Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

264 Pages·2022·7.043 MB·English

by  A. Sankaranarayanan, Natarajan Amaresan, Mitesh Kumar Dwivedi

A. Sankaranarayanan
Natarajan Amaresan
Mitesh Kumar Dwivedi Editors
Endophytic
Microbes: Isolation,
Identifi cation,
and Bioactive
Potentials
S P H
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Endophytic Microbes: Isolation,
Identification, and Bioactive
Potentials
Edited by
A. Sankaranarayanan
Department of Life Sciences, Sri Sathya Sai University for Human Excellence,
Navanihal, Kamalapura, Kalaburagi, Karnataka, India
Natarajan Amaresan and Mitesh Kumar Dwivedi
C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India
Editors
A.Sankaranarayanan NatarajanAmaresan
DepartmentofLifeSciences C.G.BhaktaInstituteofBiotechnology
SriSathyaSaiUniversityforHumanExcellence UkaTarsadiaUniversity
Navanihal,Kamalapura Surat,Gujarat,India
Kalaburagi,Karnataka,India
MiteshKumarDwivedi
C.G.BhaktaInstituteofBiotechnology
UkaTarsadiaUniversity
Surat,Gujarat,India
ISSN1949-2448 ISSN1949-2456 (electronic)
SpringerProtocolsHandbooks
ISBN978-1-0716-2826-3 ISBN978-1-0716-2827-0 (eBook)
https://doi.org/10.1007/978-1-0716-2827-0
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Preface
Theindiscriminateusageoffertilizersandpesticidesnotonlydestroysbeneficialmicrobesin
soil but also acts as a serious threat to the environment. The emerging threats by various
abiotic and biotic factors, including drastic changes in climatic conditions, put agricultural
industrythroughouttheglobeatgreatrisk.Theincreasingtrendsofgreenrevolutionhave
led to the development of promising candidates such as endophytic bacteria and fungi for
sustainable development in agricultural field. Research reports and articles have listed the
roleofendophyticmicrobesinsustainabledevelopmentintheagriculturalfield.
Inthiscontext,wehavemadeanefforttocompileauser-friendlylaboratorymanualon
endophytic microbes. This manual provides the detailed procedures for the isolation and
identification of endophytes, various biochemical methods and molecular methods for the
identification of endophytes, various traditional methods, and modern methods to prove
endophytism,bioactivepotentials,andscreeningoftheirmetabolitesin5differentsections
with29protocols.Thecontentsprovidedinthemanualmaybeusedtocarryoutresearch
workfrombasiclaboratorieswithelementaryfacilitiestohigh-endlaboratories.
We hope that the manual will be highly useful to the research and academic commu-
nities, especially young researchers/students. We are thankful to all contributors to this
book, who spared their valuable time and cooperated with us for bringing this manual
withinthespecifiedtimeperiod.
Healthycriticismandcommentsarehighlywelcomefromthereaders.
