Epidemiological, immunological and virological aspects of acute and chronic hepatitis C v

192 Pages·2017·7.4 MB·English

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Epidemiological, immunological and virological aspects of acute and chronic
hepatitis C virus infections
Thomas, X.V.
Publication date
2015
Document Version
Final published version
Link to publication
Citation for published version (APA):
Thomas, X. V. (2015). Epidemiological, immunological and virological aspects of acute and
chronic hepatitis C virus infections. [Thesis, fully internal, Universiteit van Amsterdam].
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Download date:18 Jan 2023
Epidemiological, Immunological and Virological
Aspects of Acute and Chronic
Xiomara Varissa Thomas
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Epidemiological, immunological and virological
aspects of acute and chronic hepatitis C virus infections

Xiomara Varissa Thomas
ISBN: 978-94-91602-33-7
Layout by Xiomara V. Thomas
Cover design made by Timothy Heymans (Heymans advertising)
Printed by Print service Ede

The printing of this thesis book was financially supported by:
Academic Medical Center

Copyright © 2015 by Xiomara V. Thomas
All rights reserved. No part of this publication may be reproduced, stored or transmitted in any way
without permission from the author. Published papers were reprinted with permission from the
publisher.
Epidemiological, Immunological and Virological
Aspects of Acute and Chronic Hepatitis C Virus Infections

ACADEMISCH PROEFSCHRIFT

Ter verkrijging van de graad van doctor
aan de Universiteit van Amsterdam
op gezag van de Rector Magnificus
prof. dr. D.C. van den Boom
ten overstaan van een door het college voor promoties ingestelde
commissie, in het openbaar te verdedigen in de Agnietenkapel

Op vrijdag 13 maart 2015, te 12:00 uur

door

Xiomara Varissa Thomas

geboren te

Paramaribo, Suriname
Promotor: Prof. dr. M.D. de Jong
Copromotores: Dr. C.J. Schinkel
Dr. R. Molenkamp

Overige leden: Prof. dr. B. Berkhout
Prof. dr. M.P.G. Koopmans
Prof. dr. U.H.W. Beuers
Prof. dr. H.L. Zaaijer
Dr. K.C. Wolthers
Dr. P.A. Boonstra

Faculteit der Geneeskunde

The research described in this thesis was performed at the Department of Medical Microbiology, section
of clinical virology, Academic Medical Center, University of Amsterdam , Amsterdam, The Netherlands.
“Science investigates; religion interprets. Science gives man
knowledge, which is power; religion gives man wisdom, which
is control. Science deals mainly with facts; religion deals
mainly with values. The two are not rivals. They are
complementary. Science keeps religion from sinking into the
valley of crippling irrationalism and paralyzing obscurantism.
Religion prevents science from falling into the marsh of
obsolete materialism and moral nihilism.”

Dr. Martin Luther King, Jr.

Table of contents

Chapter 1 General introduction 9

I. Genetic diversity of hepatitis C virus during chronic infection

Chapter 2 Evaluation of persistence of resistant variants with ultra-deep 39
pyrosequencing in chronic hepatitis C patients treated with telaprevir

Chapter 3 Evolutionary dynamics of hepatitis C virus NS3 protease domain during 55
and following treatment with narlaprevir, a potent NS3 protease inhibitor

Chapter 4 Origin and evolution of the unique hepatitis C virus circulating 71
recombinant form 2k/1b

II. Acute hepatitis C virus infections in HIV-infected men who have sex with men

Chapter 5 Alarming incidence of hepatitis C virus reinfection after treatment of 89
sexually acquired acute hepatitis C virus infection in HIV-infected MSM

Chapter 6 Characterization of multiple hepatitis C virus (HCV) infections following 101
acute infection in HIV-infected men who have sex with men (MSM): is
immunity to HCV genotype specific?

Chapter 7 Coalescent analysis of serial hepatitis C virus (HCV) sequences in HIV/HCV 115
coinfection: Is time to the most recent common ancestor a reliable proxy
for infection date?

Chapter 8 Hepatitis C Virus (HCV) antibody dynamics following acute HCV infection 129
and reinfection among HIV-infected men who have sex with men

Chapter 9 Characteristics of Hepatitis C virus (HCV) neutralizing antibody responses 143
during acute HCV infection: are genotypes neutralization serotypes?

III. Summary and general discussion

Chapter 10 Summary 163

Chapter 11 General discussion 169
IV. Appendixes

Nederlandse samenvatting 182

About the author 185

List of publications 186

Portfolio 187

A word of thanks 189