Experimental Hepatocarcinogenesis PDF
Preview Experimental Hepatocarcinogenesis
Experimental Hepa tocarcinogenesis Experimental Hepa tocarcinogenesis Edited by M. B. Roberfroid and V. Preat Universite Catholique de Louvain Brussels, Belgium Plenum Press • New York and London Library of Congress Cataloging in Publication Data European Meeting on Experimental Hepatocarcinogenesis (2nd: 1987: Spa, Belgium) Experimental hepatocarcinogenesis. "Proceedings of a European Meeting on Experimental Hepatocarcinogenesis, held May 27-30, 1987, in Spa, Belgium" -T. p. verso. Includes bibliographies and index. 1. Liver-Cancer-Congresses. 2. Carcinogenesis-Congresses. 3. Liver-Cancer Animal models-Congresses. 4. Oncology, Experimental-Congresses. I. Roberfroid, M. B. II. Preat, V. III. Title. [DNLM: Liver Neoplasms, Experimental-Congresses. W3 EU886F 2nd 1987e 1 WI 735 E887 1987el RC280.L5E97 1987 616.99'436 87-35725 ISBN-13: 978-1-4612-8264-8 e-ISBN-I3: 978-1-4613-0957-4 DOl: 10.1007/978-1-4613-0957-4 Proceedings of a European Meeting on Experimental Hepatocarcinogenesis, held May 27-30,1987, in Spa, Belgium © 1988 Plenum Press, New York A Division of Plenum Publishing Corporation 233 Spring Street, New York, N.Y. 10013 Sof'tcover reprint of the hardcover 1st edition 1988 All rights reserved No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher PREFACE The meeting on experimental hepatocarcinogenesis which took place in Spa, Belgium at the end of May 1987 was the Second European Meeting. About 100 scientists, mostly from Europe but also from the United States, met there for three days in a very friendly atmosphere to exchange knowledge and ideas on experimental and human liver carcinogenesis. The main topics discussed during the meeting included general reviews on hepatocarcinogenesis, experimental models of hepa tocarcinogenesis, biology of hepatocarcinogenesis, and in vitro studies in hepatocarcinogenesis. They are all covered by the various chapters of this proceedings volume, which reflects the present state of knowledge in this important field of cancer research. The final aim of that research is to understand the basic mechanisms of carcinogenesis. The liver offers a parti cularly interesting tool to reach such a goal. Indeed, its biochemistry, its morphology, and its physiology are very diverse, but relatively well known. Various protocols have been developed to produce hepatocellular carcinomas or other malignant tumors. Their appearance is most often preceded by phenotypically altered foci and nodules which have been isolated and characterized. The major cell populations of normal, neoplastic, and malignant livers have been cultivated. Once again the major conclusion of the meeting was the complexity of the carcinogenic process. More than ever, it has become clear that understanding the carcinogenic process will require collecting and integrating information and discoveries from different disciplines. Even though we each have to speci alize in order to obtain the most detailed and complete infor mation possible, at the same time, we have to be very open-minded and try to understand each other's results. No one single discipline alone will give the explanation we are looking for. The cancer problem is at the heart of the major biological problems, it involves all the complexity of life. Keeping that in mind, all the participants helped create during the meeting, an atmosphere of modesty and since rity, which made all discussion very fruitful and highly v constructive. Various projects of mutual exchanges and colla boration were discussed, which are the best guarantees for further progress. We hope that greater advances will be presented at the next meeting, which is expected to take place in the near future. Thanks to our Hungarian colleagues, who have made a very attractive offer to organize the Third European Meeting on Hepatocarcinogenesis in their country. On behalf of the international organizing committee, I invite you to join us in 1990. Prof. M. Roberfroid CONTENTS Preface ............................................... 