EVALUATION OF BIOCONTROL AGENTS AGAINST COLLAR ROT PATHOGEN (Sclerotium rolfsii ...

148 Pages·2016·5.5 MB·English

EVALUATION OF BIOCONTROL AGENTS AGAINST
COLLAR ROT PATHOGEN (Sclerotium rolfsii Sacc.) OF
BRINJAL (Solanum melongena L.)

By

Mr. B. ANILKUMAR

B. Sc. (Ag.)

THESIS SUBMITTED TO THE
ACHARYA N.G.RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE

DEPARTMENT OF PLANT PATHOLOGY
COLLEGE OF AGRICULTURE,
ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
RAJENDRANAGAR, HYDERABAD-500 030, ANDHRA PRADESH
INDIA

2008
CERTIFICATE

Mr. B. ANILKUMAR has satisfactorily prosecuted the course of research
and that the thesis entitled “EVALUATION OF BIOCONTROL AGENTS
AGAINST COLLAR ROT PATHOGEN (Sclerotium rolfsii Sacc.) OF BRINJAL
(Solanum melongena L.)” submitted, is the result of original research work and is of
sufficiently high standard to warrant its presentation to the examination. I also certify
that the thesis or part thereof has not been previously submitted by him for a degree of
any University.

Date : (Dr. V. KRISHNA RAO)
Place : Hyderabad Chairman of the Advisory Committee
CERTIFICATE

This is to certify that the thesis entitled “EVALUATION OF
BIOCONTROL AGENTS AGAINST COLLAR ROT PATHOGEN (Sclerotium
rolfsii Sacc.) OF BRINJAL (Solanum melongena L.)” submitted in partial
fulfilment of the requirements for the degree of “MASTER OF SCIENCE IN
AGRICULTURE” for Acharya N. G. Ranga Agricultural University, Hyderabad,
is a record of the bonafide research work carried out by Mr. B. ANILKUMAR
under my guidance and supervision. The subject of the thesis has been approved by
the Student's Advisory Committee.

No part of the thesis has been submitted for any other degree or diploma. The
published part has been fully acknowledged. All the assistance and help received
during the course of the investigation have been duly acknowledged by the author of
the thesis.

(Dr. V. KRISHNA RAO)
Chairman of the Advisory Committee

Thesis approved by the Student's Advisory Committee.

Chairman : (Dr. V. KRISHNA RAO) ___________________________
Professor
Department of Plant Pathology,
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030.

Member : (Dr. T. NARSI REDDY) __________________________
Professor and Head
Department of Plant Pathology,
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030.

Member : (Dr. A. SIVA SANKAR) ___________________________
Professor
Department of Plant Physiology,
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030.
DECLARATION

I, Mr. B. ANILKUMAR hereby declare that the thesis entitled
“EVALUATION OF BIOCONTROL AGENTS AGAINST COLLAR ROT
PATHOGEN (Sclerotium rolfsii Sacc.) OF BRINJAL (Solanum melongena L.)”
submitted to Acharya N. G. Ranga Agricultural University for the Degree of
MASTER OF SCIENCE IN AGRICULTURE is a result of original research work
done by me. It is further declared that the thesis or any part there of has not been
published earlier in any manner.

Date : (B. ANILKUMAR)
Place : Hyderabad
CONTENTS

CHAPTER TITLE PAGE NO.
NO.

I INTRODUCTION

II REVIEW OF LITERATURE

III MATERIALS AND METHODS

IV RESULTS

V DISCUSSION

VI SUMMARY

LITERATURE CITED
LIST OF TABLES
Table Page
Title
No. No.
1 Major symptoms of various diseases caused by Sclerotium rolfsii on
different crops
2 Details of native and commercial biocontrol agents
3 Details of Botanicals
4 Details of Botanicals concentrations
5 Details of Fungicides
6 List of antagonistic mycoflora isolated from rhizosphere and
rhizoplane of forest plants from Warangal district
7 Efficacy of native and commercial fungal antagonists against
S. rolfsii
8 Efficacy and inhibition zone of bacterial antagonists against
S. rolfsii
9 Efficacy of botanicals on mycelial growth of S. rolfsii
10 Compatibility of fungal biocontrol agents with fungicides
11 Compatibility of bacterial biocontrol agents with fungicides
12 Compatibility of fungal biocontrol agents with botanicals
13 Compatibility of bacterial biocontrol agents with botanicals
14 Effect of different fungitoxicants on the incidence of collar rot of
brinjal in pot culture
15 Effect of different fungitoxicants on plant height in pot culture
16 Effect of different fungitoxicants on dry weights of brinjal plants
17 Final population of S. rolfsii in different treatments recorded 30 days
after transplanting
LIST OF ILLUSTRATIONS

Figure Title Page
No. No.
1 Efficacy of native and commercial fungal antagonists against
S. rolfsii
2 Efficacy and inhibition zone of bacterial antagonists against
S. rolfsii
3 Efficacy of botanicals on mycelial growth of S. rolfsii
4 Compatibility of fungal biocontrol agents with fungicides
5 Compatibility of fungal biocontrol agents with botanicals
6 Compatibility of bacterial biocontrol agents with fungicides
7 Compatibility of bacterial biocontrol agents with botanicals
8 Effect of different fungitoxicants on the incidence of collar rot of
brinjal in pot culture
9 Effect of different fungitoxicants on plant height in pot culture
10 Effect of different fungitoxicants on dry weights of brinjal plants
11 Final population of S. rolfsii in different treatments recorded 30
days after transplanting
LIST OF PLATES

