Effect of various heavy metals on the enzymatic activity of bacterial alkaline phosphatase

125 Pages·2016·13.86 MB·English

United Arab Emirates University
Scholarworks@UAEU
Theses Electronic Theses and Dissertations
2010
Effect of various heavy metals on the enzymatic
activity of bacterial alkaline phosphatase
Maithah Maktoom Salmeen Al Nuaimi
Follow this and additional works at:https://scholarworks.uaeu.ac.ae/all_theses
Part of theEnvironmental Sciences Commons
Recommended Citation
Salmeen Al Nuaimi, Maithah Maktoom, "Effect of various heavy metals on the enzymatic activity of bacterial alkaline phosphatase"
(2010).Theses. 371.
https://scholarworks.uaeu.ac.ae/all_theses/371
This Thesis is brought to you for free and open access by the Electronic Theses and Dissertations at Scholarworks@UAEU. It has been accepted for
inclusion in Theses by an authorized administrator of Scholarworks@UAEU. For more information, please [email protected].

Unietd rabE miarteU nievsrit)
Dean ohfGi rapdu atSet udie
M. c.P rogarmi nE nvoinmrentSacli ee nc
Effeco"tf aioruhse amve)t aoln t heen yzmataicct iitovyfb actearlaiklailn e
phop hatea
By
MaithMaahk tooSmal meenA IN uaimi
A the is
Subimtted to
UneidtA rabE miarteU nvieir't )
Inp atrifaullifm lenotft hree qieurmnets
Fotrh dee gereo fM .S ci.nE nvoinmrenatlS ciee nc
uprevoir
Dr. a.l maA nh ar,f
A ociaPtreoe f oro fB oichiet mry,
DepartomfCe hnetm tiry ,
Facl)uto' fS ciceen,
UAEU nvieir)t
2009-2 010

