Food Immunoassay

371 Pages·2019·16.698 MB·English

by  Chuanlai Xu, Hua Kuang, Liguang Xu

Chuanlai Xu · Hua Kuang · Liguang Xu
Food
Immunoassay
Food Immunoassay
Chuanlai Xu Hua Kuang Liguang Xu
(cid:129) (cid:129)
Food Immunoassay
123
ChuanlaiXu Hua Kuang
Schoolof FoodScience andTechnology Schoolof FoodScience andTechnology
JiangnanUniversity JiangnanUniversity
Wuxi,China Wuxi,China
Liguang Xu
Schoolof FoodScience andTechnology
JiangnanUniversity
Wuxi,China
ISBN978-981-13-9033-3 ISBN978-981-13-9034-0 (eBook)
https://doi.org/10.1007/978-981-13-9034-0
Projectno.:2017-2-148
©SpringerNatureSingaporePteLtd.2019
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Acknowledgements
Thanks for the list of contributors below who have contributed to this book.
Chuanlai Xu
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Hua Kuang
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Liguang Xu
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Yanni Chen
CollegeofFoodScienceandEngineering,CentralSouthUniversityofForestryand
Technology.
E-mail: [email protected]
Dezhao Kong
School of Grain Science and Technology, Jiangsu University of Science and
Technology.
E-mail: [email protected]
Changrui Xing
Nanjing University of Finance and Economics.
E-mail: [email protected]
Xiujin Chen
CollegeofFoodandBioengineering,HenanUniversityofScienceandTechnology.
E-mail: [email protected]
v
vi Acknowledgements
Zhongxing Wang
State Key Lab of Food Science and Technology, Jiangnan University.
E-mail: [email protected].
Naifeng Xu
Institute of Food Engineering, College of Life Science, Shanghai Normal
University.
E-mail: [email protected]
Zhuokun Li
College of Food Science, Fujian Agriculture and Forestry University.
E-mail: [email protected]
Lingling Guo
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Wenbin Wang
Collegeofmarinelifeandfisheries,HuaihaiInstituteofTechnology,Lianyungang,
Jiangsu, P.R. China.
E-mail: [email protected]
Yue Li
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Liqiang Liu
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Wei Ma
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Xiaoling Wu
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Shanshan Song
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Jianping Zhu
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Haiying Liu
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Acknowledgements vii
Maozhong Sun
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Changlong Hao
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Si Li
School of Food Science and Technology, Jiangnan University.
E-mail: [email protected]
Contents
1 Introduction of Immunoassays . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 ELISA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2.1 Principle of ELISA. . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.2.2 Classification of ELISA . . . . . . . . . . . . . . . . . . . . . . . 2
1.2.3 Preparation of mAb . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.2.4 Establishment of Indirect and Competitive ELISA . . . . 7
1.3 Development of Strip Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
1.3.1 Principle of Strip Test. . . . . . . . . . . . . . . . . . . . . . . . . 11
1.3.2 Steps for Strip Test . . . . . . . . . . . . . . . . . . . . . . . . . . 12
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2 Mycotoxin Immunoassay in Food . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.1.1 Aflatoxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.1.2 Ochratoxin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.1.3 Fumonisins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
2.1.4 Trichothecenes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
2.1.5 Zearalenone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
2.1.6 Sterigmatocystin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
2.1.7 Citrinin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
2.1.8 Alternaria Toxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
2.1.9 Patulin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
2.1.10 Other Mycotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
2.2 Design and Synthesis of Antigen. . . . . . . . . . . . . . . . . . . . . . . 31
2.2.1 Aflatoxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
2.2.2 Ochratoxin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
2.2.3 Fumonisins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
2.2.4 Trichothecenes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
2.2.5 Zearalenone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
ix
x Contents
2.2.6 Sterigmatocystin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
2.2.7 Citrinin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
2.2.8 Alternaria Toxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2.2.9 Patulin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
2.2.10 Other Mycotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
2.3 ELISA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
