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Food Science and Food Biotechnology EDITED BY Gustavo F. Gutiérrez-López, Ph.D. Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional México, DF. Gustavo V. Barbosa-Cánovas, Ph.D. Washington State University Pullman, Washington CRC PR ESS Boca Raton London New York Washington, D.C. TX68926_FM.fm Page 4 Wednesday, January 29, 2003 9:38 AM Library of Congress Cataloging-in-Publication Data Food science and food biotechnology / edited by Gustavo F. Gutiérrez-López and Gustavo V. Barbosa-Cánovas. p. cm. -- (Food preservation technology series) Includes bibliographical references and index. ISBN 1-56676-892-6 1. Food--Biotechnology. 2. Food industry and trade. I. Gutiérrez-López, Gustavo F. II. Barbosa-Cánovas, Gustavo V. III. Series TP248.65.F66 F733 2003 664--dc21 2002035052 This book contains information obtained from authentic and highly regarded sources. Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed. Reasonable efforts have been made to publish reliable data and information, but the authors and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use. Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, microfilming, and recording, or by any information storage or retrieval system, without prior permission in writing from the publisher. All rights reserved. Authorization to photocopy items for internal or personal use, or the personal or internal use of specific clients, may be granted by CRC Press LLC, provided that $1.50 per page photocopied is paid directly to Copyright Clearance Center, 222 Rosewood Drive, Danvers, MA 01923 USA. The fee code for users of the Transactional Reporting Service is ISBN 1-56676-892-6/03/ $0.00+$1.50. The fee is subject to change without notice. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. The consent of CRC Press LLC does not extend to copying for general distribution, for promotion, for creating new works, or for resale. Specific permission must be obtained in writing from CRC Press LLC for such copying. Direct all inquiries to CRC Press LLC, 2000 N.W. Corporate Blvd., Boca Raton, Florida 33431. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation, without intent to infringe. Visit the CRC Press Web site at www.crcpress.com © 2003 by CRC Press LLC No claim to original U.S. Government works International Standard Book Number 1-56676-892-6 Library of Congress Card Number 2002035052 Printed in the United States of America 1 2 3 4 5 6 7 8 9 0 Printed on acid-free paper TX68926_FM.fm Page 5 Wednesday, January 29, 2003 9:38 AM Dedication To our families © 2003 CRC Press LLC TX68926_FM.fm Page 7 Wednesday, January 29, 2003 9:38 AM Contents 1 Introduction to Molecular Food Biotechnology Juan Alberto Osuna-Castro and Octavio Paredes-López 2 Recent Developments in Food Biotechnology Humberto Hernández-Sánchez 3 Bioprocess Design Ali Asaff-Torres and Mayra De la Torre-Martínez 4 Gas Hold-Up Structure in Impeller Agitated Aerobic Bioreactors Ashok Khare and Keshavan Niranjan 5 Production and Partial Purification of Glycosidases Obtained by Solid-State Fermentation of Grape Pomace Using Aspergillus niger 10 Sergio Huerta-Ochoa, María Soledad de Nicolás-Santiago, Wendy Dayanara Acosta-Hernández, Lilia Arely Prado-Barragán, Gustavo Fidel Gutiérrez-López, Blanca E. García-Almendárez, and Carlos Regalado-González 6 Protein Crystallography Impact on Biotechnology Manuel Soriano-García 7 Stability of Dry Enzymes Mauricio R. Terebiznik, Viviana Taragano, Vanessa Zylberman, and Ana María Pilosof 8 Trends in Carotenoids Biotechnology María Eugenia Jaramillo-Flores and Rosalva Mora-Escobedo 9 Studies on the Reverse Micellar Extraction of Peroxidase from Cruciferae Vegetables of the Bajio Region of México Carlos Regalado-González, Miguel A. Duarte-Vázquez, Sergio Huerta- Ochoa, and Blanca E. García-Almendárez © 2003 CRC Press LLC TX68926_FM.fm Page 8 Wednesday, January 29, 2003 9:38 AM 10 Improving Biogeneration of Aroma Compounds by In Situ Product Removal Leobardo Serrano-Carreón 11 Lupines: An Alternative for Debittering and Utilization in Foods Cristian Jiménez-Martínez, Humberto Hernández-Sánchez, and Gloria Dávila-Ortíz 12 Recent Development in the Application of Emulsifiers: An Overview Victor T. Huang 13 Drying of Biotechnological Products: Current Status and New Developments Arun S. Mujumdar 14 An Update on Some Key Alternative Food Processing Technologies: Microwave, Pulsed Electric Field, High Hydrostatic Pressure, Irradiation, and Ultrasound José J. Rodríguez, Gustavo V. Barbosa-Cánovas, Gustavo Fidel Gutiérrez-López, Lidia Dorantes-Alvárez, Hye Won Yeom, and Q. Howard Zhang 15 Emerging Processing and Preservation Technologies for Milk and Dairy Products Valente B. Alvarez and Taehyun Ji © 2003 CRC Press LLC TX68926_FM.fm Page 9 Wednesday, January 29, 2003 9:38 AM Series Preface I am glad to welcome this book on food science and food biotechnology to the growing CRC Food Preservation Technology Series. This new book sig- nificantly expands the scope of the series, opening new directions I would like to pursue. The blend of engineering with food science and food biotech- nology is quite unique, as well as complex and intriguing. Wonderful things will come from this association, provided we make the right considerations and identify those products that match expectations. Biotechnology is vast, without boundaries, and offers great possibilities as well as concerns. Let’s use it correctly, let’s learn more about it, and let’s see how it will contribute to the science of making better foods on a daily basis. I am very enthusiastic about this book and hope that all