reported from China (Kurita 1961, Hi- from Saitama and Shizuoka prefectures, Honshu
rabayashi 1966, 1974, Mitui 1968, Kurata & Na- (Kurita 1961, Hirabayashi 1966, Mitui 1968, Hi-
kaike 1985, Weng 1989, Nakato et al. 1995). Lee rabayashi 1974), but D. chinensis is also widely
& Park (2013) recently reported apogamous D. distributed in Honshu, Shikoku, and Kyushu. We
chinensis from Korea, although its ploidy level therefore suspected that sexual cytotypes might
was unknown. To the best of our knowledge, sex- occur.
ual plants of D. chinensis have never been report- During this study we discovered diploid sexu-
ed. Previous cytological studies were based on al plants of Dryopteris chinensis in Miyazaki
plants collected from only a few localities or ar- Prefecture, Kyushu, Japan, and also updated the
eas throughout its range. distribution of sexual and apogamous types of D.
In China, Dryopteris chinensis occurs in An- chinensis in Japan, mainly by counting the num-
hui, Guangxi, Hebei, Henan, Hubei, Hunan, Ji- ber of spores per sporangium in herbarium speci-
angsu, Jiangxi, Jilin, Liaoning, Shandong, and mens.
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Methods of ploidy analyses using flow cytom-
Materials and Methods etry followed Hori et al. (2014). To determine the
ploidy level, the DNA content (2C value) of each
Plant Materials nucleus extracted from fresh pinnae was mea-
One living stock and 19 leaf samples of sured by flow cytometry using a Cyflow Ploidy
Dryopteris chinensis were collected in eight lo- Analyzer PA-II (Partec, Munster, Germany) and
calities in Miyazaki Prefecture, Japan, on June a Cystain UV Precise P kit (Partec). Approxi-
18–20, 2013, and in one locality in Kumamoto mately 100 mm2 of each pinna was torn into sev-
Prefecture, Japan, on March 26, 2013. eral pieces and finely chopped with a razor blade
in 0.25 mL of nucleus extraction buffer from the
Cytological observation and determination of kit. Then, 0.8 mL of staining solution from the kit
reproductive mode was added to the chopped tissues. The crushed
Root tips were pretreated with 0.004 M 8-hy- tissue and buffers were filtered through a 30-μm
droxyquinoline for 6 h at approximately 17–20°C nylon mesh (Partec). Fresh leaf tissues of Nicoti-
to observe mitotic chromosomes. After fixation ana tabacum L. (approximately 25 mm2) and
in ethanol and acetic acid (3:1) for 15–30 min, the Dryopteris erythrosora (D. C. Eaton) Kuntze (ap-
root tips were hydrolyzed in 1 N HCl at 60°C for proximately 100 mm2) were used as internal stan-
1–3 min and squashed in 2% aceto-orcein solu- dards. The preliminary analysis revealed that the
tion. The chromosomes were then observed un- nuclear DNA content peaks of D. chinensis and
der a Nikon OPTIPHOT microscope and photo- N. tabacum overlapped on the ploidy analyzer.
graphed with a Panasonic LUMIX DMC-TZ5 We therefore determined the genome size of D.
digital camera. erythrosora by using N. tabacum as an internal
To determine the reproductive mode of each standard, then used D. erythrosora as the refer-
sample, the spores in one sporangium were count- ence to estimate the genome size of D. chinensis.
ed. If the number was 64, we determined the sam-
ple to be sexually reproducing; if 32, then we de- Identification of reproductive mode of herbarium
termined the sample to be apogamous (Manton specimens
1950). Four hundred and seventy six specimens re-
taining unburst sporangia from approximately
Ploidy analyses 2,000 sheets of Dryopteris chinensis in the her-
Fig. 1. Photograph (left) and schematic drawing (right) of mitotic metaphase chromosomes of a diploid sexual individual of
Dryopteris chinensis (K. Hori Dpaci 856-2, MAK 412500). Scale bar, 5 μm.
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February 2015 Hori & al.—Sexual type of Dryopteris chinensis 37
same way as for the living samples.
Results
Ploidy and reproductive mode
Figure 1 shows a photograph and a diagram-
matic drawing of mitotic metaphase chromo-
somes in one individual of Dryopteris chinensis
(K. Hori Dpaci 856-2, MAK 412500). Based on
the chromosome number of 2n = 82 and 64 spores
per sporangium (Fig. 2), we determined this sam-
ple to be diploid and sexual.
The genome the diploid sexual individual of
Fig. 2. Spores in a sporangium of a diploid sexual individual
of Dryopteris chinensis (K. Hori Dpaci 856-2, MAK Dryopteris chinensis was 0.50 times the internal
412500). Scale bar, 1 mm. standard of D. erythrosora, while D. erythrosora
was 2.26 times that of Nicotiana tabacum in our
ploidy analyses. Considering that the genome
size (2C value) of N. tabacum is approximately
barium of the National Museum of Nature and 11.7 pg (Narayan 1987), the genome size of D.
Science (TNS) and the Makino Herbarium of To- chinensis (K. Hori Dpaci 856-2, MAK 412500) is
kyo Metropolitan University (MAK) were select- estimated to be approximately 13.2 pg. Among 19
ed to determine the reproductive mode. The num- leaf samples collected in 2013, all except one had
ber of spores per sporangium was counted under 64 spores per sporangium, and were thus estimat-
a stereomicroscope. The type of reproduction, ed to be sexual reproducing. The genome size of
apogamous or sexual, was determined in the 15 sexual plants were determined to be 12.5–14.5
Fig. 3. Distribution of apogamous and sexual types of Dryopteris chinensis based on spore counts of herbarium specimens.
