Table Of Content

CONTENTS Announcement Introduction The first one hundred C.A. Fewson (UK) Microbiology: An important discipline in a changing world J. NGesch (Switzerland) FEMS and FEMS Microbiology Letters: Greetings from the American Society for Microbiology J.L. Ingraham (USA) Most-cited papers in FEMS Microbiology journals C.A. Fewson (UK) FEMS: Past, Present and Future What is ahead, FEMS? P.H. Makela (Finland) How it began: A bit of history D.W. Tempest (UK) The Federation of European Microbiological Societies: An Historical Review E.A. Dawes (UK) European Policy for Science and Research Biotechnology research and policy in the European Community: The first decade and a half M.F. Cantley and J. Dreux de Nettancourt (Belgium) The Language of Microbiology The microbiology literature: Languages of publication and their relative citation impact E. Garfield and A. Welljams-Dorof (USA) Encoding Nature: Writing Science in English J. Corbett (UK) Methods Identification and in situ detection of individual bacterial cells R. Amann, W. Ludwig and K.-H. Schleifer (FRG) identification of Staphylococcus epidermidis using a 16S rRNA-directed oligonucleotide probe J. Zakrzewska-Czerwinska, A. Gaszewska-Mastalarz, G. Pulverer and M. Mordarski (Poland, FRG) DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities _ P.A. Rochelle, J.C. Fry, R.J. Parkes and A.J. Weightman (UK) Enzyme-linked immunosorbent assay for the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection P.A. Lambert, S.J. Krikler, R. Patel and S. Parvathan (UK) Observations on the mode of bacterial infection of hens’ eggs J.L. Lock, J. Dolman and R.G. Board (UK) Investigation of microbial growth in vivo: Evaluation of a novel in vivo chamber implant system J.P. Arbuthnott, E.R. Arbuthnott, A.D.J. Arbuthnott, W.J. Pike and A. Cockayne (UK) Kinetics of stem gravitropism in Coprinus cinereus: Determination of presentation time and ‘dosage-response’ relationships using clinostats J.P. Hatton and D. Moore (UK) Experimental conditions may affect reproducibility of the B-galactosidase assay A. Giacomini, V. Corich, F.J. Ollero, A. Squartini and M.P. Nuti (Italy) Genetics and Molecular Biology Molecular biology of Bacillus subtilis cytochromes C. Von Wachenfeldt and L. Hederstedt (Sweden) The new approaches to whole genome analysis of bacteria B.W. Holloway, M.D. Escuadra, A.F. Morgan, R. Saffery and V. Krishnapillai (Australia) Mutations conferring acid sensitivity in the acid-tolerant strains Ahizobium meliloti WSM419 and Rhizobium leguminosarum biovar viciae WSM710 R.P. Tiwari, W.G. Reeve and A.R. Glenn (Australia) Regulation of nitrogen fixation in Azospirillum brasilense Sp7: Involvement of nifA, ginA and ginB gene products Y.Y. Liang, M. De Zamaroczy, F. Arséne, A. Paquelin and C. Elmerich (France) Genome diversity at the serA-linked capsule locus in Escherichia coli G. Boulnois, R. Drake, R. Pearce and |. Roberts (UK) An analysis of the codon usage of Pasteurella haemolytica A1 R.Y.C. Lo (Canada) 125 Molecular genetic studies of a 10.9-kb operon in Escherichia coli for phosphonate uptake and biodegradation B.L. Wanner and W.W. Metcalf (USA) 133 Misreading of the arg/ message in Escherichia coli J. Parker, J. Precup and C. Fu (USA) 141 Possible function of tRNA}%, in regulation of solvent formation in Clostridium acetobutylicum U. Sauer and P. Durre (FRG) 147 Suppressor mutants of the photosynthetically incompetent pufX deletion mutant Rhodobacter capsulatus DRC6(pTL2) T.G. Lilburn and J.T. Beatty (Canada) 155 Small molecule-mediated density-dependent control of gene expression in prokaryotes: Biolumines- cence and the biosynthesis of carbapenem antibiotics P. Williams, N.J. Bainton, S. Swift, S.R. Chhabra, M.K. Winson, G.S.A.B. Stewart, G.P.C. Salmond and B.W. Bycroft (UK) Physiology: Energetics, Growth and