gene April 1991 W. Szybalski — Editor-in-Chief CONTENTS I, Oi: sieriscensisesisnsincisnacdsnsiniianiintantesinbinenitentimisitentin | Froeliger, E.H. and Leong, S.A, .............:cs:scsseseseseees 113-122 Editorial - The one-hundredth volume of Gene; a new The a mating-type alleles of Ustilago maydis are idio- milestone morphs Messing, J. (with Editor’s Note) Hicks, K.E., Read, M., Holloway, S.P., Sims, P.F.G. and Cloning in M13 phage or how to use biology at its best I Ei siinssccitnsehssnruicsonbanictclinsidntihecanenectiindenttt 123-129 Szybalski, W., Kim, S.C., Hasan, N. and Glycolytic pathway of the human malaria parasite Plas- SII UIE concsianteanitindanthatenienounenttaiaamieeaiahinimasibes 13-26 modium falciparum: primary sequence analysis of the gene Class-IIS restriction enzymes — a review encoding 3-phosphoglycerate kinase and chromosomal Blanco, L., Bernad, A., Blasco, M.A. and Salas, M. 27-38 mapping studies A general structure for DNA-dependent DNA polymer- Wang, X., Ooi, B.G. and Miller, L.K. ..................... 131-137 ases Baculovirus vectors for multiple gene expression and for Anand, N.N., Dubuc, G., Phipps, J., MacKenzie, C.R., occluded virus production Sadowska, J., Young, N.M., Bundle, D.R. and Yamazoe, M., Nakai, S., Ogasawara, N. and TMs. issincicasisteicsinitiatitanseeneenandvionineaniancaiabinaiien 39-44 II, cswtesicenindsieniesssncinsennicannieniteesbenbaanenisai 139-146 Synthesis and expression in Escherichia coli of cistronic Integration of woodchuck hepatitis virus (WHV) DNA at DNA encoding an antibody fragment specific for a Sal- two chromosomal sites (V,, and gag-like) in a hepatocellu- monella serotype B O-antigen lar carcinoma Kim, S.C., Pésfai, G. and Szybalski, W. ................. 45-50 Zhao, L.-J., Irie, K., Trirawatanapong, T., Nakano, R., A novel gene-fusing vector: construction of aS -GGmCC- Nakashima, A., Morimatsu and specific chimeric methyltransferase, M : BspRI/M : BsuRI Padmanabhan, R. ..................:.ss.csssscssssssscessesseeees 147-154 Ps Bx BIN I, Whe. sincoreceerscsstsnnseniianctnniaasasiines 51-57 Synthesis of biologically active adenovirus preterminal Mapping catalytically important regions of an enzyme protein in insect cells using a baculovirus vector using two-codon insertion mutagenesis: a case study cor- Sitzler, S., Oldenburg, I., Petersen, G. and relating f-lactamase mutants with the three-dimensional Bautz, E.K.F. ............. 155-162 structure Analysis of the promoter region of the housekeeping gene NG Re sidisiscescaicssisstidsecinaiasssnnssieltinaemaiiocmntniencsnniions 59-64 DmRP 140 by sequence comparison of Drosophila melano- An over-expression plasmid for Escherichia coli primase gaster and Drosophila virilis Haugland, R.A., Sangodkar, U.M.X., Sferra, P.R. and Craig, A.M. and Denhardt, D.T. 163-171 SN LIER. ciciccctcectanscincninictnatedimuntcuniodamnin 65-73 The murine gene encoding secreted phosphoprotein | (os- Cloning and characterization of a chromosomal DNA teopontin): promoter structure, activity, and induction in region required for growth on 2,4,5-T by Pseudomonas vivo by estrogen and progesterone cepacia AC1100 Chung, S. and Perry, R.P. ...... 173-180 Peo, TRS, cme Cinta, TEA. .cccccecascscsescccrsiscscsnvensccones 75-83 Cell-free transcription of a mouse ribosomal-protein- Bidirectional promoter and terminator regions bracket encoding gene: the effects of promoter mutations mmr, a resistance gene embedded in the Streptomyces Mouchiroud, D., D’Onofrio, G., Aissani, B., Macaya, G., coelicolor A3(2) gene cluster encoding methylenomycin Gautier, C. and Bernardi, G. 181-187 production The distribution of genes in the human genome Steyn, A.J.C. and Pretorius, 1S. 20.0.0... 85-93 Co-expression of a Saccharomyces diastaticus glucoamy- Short Communications lase-encoding gene and a Bacillus amyloliquefaciens «- Vieira, J. and Messing, J. .... 