Black circle, sexual type; gray circle, apogamous type.
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February 2015 Hori & al.—Sexual type of Dryopteris chinensis 39
Fig. 5. Habitat of diploid sexual Dryopteris chinensis in Kitagawa-cho, Nobeoka-shi, Miyazaki Prefecture (K.
Hori Dpaci 856-2, MAK 412500). A, Semi shade under trees beside a highway; B, Sexual D. chinensis on
cracked rock.
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pg (Appendix 1), which is exactly the same in the and Deparia minamitanii Serizawa (Iwatsuki et
diploid Dpaci 856-2 (13.2 pg) [K. Hori Dpaci 816 al. 1995).
(MAK 410320) ~ Dpaci 860 (MAK 410359)]. We In angiosperms, asexually reproducing cyto-
estimated one individual of Dryopteris chinensis types usually have a wider and more northern
[K. Hori Dpaci 813 (MAK 410317)] with 32 distribution than their sexual relatives and has
spores per sporangium and a genome size of 19.5 been referred to as “geographical parthenogene-
pg to be triploid and apogamous. sis” (Hörandl 2006). This phenomenon has not
been well documented in ferns, but the apoga-
Reproductive mode of herbarium specimens mous Asplenium unilaterale has been reported to
Spore number per sporangium was deter- occur in more northern areas (Murakami & Iwat-
mined for 476 sheets of selected specimens. suki 1983). Dryopteris caudipinna (a diploid sex-
Among them, 39 specimens had 64 spores per ual species) occurs in more southern areas than
sporangium and 437 specimens had 32 spores per D. erythrosora, a triploid apogamous species
sporangium (Appendix 1). We were unable to closely related to D. caudipinna (Yamamoto et al.
count the spores of the remaining specimens. 2010). The results of our study suggest that geo-
Based on the source of the specimens, the dis- graphical parthenogenesis is common also in
tribution of the sexual and apogamous types of ferns.
Dryopteris chinensis in Japan are shown in Fig. 3. In the present study, diploid sexual Dryopter-
We noticed no significant morphological differ- is chinensis was observed to occur only near the
ences between the apogamous and sexual cyto- southern limit of distribution of the apogamous
types (Fig. 4). type in Japan. D. chinensis is also in China,
where the sexual type may be more widely dis-
Habitats of the sexual diploid Dryopteris tributed. A wider survey, including plants from
chinensis China, is recommended.
The diploid sexual individuals of Dryopteris
chinensis were from cracked rocks in semi shade We are grateful to the following persons for their assis-
tance in collecting plant materials: Mr. Y. Inoue, Mr. T.
under trees beside highways (Fig.5). The apoga-
Minamitani, Mr. K. Akagi and Mr. T. Oka of the Nippon
mous individuals were often in similar habitats,
Fernist Club. This study was partly supported by a Grant-
but mixed sexual and apogamous populations
in-Aid for JSPS Fellows No. 26-1720 to K. Hori and by a
were not found. Grant-in-Aid for Scientific Research No. 25291089 to N.
Murakami.
Discussion
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e modes, ploidies and voucher inf LocalityDateYearTowns, villages, mountains Hata-gunn, Nishitosa-mura, Kurosonn riverJul. 291980Aug. 121984Kamimashiki-gunn, Seiwa-mura, DaikannyamaYatsushiro-shi, Izumi-machi, ShiibaruMar. 262013Nobeokashi, HasekawauchiSep. 121982Nishiusuki-gunn,Hinokage-cho, MitateNov. 231982Nishiusuki-gunn,Hinokage-cho, KamikageOct. 151982Nobeoka-shi, TaeSep. 121982Koyu-gunn, Kijo-cho, KobudokoroSep. 51982Koyu-gunn, Kijo-cho, IshikawachiFeb. 141964Nobeoka-shi, Houri river- Mt. OkueAug. 161960Nobeoka-shi, Houri river- Mt. OkueAug. 161960Jun. 192013Nobeoka-shi, Kitakata-cho, ShimoshishigawaNishiusuki-gunn,Hinokage-machi, MitateJun. 192013Nobeoka-shi, Hori-riverJun. 182013Nobeoka-shi, Hori-riverJun. 182013Nobeoka-shi, Kitakata-cho, SugebaruJun. 192013Nobeoka-shi, Kitakata-cho, SugebaruJun. 192013Nobeoka-shi, Kitakata-cho, SugebaruJun. 192013Nobeoka-shi, Kitakata-cho, SugebaruJun. 192013Nobeoka-shi, Kitakata-cho, SugebaruJun. 192013Nobeoka-shi, Kitagawa-cho, KawachimyoJun. 182013Nobeoka-shi, Kitagawa-cho, KawachimyoJun. 182013Nobeoka-shi, Kitagawa-cho, KawachimyoJun. 182013Nobeoka-shi, Kitagawa-cho, KawachimyoJun. 182013Hyuuga-shi, Togo-cho, ShimosanngeJun. 202013Hyuuga-shi, Togo-cho, ShimosanngeJun. 202013Hyuuga-shi, Togo-cho, ShimosanngeJun. 202013Hyuuga-shi, Togo-cho, ShimosanngeJun. 202013Nobeoka-shi, Kitagawa-cho, KawachimyoJun. 182013Nobeoka-shi, Kitakata-cho, SugebaruJun. 192013Nov. 101996Higashiusuki-gunn, Morotsuka-mura, Iiboshi passNov. 