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Evaluating inorganic nanoparticles in the living organism Caenorhabditis elegans Laura González PDF

329 Pages·2017·14.5 MB·English
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Preview Evaluating inorganic nanoparticles in the living organism Caenorhabditis elegans Laura González

ADVERTIMENT. Lʼaccés als continguts dʼaquesta tesi queda condicionat a lʼacceptació de les condicions dʼús establertes per la següent llicència Creative Commons: http://cat.creativecommons.org/?page_id=184 ADVERTENCIA. El acceso a los contenidos de esta tesis queda condicionado a la aceptación de las condiciones de uso establecidas por la siguiente licencia Creative Commons: http://es.creativecommons.org/blog/licencias/ WARNING. The access to the contents of this doctoral thesis it is limited to the acceptance of the use conditions set by the following Creative Commons license: https://creativecommons.org/licenses/?lang=en Evaluating inorganic nanoparticles in the living organism Caenorhabditis elegans Laura González Moragas DOCTORAL THESIS Doctoral Studies in Materials Science Directors: Dr. Anna Roig and Dr. Anna Laromaine Tutor: Dr. Gemma Garcia Departament de Física, Facultat de Ciències 2016 Dra. Anna Roig Serra, Investigadora Científica del CSIC, la Dra. Anna Laromaine Sagué, Investigadora Científica del CSIC, i la Dra. Gemma Garcia Alonso, Professora de la UAB CERTIFIQUEN: Que la Laura González Moragas, Graduada en Farmàcia per la Universitat de Barcelona, ha dut a terme aquesta tesis doctoral sota la seva direcció I que porta per títol “Evaluating inorganic nanoparticles in the living organism Caenorhabditis elegans”, la qual queda recollida en aquesta memòria per optar al grau de Doctor en Ciència en el Programa de Ciència de Materials amb Menció Internacional. I perquè així consti, signen el present certificat Dra. Anna Roig Dra. Anna Laromaine Dra. Gemma Garcia Bellaterra, 19 de Setembre 2016 Als meus pares Elisabet i Raimundo i al meu estimat marit Alfonso. “Equipped with his five senses, man explores the universe around him and calls the adventure Science.” Edwin Powell Hubble, American astronomer Acknowledgements Before 2012, the year I graduated in Pharmacy at the Universitat de Barcelona, I was not aware of the possibility of pursuing a PhD. Pharmacy has a long list of “appealing” job positions to apply for: in pharmacies, hospitals, laboratories, “big pharma” companies, etc. I guess this is the reason why we were not very encouraged to pursue any further studies other than Master’s degrees. During my research into ‘what next?’ after my degree, I fulfilled my desire of working in the pharmaceutical industry first as an intern and then securing a job position, but I did never meet my desire of researching into biomedicine, which was actually my very initial motivation for enrolling in Pharmacy. Hence, I decided to look for a PhD position in a field that complemented my knowledge on health sciences with a more technical focus. Nanotechnology seemed to meet all the requirements. I contacted several groups and was really keen on Dr. Anna Roig’s group from the very beginning. The project that Anna Roig and Anna Laromaine proposed sounded very exciting and challenging, the perfect combination between biology and materials science that I was looking for. Moreover, I was really impressed by their CVs and international experience. I really want to thank both Annas for their great support since I first contacted them and throughout all this time. Their balance of patience, ambition, focus on quality and orientation to results helped me to progress in my research and also in my personal life. I also have to thank previous colleagues from ICMAB who I really miss these days and who I shared many memorable moments with: Elisa, Muling, Maria, Nerea, Wojtek, Pengfei, Siming, Rafa, Raluca, Elizabeth, Dejan, Laura; and my current ICMAB colleagues: Anna May, Roxana, Cica, Cristiano, Mengdi, Jike, Qhianze, Ivan, Deyaa... I would like to thank our collaborators: Dr. Yannick Schwab from EMBL and his great Electron Microscopy team; Dr. Stephen Stürzenbaum from KCL and the kind members of the Toxicogenomics group, who welcomed and helped me during a 3-month stay in his lab; Dr. Romain Quidant and his group at ICFO for their collaboration and support; Dr. Jesús de la Fuente and his group at INA-UNIZAR for their interest in the hyperthermia experiments in C. elegans; and Dr. Lorenzo Albertazzi from IBEC for his motivation to combine our expertise. I also thank all the other ‘worm’ labs with who we have been in touch during these years (Cerón lab from IDIBELL, Marta Artal lab from CABD, Natascia Ventura lab from IUF, Juan Cabello lab from CIBIR, Esther Dalfo lab from UAB...). I would also like to acknowledge my previous colleagues and bosses from Laboratorios Salvat, especially Albert, Lourdes, Xavier and Daniel. They advised me to leave the company and undertake a PhD so I could progress in my professional career. Their support comforted me when I still had some doubts about whether to go for the PhD adventure or to stay in the industry. i

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