189-194 amylase-encoding gene in Saccharomyces cerevisiae New pUC-derived cloning vectors with different select- Lambrechts, M.G., Pretorius, 1.S., Sollitti, P. and able markers and DNA replication origins Wang, R.F. and Kushner, S.R. 195-199 Construction of versatile low-copy-number vectors for encoding gene (STA2) in Saccharomyces diastaticus cloning, sequencing and gene expression in Escherichia coli Unkles, S.E., Moon, R.P., Hawkins, A.R., Duncan, J.M. Kaniga, K. and Davison, J. 201-205 GID TIT, Dale seicccntccncstcnnsinsinsinnsninenscvinnannnnen 105-112 Tranposon vectors for stable chromosomal integration of Actin in the oomycetous fungus Phytophthora infestans is cloned genes in rhizosphere bacteria the product of several genes (Continued on page 349) gene April 1991 W. Szybalski — Editor-in-Chief CONTENTS I, Oi: sieriscensisesisnsincisnacdsnsiniianiintantesinbinenitentimisitentin | Froeliger, E.H. and Leong, S.A, .............:cs:scsseseseseees 113-122 Editorial - The one-hundredth volume of Gene; a new The a mating-type alleles of Ustilago maydis are idio- milestone morphs Messing, J. (with Editor’s Note) Hicks, K.E., Read, M., Holloway, S.P., Sims, P.F.G. and Cloning in M13 phage or how to use biology at its best I Ei siinssccitnsehssnruicsonbanictclinsidntihecanenectiindenttt 123-129 Szybalski, W., Kim, S.C., Hasan, N. and Glycolytic pathway of the human malaria parasite Plas- SII UIE concsianteanitindanthatenienounenttaiaamieeaiahinimasibes 13-26 modium falciparum: primary sequence analysis of the gene Class-IIS restriction enzymes — a review encoding 3-phosphoglycerate kinase and chromosomal Blanco, L., Bernad, A., Blasco, M.A. and Salas, M. 27-38 mapping studies A general structure for DNA-dependent DNA polymer- Wang, X., Ooi, B.G. and Miller, L.K. ..................... 131-137 ases Baculovirus vectors for multiple gene expression and for Anand, N.N., Dubuc, G., Phipps, J., MacKenzie, C.R., occluded virus production Sadowska, J., Young, N.M., Bundle, D.R. and Yamazoe, M., Nakai, S., Ogasawara, N. and TMs. issincicasisteicsinitiatitanseeneenandvionineaniancaiabinaiien 39-44 II, cswtesicenindsieniesssncinsennicannieniteesbenbaanenisai 139-146 Synthesis and expression in Escherichia coli of cistronic Integration of woodchuck hepatitis virus (WHV) DNA at DNA encoding an antibody fragment specific for a Sal- two chromosomal sites (V,, and gag-like) in a hepatocellu- monella serotype B O-antigen lar carcinoma Kim, S.C., Pésfai, G. and Szybalski, W. ................. 45-50 Zhao, L.-J., Irie, K., Trirawatanapong, T., Nakano, R., A novel gene-fusing vector: construction of aS -GGmCC- Nakashima, A., Morimatsu and specific chimeric methyltransferase, M : BspRI/M : BsuRI Padmanabhan, R. ..................:.ss.csssscssssssscessesseeees 147-154 Ps Bx BIN I, Whe. sincoreceerscsstsnnseniianctnniaasasiines 51-57 Synthesis of biologically active adenovirus preterminal Mapping catalytically important regions of an enzyme protein in insect cells using a baculovirus vector using two-codon insertion mutagenesis: a case study cor- Sitzler, S., Oldenburg, I., Petersen, G. and relating f-lactamase mutants with the three-dimensional Bautz, E.K.F. ............. 155-162 structure Analysis of the promoter region of the housekeeping gene NG Re sidisiscescaicssisstidsecinaiasssnnssieltinaemaiiocmntniencsnniions 59-64 DmRP 140 by sequence comparison of Drosophila melano- An over-expression plasmid for Escherichia coli primase gaster and Drosophila virilis Haugland, R.A., Sangodkar, U.M.X., Sferra, P.R. and Craig, A.M. and Denhardt, D.T. 163-171 SN LIER. ciciccctcectanscincninictnatedimuntcuniodamnin 65-73 The murine gene encoding secreted phosphoprotein | (os- Cloning and characterization of a chromosomal DNA teopontin): promoter structure, activity, and induction in region required for growth on 2,4,5-T by Pseudomonas vivo by estrogen and progesterone cepacia AC1100 Chung, S. and Perry, R.P. ...... 