101996Higashiusuki-gunn, Morotsuka-mura, Iiboshi passNov. 101996Higashiusuki-gunn, Morotsuka-mura, Iiboshi passNov. 101996Higashiusuki-gunn, Morotsuka-mura, Iiboshi passHyuuga-shi, Togo-cho, Ishinami riverNov. 52005Nobeoka-shi, Mt. OkueJul. 292006Jul. 311962Ono-gunn, Ogata-machi, Mt. KatabukiOno-gunn, Ogata-machi, Mt. SoboAug. 1955Ono-gunn, Mie-machi, Hakusanumezu passJul. 301977Seto-shi, KamihadagawaAug. 281980Inuyama-shi, TsugaoJul. 131980Nishio-shi, Hirahara-choOct. 171982Higashikamo-gunn, Asuke-cho, OtagaAug. 121978Minamishitara-gunn, Horai-choJul. 241983Toyota-shi, Obata-choAug. 151978Minamishitara-gunn, Tsukude-muraAug. 31983Seto-shi, Joko-jiDec. 11974Minamishitara-gunn, Horai-choJul. 221975Kitaakita-gunn, Ani-machiOct. 71981Hachinohe-shi, Nanngo-ku, AotsukuriAug. 242006Ichihara-shi, KayahashiOct. 231983Katori-gunn, Shimousa-machi, NagiAug. 161981
productiv County, PrefectureKochi Pref.Kumamoto Pref.Kumamoto Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Oita Pref.Oita Pref.Oita Pref.Aichi Pref.Aichi Pref.Aichi Pref.Aichi Pref.Aichi Pref.Aichi Pref.Aichi Pref.Aichi Pref.Aichi Pref.Akita Pref.Aomori Pref.Chiba Pref.Chiba Pref.
1. RepeNdixspecimens. DNA Ploidy amounts levelCountry(pg.) JapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapan13.0 2xJapan13.92xJapan14.52xJapan13.72xJapan12.52xJapan13.52xJapan12.72xJapan13.62xJapan12.92xJapan14.52xJapan13.22xJapanJapan13.2Japan2x(2n=82)13.62xJapan13.52xJapan14.02xJapan13.62xJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapan
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Specimen no.Personal TNSMAKno.12049461204943448209 448206 448199 446716 250384 172881 146733 128406 01026250446914 446913 446912 446911 815296 815297 815304 1144092845742388331202352123557694 557709 1207591444499 1207589519365 519364 519376 519374 519373 519379 521115 519100 519355 519360 519366 521145 521151 521137 521135 521129 521126 521131 521132 521133 521122 521121 521119 521150 430815 430817
Coll. S. WakuS. WakuT. WakuT. NakaikeY. KobayashiS. WakuK. MasudaM. KuriharaT. TanakaT. WakanaT. IwataS. WakuS. WakuS. WakuS. WakuYukio JotauiYukio JotauiYukio JotauiT. MakinoN. KatoM. MizushimaK. MasudaM. TobeA. MinamiA. MinamiT. TakedaA. MinamiA. MinamiT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaM. MatsumuraT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaT. TakedaM. MatsumuraM. Matsumura
Year 198419851980198219801983196919661961195519851983198319831983196819681962190219841957196919571981198119751981198219771977197619741975197619761981197919771976197819751975197719751975197519761976197519741975197719811979
Date Sep. 25Oct. 22Nov. 4Jul. 4Jul. 20Jul. 12Aug. 20Nov. 7Sep. 14Jul. 13Oct. 20Oct. 15Oct. 15Sep. 16Jul. 19Jul. 14Jul. 14Oct. 9Sep. 7Aug. 6Oct. 11Oct. 27Sep. 28Oct. 17Oct. 17Jul. 20Oct. 17Oct. 10Jul. 26Oct. 22Jul. 27Sep. 10Sep. 6Dec. 4May. 1Sep. 15Nov. 15Oct. 31Nov. 9Sep. 9Oct. 14Jul. 15Oct. 24Sep. 10Sep. 5Sep. 20Aug. 3Aug. 8Aug. 29Aug. 9Jul. 28Oct. 16Aug. 10Sep. 23
Locality Towns, villages, mountains Seta-gunn, Kurohone-mura, KamikayamaAzuma-gunn, Nakanojo-machi, KamisawatariOgo-machi, NegoyaKiryuu-shi, TenjinKannra-gunn, shimonita-machi, TsuchiyazawaOra-gunn, Itakura-machi, EbizemineAgatsuma-gunn, Agatsuma-machiTakasaki-shi, Mt. KannonnMaebashi-shiTone-gunn, Azuma-muraKozuke-mura, Mt. SuwaSeta-gunn, Kurohone-cho, SyukumeguriSeta-gunn, Azuma-muraSeta-gunn, Azuma-muraKiryuu-shi, Hirosawa-choKoudukeichinomiyaKoudukeichinomiyaTakasaki-shi, Mt. KannonnFujioka-shiKannra-gunn, Shimonita-machi, YashikiIkahoonnsennAgatsuma-gunn, Agatsuma-machiMaebashi-shi, Taguchi-machiHutami-gunn, Funo-sonnTakata-gunn, Yoshida-machiHiba-gunn, Tojo-cho, KushiroFutami-gunn, Huno-muraHiba-gunn, Tojo-cho, TaishakuHiba-gunn, Tojo-cho, MoriwakiTsutsuga-mura, TanojiriHiroshima-shi, Takahi-cho, KamihukawaSaeki-gunn, Yuki-choFutami-gunn, Kisa-choSaeki-gunn, Saeki-choFukuyama-shi, Gobun-choYamagata-gunn, Chiyoda-machiKure-shi, Hiro-machi, KosemariKamo-gunn, Yamato-machiHukayasu-gunn, Kamichika-machiHiba-gunn, Saijo-choFukuyama-shi, Yamano-choFutami-gunn Kisa-choFukuyama-shi, Tsunogo-choFuchu-shi, OguniJinseki-gunn, Sanwa-cho, AgeMiyoshi-shi, UekawadateYmagata-gunn, Geihoku-cho, MiyamaYamagata-gunn, Toyohira-cho, ShijibaraFutami-gunn, Kimita-sonn, MyogadaniAshina-gunn, Shinichi-cho, KawaidaniJinseki-gunn, Yki-choHigashihiroshima-shi, Minaga passSaeki-gunn, Yuki-choYamagata-gun, Toyohira-cho, Nishimune
County, PrefectureGunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Gunnma Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.Hiroshima Pref.
