Genetic Analysis of Elite Genotype of Aromatic Rice

131 Pages·2012·1.65 MB·English

Genetic Analysis of Elite Genotype of
Aromatic Rice

THESIS

Submitted to the

Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur

In partial fulfilment of the requirements for
the Degree of

MASTER OF SCIENCE

In

AGRICULTURE

(GENETICS AND PLANT BREEDING)

By

MOHINI RAMESH WANKHADE

Department of Plant Breeding and Genetics
College of Agriculture, Jabalpur 482004
Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, MP
2014
CERTIFICATE – I
This is to certify that the thesis entitled “Genetic Analysis of Elite
Genotype of Aromatic Rice” submitted in partial fulfilment of the requirements for
the degree of “MASTER OF SCIENCE IN AGRICULTURE” in Department of
Plant Breeding and Genetics of Jawaharlal Nehru Krishi Vishwa Vidyalaya,
Jabalpur (M.P.) is a record of the bonafide research work carried out by Miss
Mohini Wankhade under my guidance and supervision. The subject of the thesis
has been approved by the Student’s Advisory Committee and the Director of
Instructions.
All the assistance and help received during the course of the investigation
has been dully acknowledged by her.

Signature
Place : Jabalpur (Dr. G.K. Koutu)
Chairman of the Advisory Committee
Date :

THESIS APPROVED BY THE STUDENT’S ADVISORY COMMITTEE

Committee Name Signature
Chairman Dr. G.K. Koutu ....................................
Member Dr. D.K. Mishra ....................................
Member Dr. Y. Singh ....................................
Member Dr. R.B. Singh ………………………...
CERTIFICATE - II

This is to certify that the thesis entitled, “Genetics Analysis of Elite
Genotype of Aromatic Rice" submitted by Ms. Mohini Ramesh Wankhade to
the Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, in partial fulfilment of
the requirements for the degree of MASTER OF SCIENCE in AGRICULTURE
in the Department of Plant Breeding and Genetics, has been, after evaluation,
approved by the External Examiner and by the Student’s Advisory Committee
after an oral examination of the same.

Place : Jabalpur (Dr. G.K. Koutu)
Chairman of the Advisory Committee
Date :

THESIS APPROVED BY THE STUDENT’S ADVISORY COMMITTEE

Committee Name Signature
Chairman Dr. G.K. Koutu ....................................
Member Dr. D.K. Mishra ....................................
Member Dr. Y. Singh ....................................
Member Dr. R.B. Singh ………………………...
Head of the Department Dr. D.K. Mishra ........................
Director of Instruction Dr. S.K. Shrivastava ........................

Declaration and undertaking by the candidate
I, Mohini Ramesh Wankhade D/o Shri R.D. Wankhade Certify the work
embodied in thesis entitled “Genetic Analysis of Elite Genotype of Aromatic
Rice" is my own first hand bonafide work carried out by me under the guidance
of Dr. D. Khare at Department of Plant Breeding and Genetics, Jawaharlal Nehru
Krishi Vishwa Vidyalaya, College of Agriculture, Jabalpur during 2013-14.
The matter embodied in the thesis has not been submitted for award of
any other degree / diploma. Due credit has been made to all the assistance and
help.
I, undertake the complete responsibility that any act of misinterpretation,
mistakes, and errors of fact are entirely of my own.
I, also abide myself with the decision taken by my advisor for the
publication of material extracted from the thesis work and subsequent
improvement, on mutually beneficial basis, provided the due credit is given,
thereof.

Place:
Date: MOHINI RAMESH WANKHADE
ACKNOWLEDGEMENT
I bow my head with great reverence before God, whose ideologies
have always been an inspiring source in my life and has enabled me to
achieve this seemingly invinciable task.
It is a matter of great jubilance and privilege to express my deepest
sense of indebtness and reverence to venerable Dr. G.K. Koutu, Principal
Scientist, Department of Plant Breeding and Genetics, chairman of my
advisory committee and officer I/C Breeder Seed Production Unit (F.C.),
Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, for his precious
guidance and keen interest during the course of study and preparation of
manuscript. I am highly thankful to him for keeping my moral high, being
attentive and providing valuable suggestions. Without his help it would not
have been possible to complete this bothersome work of investigation.
I am deeply obliged to all the members of my advisory committee
namely, Dr. D.K. Mishra Director of Farms, Professor and Head,
Department of Plant Breeding and Genetics, Dr. Yogendra Singh senior
scientist, Department of Molecular Biology and Biotechnology and
Dr. R.B. Singh, Department of Mathematics & Statistics, JNKVV, Jabalpur
for their valuable guidance and timely help during the course of this
investigation.
I wish to express deep sense of gratitude to the honorable Vice-
Chancellor Dr. V.S. Tomar, Dr. S. S. Tomar, Director Research Services,
Dr. S.K. Rao, Dean Faculty of Agriculture, Dr. S.K. Shrivastava, Director of
instruction and Dr. R. V. Singh, Dean, College of Agriculture, J.N.K.V.V.,
Jabalpur for providing necessary facilities during the course of
investigation.
I express my warmest feelings with deep sense of gratitude and
regards to all respected teachers of my department Dr. P.K. Moitra,
Dr. A.K. Mehta, Dr. Dhirendra Khare, Dr. A.N. Shrivastava, Prof. V.K.
Gour, Dr. R.S. Shukla, Dr. R.K. Dubey, Dr. S.K. Bilaiya, Dr. M.K.
Shrivastava, Dr. (Mrs.) Anita Babbar, and Sunita Pandey without whose
benevolent guidance and constant motivation it would not have been
possible to complete this project.
I express my sincere gratitude and special thanks to Dr. S.K. Singh,
Scientist, Department of Plant Breeding and Genetics, and Dr. S.S.Shukla,
for his valuable suggestions and healthy critism during the course of
investigations.
I am deeply obliged to Shri Kishori, Shri Manoj, Shri Brijesh and Shri
Rammu of Rice Improvement Project, Seed Breeding Farm, JNKVV,
Jabalpur for the help rendered to carry out the experiment.
I would like to mention and express my special thanks to Dr. Sharad
Tiwari, Head of the Department, Molecular Biology and Biotechnology,
Dr. Ashish Gupta (Department of Plant Pathology, Rewa), Dr. Priya Nair,
Mrs. Meghna and Vijay prakash Bansal for rendering me support and
helping me in my work.
I express my sincere thanks to my seniors Arpita Shrivastava,
Niharika Shukla, Monika Singh, Mrs. Neha Sohegaura, Namita Pal, Swati
Sharma, and Meenakshi Patil. My friends – Punam, Priya, Jyoti, Kandla,
Sheetal, Shahnaz, Ashita, Neelam, Chaitali, Shamma, Meena, Abhijeet,
Abhishek, Jitendra, Mukesh, Pranay, Lokesh, Santosh, Rajkumar, Ajay,
Nikhil, all juniors and Yadav computer’s bhaiya.
I have no words to express my feelings of humble gratitude for my
lovely parents Mr. Ramesh Wankhade and Mrs. Aasha R. Wankhade and
my younger sweet sister Shivani and brother Mahesh dada and Gaurav
whose cheerful presence and contact filled with joy and energy, was of
great value in my study as well as throughout my life.
Lastly I would like to convey my cordial thanks to all those unmentioned
persons who helped me to fulfill my dream, come true.
Finally, I am thankful to Almighty God for his heavenly blessing
which has enabled me to achieve this seemingly dauntless task.

