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Basic Research Journal of Agricultural Science and Review ISSN 2315-6880 Vol. 3(12) pp. 146-160 December 2014 Available online http//www.basicresearchjournals.org Copyright ©2014 Basic Research Journal Full Length Research Paper Fertilizer N- and P-rates Response on sunflower intercropping with Mungbean in North-West, Pakistan Murad Ali Khan1, Mohammad Akmal1 and M. Afzal2 1-2Department Agronomy, University of Agriculture, Peshawar, Pakistan. 3Department of SES, The University of Agriculture, Peshawar, Pakistan. *Corresponding author email. [email protected]: Tel. +92-91-9218597 Accepted 18 November, 2014 Abstract Intercrop mungbean in sunflower may increase yield, improve soil quality and use the resources (e.g. air, water and light) more efficiently. We, therefore, compared mungbean intercropped in sunflower by increasing N and P fertilizer rates. The given fertilizers rates were 30, 60, 90 kg ha-1 each for N and P O 2 5 including a control treatment (no N and P fertilizer) as sole sunflower, mungbean and their intercropping in spring 2011 and 2012. The experiment was conducted in randomized complete block design with split plot arrangement at Agronomy Research Farm, the University of Agriculture Peshawar, Pakistan. Results revealed that grain yield (kg ha-1) of sunflower crop did not differ as sole or intercrop but did reported higher for mungbean as sole than intercrop which were due to healthier traits of the mungbean crop as sole than intercrop. Fertilized than control obviously showed better yield and yield traits of both sunflower and mungbean. Increased N-rates from 30 to 90 kg ha-1 showed a significant (p<0.05) increase in yield of both sunflower and mungbean. This increase in yield of crops were mainly associated with increase in head diameter, grain number per head and grains weight of sunflower and likewise the pod number, grain per pod and grains weight of mungbean. Grain oil content of sunflower and nodule number of mungbean were rather adversely affected by increase in N-rate to the crops. Increase in P-rate to both crops increased grain yield with markedly increase in thousand grains weight on sunflower and mungbean. The study suggested that intercrop mungbean in sunflower than sole crop has a potential for further yield improvement of the cropping system. Research needs to identify an appropriate variety as well as species for the better soil health and future production per unit return. Keywords: N and P-rates, cropping systems, grain yield of sunflower and mungbean, yield traits of sole and intercrop INTRODUCTION World population is consistently increasing at a very animal. Food production in Asia is growing well with a faster rate. Nonetheless, this increase in world population variety of crops and variations in productions of the is reported higher in developing countries as compared to crops. Growers are adopting different methodology for the develop countries. Asia in comparison to the rest of increasing production of the food crops at par with the continents is densely populated with human and population increase. However, huge variation still exist in Published by Basic Research Journal of Agricultural Science and Review Khan et al. 147 the amount of food production, their availability at par related issues has also adversely affected its cultivation with the existing population of the different countries. For (PODP Annual Report, 2013). With a fragile canopy example, Pakistan is acute deficient in the production of volume, sunflower can be intercrop with other crops (e.g. edible oil (Munir et al., 2008). By consistently growing mungbean, mash-bean, cowpea, groundnut etc.) population, the condition is getting more horrible every because Pakistan is already acute deficient in food- next day (Iqbal et al., 2007). The density per unit area of legumes and fodder (Fraz et al., 2006). Mungbean seeds population and urbanization is continuously