1 EXPERIMENTAL AND HUMAN LIVER CARCINOGENESIS CAUSE AND MODULATION Hepatocarcinogens 5 H. Neuman The Induction of Localized Tumors by Carcinogens Implanted into the Liver ......................... 15 K. Aterman Two Stage Theory of Carcinogenesis : a critical review Introducing the Concept of Modulation 29 M. Roberfroid Modulating Factors of Hepatocarcinogenesis 41 V. Preat and N. Delzenne Sex Hormones as Modulators of Liver Tumor Development 51 S.D. Vesselinovitch, N. Mihailovich and S. Negri Liver Cancer and Viruses .............................. 63 Z. Schaff and K. Lapis MORPHOLOGICAL ALTERATIONS DURING LIVER CARCINOGENESIS Metabolic Zonation of the Liver ....................... 79 K. Jungermann Cellular Differentiation during Neoplastic Develop- ment in the Liver ................................ 89 P. Bannasch, H. Enzmann, Y. Ruan, E. Weber and H. Zerban Histochemical Analysis of Hepatocarcinogenesis ........ 105 H.J. Hacker, G. Seelman-Eggebert, F. Klimek, P. Peschke and R. Kletzien CELL PROLIFERATION, CELL DEATH AND LIVER CARCINOGENESIS Cell Proliferation and Hepatocarcinogenesis 121 H.M. Rabes vii Initiation of Chemical Hepatocarcinogenesis : Compen satory Cell Proliferation versus Mitogen Induced Hyperplasia •.•.....•..•......•..........•....... 133 A. Columbano, G.M. Ledda-Columbano, P. Coni, D.S.R. Sarma and P. Pani Cell Death (Apoptosis) in Normal and Preneoplastic Liver Tissue ........•.•...........•................... 143 W. Bursch and R. Schulte-Hermann BIOCHEMICAL ALTERATIONS DURING LIVER CARCINOGENESIS A Study of the Activities of Carbohydrate Metabo lizing. Enzymes and the Levels of Carbohydrate Metabolites and Amino Acids in Normal Liver and in Hepatocellular Carcinoma ............... 163 V. Gerbracht, E. Roth, K. Becker, M. Reinacher and E. Eigenbrodt The Expression of Cell Surface Receptors in Regene- rating and Neoplastic Liver Tissue ...........••.. 175 L. Eriksson, P. Rissler, N. Andersson, Ch. Moller, G. Norstedt and G. Andersson Cholesterol Metabolism during Cell Proliferation ...•.. 185 P. Pani, S. Dessi, B. Batetta Mechanism of the Inhibition of Liver Carcinogenesis Promotion by S-adenosy1-L-methionine ............. 195 F. Feo, R. G~rcea, L. Daino and R. Pascale NUCLEAR AND GENETIC ALTERATIONS DURING LIVER CARCINOGENESIS Long Term Effect of Diethylnitrosamine (DEN) on the Production of Micronuclei in Precancerous Rat Liver .....•....................•••.............•• 211 H. Barbason, B. Bouzahzah, S. Massart, D. Brumioul, B. Robaye and Ch. Herens Nuclear Alterations during Hepatocarcinogenesis Promotion by 2-acetylaminofluorene ...........•... 221 P. Seglen, G. Saeter and P. Schwarze Cytogenetic and Genetic Alterations during Hepato- carcinogenesis .............•••................•.. 231 M. Kirsch-VoIders, S. Haesen, A. Deleener, Ph. Cas telain, M. Alexandre and V. Preat Role of Oncogenes in Hepatocarcinogenesis ..•.......••. 245 C. Guguen-GuiIbuzo, G. Baffet, P.L. Etienne, D. Glaise, N. Defer, M. Corral, D. Corcos and J. Kruh IN VITRO STUDIES IN LIVER CARCINOGENESIS Separation and Biochemical Characterization of Rat Liver Parenchymal Cell Subpopulations ............ 257 P. Steinberg, B. Seibert and F. Oesch viii Growth Control of Hepatocytes, their Immortalization and Transformation by Transforming Genes of Polyoma Virus and of SV40 Virus •••••••••••.•••••• 267 D. Paul Changes in Hepatocyte TGFB Receptors and Gene Expres- sion during Normal and Neoplastic Liver Growth •.. 275 B.L. Carr, R.H. Whitson and K. Itakura Regulation of Carbohydrate Metabolism in a Glycogen storing Liver Cell Line •................•.•••••.. 283 D. Mayer Techniques in Measuring DNA Synthesis and Mitosis Induced by Tumor Promoters in Hepatocytes Primary Cultu- res •.•.•..•••.••.•.••..•••••.••..•.••.•.••••••.•. 297 W. Parzefall and F.A. Puhringer CONCLUSIONS Concluding Remarks ••....•.....•..••.•..•........•..••• 309 R. Schulte Hermann Contributors ..•...•.•..•..••.•..••.•.••••••••••••.••.• 313 Index ....•..•.••.•.........•...•.....•.•....•......••• 317 ix INTRODUCTION EXPERIMENTAL AND HUMAN LIVER CARCINOGENESIS CAUSE AND MODULATION
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