Effect of Microwave Cured Acrylic Resin on Candidal Growth in Complete denture.

118 Pages·2009·1.51 MB·English

Effect of Microwave Cured Acrylic
Resin on Candidal Growth in
Complete denture.

Thesis
Submitted to the Faculty of Dentistry, Ain Shams
University in partial fulfillment of the Requirements for
the Master’s Degree in Prosthodontics

By
Amr Hassan Mousa Rasmy
B.D.S (2002) Ain Shams University

Faculty of Dentistry
Ain Shams University
2009
SUPERVISORS

Prof. Dr. Ingy Talaat Lebshtien
Professor and Chairman of Prosthodontic Department
Faculty of Dentistry
Ain Shams University

Dr.Suzan Abd El Halim Abdallah.
Associate Prof of Microbiology (Plant Pathology).
Faculty of Science, Botany Department, Banha
University

Dr.Rami Maher Ghali.
Lecturer of Prosthodontics Department
Faculty of Dentistry
Ain Shams University

Ain Shams University
2009
Acknowledgment
Allah
First of all, thanks to who granted me the
ability to accomplish this work.
I would like to express my most sincere gratitude
and grateful appreciation to Prof. Dr. Ingy Talaat
Lebshtien, professor and chairman of prosthodontic department,
faculty of Dentistry, Ain shams university , in spite of all her great
responsibilities , she always find the time to support, guide and
encourage me. It was a great honor to work under her wise
sympathetic, encouraging supervision and I would like to declare
how much I learnt from her.
Countless thanks are extended to Dr.Suzan Abd El
Halim Abdallah, Associate professor of Microbiology
(Plant Pathology), faculty of science, Botany Department,
Banha university, for her Supervision, support , generosity
in giving advice, and guidance during the course of this
research.
I would like to present my deepest thanks and
appreciation to Dr.Rami Maher Ghali, Lecturer of
prosthodontic, prosthodontic department, faculty of
dentistry, Ain shams university, for his valuable support,
patietnce,advise and assistance. I would like to declare how
much I learnt from him.
Last but not least I would like to thanks members
of my family for their unlimited support, understanding
and encouragement especially my mother and my brother.
Finally , I would like to dedicate this work to the
soul of My dearest Father.
CONTENTS

Title Page no
List of tables I
List of figures II,III,IV
Introduction 1
Review of Literature 4
I.1.Denture base material: 4
I.2.Bio-compatibility of denture base
14
resins:
I.3.Adverse reactions to denture base
15
resins:
I.4.The Resident oral micro flora:- 19
I.5.Candida albicans:- 21
I.6.Denture stomatitis: 26
I.7.Pathogenicity of Candida albicans
28
in denture stomatitis:
I.8.Intra-oral adhesion of Candida
29
albicans:
I.9.Laboratory diagnosis of denture
33
stomatitis:
Aim of the study 38
Materials and methods 39
II.1.Patients Selection: 39
II.2.Patient’s examination: 40
II.3.Grouping of Patients 42
II.4.Denture Construction: 42
II.5.Patient instructions: 48
II.6.Rebasing: 49
II.7.Collecting the Samples: 55
II.8. Light microscopic examination 57
II.9.Morphological investigation by
59
scanning electron microscope:
Results 61
III.1.The effect of conventional
heat-cured acrylic denture resin 61
on Candidal growth:
III.2.The effect of microwave-cured
acrylic denture resin on 63
Candidal growth:
III.3.Comparison between
conventional heat-cured and
microwave-cured acrylic 65
denture resin on Candidal
growth:
Discussion 76
Summary and Conclusions 87
References 90
Arabic summary 106
List of Tables