expanding, contain 20-25% protein and famous as a poor man’s which has unfolded the demand and indigenous oilseed meat (Potter and Hotchkiss, 1997). Biological N-fixation is production, resulting a continuous price increase and a additional importance for the legumes. Pakistan’s soil is sever decrease in the edible oil quality (Awais et al., also poor in fertility and cost of fertilizer is highly 2013). A rapid increase of local oil production should be fluctuating (Elahi et al., 2004). Imbalanced nutrient the major concern for decision makers and experts to application with low soil fertility is major constraint limiting control import bill and make available the affordable productivity of many crops in the developing countries edible oil to low-income population that is in vast majority. (Awais et al., 2013). Intercropping is, therefore, a vital Among the different growing oil seed crops, sunflower agricultural production system where two crops could be (Helianthus annuus L.) is a non-conventional edible developed simultaneously on a single piece of land for a oilseed crop, which has the ability to fulfill the existing period or full crop growth season for maximum resources gap between domestic demands and production (Hu et utilization (Iqbal et al., 2008). It is an improvement on al., 2008). It is widely distributed oil seed crop of the indigenous farming system by efficiently utilizing season world (Allam et al., 2003) due to its adaptation with a and markedly increasing yield (Caviglia et al., 2004). range of climatic conditions (Koutroubas et al., 2008). Sometime, crop is unable to compete economically with The maximum seed oil content in sunflower ranging from other crops in the cropping system, intercropping can 40 to 47% (Saleem et al., 2003; Nasim et al., 2011). extend opportunities to replace that crop for sustainable Among so many other factors, the desired quantity of farming system. An efficient and economical nutrients i.e. Nitrogen and Phosphorus are effective intercropping system depends largely on selection of a approach to improve sunflower yield (Ali et al., 2004; compatible crop or crop species that can be improved Ozer et al., 2004; Osman, 2010). The rate of N-fertilizer through appropriate nutrient management (N and P) and application to the crop is highly essential (Ali et al., 2012) well defined agronomic practices for relatively higher to increase local edible oil production (Nasim et al., returns from the companion crop (Imran et al., 2011). In 2011), which makes it competitive with other crops in the this study, the focus was to improve understanding of cropping system. Results have shown a favorable sunflower cultivation as intercrop with mungbean in response of N-fertilizers rates on photosynthesis, leaf relation to different nutrients e.g. nitrogen and area and leaf area index with increasing application of N- phosphorus rates. The yield and yield traits of sole main fertilizer (Rouphael et al., 2007). Research has also crop with intercropping were compared for future -1 cultivation. shown that N-fertilizer rate of 100 kg ha was optimum for the sunflower production and increased N-fertilizer -1 rates from 100 to 150 kg ha has a reverse effect on MATERIALS AND METHODS both yield and oil content (Oyinlola et al., 2010). Hussain et al. (2011) have reported that N-fertilizer application of Location and experiment -1 80 kg ha was sufficient to sunflower with the maximum yield. However, the N-fertilizer rate for the crop is mostly Field experiment was conducted at Agronomy Research associated with soil fertility, quantity of N-fertilizer applied Farm, the University of Agriculture Peshawar in spring to previous crop in the cropping system, environmental 2011 and 2012. Experimental site was located 34001″ N conditions of the area and crop N-uptake demand etc. and 71o35″ E at 359 m above the sea level. Environment (Laureti et al., 2007). Effective N-uptake and yield of the research farm was