2.3.1 Aflatoxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
2.3.2 Ochratoxin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
2.3.3 Fumonisins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
2.3.4 Trichothecenes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
2.3.5 Zearalenone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
2.3.6 Sterigmatocystin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
2.3.7 Citrinin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
2.3.8 Alternaria Toxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
2.3.9 Patulin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
2.4 Colloidal Gold Immunochromatographic Strips. . . . . . . . . . . . . 48
2.5 Perspectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
3 Heavy Metal Immunoassay in Food . . . . . . . . . . . . . . . . . . . . . . . . 53
3.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
3.2 Design and Synthesis of Antigen. . . . . . . . . . . . . . . . . . . . . . . 58
3.2.1 Lead . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
3.2.2 Cadmium. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
3.2.3 Mercury. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
3.2.4 Chromium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
3.2.5 Copper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
3.3 Enzyme-Linked Immunosorbent Assay. . . . . . . . . . . . . . . . . . . 62
3.4 Colloidal Gold Lateral Flow Assay . . . . . . . . . . . . . . . . . . . . . 64
3.5 Perspectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
4 Pesticide Immunoassay in Food . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
4.1 Pesticide Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
4.1.1 Pyrethroid Insecticides . . . . . . . . . . . . . . . . . . . . . . . . 69
4.1.2 Organic Phosphorus Insecticide. . . . . . . . . . . . . . . . . . 72
4.1.3 Neonicotinoid Insecticides . . . . . . . . . . . . . . . . . . . . . 74
4.1.4 Triazole Fungicides . . . . . . . . . . . . . . . . . . . . . . . . . . 74
4.1.5 Strobilurin Fungicides. . . . . . . . . . . . . . . . . . . . . . . . . 77
4.1.6 Anilinopyrimidine Fungicides . . . . . . . . . . . . . . . . . . . 80
4.1.7 Amide Fungicides . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
4.1.8 Herbicide Pesticides . . . . . . . . . . . . . . . . . . . . . . . . . . 83
4.1.9 Other Pesticides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Contents xi
4.2 Design and Synthesis of Antigen (Hapten) . . . . . . . . . . . . . . . . 94
4.2.1 Design and Synthesis of Pyrethroid Antigens. . . . . . . . 95
4.2.2 Design and Synthesis of Organophosphorus
Insecticides Antigen . . . . . . . . . . . . . . . . . . . . . . . . . . 97
4.2.3 Design and Synthesis of Neonicotinoid Insecticides
Antigen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
4.2.4 Design and Synthesis of Triazole Fungicides
Antigen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
4.2.5 Design and Synthesis of Strobilurin Fungicides
Antigen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
4.2.6 Design and Synthesis of Anilinopyrimidine
Fungicides Haptens . . . . . . . . . . . . . . . . . . . . . . . . . . 108
4.2.7 Design and Synthesis of Trifloxystrobin
Antigen Amide Fungicides . . . . . . . . . . . . . . . . . . . . . 115
4.2.8 Design and Synthesis of Bensulfuron-Methyl
Herbicide Antigen . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
4.2.9 Other Pesticides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
4.3 Enzyme-Linked Immunosorbent Assay. . . . . . . . . . . . . . . . . . . 127
4.3.1 ELISA for Pyrethroids Analysis . . . . . . . . . . . . . . . . . 127
4.3.2 ELISA of Organophosphorus Pesticides. . . . . . . . . . . . 129
4.3.3 ELISA of Neonicotinoid Insecticides. . . . . . . . . . . . . . 131
4.3.4 ELISA of Triazole Fungicides. . . . . . . . . . . . . . . . . . . 133
4.3.5 ELISA of Strobilurin Fungicides. . . . . . . . . . . . . . . . . 134
4.3.6 ELISA of Anilinopyrimidine Fungicides . . . . . . . . . . . 138
4.3.7 ELISA of Amide Fungicides. . . . . . . . . . . . . . . . . . . . 140
4.3.8 ELISA of Herbicide Pesticides . . . . . . . . . . . . . . . . . . 142
4.3.9 ELISA of Other Pesticides . . . . . . . . . . . . . . . . . . . . . 143
4.4 Strip Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
4.5 Perspectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
5 b-Agonist and Hormone Immunoassays in Food. . . . . . . . . . . . . . . 155
5.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
5.2 Design and Synthesis of Antigen. . . . . . . . . . . . . . . . . . . . . . . 158
5.2.1 b-Agonists. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
5.2.2 Artificial Hormone . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
5.3 Enzyme-Linked Immunosorbent Assay. . . . . . . . . . . . . . . . . . . 169
5.3.1 Sample Pretreatment. . . . . . . . . . . . . . . . . . . . . . . . . . 169
5.3.2 Enzyme-Linked Immunosorbent Assay (ELISA) . . . . . 170
5.4 Colloidal Gold Immunochromatographic Strips. . . . . . . . . . . . . 171
5.5 Perspectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173