Generation and characterisation of monoclonal antibodies against cell cycle and cytokinesis ...

308 Pages·2013·6.35 MB·English

by  Cristina Costa

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a Universidade Nova de Lisboa
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o Generation and characterisation of monoclonal antibodies against
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n cell cycle and cytokinesis regulators in Trypanosoma brucei
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aierroC tsniaga 3102 Cristina Isabel Corre ia de Almeida Costa
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o DISSERTAÇÃO PARA A OBTENÇÃO DO GRAU DE DOUTOR EM
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it CIÊNCIAS BIOMÉDICAS, ESPECIALIDADE DE PARASITOLOGIA
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Universidade Nova de Lisboa

Instituto de Higiene e Medicina Tropical

Thesis: Generation and characterisation of monoclonal antibodies against cell cycle and
cytokinesis regulators in Trypanosoma brucei

Author: Cristina Isabel Correia de Almeida Costa

Supervisor: Doctor Carlos Novo, IHMT, UNL

Co-supervisor: Professor Tansy C. Hammarton (University of Glasgow)

Tutorial Committee:
Doctor Carlos Novo (IHMT, UNL)
Doctor Luís Távora Tavira (IHMT, UNL)
Professor Virgílio Estólio do Rosário (IHMT, UNL)

Dissertation presented to obtain a PhD degree in Biomedical Sciences, speciality of
Parasitology, under the supervision of Doctor Carlos Novo, Professor Tansy C.
Hammarton and Professor Virgílio Estólio do Rosário. Financial support was provided
by Fundação para a Ciência e Tecnologia (FCT) with a PhD fellowship Grant (SFRH /
BD / 40223 / 2007).

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Universidade Nova de Lisboa

Instituto de Higiene e Medicina Tropical

Título da tese: Produção e caracterização de anticorpos monoclonais contra reguladores
do ciclo celular e da citocinese em Trypanosoma brucei

Autor: Cristina Isabel Correia de Almeida Costa

Orientador: Investigador Doutor Carlos Novo, IHMT, UNL

Co-orientador: Professora Doutora Tansy C. Hammarton (University of Glasgow)

Comissão Tutorial:
Investigador Doutor Carlos Novo (IHMT, UNL)
Investigador Doutor Luís Távora Tavira (IHMT, UNL)
Professor Doutor Virgílio Estólio do Rosário (IHMT, UNL)

Dissertação apresentada para cumprimento dos requisitos necessários à obtenção do
grau de Doutor em Ciências Biomédicas, especialidade de Parasitologia, realizada sob a
orientação científica do Investigador Doutor Carlos Novo, da Professora Doutora Tansy
C. Hammarton Professor e do Professor Doutor Virgílio Estólio do Rosário. Apoio
financeiro da FCT com a bolsa de doutoramento SFRH / BD / 40223 / 2007 no âmbito
do III Quadro Comunitário de Apoio.

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Dedico esta tese,

À minha irmã Ana Isabel que comigo construiu um castelo
À minha mãe, minha estrela-guia, que me tornou na mulher que sou hoje
Ao meu querido avô, que tanta saudade deixa, que me ensinou a ser “Guerreira”

“…Ser feliz é reconhecer que vale a pena viver
Apesar de todos os desafios, incompreensões e períodos de crise.
Ser feliz é deixar de ser vítima dos problemas e
Se tornar um autor da própria história.
É atravessar desertos fora de si, mas ser capaz de encontrar
Um oásis no recôndito da sua alma...
É agradecer a Deus a cada manhã pelo milagre da vida.
Ser feliz é não ter medo dos próprios sentimentos.
É saber falar de si mesmo.
É ter coragem para ouvir um 'não'.
É ter segurança para receber uma crítica, mesmo que injusta.