warm to hot, semi-arid performance has a close link with phosphorus application subtropical continental climate with mean annual and soil status (Awais et al., 2013). Efficiency of the given precipitation of about 360 mm. The soil was deep silt clay N could be improved with sufficient P-fertilizer application loam, alkaline, pH ranges about 7.5–7.8 but relatively rates to crops and its availability in soil on seed yield and deficient in N < 0.5 g kg-1. Rainfall and temperatures of quality i.e. the oil content (Iqbal et al., 2008). the crop growth seasons are shown in the Figure 1. Sunflower has a potential to grow as spring and Mungbean (Vigna radiate L.) as intercrop in the main summer season crops in Pakistan. However, its area sunflower (Helianthus annuus L.) were used to compare under cultivation has decreased since 2007 due to low yield and yield traits. Sunflower hybrid (Hysun-33) and economic returns as compared to maize in the cropping mungbean (Cv. Chakwal mung-06) were used to study system. Beside production, handling and marketing the response of 30, 60 and 90 kg ha-1 each of N and P O 2 5 Published by Basic Research Journal of Agricultural Science and Review 148. Basic Res. J. Agric.Sci. Rev. Figure 1. Weather data (temperature minimum and maximum, rainfall) of the crop growth season in spring 2011 and 2012 at Agronomy Research Farm, the University of Peshawar Pakistan. nutrients given to sole and intercrop with a control no N individual head by averaging all for that treatment. and P. Experiment was conducted in a randomized Similarly, ten representative plants of mungbean from complete block design (RCBD) in a split plots border rows were uprooted with soil at flowering stage. arrangement, where each treatment was replicated four The soil clumps were carefully washed and nodules were times. Experimental unit was 3.0 m in length and 4.2 m in detached from the mungbean plants. First the number width, accommodating six sunflower rows at 0.70 m were counted then the weight were recorded and distances. Sole mungbean was planted at 0.35 m, averaged for a single reading. accommodating 12 rows. For intercropping, alternate The number of pods plant-1 in mungbean was manually rows of mungbean were planted in between the counted by harvesting all pods on ten randomly selected sunflower rows. In addition to nutrients, one control plants taken from two central rows in a subplot and treatment was also included. Crops (sole and. intercrop) averaged for a final mean value. Grains head-1 as treatment were allotted to the main plots and fertilizer (sunflower) and pod-1 (mungbean) were counted rates (i.e. N and P) to the subplots. Urea and Single manually on ten randomly selected representative plants Super Phosphate (SSP) were used to yield N and P, in an experimental unit. Grains were manually shelled respectively. Both N and P were applied as band from sunflower heads and mungbean pods, already application in close proximity to the sunflower crop. All P counted at harvest for the desired data. All grains were was applied at sowing and N in two splits: half at sowing divided on total heads and pods for a mean reading. and remaining half 30 days after sowing (DAS). Plant Thousand grains weight (g) was recorded from threshed populations in experimental units were maintained by clean grains in a treatment by taking random samples manual thinning 20 DAS (20 cm) in sunflower and 35 and counting grains and independently weight on a DAS (10 cm) in mungbean. Crop was sown manually precise digital balance for each species. Two central within the second week of March in 2011 and 2012, rows in an experimental unit (sole and intercropped) were respectively. Sowing was made with a single row hand harvested; sun dried in field for two weeks, threshed driven drill using recommended seed rates for sunflower manually and weighed to record grains weight. A (6 kg ha-1) and mungbean (24 kg ha-1) as intercrop and subsample was oven dried at 