173-180 Peo, TRS, cme Cinta, TEA. .cccccecascscsescccrsiscscsnvensccones 75-83 Cell-free transcription of a mouse ribosomal-protein- Bidirectional promoter and terminator regions bracket encoding gene: the effects of promoter mutations mmr, a resistance gene embedded in the Streptomyces Mouchiroud, D., D’Onofrio, G., Aissani, B., Macaya, G., coelicolor A3(2) gene cluster encoding methylenomycin Gautier, C. and Bernardi, G. 181-187 production The distribution of genes in the human genome Steyn, A.J.C. and Pretorius, 1S. 20.0.0... 85-93 Co-expression of a Saccharomyces diastaticus glucoamy- Short Communications lase-encoding gene and a Bacillus amyloliquefaciens «- Vieira, J. and Messing, J. .... 189-194 amylase-encoding gene in Saccharomyces cerevisiae New pUC-derived cloning vectors with different select- Lambrechts, M.G., Pretorius, 1.S., Sollitti, P. and able markers and DNA replication origins Wang, R.F. and Kushner, S.R. 195-199 Construction of versatile low-copy-number vectors for encoding gene (STA2) in Saccharomyces diastaticus cloning, sequencing and gene expression in Escherichia coli Unkles, S.E., Moon, R.P., Hawkins, A.R., Duncan, J.M. Kaniga, K. and Davison, J. 201-205 GID TIT, Dale seicccntccncstcnnsinsinsinnsninenscvinnannnnen 105-112 Tranposon vectors for stable chromosomal integration of Actin in the oomycetous fungus Phytophthora infestans is cloned genes in rhizosphere bacteria the product of several genes (Continued on page 349) (Contents continued from back cover) Perego, M., Wu, J.-J., Spiegelman, G.B. and Bilang, R., lida, S., Peterhans, A., Potrykus, I. and Hoch, J.A. 207-212 Paszkowski, J. 247-250 Mutational dissociation of the positive and negative properties The 3’-terminal region of the hygromycin-B-resistance gene is of the Spo0A sporulation transcription factor of Bacillus subtilis important for its activity in Escherichia coli and Nicotiana tabacum Lange, C., Noyer-Weidner, M., Trautner, T.A., Weiner, M. and Slightom, J.L. 251-255 Zahler, S.A. 213-218 Custom polymerase-chain-reaction engineering of a plant M-H2I, a multispecific SC-DNA methyltransferase encoded by expression vector Bacillus amyloliquefaciens phage H2 Tanaka, H., Yamada, M., Kishi, F. and Nakazawa, A. 257-260 Andrews, J., Minter, S.J. and Davies, R.W. ...............0000+ 219-224 Characterization of a processed pseudogene derived from the Production of mutant dihydrofolate reductases of Lactobacillus gene encoding bovine adenylate kinase isozyme 2 casei for nuclear magnetic resonance spectroscopy Bosma, P.J., Kooistra, T., Siemieniak, D.R. and Lane, M.A., Bayles, K.W. and Yasbin, R.E. 225-229 Slightom, J.L. 261-266 Identification and initial characterization of glucose-repressible Further characterization of the 5’-flanking DNA of the gene promoters of Streptococcus mutans encoding human plasminogen activator inhibitor-1 Donadio, S. and Hutchinson, C.R. 231-235 Cloning and characterization of the Saccharopolyspora erythraea Brief Note fdxA gene encoding ferredoxin Brinkley, P., Bautista, D.S. and Graham, F.L. Hawker, K.L., Montague, P., Marzluf, G.A. and The cleavage site of restriction endonuclease MniI Kinghorn, J.R. 237-240 Heterologous expression and regulation of the Neurospora crassa nit-4 pathway-specific regulatory gene for nitrate assimilation in Founding Editors and Editors - Past and Present Aspergillus nidulans Photographs and Informal Biographies 271-290 Burland, T.G., Pallotta, D., Tardif, M.C., Lemieux, G. and Dove, W.F. . 241-245 Fission yeast promoter-probe vectors based on hygromycin re- Name Index Volumes 1-100 291-348 sistance Contents continued from back cover