Country JapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapan
Ploidy level
DNA amounts (pg.)
sex/apo apoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapo
Specimen no.Personal TNSMAKno.538838 538836 538835 538951 538834 1112832894612622323728161204949557880 557874 496394 496395 496396 496397 01165694557365 3835557366 3988557368 6704557373 6158557374 4982557376 2788592521 22812571549 571554 438753 450854 450852 450849 450848 478638 478637 451001 451000 35382 11051512179912049507149120494754521204940120493912049381204937444801 444803 444885 445987 445986 445985 444880 815318 1144101204951
Coll. Y. KoikeM. AidaM. AidaY. KimizukaY. KoikeT. MakinoT. MakinoK. SuzukiHJ. ChoiM. HyodoM. HyodoM. HyodoY. SaitoY. SaitoY. SaitoY. SaitoY. YoshimuraS. KobayashiS. KobayashiS. TsutsuiS. TsutsuiS. TsutsuiT.ShibataS. TsutsuiM. YasuA. BabaZ. kanedaZ. kanedaZ. kanedaZ. kanedaZ. kanedaT. OkuboK. HasunumaT. WakuT. WakuN. ImaiT. MakinoN. ImaiK. InukaiT. HagiyamaY. YamamotoY. YamamotoY. YamamotoY. YamamotoN. NimuraN. NimuraN. NimuraK. InukaiK. InukaiH. NagaseN. NimuraH. MiyazakiT. MakinoA. Yamamoto
Year 198119801980197719761909190719662006198619811982198019761976197419861975197519691968196719811982197719801982198019801979197919831993197819791928191019151984198419781979197819811983198319831983198319831983197519061984
Date Aug. 16Nov. 2Oct. 26Nov. 6Jul. 27 Sep. 8Aug. 24Aug. 13Sep. 14Sep. 27Oct. 10Sep. 21Jun. 13Aug. 6Aug. Sep. 4Jul. 20Aug. 24Sep. 15Sep. 1Oct. 15Jun. 14Jun. 27Aug. 21Oct. 3Nov. 2Jul. 20Sep. 5Jul. 23Jul. 23Oct. 9Aug. 16Jul. 8Dec. 6Aug. 12Sep. Sep. 23Aug. 7Sep. 23Aug. 22Jul. 28Sep. 26Oct. 11Sep. 18Oct. 1Oct. 10Nov. 13Nov. 6Oct. 5Jul. 24Sep. 21Aug. 11Aug. 7
Locality Towns, villages, mountains Katori-gunn, Shimousa-machi, NagoyaChoshi-shi, IchibushindennInnba-gunn, Sakae-machiMotono-mura, AranoKatori-gunn, Takomachi, TakodaniMatsudo-shiInzai-machiKashiwa-shiYeongdong-gun, Mt. Cheontae-sanKamiukena-gunn, Yanadani-muraUma-gunn, Doi-machi, Mt. AkahoshiHiyoshi-mura, Mt. JizoOono-gunn, Izumi-muraOono-gunn, Izumi-muraOono-gunn, Izumi-muraOono-shiSakai-gunn, Maruoka-cho, KamitakedaTagawa-gunn, Soeda-machi, Mt. HidehikoTsukiage-gunn, Tsuiki-machi, FusadaBuzenn-shi, Mt. InugatakeFukuoka-shi, Sawara-ku, Mt. SefuriFukuoka-shi, Sawara-ku, Mt, RyoshiiwaTagawa-gunn, Kawara-machi, Ajimi passKitakyushu-shi, Yahatahigashi-ku, Mt. SarakuraIwaki-shi, Mt, FutatsuyaIshikawa-gunn, Asakawa-machiShirakawa-shi, Hatajuku-machiDate-gunn, Ryozan-machiMinamiaizu-gunn, Tadami-maciTamura-gunn, Miharu-machiTamura-gunn, Miharu-machiSukagawa-shi, TanukimoriMinamiaizu-gunn, Tadami-maci, OshimadamuHara-machi, Shintagawa valleyFutaba-gunn, Namie-machiNishishirakawa-gunn, Koseki-muraIwaki-gunn, NakayamaNishishirakawa-gunn, TakisawashiramoriNakatsugawa-shi, KawakamiEna-gunn, Kamiyahagi-cho, Yahagi-riverToki-dhi, Dachi-cho, Hida-riverMashita-gunn, Gero-cho, KadowasashinndennMashita-gunn, Kanayama-cho, OkukanayamaKamo-gunn, Shirakawa-cho, KiriiGujo-gunn, Hachiman-cho, OnabiMashita-gunn, Hagiwara-cho, NishiuedaMugi-gunn, Mugi-machi, Nakanoho, Hokujo passEna-shi, KusumiNakatsugawa-shi, AgiYoshiki-gunn, Ko-machi, ImamuraGujo-gunn, Wara-cho, KakuraEna-shi, TakenamiIbi-gunn, Kasuga-mura, FuruyaUsui-gunn, Matsuida-cho, Uramyogi
1. Continued.peNdix DNA Ploidy amounts County, levelCountry(pg.)PrefectureJapanChiba Pref.JapanChiba Pref.JapanChiba Pref.JapanChiba Pref.JapanChiba Pref.JapanChiba Pref.JapanChiba Pref.JapanChiba Pref.KoreaChungcheongbuk-doJapanEhime Pref.JapanEhime Pref.JapanEhime Pref.JapanFukui Pref.JapanFukui Pref.JapanFukui Pref.JapanFukui Pref.JapanFukui Pref.JapanFukuoka Pref.JapanFukuoka Pref.JapanFukuoka Pref.JapanFukuoka Pref.JapanFukuoka Pref.JapanFukuoka Pref.JapanFukuoka Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanFukushima Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGifu Pref.JapanGunnma Pref.