60OC for 36 h and sole crop. Uniform agronomic practices and plant difference in weight was adjusted accordingly for grain protection measures were applied during the crop growth yield. to keep field free from weeds, insect pests and diseases. Oil content (%) in sunflower seed was carried out nondestructively using Near Infrared reflectance Spectroscopy (NIRS) at Nuclear Institute for Food and Measurement and observations Agriculture (NIFA) Peshawar. The whole seeds were placed in a cup spinner accessory combined with the Head diameter of sunflower crop was measured on ten integrating sphere modulus of the NIR equipment (Model randomly selected representative plants from the central Antaris II, Thermo Scientific). The NIRS technique rows of a subplot and final reading was noted from measures energy absorbed (1100-2500 nm) in whole Published by Basic Research Journal of Agricultural Science and Review Khan et al. 149 Table 1. ANOVA for different observation recorded in main (sunflower) and companion intercropped (Mungbean) during spring seasons 2011-12. Sunflower Mungbean SOV d.f. GY HD GPH TGW OC GNC TNC GY PPP GPP TGW NPP GNC TNC Year (Y) 1 NS NS NS NS NS NS NS NS NS NS NS NS NS NS Rep. within Y 6 NS NS NS NS NS NS NS NS NS NS NS NS NS NS Cropping System (CS) 1 NS NS NS NS NS NS NS ** ** ** ** ** ** * Y x CS 1 NS NS NS NS NS NS NS NS NS NS NS NS NS NS Error I 6 Treatment (T) 9 ** ** ** ** ** ** ** ** ** ** ** ** ** ** Control (C) vs. rest (R) (1) ** ** ** ** * ** * ** ** ** ** ** ** ** Phosphorus (P) (2) ** ** * ** * ** ** ** ** * ** ** ** ** Nitrogen (N) (2) ** ** ** ** ** ** ** ** ** ** ** ** ** ** N x P (4) ** ** NS NS * * * ** ** * * NS * ** CS x T 9 NS NS NS ** NS NS * ** ** ** NS ** NS NS CS x P (2) NS NS NS * NS NS NS ** ** ** NS * NS NS CS x N (2) NS NS NS NS NS NS NS ** ** ** NS ** NS NS CS x N x P (4) NS NS NS NS NS NS NS ** NS NS NS NS NS NS CS x C vs. R (1) NS NS NS NS NS * ** ** ** * NS ** NS NS Y x T 9 NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x P (2) NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x N (2) NS NS NS NS NS NS NS NS * NS NS NS NS NS Y x N x P (4) NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x C vs. R (1) NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x CS x T 9 NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x CS x P (2) NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x CS x N (2) NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x CS x N x P (4) NS NS NS NS NS NS NS NS NS NS NS NS NS NS Y x CS x C vs. R (1) NS NS NS NS NS NS NS NS NS NS NS NS NS NS Error II 144 Total 159 GY = grain yield, HD = head diameter, GPH =grains per head, TGW = 1000 grains weight, OC = Oil content, GNC = grain N content, PPP = pods per plant, GPP = grain per pod, NPP = nodules per plant, seed sample. The precision of NIRs methods was (PLS) regression, artificial neural network (ANN) H SO in presence of digestion mixture containing 2 4 based on previous reference methods already etc., obtained from NIR spectra and the primary K SO , CuSO and Se on a heating block for 2 4 4 calibrated stored in the system and oil contents data. This calibration model then can be used to about 4-5 h. The digestion was initiated at 50oC were expressed on 6 to 8 % moisture basis. First predict NIR spectrum of unknown oil seeds. and then raised temperature gradually to 100, a calibration of NIRS is needed to build a good The grains and tissue N-content was 150, 200, and 300 to 350oC. Final temperature calibration model (equation) for specific crop that determined by micro-Kjeldhal Method (Bremner was maintained for an hour by turning sample in can be used a chemometric tool such as multiple and Mulvaney, 1982). Briefly, about 0.2 g of to light greenish or color-less and then stop the linear regression (MLR), partial least squares sample was digested in 3 ml of concentrated further heating. At cooling, digest was transferred Published by Basic Research Journal of Agricultural Science