Doaa Ahmed Said Helal Prof. Dr. Mohamed A. El-Nabarawi Ass.Prof. Dr. Dalia Abd El-Rahman ...

20 Pages·2015·0.14 MB·English

Pharmaceutical Study on Certain Antifungal
Drug in Topical Dosage Forms.

Thesis Presented
By
Doaa Ahmed Said Helal
Demonstrator of pharmaceutics and industrial pharmacy Faculty
of Pharmacy, Modern University for Technology and Information
(MTI)

For Partial Fulfillment of Master Degree in
Pharmaceutical Science (Pharmaceutics)

Under the Supervision of

Prof. Dr. Mohamed A. El-Nabarawi
Professor of Pharmaceutics and Industrial Pharmacy
Faculty of Pharmacy,Cairo University.

Ass.Prof. Dr. Dalia Abd El-Rahman Attia
Ass.Professor of Pharmaceutics and Industrial Pharmacy
Faculty of Pharmacy,Al Azhar University.

Dr. Sally Abd El-Halim
Lecturer of Pharmaceutics and Industrial Pharmacy
Faculty of Pharmacy, Cairo University.

Department of Pharmaceutics and Industrial Pharmacy
Faculty of Pharmacy
Cairo University

2012
Pharmaceutical Study on Certain Antifungal
Drug in Topical Dosage Forms.

Thesis Presented
By
Doaa Ahmed Said Helal

APPROVAL SHEET

Approved By:

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18/7/2012

ACKNOWLEDGMENT

I am sincerely grateful to Prof. Dr. Mohamed El-
Nabarawi, Professor of Pharmaceutics & Industrial Pharmacy,
Department of Pharmaceutics & Industrial Pharmacy, Faculty of
Pharmacy, Cairo University for his keen supervision, for planning the
work, continuous encouragement and indispensable support all over
this study.

I would like to express my deep appreciation to Ass. Prof. Dr.
Dalia Abd El-Rhman Assistant Professor of Pharmaceutics &
Industrial Pharmacy, Department of Pharmaceutics & Industrial
Pharmacy, Faculty of Pharmacy, Al Azhar University ,to whom I
owe so much for her supervision, valuable assistance, sincere
guidance and meticulous revision of all details of this thesis.

I would like to express my profound gratitude to Dr. Sally Abd
El-Halim Lecturer of Pharmaceutics & Industrial Pharmacy,
Department of Pharmaceutics & Industrial Pharmacy, Faculty of
Pharmacy, Cairo University for her kindness, continuous advice, and
continuous help.

No words express my sincerest appreciation and profound
gratitude to Prof. Dr. Alia Badawy Professor of Pharmaceutics
& Industrial Pharmacy, Department of Pharmaceutics & Industrial
Pharmacy, Faculty of Pharmacy, Cairo University for her advices.

I would like to express sincerest appreciation to Dr. Magda
Ibrahim Q.C. manager of E.I.P.I.CO. Company, for her kindness
and continuous support. Wael Ali & Akram Bashir & Ahmed
Abd El Hay for their help and their support.

Special thanks to Dr. Mahmoud Teaima Lecturer of
Pharmaceutics & Industrial Pharmacy, Department of Pharmaceutics
& Industrial Pharmacy, Faculty of Pharmacy, Cairo University for
his continuous help and his kindness.

I wish also to express my deep gratefulness and appreciation to
Mahmoud Abd El Atty Teaching Assistant of microbiology,
Department of microbiology, Faculty of Pharmacy, Misr University
for Science and Technology, for his great help and guidance in doing
the microbiological investigations.

My thanks are extended to all persons in E.I.P.I.Co. company for
their goodwill during the whole duration of this thesis
Finally, no words can express my deep gratefulness to My
Family especially My MOTHER for her continuous
encouragement and reinforcement during this work, providing all
support beyond demand.
CONTENTS
Page
ABSTRACT……………………………………..……….........I
INTRODUCTION……………………...…………..…….......1
SCOPE OF WORK……………………….…………….........9

CHAPTER I

Formulation and Evaluation of Fluconazole Topical
Gels.

INTRODUCTION………………………………………..10
EXPERIMENTAL………………………………….......15
RESULTS AND DISCUSSION………………………..24
CONCLUSION……………………………………........70

CHAPTER II

Formulation and Evaluation of Fluconazole Topical
Emulgels.

INTRODUCTION……………………………………......72
EXPERIMENTAL……………………………..............74
RESULTS AND DISCUSSION……………………….80
CONCLUSION…………………………………...…...121
CHAPTER III

Formulation and Evaluation of Fluconazole Topical
Microemulsions.

INTRODUCTION…………………………………………123
EXPERIMENTAL……………………………………….141
RESULTS AND DISCUSSION………………………....146
CONCLUSION…………………………………………..169

APPENDIX......................................................................171

REFERENCES…………………………………............174

صخل ملا ى برع لا…………………………………….........192
List of Tables

Table (1):Composition of Fluconazole Topical Gels………….....19

Table (2):Physical Properties of Fluconazole Topical Gels……...39

Table (3): Rheological properties of Fluconazole Topical Gel
formulae...........……………………… ………….........………..42

Table (4): In vitro drug release data of Fluconazole from different
Gel formulae............................................……………….......... 51

Table (5): kinetics analysis of In-Vitro Release of Fluconazole
from gel formulae……………………….....……………………64

Table (6): in vitro Permeation Study of fluconazole from the
Selected Topical Gel formulae...................................................67

Table(7):the inhibition zone of the prepared gels………………..68

Table (8):Composition of different emulgel formulae………...…82

Table (9): the evaluation of Fluconazole Topical Emulgel
formulae………………………….........……………………..….83

Table (10): Rheological properties of Fluconazole Topical
emulgels………....................……………………………………89
Table (11):In vitro release of fluconazole from emulgel
formulae……….................…………………………………..….92

Table (12): Kinetics analysis of In vitro release of drug from
emulgel formulae......................………………………………….115

Table (13): in vitro permeation study of fluconazole from different
emulgel formulae........................................................................117

Table (14):The inhibition zone of the prepared emulgels………119

Table (15): The solubility of Fluconazole in various oil, surfactant
and co-surfactant........…………………………………………146

Table(16):The Composition of Microemulsion systems..............147

Table (17): Composition of the selected ME samples from S 1,S 2,
S 3 and S 4 (%w/w)...........…………………………………....148

Table (18): Physical Properties of Fluconazole topical
Microemulsion formulae………………………………....…....150

Table (19): Rheological properties of Fluconazole topical
Microemulsion formulae……………………….........................152

Table (20): in vitro drug release data from microemulsion of
System 1………….......................................................................158