ap sex/apo apoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapo
NII-Electronic Library Service
The Japanese Society for Plant Systematics
February 2015 Hori & al.—Sexual type of Dryopteris chinensis 43
Specimen no.Personal TNSMAKno.538127 538128 538131 77592448398 448394 448416 448397 449920 1207585589577 512548 512550 512545 512219 512215 512214 512225 512380 512381 512540 512544 512543 410317Dpaci 813592049 324461207593592040 33334592043 33411592046 1282592048 3286612049521212049481204934120493312049321204931712208 126635 421326 421329 421328 421327 420701 421314 421323 421322 421320 421310 290443378490341863391349296045386036
Coll. N. YoshikawaY. KimizukaT. MadokoroY. ArakiS. YamauchiK. OhoraY. HiguchiS. YamauchiS. YamauchiN. SakawaO. AsanoK. ShojiO. AsanoK. ShojiY. UenoY. UenoO. AsanoM. TakeichiK. TakahashiK. TakahashiK. TakahashiK. ShojiK. ShojiK. HoriT. MinamitaniT. KariyazakiT. MinamitaniT. MinamitaniT. MinamitaniT. MinamitaniM. MinemuraY. KimizukaM. MinemuraH. TsutsumiH. TsutsumiH. OkubaraH. TsutsumiM. MatsumuraH. TsutsumiH. TsutsumiH. TsutsumiH. TsutsumiH. TsutsumiH. TsutsumiH. TsutsumiH. TsutsumiH. TsutsumiH. TsutsumiT. MakinoK. AsanoK. AsanoA. YamamotoA. YamamotoK. Asano
Year 197719821982193519801981196219801983197919831982198219821975197519821982197219721974198119822013198219781982198219681982198819861959198219821981198119551981198119811981198119811981198119811981193019911993200219381992
Date Sep. 15Jul. 3Aug. 13 Sep. 9Jul. 26Aug. 18Nov. 24Nov. 2Aug. 25Oct. 22Sep. 23Sep. 5Jul. 10Aug. 14Aug. 24Aug. 7Jul. 10Jul. 12Sep. 23Aug. 25Aug. 13Aug. 4Jun. 18Sep. 5Jul. 24Oct. 16Oct. 15Sep. 22Sep. 20Aug. 22Sep. 10Aug. 18Oct. 17Aug. 5Jul. 22Jul. 23Aug. 22Aug. 29Sep. 24Aug. 25Oct. 10Sep. 23Aug. 24Aug. 26Oct. 27Aug. 22Oct. 22Jul. Aug. 6Nov. 14Jun. 11Aug. 10Apr. 2
Locality Towns, villages, mountains Kyoto-shi, Kita-ku, HankokudakayamaSakyo-ku, Hirogawarasyakuya-choHiyoshi-machi, KisumiKitaguwada-gunn, Hosono-muraNabari-shi, KamimitaniKumano-shi, Asuka-choIsshi-gunn, Misugi-muraUreshino-cho, HikawaMisato-mura, HinataKurokawa-gunn, Taiwa-cho, KozurusawaNaruse-cho, MiyatotsukihamaShibata-choSendai-shi, Mt. DainenjiNatori-shi, TategoshiTajiri-cho, Mt. KagoboKanann-cho, Wabuchi-yamaNaruse-cho, Ushiami pondKurihara-gunn, Kurikoma-choMatsushima-cho, Mt. AtagoIshinomaki-shi, TashirojimaOga-machi, Mt. KinkaMiyagi-machi, KumaganeSndaishi, Mt. SaboNobeokashi, Kitagawa-cho, KawachimyoHigashiusuki-gunn, Togo-cho, ChigoaraiKoyu-gunnNishiusuki-gunn, Takachiho-machi, TorokuNishiusuki-gunn, Hinoakge-machi, OikawaHigashiusuki-gunn, Saigo-mura, Mt. ShimizudakeKoyu-gunn, Tono-machi, SeiyabaraNagano-shi,HoshinaKaruizawa-machi, Mt. HanareNagano-shi, shinko-machi, HinotaNeba-mura, HibaraSakaki-machi, ShinchiMatsumoto-shiIida-shi, Chiyono pondMiho-muraFukushima-cho, ChigonoSaku-shi, Uchiyama passMinamikiso-machi, SakamotodairaAnan-cho, MinamisawaSaku-machi, YojiOguwa-muraKamimatsu-choOguwa-muraNaganao-shi, MurayamaNeba-muraOmachi-shiShimoina-gunn, Takamori-choShimoina-gunn, Matsukawa-cho, SeisnchiIida-shi, Tatsuoka, Suzuoka parkKaruizawa-machi, Mt. HanareShimoina-gunn, Tenryuu-mura, Shimizu
County, PrefectureKyoto-fu.Kyoto-fu.Kyoto-fu.Kyoto-fu.Mie Pref.Mie Pref.Mie Pref.Mie Pref.Mie Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyagi Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Miyazaki Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.Nagano Pref.