and Review 150. Basic Res. J. Agric.Sci. Rev. Table 2. Yield and yield traits (averaged across N and P) of sunflower and mungbean as affected by the treatment sole and intercrop during spring 2011 and 2012. Crop & Status Yield Head Grains 1000 Grains Oil content Grain N-content Tissue N-content Sunflower (kg ha-1) Diameter (cm) head-1 weight (g) (%) (g kg-1) (g kg-1) 2011 Sole 2295 17.28 945 a 46.29 41.72 31.52 a 10.83 Intercrop 2266 16.77 934 b 45.52 41.44 30.70 b 10.48 2012 Sole 2318 17.59 953 46.50 42.67 31.79 11.07 a Intercrop 2291 17.14 947 45.91 41.64 31.10 10.54 b Mean Sole 2306 17.43 949 46.40 42.19 31.65 a 10.95 Intercrop 2278 16.96 941 45.72 41.54 30.90 b 10.51 Mungbean Yield Pods plant-1 Grains pod- Grain index Nodules Grain N content Tissue N content 2011 (kg h-1) (g) plant-1 (g.kg-1) (g.kg-1) Sole 661.58 a 24 a 10 a 41.39 a 19 a 33.54 14.91 Intercrop 14.92 b 3 b 6 b 20.52 b 7 b 27.94 14.09 2012 Sole 671.52 a 25 a 11 a 43.66 a 19 a 33.52 15.40 a Intercrop 16.14 b 3 b 6 b 21.21 b 7 b 29.09 14.43 b Mean Sole 666.55 a 25 a 10 a 42.53 a 19 a 33.53 a 15.16 a Intercrop 15.53 b 3 b 6 b 20.87 b 7 b 28.52 b 14.26 b Within columns in each growing season, means followed by the same letter are not significantly different according to LSD (p =0.05). Means with no letter are not statistically different (F>0.05). Table 3. Yield and yield traits (averaged across nutrients N, P, and Intercropping) of sunflower and mungbean under paired mean comparison as affected by control versus rest (fertilized) during spring 2011 and 2012. Crop & Status Yield Head Diameter Grains 1000 grains Oil content Grain N content Tissue N content Sunflower (kg ha-1) (cm) head-1 weight (g) (%) (g kg-1) (g kg-1) 2011 Un-fertilized 1636 b 13.25 b 833 b 33.42 b 42.41 25.99 b 9.63 Fertilized 2352 a 17.44 a 951 a 47.30 a 41.48 31.68 a 10.77 2012 Un-fertilized 1654 b 13.75 b 843 b 34.30 b 43.70 28.06 b 9.86 Fertilized 2377 a 17.77 a 961 a 47.53 a 41.99 31.82 a 10.91 Mean Un-fertilized 1645 b 13.50 b 838 b 33.86 b 43.05 a 27.03 b 9.75 b Fertilized 2364 a 17.61 a 956 a 47.41 a 41.74 b 31.75 a 10.84 a 2M0u1n1g bean (kYgi.ehlad- 1) Pods plant-1 Grains po Grain index (g) Npoladnutle-1s Grain(g N.k gc-o1)n tent Tissu(ge. Nk gc-o1)n tent Un-fertilized 191.42 b 8 b 7 b 27.58 b 18 a 26.73 b 10.14 b Fertilized 354.57 a 14 a 8 a 31.33 a 12 b 31.18 a 14.98 a 2012 Un-fertilized 198.60 b 8 b 7 b 28.31 b 19 a 26.40 b 11.03 b Fertilized 359.97 a 14 a 9 a 32.89 a 12 b 31.85 a 15.35 a Mean Un-fertilized 195.01 b 8 b 7 b 27.94 b 19 a 26.57 b 10.58 b Fertilized 357.27 a 14 a 9 a 32.11 a 12 b 31.52 a 15.17 a Within columns in each growing season, means followed by the same letter are not significantly different according to LSD (p =0.05). Means with no letter are not statistically different (F>0.05). quantitatively into 100ml volumetric flask and made up to taken in account and vice versa for the sole mungbean the volume by adding distilled water. Twenty ml of digest with intercropping. was distilled in presence of 5 ml 40% NaOH solution to a 5 ml boric acid mixed indicator solution. The distillate was titratedagainst standard 0.01 N HCl. A blank was also run RESULTS and subtracted from readings (Khatoon et al., 2010). Data were subjected to analysis of variance (ANOVA) Grains yield (kg ha-1) according to method described by Steel et al. (1997) and treatment means, where found significant, were Maximum of the possible treatments interactions were separated using least significant difference (LSD) test at non-significant (p<0.05) except the fertilizers N- x P-rates P ≤ 0.05. For mungbean, sole sunflower data were not for yield and yield traits (Table 1). The means (averaged Published by Basic Research Journal of Agricultural Science and Review Khan et al. 151 Table 4. Yield and yield traits (averaged across nutrients P, control and intercrop) of sunflower and mungbean as affected by N-rates during spring 2011 and 2012. Crop & N-rates Yield Head Grains 1000 Grains Oil content