Page Title Table
no
The prevalence of colony forming unit
(CFU) and fisher’s exact test in heat
1 62
cured acrylic denture base during the
follow up cases
The prevalence of colony forming unit
(CFU) and fisher’s exact test in
2 64
microwave -cured acrylic denture base
during the follow up cases
Comparison between the effect of
conventional heat-cured and
3 microwave-cured acrylic denture on 66
candidal growth before denture
insertion
Comparison between the effect of
conventional heat-cured and
4 microwave-cured acrylic denture on 74
Candidal growth after one month
interval
List of Figures 
Figure Title Page
no.
1 A,B and C; A, Primary upper alginate
impression; B, Primary lower 44
alginate impression and C, Primary
upper and lower alginate impression.
2 A,B and C; A, Secondary upper
rubber base impression; B,
45 
Secondary lower rubber base
impression and C, Secondary upper
and lower rubber base impression
3 Try-in stage in denture construction 47 
4 Finished and delivered conventional
48 
heat-cured upper and lower complete
denture
5 A, B, C and D; A, upper complete
Denture mounted on upper member
52 
of the articulator, B, C and D
occlusal index being established on
lower member of the articulator
6 Acrylic resin teeth cut from denture
53 
base using a bur
7 Acrylic resin teeth after being cut
from denture base was seated in their
53 
indentations in occlusal index on
lower member of the articulator
8 A and B, microwave flask; A,
microwave flask assembly and B,
microwave flask parts
54
Figure Title Page
no.
9 A, B and C; A, representative
samples of oral rinse of two patients
using the oral rinse technique; B,
Negative growth of Candida albicans
culture from sample four shown in
fig. 9,A (arrowed) grown on
56 
sabouraud’s dextrose agar medium
for 48H at 37°C; and C, positive
growth of Candida albicans culture
from sample three shown in fig. 9,A
(arrowed) grown on sabouraud’s
dextrose agar medium for 48H at
37°C .
10 Light microscopy of Candida
albicans grown on sabouraud’s
57
dextrose agar medium stained by
gram stain (+ve) showing budding
stage (arrowed).
11 Light microscopy of Candida
albicans grown on sabouraud’s
dextrose agar medium stained by
58 
gram stain(+ve) showing various
fungal structures [hyphae (arrowed)
and germ tube (GT)].
12 Light microscopy of Candida
albicans grown on human serum for
2-3 h. 37°C showing Condia(C),
59 
Germ tube (GT) and aggregates of
spores in clusters (arrowed).

13 The prevalence of colony forming
unit (CFU) scores within group (I)
63 
before and after one month of the
denture insertion.
Figure Title Page
no.
14 The prevalence of colony forming
unit (CFU) scores within group (II)
65 
before and after one month of the
denture insertion.
15 The prevalence of Colony forming
units (CFU) in conventional heat-
67 
cured and microwave-cured acrylic
denture base resin before denture
insertion.
16 The prevalence of Colony forming
units (CFU) in conventional heat-
75 
cured and microwave-cured acrylic
denture base resin one month after
denture insertion
17 A, B, C, D and E, Scanning electron
microscopy photography of Candida
albicans sampled from patient no. 5
one month after wearing
conventional heat-cured complete 69-70
denture; A, general view of Candida

albicans; B, enlarged portion of
insert shown in Fig. (17, A); C,
mono-polar budding (arrowed); D,
bi-polar budding (arrowed) and E, tri-
polar budding (arrowed).
A and B, scanning electron
microscopy photography of Candida

albicans samples from patient no. 5
after one month wearing microwave-
18  71
cured complete denture; A, showing

budding stage of different forms
(arrowed) and germ tube (GT); B,
mucilaginous material surrounding
the Pseudohyphae (arrowed).
Figure Title Page
no.
19 A and B, Scanning electron
microscopy photography sampled
from patient no. 7 after one month
of wearing conventional heat-cured
complete denture showing
72
developmental stages of Candida
albicans; A ungerminated Conidia
(C) and budding of Candida
albicans (arrowed); B, Pseudohyphae
(arrowed).
20 Scanning electron microscopy
photography sampled from patient
no. 7 after one month wearing 73
microwave-cured complete denture
showing different budding stages
(arrows).