Country JapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapan
Ploidy level 3x
DNA amounts (pg.) 19.5
sex/apo apoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapo
Specimen no.Personal TNSMAKno.430818 521140 521143 35383 458094 458097 458095 27107528024 528025 528026 528090 528104 528105 528021 528022 01162677110518552474 338086176997 176810 558446 558447 558448 558438 558435 558443 558357 558358 558429 123660 151224 815322 519816 519821 519817 23350 493539 493540 493537 493528 493531 335706451253 451562 451561 451563 144515574045 146151204984 85301204983 130721204982 6478538126
Coll. M. MatsumuraT. TakedaT. TakedaZ. TashiroT. ShiraiwaT. ShiraiwaT. ShiraiwaK. IwasakiY. nakagiY. nakagiY. nakagiY. KoikeM. yasuM. TanayaM. yasuM. yasuN.NakatoT. MakinoS. KomakiJ. HayashiM.KikuchiM.KikuchiT. WakuT. WakuM. SuzukiK. TakahashiZ. kanedaY. OdaM. SuzukiM. NeichiK. InoueH. OkuyamaChung In-ChoK.YaoS. MitaniS. MitaniS. MitaniZ. TashiroA. YamamotoY. KobayashiE. FukushiK. TanakaN. IidaT. NakaikeY. YamamotoK. YamaokaT. IwanoT. MiyazakiT. MakinoS. TsutsuiJ. BeppuM. ShirotoM. ShirotoN. Yoshikawa
Year 198019751978192819651966197619541972197419791979197819771979197419871923197819961967196719801977198019801980198119791982196919501947198319931975197619161981197919821982196219671966197819801979188719771978197719641981
Date Sep. 15Aug. 3Sep. 4Jul. 20Aug. 21Aug. 10Oct. 25Oct. 24Aug. 20Oct. 5Jul. 27Aug. 19Jul. 29Oct. 17Jul. 29Jul. 21Jul. 29 Oct. 12Sep. 8Sep. 29Sep. 28Jul. 25Sep. 17Aug. 12Jul. 28Jul. 28Oct. 11Aug. 22Sep. 18Aug. 5Aug. 1Aug. 30Sep. 30Oct. 18Jun. 27Sep. 5Jul. 24Oct. 10Aug. 5Jul. 10Sep. 5Aug. 10Jun. 14Nov. 20Aug. 28Aug. 23Jul. 28Aug. 17Aug. 13Aug. 6Sep. 11Nov. 15Jul. 26
Locality Towns, villages, mountains Saeki-gunn, Okimi-choJinnseki-gunn, Jinseki-cho, NoyaHiroshima-shi, Kabe-machiMt. FukuchimuroShiso-gunn, Hagacho, OnzuiAsako-gunn, Itako-cho, TochiharaTaki-gunn, Sasayama-cho, HiuchiwanMt. KoganeKuji-gunn, Satomi-mura, OkamiHitachiota-shi, Inagi-choMt. TsukubaMt. TsukubaKuji-gunn, Mt. NabeashiNamegata-gunn, Ushibori-cho, NagayamaDaigo-cho, Mt. HanagameTakahagi-shi, TashiroKitaibaraki-shi, AzukibataMt. TsukubaHosu-gunn, Anamizu-machi, NonamiKaga-shi, TsurugatakiRaga, Tanohata-muraNaraiga,TonohatamuraSennmaya-cho, Nagahashi-ShirabataMiyako-shi, JodogahamaIsawa-cho, Minamitsuda, KeshozakaKamaishi-shi, ShimoarakawaOhunato-shi, YonesakishindennShimohei-gunn, Tanohata-mura, KiriushiIchinoseki-shi, Hanaizumi-cho, KazawaShimohei-gunn, Iwaizumi-ch0, AkkaRikuzentakata-shi, Hirota-choMiyako-shi Lushan, Da Yue Shan, Kawaga-gunn, Shionoe-cho, KaminishiNakatado-gunn, Kotonan-cho, MyojinKida-gunn, Miki-machiMt. KaimonKawasaki-shi, Takatsu-kuTsukui-gunn, Tsukui-cho, NishinonoYokohama-shi, Kanagawaku, NishiteraoOdawara-shi, HunabaraHakoneKasaki-shi, Aso-kuNagaoka-gunn, Ootoyo-cho, KajigamoriTakaoka-gunn, Higashitsuno-mura, ShindennUmaji-mura, YanaseAgawa-gunn, Ikegawa-cho, Yasui valleyTakaoka-gunn, Sakawa-machiAso-gunn, Oguni-machi, OkuyamaAso-gunn, Oguni-machi, SeiwaMinamata-shi, Mt. YajiroMinamata-shi, KukinookawaAmata-gunn, Miwa-cho, Ubara
1. Continued.peNdix DNA Ploidy amounts County, levelCountry(pg.)PrefectureJapanHiroshima Pref.JapanHiroshima Pref.JapanHiroshima Pref.JapanHyogo Pref. JapanHyogo Pref.JapanHyogo Pref.JapanHyogo Pref.JapanHyogo Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIbaraki Pref.JapanIshikawa Pref.JapanIshikawa Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.JapanIwate Pref.KoreaJeju-do.ChinaJiangxi prov.JapanKagawa Pref.JapanKagawa Pref.JapanKagawa Pref.JapanKagoshima Pref.JapanKanagawa Pref.JapanKanagawa Pref.JapanKanagawa Pref.JapanKanagawa Pref.JapanKanagawa Pref.JapanKanagawa Pref.JapanKochi Pref.JapanKochi Pref.JapanKochi Pref.JapanKochi Pref.JapanKochi Pref.JapanKumamoto Pref.JapanKumamoto Pref.JapanKumamoto Pref.JapanKumamoto Pref.JapanKyoto-fu.