Plate Title Page
No. No.
1 Mass multiplication of Sclerotium rolfsii on sorghum grains
2 Mass multiplication of commercial Trichoderma viride
(Bhoomika) on sorghum grains
3 Whitish mycelial growth of Sclerotium rolfsii at collar region of
brinjal
4 Pure culture of Sclerotium rolfsii with sclerotial bodies maintained
on potato dextrose agar medium
5 Photomicrograph of the Sclerotium rolfsii
6 Photomicrograph of the sclerotial bodies of Sclerotium rolfsii
7 Pure cultures of different mycoflora isolated from forest plants
8 Photomicrograph of Trichoderma viride
9 Photomicrograph of Trichoderma sp 2
10 Photomicrograph of Aspergillus niger showing conidial head
11 Pure cultures of commercial Trichoderma viride maintained on
potato dextrose agar medium
12 Antagonism of Trichoderma sp 1, Trichoderma sp 2 (Commercial
and native) and Aspergillus niger against Sclerotium rolfsii
13 Pure culture of Pseudomonas fluorescens maintained on
Pseudomonas Agar medium
14 Pure culture of Bacillus subtilis maintained on Nutrient agar
medium in slants
15 Effect of Pseudomonas fluorescens 1, commercial Pseudomonas
fluorescens and Bacillus subtilis on the mycelial growth of
Sclerotium rolfsii
16 Interaction of commercial Trichoderma viride (Bhoomika) with
Sclerotium rolfsii
17 Interaction of commercial Trichoderma viride (Bhoomika)
with Sclerotium rolfsii)
18 Interaction of native Trichoderma sp 2 with Sclerotium rolfsii
19 Botanicals (Garlic bulb extract and neem oil) used in present
studies
20 Efficacy of neem oil on the mycelial growth of Sclerotium rolfsii
21 Efficacy of Garlic bulb extract on the mycelial growth of
Sclerotium rolfsii
22 Commercial fungicides used in the present studies
23 Compatibility of commercial Trichoderma viride (Bhoomika) and
native Trichoderma sp 2 with fungicide (Copper oxychloride) at
recommended and half recommended dosages
24 Compatibility of commercial Trichoderma viride 1 (Bhoomika)
and native Trichoderma sp 2 with fungicide (Thiram) at
recommended and half recommended dosages
25 Compatibility of commercial Trichoderma viride (Bhoomika) and
native Trichoderma sp 2 with neem oil at recommended and half
recommended dosages
26 Compatibility of commercial Trichoderma viride (Bhoomika) and
native Trichoderma sp 2 with Garlic bulb extract at recommended
and half recommended dosages
27 Layout of in vivo experiment in glass house condition. Effect of
different treatments on the incidence of collar rot of brinjal (cv
Bhagyamati)
28 Effect of treatment T on per cent collar rot incidence of brinjal
15
(cv Bhagyamati)
29 Effect of treatment T on per cent collar rot incidence of brinjal (cv
4
Bhagyamati)
ACKNOWLEDGEMENTS
Glory to be Lord Jesus in the highest for His unfailing love. No book of His
size can be completed without the help of many individuals. Hence, thanks and
gratitude are owed to all of them for their help in various ways in completion of His
volume.
I have no other satisfaction than to express my deep sense of gratitude to my
Chairman, the indefatigable person, Dr. V. Krishna Rao, Professor, Department of
Plant Pathology, College of Agriculture, Rajendranagar, Hyderabad and Chairman
of my Advisory Committee, with his interest, dedication and empathy had devoted his
valuable time patiently to mend my thinking from conception to completion of this
task. I have no qualms in admitting that his logical, critical and methodological
approach has ignited me to perform and excel. The abiding interest, sumptuous
suggestions, timely advices, deliberate counsel during course of investigation enable
the research successfully and shape the thesis in the elegant form. I feel it a great
privilege to work with him and I am deeply indebted to him.
I wish to take this valuable opportunity to express my deep sense of gratitude
and profound thanks to Dr. T. Narsi Reddy, Professor and Head, Department of
Plant Pathology, College of Agriculture, Rajendranagar, Hyderabad and member of
my Advisory Committee for his constant help and valuable suggestions and
encouragement during the course of my study and prosecution of research work.
I am greatly indebted to Dr. A. Siva Sankar, Professor, Department of Plant
Physiology, College of Agriculture, Rajendranagar, Hyderabad and member of my
advisory committee for providing concrete ideas and valuable guidance during the
pursuit of my work and extended cooperation in the completion of the thesis.
It gives me great pleasure to express my deep sense of gratitude to
Dr. D. Raja Ram Reddy, Associate Director of Research, Regional Agricultural
Research Station, Palem for his valuable suggestions and kindness during my study
and prosecution of research work.
I avail this opportunity to place on record my respect and gratitude to
Dr. P. Narayana Reddy, Professor, Department of Plant Pathology, College of