Grain N content Tissue N content Sunflower (kg ha-1) Diameter (cm) Head-1 Weight (g) (%) (g kg-1) (g kg-1) 2011 30 2085 c 16.25 c 914 c 45.36 c 42.60 a 28.88 c 9.61 b 60 2389 b 17.49 b 949 b 46.83 b 41.77 a 32.57 b 11.26 a 90 2581 a 18.58 a 991 a 49.70 a 40.08 b 33.59 a 11.44 a 2012 30 2116 c 16.51 c 923 c 45.73 c 43.59 a 28.35 c 9.37 b 60 2403 b 17.75 b 960 b 47.08 b 42.07 b 33.29 b 11.24 a 90 2612 a 19.05 a 1001 a 49.77 a 40.29 c 33.83 a 12.12 a Mean 30 2101 c 16.38 c 919 c 45.55 c 43.10 a 28.61 c 9.49 b 60 2396 b 17.62 b 954 b 46.95 b 41.92 b 32.93 b 11.25 a 90 2596 a 18.81 a 996 a 49.73 a 40.19 c 33.71 a 11.78 a 2011 Yield Pods plant-1 Grains Pod-1 Grain index Nodules Grain N content Tissue N content Mungbean (kg ha-1) (g) plant-1 (g.kg-1) (g.kg-1) 30 283.13 c 13 c 7 c 29.97 b 15 a 23.37 c 13.48 c 60 366.84 b 14 b 8 b 31.29 ab 12 b 33.07 b 14.97 b 90 413.74 a 15 a 9 a 32.74 a 9 c 37.11 a 16.49 a 2012 30 290.54 c 13 c 8 b 31.79 b 15 a 24.31 c 13.80 c 60 374.98 b 14 b 8 b 32.35 b 11 b 33.90 b 15.41 b 90 414.39 a 15 a 9 a 34.55 a 9 c 37.33 a 16.85 a Mean 30 286.83 c 13 c 7 c 30.88 b 15 a 23.84 c 13.64 c 60 370.91 b 14 b 8 b 31.82 b 12 b 33.49 b 15.19 b 90 414.06 a 15 a 9 a 33.64 a 9 c 37.22 a 16.67 a Within columns in each growing season, means followed by the same letter are not significantly different according to LSD (p =0.05). Means with no letter are not statistically different (F>0.05). Table 5. Yield and yield traits (averaged across nutrients N, control and intercropping) of sunflower and mungbean as affected by P-rates during spring 2011 and 2012. Crop & P-rates Yield Head Diameter Grains 1000 grains Oil content Grain N content Tissue N Sunflower (kg ha-1) (cm) head-1 weight (g) (%) (g kg-1) content (g kg-1) 2011 30 2284 c 16.90 b 946 46.25 b 40.95 29.18 c 9.51 c 60 2348 b 17.41 ab 949 47.08 b 41.16 31.58 b 10.43 b 90 2423 a 18.02 a 960 48.56 a 42.34 34.28 a 12.37 a 2012 30 2308 c 17.16 b 954 46.54 b 41.46 29.00 c 9.95 b 60 2376 b 17.44 b 961 47.49 b 41.71 31.68 b 10.52 b 90 2448 a 18.71 a 970 48.55 a 42.80 34.79 a 12.25 a Mean 30 2296 c 17.03 b 950 b 46.40 c 41.21 b 29.09 c 9.73 b 60 2362 b 17.43 b 955 ab 47.29 b 41.44 b 31.63 b 10.47 b 90 2435 a 18.36 a 965 a 48.55 a 42.57 a 34.54 a 12.31 a 2M0u1n1g bean (kYgi ehlad- 1) Pods plant-1 Grains pod-1 Grain index (g) Npoladnutle-1s Grain(g N k gc-o1)n tent Tissu(eg Nkg c-1o)n tent 30 329.98 c 13 c 8 b 30.40 b 11 b 28.78 b 13.99 b 60 357.05 b 14 b 9 a 30.39 b 12 a 30.88 ab 14.63 b 90 376.67 a 15 a 9 a 33.21 a 12.a 33.90 a 16.32 a 2012 30 336.14 c 13 c 8 b 31.71 b 11 b 29.39 b 14.23 b 60 361.96 b 14 b 9 ab 32.11 b 12 a 31.46 ab 14.91 b 90 381.81 a 15 a 9 a 34.86 a 13 a 34.69 a 16.91 a Mean 30 333.06 c 13 c 8 b 31.05 b 11 b 29.09 b 14.11 b 60 359.50 b 14 b 9 ab 31.25 b 12 a 31.17 b 14.77 b 90 379.24 a 15 a 9 a 34.04 a 13 a 34.29 a 16.62 a Within columns in each growing season, means followed by the same letter are not significantly different according to LSD (p =0.05). Means with no letter are not statistically different (F>0.05). across N and P) of cropping systems (CS) i.e. sole vs. independently for main (sunflower) and intercrop intercrop (Table 2 above) and paired means comparison (Mungbean). Grain yield significantly (p<0.05) affected by (averaged across N, P and CS) i.e. control vs. rest the given N-fertilizer rates showing marked increases (fertilized, Table 3) and the applied fertilizer N-rates with the increase N from 30 to 90 kg ha-1 in a year and (Table 4) and P-rates (Table 5) are presented two years averages (Table 4). Similar increases were Published by Basic Research Journal of Agricultural Science and Review 152. Basic Res. J. Agric.Sci. Rev. 3200 22 30 P2O5 30 P2O5 60 P2O5 21 60 P2O5 3000 90 P2O5 90 P2O5 20 -1d (kg ha) 2800 meter (cm) 1189 Grain yiel 2600 Head dia 17 16 2400 15 2200 14 30 60 90 30 60 90 Nitrogen levels (kg ha-1) Nitrogen levels (kg ha-1) 44 45 30 P2O5 60 P2O5 30 P2O5 43 90 P2O5 60 P2O5 40 90 P2O5 42 1) -g Oil content (%) 4401 