ap sex/apo apoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapo
NII-Electronic Library Service
The Japanese Society for Plant Systematics
44 Acta Phytotax. Geobot. Vol. 66
Specimen no.Personal TNSMAKno.01139464011394540113944701139446011394270113924601139425011394090113940301139394156159 675366 675867 676562 0102627601026277010262750102626801026265010262550102625101026248010262290102622701026221538328 446859 739143 010263080102629601026285262834312865262333443304 101543 213923 270091443779 445064 445063 448663 1204941 445204 445205 893709 820702 820230 1204956 1204955 510638 510367 510636 510361
Coll. T. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataM. SatakeTetsuya kawasakiTetsuya kawasakiTetsuya kawasakiT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataT. IwataM. ShimosegawaS. OjimaT. YamadaT. IwataT. IwataT. IwataK. SuzukiA. YamamotoK. SuzukiT. WakuT. HashimotoS.OkamotoK. SuzukiM. MatsumuraH. YamamotoH. YamamotoGakuson ImadaT. HosokuraT. SatoT. HosokuraH. OkuyamaY. SakumaY. SakumaH. SuzukiH. SuzukiA. SuzukiT. SatoH. SuzukiI. Yamashita
Year 200019971996199619931993199319881990199119611968196819461984198419841984198419881985198519871987198719831983199219711982198319701938196719771944197719751976197719781980198719831983194319791979198219821977198019821972
Date Nov. 8Jul. 19Oct. 18Oct. 24Oct. 31Oct. 11Sep. 25Oct. 9Jul. 11Jul. 31Oct. 15Oct. 10Oct. 26Sep. 8Jul. 23Jul. 23Aug. 15Oct. 19Nov. 10Aug. 31Nov. 1Nov. 1Aug. 23Sep. 1Nov. 17Oct. 29Nov. 14Aug. 30Jul. 4Sep. 13Aug. 7Nov. Sep. 18Jun. 25Sep. 24Nov. 7Jan. 10Sep. 7Oct. 17Aug. 27Aug. 3Aug. 10Jul. 31Sep. 18Sep. 19Nov. 19Sep. 4Aug. 8Jul. 30Jul. 30Oct. 23Oct. 15Sep. 7Sep. 24
Locality Towns, villages, mountains Hannou-shi, Mt. TenrannOgose-machi, Mt. OtakadoriYoshida-machi, Mt. ShiromineMinano-machiChichibu-shi, UrayamaOgano-machi, Ushikubi passRyokami-mura, Mt. RyokamiNagatoro-machi, Otsuki passOtaki-mura, MisobaArakawa-mura, SaginosuSayama hillUrawa-shi, DaimonUrawa-shi, OyaguchiIruma-gunn, Hidaka-choNagatoro-machi, Mt. IwaneNagatoro-machi, Mt. IwaneNagatoro-machi, Mt. JinmiNaguri-mura, HitomiiriHannou-shi, KoshuNagatoro-machi, HuubuYorii-machi, KamabuseYorii-machi, KamabuseYokoza-machi, IkukawaChichibu-shi, OkaguraChichibu-shi, TochiyaOsato-mura, YoshiokaKamiizumi-mura, MukoudairaSyomaru passHiki-gunn, Ogose-machi, Mt. KuroChichibu-gunn, Ykoze-mura, Mt. FutagoChichibu-shi, TsunakubotaniIruma-shi, Ogose-machi, Mt. KuroIruma-gunn, Mt. MonomiHannno-shiShiga-gunn, Shiga-machi, MinamihiraMt. HiraKouga-gunn, Kouka-cho, AburahiOtsu-shi, Mt. HieiOchi-gunn, Mizuho-choIzumo-shi, TatsukuekyoIishi-gunn, Tonnbara-co, Mt. KotohikiOta-shi, Mt. Sannbe, Himenoga pondOhito-cho, ChojabaraFujinomiya-shiGotenba-shi, InnnoMt. AmagiFujinomiya-shi, Mt. KenashiFujinomiya-shi, Mt. KenashiFuji-shi, MaruhiFuji-shi, MaruhiAmagiyogashima-cho, Nekogoe passHamakita-shi, MiyaguchiShizoka-shi, IkawaIwata-gunn, Sakuma-machi, Yoshizawa
County, PrefectureSaitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Saitama Pref.Shiga Pref.Shiga Pref.Shiga Pref.Shiga Pref.Shimane Pref.Shimane Pref.Shimane Pref.Shimane Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.Shizuoka Pref.
