EZ:faast™ Amino Acid Analysis - Phenomenex

88 Pages·2007·1.19 MB·English

EZ:faast™ Amino Acid Analysis
Theory and practical overview
Presentation Outline
• Why analyze?
• Older Techniques and Limitations
• EZ:faast advantages
• EZ:faast product overview
• EZ:faast applications
• Conclusion
Why Analyze?
• Why analyze amino acids?
– Determination of metabolic disorders
– Fermentation/cell culture analysis
– Nutritional labeling/ feed analysis
– Quality control of foods/beverage
– Protein identification/characterization
– Peptide quality control
Older Techniques
Historical Background and Method
Limitations
Older Techniques
• Past advancements
– IEX with post-column derivatization introduced in 1950s
– Achievements made in
• Derivatization & detection
• Separation science (chromatography media)
Older Techniques
• Derivatization advancements
– Pre-Column derivitization increase sensitivity
– Attachment of chromaphore allows for UV or
florescence detection
Older Techniques
• Separation science advancements
– Chromatography media improvements increased
resolution
• Ion exchange medias (post-column derivatization)
• Reversed phase medias (pre-column derivatization)
Older Techniques
• Ion exchange with post-column derivatization
– Derivatizing agents
• Ninhydrin or OPA
– Products
• Beckman® 6300, Pickering® Labs, Hitachi® L880,
Biochrom® 30 series
– Strengths
• Established methods
• Chemistry well characterized
Older Techniques
• Reversed phase with pre-column derivatization
– Derivatizing agents
• ACQ, OPA, PITC, FMOC
– Products
• Waters® Pico-Tag® & AccQ-Tag™
– Strengths
• Faster analysis times / improved throughput
• More sensitive
Older Techniques
• Ion chromatography with electrochemical detection
– Products
• Dionex® AAA Direct®
– Strengths
• No derivatization necessary
• Compatible with gel hydrolysis