Death Physiological and energetic aspects of bacterial metabolite overproduction D.W. Tempest and O.M. Neijssel (UK, Netherlands) 169 Generalized qualitative treatment of transition states and stability in a chemostat C.J.E.A. Bulder (Netherlands) 177 Expression of periplasmic binding proteins for peptide transport is subject to negative regulation by phosphate limitation in Escherichia coli M.W. Smith and J.W. Payne (UK) 183 TonB-independent ferrioxamine B-mediated iron transport in Escherichia coli K12 M. Nelson and P.J. Szaniszio (USA) 191 Metals and microorganisms: A problem of definition G.M. Gadd (UK) 197 Low temperature induced non-culturability and killing of Vibrio vulnificus D. Weichart, J.D. Oliver and S. Kjelleberg (Sweden, USA) 205 The mechanism of action of chlorhexidine T. Kuyyakanond and L.B. Quesnel (UK) 211 Utilisation of glycerol and glycerol 3-phosphate is differently affected by the phosphotransferase system in Bacillus subtilis L. Beijer and L. Rutberg (Sweden) 217 Energy conservation in fermentative glutarate degradation by the bacterial strain WoGI3 C. Matthies and B. Schink (FRG) 221 The effect of iron limitation on expression of the aerobic and anaerobic electron transport pathway genes in Escherichia coli P.A. Cotter, S. Darie and R.P. Gunsalus (USA) 227 Biochemistry: Enzymes and Metabolism Incompatibility of outer membrane proteins OmpA and OmpF of Escherichia coli with secretion in Bacillus subtilis: Fusions with secretable peptides M. Simonen, E. Tarkka, R. Puohiniemi and M. Sarvas (Finland) 233 Construction and properties of pyruvate dehydrogenase complexes with up to nine lipoyl domains per lipoate acetyltransferase chain R.S. Machado, D.P. Clark and J.R. Guest (UK, USA) 243 Lipase of Staphylococcus hyicus: Analysis of the catalytic triad by site-directed mutagenesis S. Jager, G. Demieitner and F. Gotz (FRG) 249 Evidence for an alternative nitrogenase in Heliobacterium gestii L.K. Kimble and M.T. Madigan (USA) 255 The autolytic (‘suicidase’) system of Enterococcus hirae: From lysine depletion autolysis to bio- chemical and molecular studies of the two muramidases of Enterococcus hirae ATCC 9790 G.D. Shockman (USA) 261 Carbon metabolism in Chioroflexus aurantiacus E.N. Kondratieva, R.N. lvanovsky and E.N. Krasilnikova (Russia) 269 Specific removal of chlorine from the ortho-position of halogenated benzoic acids by reductive dechlorination in anaerobic enrichment cultures J. Gerritse, B.J. Van der Woude and J.C. Gottschal (Netherlands) 273 Growth temperature-dependent activity of glycerol dehydratase in Escherichia coli expressing the Citrobacter freundii dha reguion R. Daniel and G. Gottschalk (FRG) Molecular Mechanisms in Medical Microbiology: Pathogenicity and Virulence The sialylation of gonococcal lipopolysaccharide by host factors: A major impact on pathogenicity H. Smith, J.A. Cole and N.J. Parsons (UK) Inhibitors of lipopolysaccharide biosynthesis impair the virulence potential of Escherichia coli S.M. Hammond (italy) Serratia marcescens forms a new type of cytolysin V. Braun, S. Hobbie and R. Ondraczek (FRG) Penetration of fimbriate enteric bacteria through basement membranes: A hypothesis T.K. Korhonen, R. Virkola, K. Lahteenmaki, Y. Bjorkman, M. Kukkonen, T. Raunio, A.