Country JapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapanJapan
Ploidy level
DNA amounts (pg.)
sex/apo apoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapo
Specimen no.Personal TNSMAKno.574885 573446 2805573451 17376511746 511749 511751 596930 596931 120495312049811204954FOP-P48941204979FOP-P62271204980FOP-P19401204977FOP-P17121204945120494201092536011616530116165601161652523310 523331 523333 334550 334517 523339 523298 523303 523304 523328 523329 523332 112929527959 500705 501292 501291 501289 448013 6149544903 267119448035 448036 442541 935761 935772 935773 935771 935770 935780 935774 935785 0113945101139469
Coll. B. MatsumuraS. KobayashiS. TsutsuiY. InoueY. TsujimotoY. TsujimotoT. TakahashiT. TakahashiN. TanakaM. HadnoY. TakaokaM. ArakaneY. TakaokaY. TakaokaY. ObataY. ObataI. AkasawaY. ObataY. ObataY. ObataT. WatanabeK. TakayamaY. ObataT. WatanabeT. WatanabeT. SaitoT. SaitoY. ObataY. ObataY. ObataY. ObataY. ObataT. MakinoA. KomakiM. SdatamatsuY. KuranariY. KuranariY. KuranariS. TsutsuiM. IwamuraK. SuzukiM. ShimosegawaM. ShimosegawaM. ShimosegawaS. KobayashiM. ShimosegawaS. KobayashiM. ShimosegawaS. KobayashiT. WakuM. ShimosegawaK. MashitaT. IwataT. Iwata
Year 197419721979197619771981198119821988195219821973197819781985198419651982198219821976197619801975197519801979198119811982198019801932198119791976197519751968197819731981198219831982198219821982198219801982198019662006
Date Aug. 14Sep. 24Jul. 19Aug. 16Aug. 21Sep. 6Jul. 11Aug. 28Jul. 7Aug.Aug. 7Sep. 24Aug. 3Aug. 18Aug. 21Aug. 24Aug. 17Nov. 9Sep. 16Oct. 9Sep. 18Aug. 25Oct. 27Nov. 5Sep. 17Nov. 2Sep. 9Nov. 13Nov. 12Jun. 27Oct. 27Oct. 27Aug. 30Sep. 27Aug. 19Jul. 17Nov. 8Jul. 23Sep. 1Aug. 18Aug. 24Nov. 21Nov. 13Jun. 12Nov. 21Sep. 20Nov. 21Aug. 17Oct. 9Jul. 12Nov. 8Jul. 13Sep. 8Oct. 3
Locality Towns, villages, mountains Tsushima-shi, uehara-machi, Mt. MitakeTsushima-shi, shimoagata-gunn, Mitsushima-choTsushima-shi, Kamiagata-machi, Mt. MitakeGosho-shi, KongousannNara-shi, Minamisho-machiSakurai-shiKamoshi, IwanoAomi-cho, UejiSado-gunn, Aikawa-cho, IwayaguchiKusu-gunn, Kusu-machi, YamauraShimoge-gunn, Yamakuni-machiKusu-gun, Kusu-machi, Mt. HaneUsa-shi, Mt. OmotoNishikunisaki-gunn, Matama-machiTsuyama-shi, HigashiichinomiyaAida-gunn, Nishiawakura-mura, Mt. KijiKawakami-gunn, Bicchu-machiIhara-shi, Kinogo-machiAida-gunn, Mimasaka-choJobo-gunn, Ukann-cho, KamiukannAtetsu-gunn, Osa-cho, KamiosakabeKatsuta-gunn, Shoboku-cho, YamagataJobo-gunn, Kayo-cho, YoshikawaAtetsu-gunn, Tetsuta-choKawakami-gunn, Nariwa-cho, SakamotoMitsu-gunn, Takebe-choOiwaTsukubo-gunn, Kiyone-sonn, KarubeKume-gunn, Kumenan-cho, YugeNiimi-shi, MiyanohanaKayo-cho, OiwaKyo-cho, JinnbaraKibi-gunn, Syowa-machiToyono-gunn, Noza-machi, ShimodaSaga-gunn, Fuji-machi, Mt. IbaraHigashimatsura-gunn, Nananayama-muraHigashimatsura-gunn, Ouchi-choImari-shi, Higashiyamashiro-machiKannzaki-gunn, Higashisefuri-muraNishimatsura-gunn, Nishiarita-machiMt. KaminariOmiya-shi, KiyokawaderaHasuda-shi, MagomeSayama-shi, KashiwabaraChichibu-gunn, Ryokami-muraChichibu-gunn, Higashichichibu-muraOgawa-machiOtaki-mura, NakasouriHannou-shi, IwabuchiUrawashi, MisonoSiraoka-machi, MinamishinjukuHonjo-shi, OkuboyamaOtaki-mura, Mt. MitakeRyokami-mura
1. Continued.peNdix DNA Ploidy amounts County, levelCountry(pg.)PrefectureJapanNagasaki Pref.JapanNagasaki Pref.JapanNagasaki Pref.JapanNara Pref.JapanNara Pref.JapanNara Pref.JapanNiigata Pref.JapanNiigata Pref.JapanNiigata Pref.JapanOita Pref.JapanOita Pref.JapanOita Pref.JapanOita Pref.JapanOita Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOkayama Pref.JapanOsaka-fu.JapanSaga Pref.JapanSaga Pref.JapanSaga Pref.JapanSaga Pref.JapanSaga Pref.JapanSaga Pref.JapanSaga Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.JapanSaitama Pref.
ap sex/apo apoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapoapo
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