Energy Metabolism and the Regulation of Metabolic Processes in Mitochondria

299 Pages·1972·5.088 MB·English

by  Myron Mehlman (Eds.)

Symposia on Metabolic Regulation
Energy metabolism
and the Regulation of
metabolic Processes
in mitochondria
Edited by
fTlyron fl. IMIman
Department of Biochemistry
University of Nebraska College of Medicine
Omaha, Nebraska
Richard HI. Hanson
Fels Research Institute and Department
of Biochemistry
Temple University Medical School
Philadelphia, Pennsylvania
Academic Press Peuu york and London 1972
COPYRIGHT © 1972, BY ACADEMIC PRESS, INC.
ALL RIGHTS RESERVED
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OTHER MEANS, WITHOUT WRITTEN PERMISSION FROM
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ACADEMIC PRESS, INC.
Ill Fifth Avenue, New York, New York 10003
United Kingdom Edition published by
ACADEMIC PRESS, INC. (LONDON) LTD.
24/28 Oval Road, London NW1
LIBRARY OF CONGRESS CATALOG CARD NUMBER: 70-187235
PRINTED IN THE UNITED STATES OF AMERICA

CONTRIBUTORS
F. J. Ballard, Fels Research Institute and Department of Biochemistry, Temple
University Medical School, Philadelphia, Pennsylvania 19140
Alberto Boveris, Johnson Research Foundation, Department of Biophysics and
Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
Jadwiga Bryla, Johnson Research Foundation, Department of Biophysics and
Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
Britton Chance, Johnson Research Foundation, Department of Biophysics and
Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
Edwin M. Chance, Department of Biochemistry, University College London,
England
David Li-Shan Chao,* Department of Biochemistry, Indiana University School
of Medicine, Indianapolis, Indiana 46202
E. Jack Davis, Department of Biochemistry, Indiana University School of
Medicine, Indianapolis, Indiana 46202
P. Leslie Dutton, Johnson Research Foundation, Department of Biophysics and
Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
Maria Erecinska, Johnson Research Foundation, Department of Biophysics and
Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
/. P. Flatt, Department of Nutrition and Food Science, Massachusetts
Institute of Technology, Cambridge, Massachusetts 02139
*Present address: Department of Biophysical Sciences, University of Houston,
Houston, Texas 77025
xi
CONTRIBUTORS
Alan J. Garber, Fels Research Institute and Department of Biochemistry,
Temple University Medical School, Philadelphia, Pennsylvania 19140
Richard W. Hanson, Fels Research Institute and Department of Biochemistry,
Temple University Medical School, Philadelphia, Pennsylvania 19140
Robert C. Haynes, Jr., Department of Pharmacology, University of Virginia,
Charlottesville, Virginia
L. L. Horstman, Section of Biochemistry and Molecular Biology, Cornell
University, Ithaca, New York 14850
Ferdinand Hucho, * Clayton Foundation, Biochemical Institute and Department
of Chemistry, University of Texas at Austin, Austin, Texas 78712
David Lambeth, Department of Biochemistry and Institute for Enzyme
Research, University of Wisconsin, Madison, Wisconsin 53706
Kathryn F. LaNoue, Johnson Research Foundation, Department of Biophysics
and Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
Henry A. Lardy, Department of Biochemistry and Institute for Enzyme
Research, University of Wisconsin, Madison, Wisconsin 53706
ReneéC. Lin, Department of Biochemistry, Indiana University School of
Medicine, Indianapolis, Indiana 46202
Tracy C. Linn,f Clayton Foundation, Biochemical Institute and Department of
Chemistry, University of Texas at Austin, Austin, Texas 78712
John M. Lowenstein, Graduate Department of Biochemistry, Brandeis
University, Waltham, Massachusetts 02154
CarlR. Mackerer, Department of Toxicology, G. D. Searle & Company,
P. O. Box 5110, Chicago, Illinois 60680
Myron A. Mehlman, Department of Biochemistry, University of Nebraska
College of Medicine, Omaha, Nebraska 68105
Robert M. Oliver, Clayton Foundation, Biochemical Institute and Department
of Chemistry, University of Texas at Austin, Austin, Texas 78712
•Present address: Fachbereich Biologie der Universitat, Kostanz, Germany
f Present address: Department of Biochemistry, University of Texas Southwestern
Medical School, Dallas, Texas 75216
xii
CONTRIBUTORS
/. L. Orr, Department of Biochemistry, University of Toronto, Toronto,
Ontario, Canada
John W. Pelley, Clayton Foundation, Biochemical Institute and Department
of Chemistry, University of Texas at Austin, Austin, Texas 78712
Flora H. Pettit, Clayton Foundation, Biochemical Institute and Department
of Chemistry, University of Texas at Austin, Austin, Texas 78712
Efraim Racker, Section of Biochemistry and Molecular Biology, Cornell
University, Ithaca, New York 14850
Douglas D. Randall* Clayton Foundation, Biochemical Institute and Depart­
ment of Chemistry, University of Texas at Austin, Austin, Texas 78712
Lester J. Reed, Clayton Foundation, Biochemical Institute and Department of
Chemistry, University of Texas at Austin, Austin, Texas 78712
Thomas E. Roche, Clayton Foundation, Biochemical Institute and Department
of Chemistry, University of Texas at Austin, Austin, Texas 78712
Colleen M. Smith, Johnson Research Foundation, Department of Biophysics
and Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
Paul A. Srere, Basic Biochemistry Unit, Veterans Administration Hospital and
University of Texas Southwestern Medical School, Dallas, Texas
75216
R. L. Veech, Section on Neurochemistry, NIMH, IR, AMRDN, and The
National Institute of Alcohol Abuse and Alcoholism, St. Elizabeth's
Hospital, Washington, D. C. 20032
R. W. Von Korff, Maryland Psychiatric Research Center and Friends Medical