Pedras no caminho?
Guardo todas, um dia vou construir um castelo...”
(Fernando Pessoa)

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ACKNOWLEDGEMENTS

A lot of people have been by my side on this amazing adventure.
First of all, I would like to thank my supervisor, Dr Carlos Novo, for all the trust
he put on me, for the guidance and support, and especially for always having my back
and helping me when I was in need.
I would also like to thank my co-supervisor Prof. Tansy Hammarton, who
allowed me to work in her lab and taught me so many things I would not know where to
start. Thank you for the support, the confidance and for helping me “grow” in the lab.
This project allowed me to meet amazing people, whether in former UTPAM:
Tiago, Bé, Ângela, Maria, Carla, Sofia, Ana Armada, Ana Domingos and Fernando; and
recently in IHMT: Pimpolho, Cátia, Isabel, Pedro, Ana, Idalécia and so many other
people. To all of you, thanks for the support and for accompanying me on this journey.
During my PhD I travelled regularly to Glasgow and a part of me will always
stay there. First of all I would like to thank my “brother” Dave (one of my Musketeers).
He turned Glasgow into a second home, his friendship helped me through the bad
moments, but most important: he always had a HUG and a SMILE to share. To my two
other Musketeers: Will and Nath, our adventures shall be immortal. To my sweet Craig,
for caring and giving me love; to Elaine, for the fun and friendship; to Cat, for being my
shoulder on the last year; to Jimbo, for the fun and the boozy lunches. To the brasilian
gang: Herbert (my dear friend Safado), Tatiana, Dani, thank you for the the crazy times.
To the Hammarton group: Corinna, Sophie, Glynn, Elizabeth, thanks for the help
around the lab and for making work days such a blast. To everyone else (Jeremy,
Elmarie, Esther, Amy, Ben, Fiona, Kirsty, Eileen…), it has been a pleasure to share
such great moments and unforgettable experiences. I hope to see you all one day again,
on a Friday afternoon at 4 p.m. in the usual place: Tennents!!
Although everyone was important for this journey, two special people have
walked alongside me, suffered my drawbacks and enjoyed my victories: my Marias. To
Maria Sandra: thanks for always being there for me and for always caring. You are an
amazing person with a giant heart, and I am blessed to call you a friend. Having you,
Carqueijo and Mini-Spartacus in my life has lightened it up. To my other Maria, Maria
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Raquel, this would not have been possible without you. You became my “sister”, and I
shall never forget that you always saw the best in me. Thank you for always being there,
for supporting me through the Westerns of Doom, for the crazy adventures, and for
letting me be a part of your life outside the lab where I met Zé and Mini-Tarzan. Still
today, when I am sad, I grab a glass of wine, start cooking and a voice starts
singing…”You are the dancing queen….”.
To my family: thank you for believing in me. To my mother and father: without
your faith in me I would not have successfully finished this project. To my guiding-star,
my mum, for teaching me how to be loyal to myself and helping me become who I am
today. Life has given me a lot of lessons, but it was you who helped me to interpret
them and gave me the strength and values to stand up for myself. To my sister, we have
been through a lot together… But we are two strong branches of the same tree, and we
stay united and true. My admiration for you has no end, and no matter what life brings
you, you will always have me to lean on. To my brother-in-law, for being so unique and
special. To my second mum, Neia, for giving me the greatest gift of all: unconditional
love. To my “little sister” Dani, for showing me the poem that will accompany me for
the rest of my life and for guiding me so many times. To my family in Lisbon, thank
you for giving me so many reasons to smile, especially my small princesses that bring
me so much joy.
E porque a amizade é o maior ingrediente na receita da vida, finalmente um
agradecimento a todos os meus amigos. Ao pessoal de Viseu, em especial à Suzy,
Joaninha, Rafa, Mafaldinha e Vitinho, obrigada pela força. Um agradecimento especial
à Marta e à Xanó, que ao longo dos últimos 13 anos têm sido as minhas “soul sisters”.
Ao pessoal de Lisboa, entre muitos: o Afilhado, o Zica, a Marta e Fáfá (a dupla que
tanto me aguentou…), o Duarte e Inês, foi um prazer partilhar estes anos com vocês e
receber tanto.
E por último, a um amigo especial, que me convenceu que a vida dá mesmo
muitas voltas. Ao Filipe, que trouxe de volta o brilho nos meus olhos e o meu lado de
criança: Obrigado por seres exatamente como és, porque o que muitos não vêm é
exatamente o teu tesouro. Que saibamos sempre apreciar a união da tempestade com a
calmaria.
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