DTIC ADA546698: Acute Dermal Irritation Study of JP-8 and S-8 in New Zealand White Rabbits

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by  Defense Technical Information Center

AFRL-RH-WP-TR-2011-0054

Acute Dermal Irritation Study of JP-8 and S-8 in
New Zealand White Rabbits

Jonathan M. Hurley
WIL Research Laboratories, LLC
1407 George Road
Ashland, OH 44805-8946

LT Dean Wagner
Naval Health Research Center
Environmental Health Effects Lab
2729 R St. Bldg. 837 Area B
Wright Patterson AFB, OH 45433-5707

Teresa R. Sterner
Henry M. Jackson Foundation for the
Advancement of Military Medicine
Wright-Patterson AFB, OH

David R. Mattie
Biosciences and Performance Division
Applied Biotechnology Branch

January 2011

Interim Report for June 2008 to September 2008

Air Force Research Laboratory
Distribution A: Approved
711th Human Performance Wing
for public release;
distribution unlimited. Human Effectiveness Directorate
Biosciences and Performance Division
Applied Biotechnology Branch
WPAFB, OH 45433-5707

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1. REPORT DATE (DD-MM-YYYY) 2. REPORT TYPE 3. DATES COVERED (From - To)
31-01-2011 Interim 4 Jun 2008 – 26 Sep 2008
4 . TITLE AND SUBTITLE 5a. CONTRACT NUMBER
Acute Dermal Irritation Study of JP-8 and S-8 in New Zealand White
Rabbits
5b. GRANT NUMBER
NA
5c. PROGRAM ELEMENT NUMBER
62202F
6. AUTHOR(S) 5d. PROJECT NUMBER
Hurley, Jonathon M.**, Wagner, Dean***, Sterner, Teresa R.****, Mattie, OAFW
David R.*
5e. TASK NUMBER
P0
5f. WORK UNIT NUMBER
OAFWP002
7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION
WIL Research Laboratories, LLC** Naval Health Research Center*** REPORT NUMBER
1407 George Road Environmental Health Effects Lab
Ashland, OH 44805-8946 2729 R St. Bldg. 837 Area B
Wright Patterson AFB, OH 45433-5707
HJF****, 2729 R Street, Bldg 837
Wright-Patterson AFB, OH 45433-5707
9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S)
Air Force Materiel Command* 711 HPW/RHPB
Air Force Research Laboratory
Human Effectiveness Directorate 11. SPONSORING/MONITORING
AGENCY REPORT NUMBER
Biosciences and Protection Division

Applied Biotechnology Branch
AFRL-RH-WP-TR-2011-0054
Wright Patterson AFB OH 45433-5707
12. DISTRIBUTION AVAILABILITY STATEMENT
Distribution A: Approved for public release; distribution unlimited.
13. SUPPLEMENTARY NOTES
Cleared 18 Jul 2011; 88ABW-2011-3905
14. ABSTRACT
The objective of this study was to determine the irritative potential of JP-8, S-8 and a 50/50 volume/volume mixture of
JP-8 and S-8 following a single exposure to the skin of albino rabbits. Three groups of three albino rabbits received a
single, four-hour, occluded exposure and an additional three groups received a single, four-hour, semi-occluded
exposure. Doses (0.5 mL) of the test substances were applied to clipped, unabraded skin. At the completion of all
exposures, the bandages were removed and the sites washed. Application sites were evaluated at approximately 30 to
60 minutes; 24, 48 and 72 hours; and 4, 7 and 14 days after patch removal for sites where irritation persisted. Occluded
exposures of JP-8 or S-8 resulted in a classification of moderately irritating. Semi-occlusion of either JP-8 or S-8
produced slight irritation. The mixture, however, was slightly irritating in both the occluded and semi-occluded
exposures.
15. SUBJECT TERMS
JP-8, S-8, jet fuel, synthetic fuel, dermal, skin, mixture
16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF 18. NUMBER 19a. NAME OF RESPONSIBLE PERSON
ABSTRACT OF PAGES David R. Mattie
a. REPORT b. ABSTRACT c. THIS PAGE 69
U U U 19b. TELEPONE NUMBER (Include area code)
SAR NA
Standard Form 298 (Rev. 8-98)
Prescribed by ANSI-Std Z39-18
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TABLE OF CONTENTS