n N content (g k 3305 ai 39 Gr 25 38 37 20 30 60 90 30 60 90 Nitrogen levels (kg ha-1) Nitrogen levels (kg ha-1) 18 30 P2O5 60 P2O5 90 P2O5 16 1) -g nt (g k 14 e nt o N c 12 e u s Tis 10 8 30 60 90 Nitrogen levels (kg ha-1) Figure 2. Interactive effects of nutrients (Nitrogen and Phosphorous) on grain yield (kg ha-1), head diameter (cm), Oil content (%), grain N-content (%) and tissue N-content (%) of sunflower. Vertical bars shows ±SE of means. observed under the given P-rates in a year or two years show as increase in yield by enhancing N–fertilizer from average basis (Table 5). Sole and intercropped sunflower 30 to 90 kg ha-1 in a dissimilar fashion for the given P- did not show any (p<0.05) change in yield (Table 2). fertilizer rates. Linear marked increases in the yield were Paired mean comparisons showed higher yield in noticed for the given P 30 kg ha-1 for additional N-fertilizer fertilized than unfertilized treatment (Table 2). The only rates. Yield increments at a given P-fertilizer rates (60 interaction N x P (Figure 2) of sunflower was significant to and 90 kg ha-1) were alike with marked (30-60 kg ha-1) to Published by Basic Research Journal of Agricultural Science and Review Khan et al. 153 500 18 30 P2O5 30 P2O5 60 P2O5 17 60 P2O5 450 90 P2O5 90 P2O5 16 -1d (kg ha) 400 -1ber plant 1145 el m Grain yi 350 Pods nu 13 12 300 11 250 10 30 60 90 30 60 90 Nitrogen levels (kg ha-1) Nitrogen levels (kg ha-1) 10.5 38 30 P2O5 30 P2O5 60 P2O5 60 P2O5 10.0 90 P2O5 36 90 P2O5 -1Grains number pod 8899....0505 1000 grains weight (g) 333024 28 7.5 7.0 26 30 60 90 30 60 90 Nitrogen levels (kg ha-1) Nitrogen levels (kg ha-1) 50 30 P2O5 22 45 6900 PP22OO55 20 3600 PP22OO55 1) 40 90 P2O5 -n N content (g kg 3305 -1N content (g kg) 1168 Grai 25 Tissue 14 20 12 15 10 30 60 90 30 60 90 Nitrogen levels (kg ha-1) Nitrogen levels (kg ha-1) Figure 3. Interactive effects of nutrients (Nitrogen and Phosphorous) on grain yield (kg ha-1), pods plant-1, grain number pod-1, thousand grains weight (g), grain N content (%) and tissue N- content (%) of mungbean. Vertical bars show ±SE of mean. moderate (60-90 kg ha-1) changes by increasing N- comparison showed higher (p<0.05) yield in the fertilized fertilizer to the sunflower crop. Mungbean yield also than the control plot. Interactions N x P (Figure 3), N x increased (p<0.05) with increasing N-fertilizer from 30 kg CS (Figure 4), P x CS (Figure 5) and N x P x CS (Figure ha-1 onwards in a year or two years averages (Table 4). 6) were significant for mungbean crop and showed Similarly, an increase in yield was associated to increase increase in yield by increasing N-fertilizer from 30 to 90 P-fertilizer from 30 to 90 kg ha-1 (Table 5). Sole crop kg ha-1 at all three P with marked increase from N 30 to exhibited statistically more yield as compared to the 60 kg ha-1 and moderate thereafter from P 60 to 90 kg ha- intercropped mungbean (Table 2). Paired means 1 with highest for P-fertilizer 90, followed by 60 and lowest Published by Basic Research Journal of Agricultural Science and Review 154. Basic Res. J. Agric.Sci. Rev. 52 1000 Sole crop Sole crop Intercrop 51 Intercrop 800 50 000 grains weight (g) 444789 -1Grain yield (kg ha) 246000000 1 46 0 45 44 30 60 90 30 60 90 Phosphorus levels (kg ha-1) Phosphorus levels (kg ha-1) 35 Sole crop 12 Intercrop Sole crop Intercrop 30 11 25 -1er plant 20 -1ber pod 10 b m 9 m u Pods nu 1105 Grains n 8 7 5 0 6 30 60 90 30 60 90 Phosphorus levels (kg ha-1) Phosphorus levels (kg ha-1) 24 Sole crop Intercrop 22 20 -1nt 18 a er pl 16 b m 14 u n e 12 ul d No 10 8 6 4 30 60 90 Phosphorus levels (kg ha-1) Figure 4. Interactive effects of Phosphorous and Cropping System on grain yield (kg ha-1) of sunflower and grain yield (kg ha-1), pod number plant-1, grain number plant-1, nodules plant-1 of mungbean. Vertical bars show ±SE of means. for P 30 kg ha-1. A negligible increase in intercrop and as sole and intercrop. Increased N-fertilizer from 30 to 90 moderate