Science Research Center, Baltimore, Maryland 21228
Michael Wagner, Johnson Research Foundation, Department of Biophysics and
Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
G. R. Williams, Department of Biochemistry, University of Toronto, Toronto,
Ontario, Canada
•Present address: Department of Agricultural Chemistry, University of
Missouri, Columbia, Missouri 65201
xiii
CONTRIBUTORS
John R. Williamson, Johnson Research Foundation, Department of Biophysics
and Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
David F. Wilson, Johnson Research Foundation, Department of Biophysics and
Physical Biochemistry, University of Pennsylvania, Philadelphia,
Pennsylvania 19104
G. S. Wong, Department of Biochemistry, University of Toronto, Toronto,
Ontario, Canada
xiv
PREFACE
For those of us interested in metabolic regulation, the current methods for
the exchange of information and new ideas seem, at times, inadequate. This
area is so diverse, ranging as it does from the control of mRNA synthesis in
bacteria to the regulation of specific metabolic pathways in man, that it is easy
to understand the difficulty in developing a program for the meaningful
discussion of new research ideas within the general framework of a conventional
biochemical meeting. This problem is compounded by the wide range of
journals that publish articles dealing directly with, or related to, metabolic
regulation. Because of these difficulties we have organized a yearly symposium
on metabolic regulation, which has been held during the past three years at the
University of Nebraska Medical School in Omaha, Nebraska. Each year a
specific topic is covered, and the speakers include scientists already well
known in the area, as well as younger investigators with new ideas and perhaps
a different approach. Wherever possible we present alternative views on a
subject and allow time for free discussion of concepts arising from the formal
presentation. Most importantly, this symposium is open for all to attend. We set
no limit on attendance, and encourage graduate students, postdoctoral fellows,
and scientists to join us in this symposium. Our experience over the past three
years has shown us that we have a good deal to learn about the intricasies of
programming scientific meetings. However, we remain convinced of the wisdom
in our original intention of holding an open forum-type of meeting.
In choosing speakers for this, and for previous symposia, we have relied on
the suggestions of a committee of scientists including Drs. Sidney Weinhouse,
Joseph Katz, Henry Lardy, Howard Katzen, George Cahill, John R. Williamson,
and Ronald Estabrook. Their judgment has been particularly important to us
in the selection of a timely topic and a varied and representative program. We
hope to continue this practice in subsequent symposia but would, of course,
welcome suggestions from interested scientists who might wish to contact us
directly.
The decision to publish this symposium at a time when the number of
journals and books is already overwhelming was not taken lightly. A volume of
this type must fill a need to justify its publication, and only time will tell
whether other scientists working in this area share our enthusiasm for this type
of book. We have drawn together chapters from a number of people actively
xv
PREFACE
engaged in some aspects of metabolic regulation directly concerned with
mitochondrial function. Their contributions vary in style, scope, and method of
presentation, and we have not attempted to dictate in any way the content of
these chapters. Although potential problems, such as a variation in the level of
scientific presentation and a certain unavoidable discontinuity between
chapters, may arise, our aim has been to allow each contributor the broadest
possible discretion. The responsibility for the scientific content of each chapter
lies with the individual authors, although we have tried wherever possible to
clarify and strengthen them by careful and critical review.
We have been greatly aided in our editorial function by Drs. John
Lowenstein, Merton Utter, Henry Lardy, and John Williamson, each of whom
has spent considerable time and effort in reading and commenting on the
manuscripts presented in the session in which they acted as chairmen. Their
help and advice in the preparation of this book have been invaluable, and we are
indebted to them.
We wish to express special appreciation to Dr. and Mrs. Ramon Tate for
their invaluable editorial assistance in helping to prepare these manuscripts for
publication. At the University of Nebraska Medical Center we also had the kind
assistance of Dr. W. R. Ruegamer, Chairman, Department of Biochemistry, the
Department of Continuing Education, and Dr. R. B. Tobin.
We are also particularly indebted to Bill O'Conner and Darlene Patton of
I.B.M. for their generous assistance during the preparation of the manuscripts
for composition.
We would like to acknowledge the fine financial support of the following:
Celanese Chemical Company; Hoffman-La Roche, Inc.; G. D. Searle and Co.;
The Upjohn Company; The Eli Lilly Research Laboratories; Schering
Corporation; Smith, Kline and French Laboratories; Sandoz Pharmaceuticals;
Geigy Pharmaceuticals; Sterling Drug, Inc.; Ciba Pharmaceutical Company; W.
H. Rorer; Merck, Sharp and Dohme; Riker Laboratories, Inc.; Boehringer
Mannheim Corporation; Lakeside Laboratories; Mead Johnson; Charles Pfizer
and Company; The Mogul Corporation; and the Kroc Foundation. The
generosity of these institutions has made the symposium possible.
Myron A. Mehlman
Richard W. Hanson
xvi