readers will feel the same. It has significant new material that will open new horizons; and it offers the opportunity to expand two critical disciplines at the same time: food biotechnology and food science. Gustavo V. Barbosa-Cánovas © 2003 CRC Press LLC TX68926_FM.fm Page 11 Wednesday, January 29, 2003 9:38 AM Preface The relevance of biotechnology in food science has significantly grown in the last few years and this trend will continue in the years to come. For this reason, it is becoming quite relevant to identify in an organized manner efforts toward the use of a very important area of scientific research and application (biotechnology) in a domain of tremendous relevance for the whole population (food science). This book presents a meaningful and up-to-date review of how food sci- ence and biotechnology are interacting and covers important aspects of both subjects as well as their interface. Distinguished scientists from key institu- tions have contributed chapters that provide deep analysis of their particular subjects and at the same time place each topic within the context of this interface. The premise of this book is that an effective discussion on these subjects (food science, biotechnology) requires an effective coupling to con- vey in a comprehensive manner the state-of-the-art for the fundamentals and applications of both areas. The book is mainly directed to academics, undergraduate and postgrad- uate students (including research students) in food science and technology, biotechnology, and bioengineering, who will find a selection of topics rang- ing from the molecular basis of food preservation and biotechnology to industrial applications. Professionals working in food and biotechnology research centers also may find this book useful. The first chapter reviews molecular aspects of food biotechnology, present- ing the reader a state-of-the-art introduction to the next 14 chapters. The second chapter covers some of the recent developments in food biotechnol- ogy. The reader may note a certain degree of overlap between these two chapters. However, Chapter 2 covers a wide range of food applications. Chapters 3, 4, and 5 describe specific examples of upstream processes and bioseparations. The following eight chapters cover basic and applied situations of food biotechnology such as the impact of crystallography, stability of enzymes, biotechnology of carotenoids, biogeneration of aroma compounds, potential usage of regional alternative food supplies, and developments in the appli- cation of emulsifiers. The last three chapters describe food preservation techniques, strongly stressing alternative food processing technologies as well as drying of biotechnological products. It is very likely that many of the procedures and techniques described in the book will be used in the food biotechnology industry. It is hoped that this text will constitute a worthy addition to the emerging literature on food biotechnology and that the readers will find in it balanced and organized © 2003 CRC Press LLC TX68926_FM.fm Page 12 Wednesday, January 29, 2003 9:38 AM information, with chapters written with the common goal of stressing the interactions of food science and biotechnology and demonstrating, through specific examples, that the bases for many of the situations discussed in the various sections are very similar. Gustavo F. Gutiérrez-López Gustavo V. Barbosa-Cánovas © 2003 CRC Press LLC TX68926_FM.fm Page 13 Wednesday, January 29, 2003 9:38 AM Editors Gustavo F. Gutiérrez-López received his bachelor of science in biochemical engineering and master of science in food science and technology from the National Polytechnic Institute of Mexico, and his master of science in food process engineering and Ph.D. in food engineering from the University of Reading, U.K. He is currently professor of food engineering at the National School of Biological Sciences of the National Polytechnic Institute of Mexico and president of the Mexican Society of Biotechnology and Bioengineering. Gustavo V. Barbosa-Cánovas received his bachelor of science in mechanical engineering from the University of Uruguay and his master of science and Ph.D. in food engineering from the University of Massachusetts at Amherst. He is currently professor of food engineering at Washington State University and director of the Center for Nonthermal Processing of Food. © 2003 CRC Press LLC TX68926_FM.fm Page 15 Wednesday, January 29, 2003 9:38 AM Acknowledgments The Editors wish to express their gratitude to the following institutions and individuals who contributed to making this book possible: National School of Biological Sciences of the National Polytechnic Institute of Mexico (ENCB-IPN), CYTED (Ibero-American Program to promote sci- ence and technology) Subprogram XI, Mexican Society of Biotechnology and Bioengineering (SMBB), and Washington State University (WSU) for sup- porting the preparation of this book. Our fellow colleagues Lidia Dorantes, María Eugenia Jaramillo, Rosalva Mora, Gloria Dávila, and Humberto Hernández (IPN-ENCB) for their valu- able comments and suggestions throughout the preparation of this book. J. Anderson (WSU) for her professionalism and dedication throughout the entire editorial process. V. Aguilar-Clark, L. Alamilla, M. Cornejo, A. Ortíz, and J. Chanona, all from ENCB-IPN, and Gipsy Tabilo (WSU) for their decisive participation in helping the editors prepare the final version of this book by revising refer- ences, formatting all the manuscripts, and incorporating in the text all the editorial comments. © 2003 CRC Press LLC

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