List of Figures ................................................................................................................................ iv
List of Tables ...................................................................................................................................v
Preface........................................................................................................................................... vii

1.0 Summary ...................................................................................................................................1
2.0 Introduction ...............................................................................................................................2
3.0 Materials and Methods ..............................................................................................................3
3.1 Test Substances .............................................................................................................3
3.2 Test Animals .................................................................................................................3
3.3 Test Substance Administration .....................................................................................4
3.4 Observations .................................................................................................................5
3.5 Data Retention ..............................................................................................................6
4.0 Results and Discussion .............................................................................................................6
5.0 Conclusions .............................................................................................................................11
6.0 References ...............................................................................................................................11

Appendix A. Laboratory Test Reports ..........................................................................................12
Appendix B. Animal Room Environmental Conditions ...............................................................17
Appendix C. Study Protocol .........................................................................................................23

List of Abbreviations .....................................................................................................................38

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LIST OF FIGURES

Figure 1. General locations of application sites ..............................................................................5

iv
LIST OF TABLES

Table 1. Test substances identification ...........................................................................................3
Table 2. Study group assignments ..................................................................................................4
Table 3. Evaluation of dermal reactions .........................................................................................5
Table 4. Descriptive ratings: Mean Primary Dermal Irritation Index .............................................6
Table 5. Individual dermal scores for the JP-8 occluded test group ...............................................7
Table 6. Individual dermal scores for the JP-8 semi-occluded test group ......................................7
Table 7. Individual dermal scores for the S-8 occluded test group ................................................8
Table 8. Individual dermal scores for the S-8 semi-occluded test group ........................................8
Table 9. Individual dermal scores for the JP-8/S-8 occluded test group ........................................9
Table 10. Individual dermal scores for the JP-8/S-8 semi-occluded test group .............................9
Table 11. Individual body weights................................................................................................10

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PREFACE

Funding for this project was provided through the Air Force Research Laboratory, Propulsion
Directorate, Fuels Branch (Dr Tim Edwards, AFRL/RZPF) and the Alternative Fuels
Certification Office (AFMC 77 AESW/LF). This research was conducted under contract
FA8601-07-P-0241. The program manager for the contract was LT Dean Wagner, PhD, USN
Naval Health Research Center/Environmental Health Effects Laboratory (NHRC/EHEL). The
technical manager for the program under which this project was conducted, Fischer Tropsch (F-
T) Jet Fuel Toxicity Assessment, was Dr David Mattie. The authors acknowledge the following
individuals who also served on a review panel for this program and this project: John Hinz
(USAFSAM/OEHTH, Brooks City Base, TX); Gunda Reddy, PhD (USACHPPM, Aberdeen
Proving Ground, MD); David Steup, PhD (Shell Oil Company, Houston, TX; Chairman,
American Petroleum Institute-Toxicology Task Force); and Errol Zeiger, Ph.D., J.D. (Errol
Zeiger Consulting, Chapel Hill, NC).

This study, designated WIL-647001, was conducted in compliance with the United States
Environmental Protection Agency (U.S. EPA) Good Laboratory Practice Standards (40 CFR Part
792, 1989); the standard operating procedures of WIL Research Laboratories, LLC; and the
protocol as approved by the sponsor. Analytical confirmation of the concentration, homogeneity
and stability of the dosing mixture was not supplied by the sponsor and was not conducted as
part of this study.

The study protocol was designed to be in general compliance with the U.S. EPA Office of
Prevention, Pesticides and Toxic Substances (OPPTS) Guideline 870.2500 (U.S. EPA, 1998), the
Organisation for Economic Cooperation and Development (OECD) Guidelines for Testing of
Chemicals, Section 404 (OECD, 2002).

This animal study was approved by the Air Force Surgeon General’s Research Human & Animal
Research Panel and the WIL Research Animal Care and Use Committee. The study was
conducted in a facility accredited by the Association for the Assessment and Accreditation of
Laboratory Animal Care, International, in accordance with the Guide for the Care and Use of
Laboratory Animals (NRC, 1996).

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