Kalaburagi,Karnataka,India A.Sankaranarayanan
Surat,Gujarat,India NatarajanAmaresan
Surat,Gujarat,India MiteshKumarDwivedi
v
Contents
Preface ..................................................................... v
Contributors................................................................. xi
PART I CULTIVATION METHODS OF BACTERIAL ENDOPHYTES
1 IsolationofEndophyticBacteriafromLeaves,Stems,orRoots....... ........ 3
JoycePriscaBakuwaNjoloma
2 Heat-TreatmentMethodforIsolationofBacillusspp.
fromPlantTissues ......... ........ ....... ....... ........ ....... ........ 7
T.R.Kavitha,C.Suneetha,andT.R.Sunitha
3 IsolationandDetectionofEndophyticStreptomycesfromActinorhizal
CasuarinaRootNodules ........... ....... ....... ........ ....... ........ 15
ThirumagalThirugnanamandDhanasekaranDharumadurai
4 IsolationofEndophyticFungifromStems,Leaves,orRoots...... ... ........ 27
V.Ambikapathy,N.Sengottaian,A.S.ShijilaRani,
S.Babu,andA.Anbukumaran
PART II BIOCHEMICAL METHODS FOR IDENTIFICATION OF ENDOPHYTES
5 DetectionofEndophytesbyCultivationMethod .... ........ ....... ........ 33
PaulaRodrı´guez,DavidGonzalez,andSoniaRodrı´guezGiordano
6 DetectionofEndophytesbyVisualizationMethods.......... ....... ........ 43
VijayalakshmiSelvakumar,KarnanMuthusamy,
ThenmozhiManivel,PreethiGopalsamy,PanneerselvamAnnamalai,
RamachandranChelliah,andDeog-HwanOh
7 DetectionofEndophytesbyImmunologicalMethods........ ....... ........ 51
BankapuraMariyappaRavikumara,LakshmaiahManjunatha,
KittanSubathra,GummeralNarasareddy,GangadharaiahJyothi,
andChikkabuddiPrashantha
8 DetectionofEndophytesbyElectronMicroscope ........... ...... ....... .. 71
PradeepKumar,SimranRani,Sarita,AmitaSunejaDang,
andPoojaSuneja
9 DetectionofEndophytesbyReactiveOxygenStaining ....... ....... ........ 77
SimranRani,PradeepKumar,Deepika,AmitaSunejaDang,
andPoojaSuneja
PART III MOLECULAR METHODS FOR IDENTIFICATION OF ENDOPHYTES
10 IdentificationofBacterialEndophytesby16SrRNA ......... ....... ........ 85
V.Ambikapathy,S.Babu,R.Shanmugapriya,
A.Prakash,andA.S.ShijilaRani
vii
viii Contents
11 IdentificationofFungalEndophytesbyITSrDNATechnique........ ........ 89
PoojaSuneja,PradeepKumar,SimranRani,
Simran,andAmitaSunejaDang
12 IdentificationofFungalEndophytesby28SrDNATechnique........ ........ 97
KarnanMuthusamy,TamilkaniPichai,VijayalakshmiSelvakumar,
AnbukumaranAnanthan,ReshmaDeviRamesh,
LeelavathiLakshmanan,SenthilkumarGovindarajan,
PanneerselvamAnnamalai,andKiChoonChoi
13 DetectionofEndophytesbyMolecularFingerprintingTechniques.... ........ 109
LakshmaiahManjunatha,GummeralNarasareddy,
BankapuraMariyappaRavikumar,LeelaSaisreeUppala,
RishikeshKumar,NanjundappaManjunatha,
ManikyanahalliChandrashekaraKeerthi,
RajKumarMishra,andBansaSingh
14 MetagenomicsandPyrosequencingApproach....... ........ ....... ........ 135
JeanCarlosVencioneckDutra
andMariadoCarmoPimentelBatitucci
PART IV METHODS TO PROOF ENDOPHYTISM
15 Cultivation-BasedStudiesofEndophyticFungiinPlants ..... ....... ........ 145
LizzyA.Mwamburi
16 Microscopy-BasedStudies .......... ....... ...... ....... ......... ........ 153
C.Brı´gidoandA.Pac¸o
17 SurrogateHostTest........ ........ ....... ....... ........ ....... ........ 169
HarshidaA.GamitandNatarajanAmaresan
18 HoodandShewStaining ........... ....... ....... ........ .. ..... ...... .. 173
HarshidaA.GamitandNatarajanAmaresan
PART V ENDOPHYTES FOR BIOACTIVE METABOLITES
19 ScreeningofEndophytesforPlantGrowth-PromotingMetabolites........... 179
RinkaS.Pramanik,SapnaChandwani,andNatarajanAmaresan
20 ScreeningofEndophytesforBiocontrolProperties .......... ....... ........ 189
ShahRushabh,SapnaChandwani,andNatarajanAmaresan