'"' .'
UnietdA rabE miaret Univseiyrt
Deahni opfG raduaSttued ies
M.S cP.ro garmi nE mi rmoenn tcaile en c
Effceto fv arihoeauv mye taolnst heen ZJmaatcitcii ytov fb acrtieaallak line
phsophaat e
By
MaithMaahk tooSmam leeAnI N uaimi
A thseis
Submittetdo
UnietdA rabE miarteU niveir't )
Inp artfiuliafllm eonft thr ee qiuerment
Fotrh deeg reoefM .S ci.nE nvoinmrentSacile en c
Supen'oir
Dr..S almaAn h ar,f
A soceiP arotef oorfB iochterym,i
DeptamreonftC hemtiry ,
Facluto)fS ciceen,
UAE Univeirt y
2009-2 010
UAEU IBRARIES
ItIIIIII IIIIII IUIU11I1 11111 1
1000474849
TheT hesio fM aithaM aktoomA l Nuaimif ort heD egreoef M asteorf
ciencienE nvironnmt ails a pproved.
Examininogm mitteMee mberD,r .S yedS almanA sh
Iv
y�
...........................�. ............................... .......................... .
ExaminiCnogm mitteMee mberD,r .Y usraO thman
ExaminiCnogm mitteMee mberD,r .A hmed M. Almehdi
�
'1 '. n
t
PrograDmi rectDorr.,T arekY oussef
ActinDge anC,o llegofeS ciencPer,o fM.o hammed N.A nwar
UniteAdr abE miratUensi versity
200912010
Dedication
fetihe¥ �Mothe"y, £UnlPy� t&1Je;yOU¥'
io-t1'o/ I
�!
11
Acknowledgeemnt
Ia md eplidyne btteomd y upveiro rD r.am lanA srha,Af ssioacPtreeo sfsor
ofB icoehmtir w)h\og ae\m et hheo notuobr eo neo fh isst udeAnlotsf sorh. i s
,laaubladev iacnhdee ltrpho ughmoywu htlo teh e-sfriosmt ehe xperipmaetrnott al
threei vewionftg h tihse Isw iousl.ad lo ls kiet o tmhyat nhke esxiaisnm erDsrY .us ra
Othmnaa nd ADhmre.dA lemhd-bio tohfw hosmp eanl too tft iema nedff roit n
rveei\'i,n agn edx maiinnmgy t hetsofiur sht eirm priotvte.ur layp epcraitaellt heir
r
harwdo rakn hde ,le pspiealclyDr A.lm ehdie'yrt soh orughhe !l p
AdidotnilaylIw, a nttot haanltklh f eca ulmteymb erasnt dh set fam fembeorfs
thEen voinrmenStcailep nrcoeg arnatmdh Dee ptamreonfCt h emistfroryt hehierl p
ansdu ppSopreitca.tl h anarkaeslo ds uteoI t besAabmd Sua efeordh eplinmgew ith
parotfts h eexp emreintwaolra ksw elalsw itdha taanl aysTihsa.yn oku msuco!h
Fniall,y wIou lldie t koe xpremsysg riatdtuet oal lt ohsweh og avet hmee
possiibtityloc omptlee thsiiEsss .pct eihaemlyld ye,ah rub sanMdoh amemdf,ma ily
memberasnm dy f irendAsis hMa,ar aimA,fr aA,b eeSrha,m ma,A zzaan,Nd o ura
whok epetn caoguirnmgef rmo thvee rbye ngniiwnhge Ine norlldei n ptrhoeg .r am
111
Abstract
oiila n aturreoa urlc teht a uitsm toi mportfaornmca ena gcrultiuarals w elals
envoinrmenptearcl t pi Ovoeo.do ihlel atnhoo tn lhyes li pmprotvheqe u iatlayn d
yieolfad g cruiulrtper oudctbsui,tas l 0 i moprtfaronr tce yicgnlo fs ingiiafcnt
amounotfo rgiabcni osmsa. eHvoepwrol.lt iunoo fs ioblyv aoruipsol ultanstusca,hs
he'mYm et.ap leisictdesp eotlrrue omh yodcrarbcoanlnase d,t os eriou
envoinrmenatswa ellal sb ioglioceaffcelt .T hipsol ltiucoandn ie crthlayr amn imals
anhdu mabnei gnsl,e aictnhod rnikgiw natseoru rdcaem,ap glneat asn vde geitoaants,
welalsa ffectth e eemnimdci ocorargnmiss ainnedsc ltvsiig ni tnh seol i.
Miocorrgimassnt ahta rneo mralflouyn idnt h teo spol pi lyak erylo sei nen yzmatic
rceycloifnnt igr ogpehno,so pruhosa,n sdu ulrf,w ella st hdee coomspitoifo n
as
oragnimact :tt ehruthse iymp acgtl orbceacylil nogfn utrtisec,na rbaonnd eort h
elmeentIsan.d i dtitoonn ut rireeynccltig ,ns iomlic robceaasnl o as ffecpth ysical
propieeorsfst olai st hes ecreexatt-eerc llluaprol syacchawrhiidscetbhsa ii elzs iol
aggregwahtieicsnth,u mf eacwfta tretere ntiiniotflnri,ao trna tcer,ui sn,etg rdoiibtiyl
ofs iolTh.u msic,r ioabhlae litcsho ndseiraeg do oiddn itcoaorfs olhi aeltHhe.ay
metpaollul tafnoutnsid nt h seio clo cna eut shedierle ertieoffuescb tyso noeff ou r
ways:T heeh seamveyt caalunsn dgeorr edcocyxln gia nidnt hper ocleaesds to
I)
oxaydripcraouldc twihointc hhec nas ueosx aiitdevs treosrsg iaisnnm2 s)t,eh yc an
bidnd iecrttlosy u blytdyrlg rouopfps r oitsnae nldae dt op rtoeiiannc ttiivaoannd
denuaartito3n)t, h ecyab ni dnt oi nrtacelalgrlul utaotnh(eiOS Ho)ra ntiidoaxnt
enzy(mees.O gD.,C ataalsGeS,H r edtuac/sp eeorxidaeesa)n rdde ucteh e
antiaonxtil tdiayob fci el;lsa n4d) t heeh seamveyt sac laanlo cs omeptfero m eatl­
coafctboidrni nogfm eatl-leonzyamneldase d t o tihanecitairito Rne.c ewnotro kn
JV