Place : Jabalpur
Date : July, 2014 (Mohini Ramesh Wankhade)
Copyright ©Jawaharlal Nehru Krishi Vishwa Vidyalaya,
Jabalpur Madhya Pradesh 2014
Copyright Transfer Certificate
Title of the Thesis – Genetic Analysis of Elite Genotype of Aromatic Rice.
Name of the candidate – Mohini Ramesh Wankhade
Subject – Genetics and Plant Breeding
Department – Plant Breeding and Genetics
College – College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya,
Jabalpur, Madhya Pradesh
Year or thesis submission –2014

Copyright Transfer

The undersigned Mohini Ramesh Wankhade assigns to the Jawaharlal
Nehru Krishi Vishwa Vidyalaya, Jabalpur Madhya Pradesh, all right under
Copyright Act, that may exists in and for the thesis entitled “Genetic Analysis
of Elite Genotype of Aromatic Rice” submitted for the award of M.Sc.(Ag.)
Genetics and Plant Breeding.

Date:
Place: Jabalpur

Dr. G. K. Koutu (Mohini Ramesh Wankhade)
(Major Advisor) (Student)
LIST OF CONTENTS

Chapter Title Page
1. Introduction 1-3
2. Review of literature 4-21
3. Material and Methods 22-46
4. Result 47-84
5. Discussion 86-99
6. Summary, Conclusion and Suggestions for 100-103
further work
6.1 Summary 100-101
6.2 Conclusions 101-102
6.3 Suggestions for further work 102-103
7. Bibliography 104-111
8. Appendices I – II
Curriculum vitae
LIST OF TABLES
Table Page
Title
no. No.
3.1 List of Genotypes used in study 23
3.2 ANOVA table for Randomized Block Design 32
3.3 Details of selected genotypes included for 38
molecular analysis
3.4 Composition of DNA extraction buffer (100ml) 40
3.5 List of sequencws of ten SSR markers 44
3.6 Reaction mixture for PCR to detect SSR markers 45
3.7 Temperature profile used in PCR Amplification for 45
SSR
4.1 Frequency Distribution of Morphological 48-49
Characters in Rice Genotypes
4.2 Analysis of variance for yield, yield and quality 59
attributing traits in rice genotypes
4.3 Parameters of genetic variability for yield and 61
quality attributing traits in rice genotypes
4.3.1 Heritability estimates along with Genetic advance 65
4.4 Estimates of phenotypic correlation coefficient for 69-70
various yield and quality attributing traits
4.5 Estimates of genotypic path coefficient (direct and 76-77
indirect effects) for various yield and quality
attributing traits on grain yield per plant
4.6 Markers used with number of allel, polymorphic 82
and unique alleles and allele size
4.7 Markers used along with the gene diversity,major 83
allele frequency, heterozygosity and PIC values
4.8 Markers amplified unique alleles, their size and 83
genotypes
LIST OF FIGURES

Number Title Page
No.
1 Frequency distribution for the characters like viz., 52
basal leaf colour, leaf pubescenceof blade surface
and leaf auricle
2 Frequency distribution for the characters like viz., 53
auricle colour, ligule colour and shape of legule
3 Frequency distribution for the characters like viz., 54
flag leaf attitude of blade, spikelet colour of stigma
and stem anthocyanin colouration of node
4 Frequency distribution for the characters like viz., 55
spikelet density of pubescence, sterile lemma
colour and spikelet colour tip of lemma
5 Frequency distribution for the characters like viz., 56
panicle exsertion, panicle attitude of branches and
panicle awn
6 Frequency distribution for the characters like viz., 57
panicle distribution of awn and panicle colour of
awn
7 Path diagram showing direct and indirect effect on 80
dependent variables
8 Dendogram showing the clustering pattern of 84-85
various aromatic rice genotypes based on SSR
markers