in sole crop was noticed by increasing N-fertilizer kg ha-1 markedly increase yield in all three P-rates of sole rate from 30 to 90 kg ha-1. Similar, response of interaction P mungbean and linear marked increments in intercrop x CS was noted for yield of sole with a negligible change in mungbean. Increased P-rate showed higher yield at any the intercrop. Interactions of treatments (N x P x CS) given N-fertilizer rate. showed different response for the different P- and N-rates Published by Basic Research Journal of Agricultural Science and Review Khan et al. 155 1000 35 Sole crop Sole crop Intercrop Intercrop 30 800 25 -1ain yield (kg ha) 460000 -1ds number plant 1250 Gr 200 Po 10 0 5 0 30 60 90 30 60 90 Nitrogen levels (kg ha-1) Nitrogen levels (kg ha-1) 14 30 Sole crop Sole crop Intercrop Intercrop 13 25 12 1 -1mber pod 1101 -mber plant 1250 Grains nu 9 odules nu 10 N 8 5 7 6 0 30 60 90 30 60 90 Nitrogen levels (kg ha-1) Nitrogen levels (kg ha-1) Figure 5. Interactive effects of nitrogen and cropping system on grain yield (kg ha-1), pod number plant-1, grain number plant-1, nodules plant-1 of mungbean. Vertical bars show ±SE of means. Yield contributing traits However, increased P-rate from 30 to 60 kg ha-1 did not show any change in grain number but did show an Major yield contributing traits e.g. the sunflower head increment (p<0.05) at P 90 kg ha-1. Grains head-1 did not diameter (cm), grains head-1 and grains weight (g) indicated vary in sole or intercrop. Evidently, fertilized than control significant (p<0.05) differences with treatment N and P- showed more grains head-1. No interaction response of fertilizer rates, sole and intercropping as well as control vs. grain number was significant (p<0.05). Thousand grains rest (fertilized). Head diameter increased with increasing N- weight (g) increased with increasing N from 30 kg ha-1 fertilizer rates from 30 onwards in a year of study or two onwards in a linear fashion. Likewise, a consistent years averages. However, P 30 and 60 kg ha-1 showed increased (p<0.05) observed in grains weight by increasing non-significant (p<0.05) changes with a significant P-rates from 30 kg ha-1 onwards on two years averages. increase by increasing P 90 kg ha-1 in a year or two years The P 30 and 60 kg ha-1 did not differ in a year. average data (Table 5). Head diameter did not vary Thousand grains weight (g) did not vary by planting (p<0.05) for sole and intercrop. Rest (fertilized) as sunflower sol or intercrop with mungbean. Obviously, compared to control (unfertilized) showed better head fertilized plots showed heavier grains. The CS x P diameter. Only N x P (Figure 2) interaction showed a interaction (Figure 4) was significant (p<0.05) by showing linear response for head diameter by increasing N-fertilizer a linear response with moderate (30-60 kg ha-1) to marked rates from N 30 kg ha-1 onwards at all three P-fertilizer rates (60-90 kg ha-1) increase in the intercrop than sole crop. with highest value at P 90 kg ha-1, followed by 60 and 30 kg Pods plant-1 were significantly vary (p<0.05) with N-rate ha-1 by subsequent increasing the N-rates with moderate to showing positive increments by increasing N-fertilizer rate marked linear changes. N-fertilizer rate has significantly from 30 to 90 kg ha-1 in a year or two years averages (p<0.05) increased grains head-1 by increasing N supply (Table 4). Nutrients response was more or less similar for from 30 kg ha-1 onwards in a year or two years averages. P-fertilizer rates on pods plant-1 (Table 5) with highest Published by Basic Research Journal of Agricultural Science and Review

Description:
including a control treatment (no N and P fertilizer) as sole sunflower, mungbean and their . manual thinning 20 DAS (20 cm) in sunflower and 35 . GY = grain yield, HD = head diameter, GPH =grains per head, TGW = 1000 grains weight, OC = Oil content, GNC and reproductive parts (Bakht et al., 201
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