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144 Pages·2008·2.47 MB·English

Tendon Cell Behavior
and Matrix Remodeling in
Degenerative Tendinopathy
Marieke de Mos
Printing of this thesis was financially supported by Stichting Anna Fonds, Nederlandse Vereni-
ging voor Matrix Biologie, Biomet, Reumafonds.
ISBN: 978-90-8559-453-6
Cover by: Marieke de Mos
Layout and printing: Optima Grafische Communicatie, Rotterdam, The Netherlands
© M. de Mos, The Netherlands, 2008. All rights reserved. No part of this thesis may be reproduced
or transmitted in any form or by any means, without prior written permission by the author.
Tendon Cell Behavior
and Matrix Remodeling in
Degenerative Tendinopathy
Peescelgedrag en matrix remodellering in
degeneratieve tendinopathie
Proefschrift
ter verkrijging van de graad van doctor aan de
Erasmus Universiteit Rotterdam
op gezag van de
rector magnificus
Prof.dr. S.W.J. Lamberts
en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op
woensdag 7 januari 2009 om 11.45 uur
door
Marieke de Mos
geboren te Voorburg
PRoMoTie CoMMissie
Promotor: Prof.dr. J.A.N. Verhaar
Overige leden: Prof.dr. J.M.W. Hazes
Prof.dr. S.E.R. Hovius
Prof.dr. P.R. Van Weeren
Copromotor: Dr. G.J.V.M. van Osch
Voor mama

Contents
List of Abbreviations 9
Chapter 1 General introduction 11
Chapter 2 Achilles tendinosis: changes in biochemical composition and 25
collagen turnover rate
Chapter 3 Tendon degeneration is not mediated by regulation by Toll-like 43
receptors 2 and 4 in human tenocytes
Chapter 4 Intrinsic differentiation potential of adolescent human tendon 55
tissue: an in-vitro cell differentiation study
Chapter 5 In-vitro model to study chondrogenic differentiation in 73
tendinopathy
Chapter 6 Can platelet-rich plasma enhance tendon repair: a cell culture study 89
Chapter 7 General discussion 105
Chapter 8 Summary 117
Appendices 123
Nederlandse samenvatting 125
Dankwoord 129
Curriculum Vitae 133
PhD Portfolio Summary 135
List of Publications 137
Color figures 139

List of Abbreviations
α-SMA alpha smooth muscle actin
AA Achilles tendon tissue adjacent to tendinotic lesion
AGC1 aggrecan
AGE advanced glycation endproduct
AH healthy Achilles tendon tissue
AT tendinotic Achilles tendon tissue
BGLAP bone gamma-carboxyglutamate = osteocalcin
BMSC bone marrow-derived stromal cell
CBFA1 core-binding factor 1 = RUNX2
COL10A1 collagen type X, alpha 1 chain
COL2A1 collagen type II, alpha 1 chain
D7-FIB 11-fibrau, fibroblast antibody
DMEM Dulbecco’s modified Eagle’s medium
ECM extracellular matrix
EGF epidermal growth factor
FABP adipocyte fatty acid binding protein
FACS fluorescense activated cell sorter
FCS fetal calf serum
FGF fibroblast growth factor
GAG glycosaminoglycan
HGF hepatocyte growth factor
HP hydroxylysylpyridinoline
HPLC high-performance liquid chromatography
Hyl hydroxylysine
Hyp hydroxyproline
IGF insulin-like growth factor
IHC immunohistochemistry
IL interleukin
LP lysylpyridinoline
MEC medical ethical committee
MMP matrix-metalloproteinase
OA osteoarthritis
PDGF platelet-derived growth factor
PPARG peroxisome proliferative activated receptor gamma
PPCR platelet-poor clot releasate
PPP platelet-poor plasma
PRCR platelet-rich clot releasate
PRP platelet-rich plasma
QPCR quantitative polymerase chain reaction
RA rheumatoid arthritis
RFU relative fluorescence unit
RT-PCR reverse transcriptase polymerase chain reaction
RUNX2 RUNT-related transcription factor 2 = CBFA1
SOX9 SRY-box 9
SP7 Sp7 transcription factor = osterix
TDF tendon-derived fibroblast
TGF transforming growth factor
TLR Toll-like receptor
TNF tumor necrosis factor
VEGF vascular endothelial-derived growth factor A
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List
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