-M. Tarkka- nen and B. Westerlund (Finland) Erythrogenic toxins A, B and C: Occurrence of the genes and exotoxin formation from clinical Streptococcus pyogenes strains associated with streptococcal toxic shock-like syndrome W. Reichardt, H. Miller-Alouf, J.E. Alouf and W. Kéhler (FRG, France) Mucoid Pseudomonas aeruginosa and cystic fibrosis: The role of mutations in muc loci J.R.W. Govan, D.W. Martin and V.P. Deretic (UK, USA) Bacterial flagellar diversity and significance in pathogenesis C.W. Penn and C.J. Luke (UK) Cytotoxic fungal spores in the indoor atmosphere of the damp domestic environment J.E. Smith, J.G. Anderson, C.W. Lewis and Y.M. Murad (UK) Transformation-mediated exchange of virulence determinants by co-cultivation of pathogenic Neisseriae M. Frosch and T.F. Meyer (FRG) Development, Structure and Function Growth and morphogenesis in Streptomyces E.M. Miguélez, C. Martin, M.B. Manzanal and C. Hardisson (Spain) Chromatins of low-protein content: Special features of their compaction and condensation E. Kellenberger and B. Arnold-Schulz-Gahmen (Switzerland) Filamentous Fungi and Yeasts Some controls on oligosaccharide utilization by yeasts: The physiological basis of the Kluyver effect J.A. Barnett (UK) Stress management: Filamentous fungi as exemplary survivors P. Markham (UK) What are the roles of chitinases in the growing fungus? G.W. Gooday, W.-Y. Zhu and R.W. O’Donnell (UK) Hansenula polymorpha: An attractive model organism for molecular studies of peroxisome bio- genesis and function M. Veenhuis, |.J. Van der Klei, V. Titorenko and W. Harder (Netherlands, FRG) Chitin pleomorphism in the cellulosic cell wall fungus Saprolegnia L. Gay, V. Bulone, V. Girard, M. Févre and H. Chanzy (France) Protozoa On the post-translational modifications at the C-terminal domain of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi J.J. Cazzulo, J. Martinez, A.J.A. Parodi, C. Wernstedt and U. Hellman (Argentina, Sweden) interesting and Unusual Organisms A long trail of serendipity-directed research on photosynthetic bacteria H. Gest (USA) Peculiar properties of mycoplasmas: The smallest self-replicating prokaryotes S. Razin (Israel) Bacteriophage and Animal Viruses The lytic enzyme in bacteriophage jM1-induced lysates of Methanobacterium thermoautotrophicum Marburg D. Stax, R. Hermann, R. Falchetto and T. Leisinger (Switzerland) Structural analysis and biological significance of the cell wall lytic enzymes of Streptococcus pneumoniae and its bacteriophage R. L6épez, J.L. Garcia, E. Garcia, C. Ronda and P. Garcia (Spain) Epstein-Barr virus-transformed B lymphocytes produce low molecular mass molecules with auto- crine growth factor and competence factor activity C. Garzelli, A. Bazzichi, A.M. Dayah, M. Manunta, M. Incaprera and G. Falcone (Italy) Why do human hepatitis viruses replicate so poorly in cell cultures? S.M. Lemon, L. Whetter, K.H. Chang and E.A. Brown (USA) 455 Plasmids Diversity of Pseudomonas plasmids: To what extent? A.M. Boronin (Russia) 461 Immunology; Vaccines Hazards of vaccine production A.J. Beale (UK) 469 Some unanswered questions about vaccination F. Brown (USA) 475 How can peptide vaccines work? D.J. Rowlands (UK) 479 The conformational specificity of viral epitopes M.H.V. Van Regenmortel (France) 483 Adjuvanted oral vaccines should not induce allergic responses to dietary antigens D.E.S. Stewart-Tull and A.C. Jones (UK) 489 Systematics Udeniomyces, a new ballistosporous anamorphic yeast genus in the Cryptococcaceae proposed for three Bullera species which produce large bilaterally symmetrical ballistospores T. Nakase and A. Takematsu (Japan) 497 Value of chemosystematic data for predicting anamorph-teleomorph relationships between the genera Rhodotorula and Rhodosporidium K. Komagata (Japan) 503 Population Genetics Variation in O-antigens, niche-specific selection and bacterial populations P.R. Reeves (Australia) 509 Ecology Model of nitrification and denitrification in marine sediments T.H. Blackburn and N.D. Blackburn (Denmark) 517 Biotechnology Cellulose degradation by Clostridium thermocellum: From manure to molecular biology P. Béguin, J. Millet and J.-P. Aubert (France) 523 Rumen microbiology, biotechnology and ruminant nutrition: The application of research findings to a complex microbial ecosystem R.J. Wallace (UK) 529 Author Index Volume 100 Subject Index Volume 100 Notes to Authors Announcements