21 ScreeningofEndophytesforBiocatalyticTools...... ........ ....... ........ 193
DavidGonzalez,EmilianaBotto,SoniaRodrı´guezGiordano,
andPaulaRodrı´guezBonnecarrere
22 ScreeningofEndophytesforBiosurfactantProduction....... ....... ........ 209
V.Ambikapathy,S.Babu,A.Anbukumaran,andA.S.ShijilaRani
23 ScreeningofEndophytesforAntibacterialProperties........ .... .... ........ 213
M.Veerapagu,K.R.Jeya,andA.Sankaranarayanan
24 ScreeningofEndophytesforNematicidalActivities .......... ....... ........ 225
SomaBarman,RatanChowdhury,andSatyaSundarBhattacharya
Contents ix
25 ScreeningofEndophytesforVirucidalActivity ...... ........ ....... ........ 237
L.Manjunatha,G.NarasaReddy,LeelaSaisreeUppala,
B.M.Ravikumara,andR.Ranjini
26 ScreeningofEndophytesforFungicidalActivities.... ........ ....... ........ 245
K.R.Jeya,M.Veerapagu,andA.Sankaranarayanan
27 ScreeningofEndophytesforAnticancerCompounds ........ ....... ........ 255
DriellyDayanneMonteirodosSantosBaliza,
JulianaFonsecaMoreiradaSilva,Eska´lathMorgannaSilvaFerreira,
IrlonM.Ferreira,EdileneOliveiradaSilva,
Jose´LuizMartinsdoNascimento,andRaphaelSanzioPimenta
Index ...................................................................... 269
Contributors
NATARAJANAMARESAN • C.G.BhaktaInstituteofBiotechnology,UkaTarsadiaUniversity,
Surat,India
V.AMBIKAPATHY • DepartmentofBotany,A.V.V.M.SriPushpamCollege(Autonomous),
(AffiliatedtoBharathidasanUniversity,Trichy),Thanjavur,TamilNadu,India
ANBUKUMARAN ANANTHAN • DepartmentofMicrobiology,UrumuDhanalakshmiCollege,
Trichy,Tamilnadu,India
A.ANBUKUMARAN • DepartmentofMicrobiology,UrumuDhanalakshmiCollege,(Affiliated
toBharathidasanUniversity),Trichy,TamilNadu,India
PANNEERSELVAMANNAMALAI • PGandResearchDepartmentofMicrobiology,A.V.V.M.Sri
PushpamCollege(Autonomous),Thanjavur,Tamilnadu,India;PG&Research
DepartmentofMicrobiology,AVVMSriPushpamCollege(Auto),Thanjavur,Tamilnadu,
India
S.BABU • DepartmentofBotany,A.V.V.M.SriPushpamCollege(Autonomous),(Affiliated
toBharathidasanUniversity,Trichy),Thanjavur,TamilNadu,India
SOMA BARMAN • SoilandAgro-BioengineeringLaboratory,DepartmentofEnvironmental
Science,TezpurUniversity,AssamTezpur,India
SATYASUNDARBHATTACHARYA • SoilandAgro-BioengineeringLaboratory,Departmentof
EnvironmentalScience,TezpurUniversity,Tezpur,Assam,India
EMILIANABOTTO • LaboratoriodeBiocata´lisisyBiotransformaciones,DEPBIO-DQO,
UniversidaddelaRepu´blica(UdelaR),Montevideo,Uruguay
C.BRI´GIDO • MED-MediterraneanInstituteforAgriculture,Environmentand
Development&CHANGE-GlobalChangeandSustainabilityInstitute,Institutefor
AdvancedStudiesandResearch,UniversidadedeE´vora,E´vora,Portugal
SAPNACHANDWANI • C.G.BhaktaInstituteofBiotechnology,UkaTarsadiaUniversity,Surat,
India
RAMACHANDRAN CHELLIAH • FoodScienceandBiotechnology,SchoolofAgricultureandLife
Sciences,KangwonNationalUniversity,Chuncheon,RepublicofKorea
KICHOONCHOI • GrasslandandForageDivision,NationalInstituteofAnimal
Science,Seoul,SouthKorea
RATANCHOWDHURY • DepartmentofBotany,RangaparaCollege,Amaribari,Rangapara,
Assam,India
AMITASUNEJADANG • CentreforMedicalBiotechnology,M.D.University,Rohtak,
Haryana,India
EDILENEOLIVEIRADASILVA • InstitutodeCieˆnciasBiol(cid:1)ogicas,UniversidadeFederaldoPara´,
Bele´m,PA,Brazil
JULIANAFONSECAMOREIRADASILVA • UniversidadeFederaldoTocantins,Palmas,TO,
Brazil
DEEPIKA • DepartmentofMicrobiology,M.D.University,Rohtak,Haryana,India
DHANASEKARANDHARUMADURAI • DepartmentofMicrobiology,SchoolofLifeSciences,
BharathidasanUniversity,Tiruchirappalli,TamilNadu,India
MARIADO CARMOPIMENTELBATITUCCI • Laborat(cid:1)oriodeGene´ticaVegetaleToxicol(cid:1)ogica,
DepartamentodeCieˆnciasBiol(cid:1)ogicas,UniversidadeFederaldoEspı´ritoSanto,Vit(cid:1)oria,
Espı´ritoSanto,Brazil
xi