Differentiating human and nonhuman sources of Escherichia coli by phenotypic and genotypic methods

156 Pages·1997·1.6 MB·English

by  Parveen, Salina

DIFFERENTICAOTIUNBGYHPUHMEANNOTAYNPIDCNAONNDHUGEMNAONTYSPOIUCRMCEESTHOOFDESSCHERICHIA
by
SAUNAPARVEEN
AOFDTIHSESEOURFNTITAVHTEEIROSRNIEDTQPOYURCIEOTRSFOEERFNMLTEOOENFRDTISPTDHAOFIOLITRNOHSEPTOAHGPRERHTDAIYEADLGURAFETUELEFOSIFLCLHMOEONLT
UNIVERSITYOFFLORIDA
1997
Dediwchatoedarteonmoylopnagreerntisn,twhiisthworrelgdards,
ACKNOWLEDGMENTS
chairpeIrwsoonulodfmliykegtroadeuxaptreecsosmmmiyttseien,cefroerthhiasngkusidtaoncDer.,MmoatrikvaLt.ioTna,mpplaitni,entceh,e
moralandfinancialsupport.Hisknowledgeandexperiencehavebeenvery
bceonmemfiitcitaele.toDrm.eGaobvreirelthBeityteoanr,s.Dr.ISaammueexltrRe.meFlayrrgarha,teDfru.ltJoamtheesmAe.mLbiendrssayo,famnyd
Dwro.ulCdheanlgs-oilWiekei,tofoerxpthreeisrsadmvyicet,hasnukgsgetsotiDorn.s,AnawnadrurelviHeuwq,ofDrm.anCuhsacrrliepst.W.I
Kaspar,Dr.KennethM.PortierandDr.JahaA.Hamidafortheirhelpful
suggestions.
IwouldliketoacknowledgeMr.KevinRobinsonandMr.JayHarrisonfor
statisticalanalysesofdata,andMr.LeeEdmiston,Dr.ToddE.HopkinsandDr.
MaapsipscrihesacteialantcAie.oinSnhttiohrelDref.yatRftooyrbeahrcetildpEi.anngSatlamylelsiascnoedlxleMpcestr.ismNaemanpntlc.eys.CM.yIHwsoiodnugcleedrefloitrkhetahnteoikrseaxadplvrsioecsegsoamntydo
Dr.PaulA.Guligforhisadviceandassistanceintheextractionandanalysisof
plasmid.
studentIwmoeumlbderlsikeoftotheexFporoedssSamfyetgyrsaetcittiuodneitnotthheeDeppasatrtamnedntproefsFeanmtilsyt,afYfoauntdh
andCommunitySciences.Thisdissertationwouldnothavebeencompleted
withouttheirencouragementandtechnicalassistance.Acknowledgmentsare
alsoextendedtothefacultyandstaffoftheDepartmentsofFoodScienceand
HumanNutritionandFamily,YouthandCommunitySciencesfortheir
assistanceandsupport.
supportIwaonudldenlickoeutroageexmpernetsstmhryouegnhdoluetssmtyhanlikfseltoongmeydufcaamtiiloynafloretnhdeeiarvloorvse.,
Finally,Iwouldliketothankmyhusband,Mohammad,andson,Sakib,fortheir
patience,understanding,andmoralsupportthroughalltheseyearsofstudies.
ALICSKTNOOFWTLAEBDLGESMENTS viiiii
issls
IofONA
SUMMARYANDCONCLUSIONS 120
REFERENCES 123
BIOGRAPHICALSKETCH 139
LISTOFTABLES
Table Page
1. Pseorucrecnet(aNgHeSoIfi£solcaotleishruemsiasntasntoutrocean(tiHbSi)otaincds(nAoNnEhRuRmIan 49
2. Pfarrneoddmaohnmiuimnmaaalnntfesaconetusirbcieot(iHcSrIe,snisotnahncuemapanttseorunrsceof(£NHSc)o,lihiusomlaant'ed, 50
3. Percentageof£collatdifferentMARindices 50
4. sMoaujrocrefa(tNtHySa)ciidssoloaftehsumansouroeIHS)andnonhuman 60
56.. iiNNssuuoommllaabbtteeeerrssssiinnoodffdiifhhfffuueemrmreeaannnnttcsssluooubuus-rrtgccerereso((uHHpSSs))aannddnnoonnhhuummaannssoouurrccee((NNHHSS)) .6654
7. O(-NSHeSr)oigsrooluaptessofhumansource(HS)andnonhumansource 70
8. P(eNrHcSe)nt£agceoloifishoulamtaensastousrecleec(tHeSd)siamnidlarniotnyhinudmiacens.source 76
91.0.wN(PiNFutHGmhSEb),ied£rFenAotcMfiocElRai,lTissaMonAoldfaRtOhes-ausnmaedtarsnoRegTlsreoopcuurtropefcsdielose(fisHmhSil)uamraiantndyisnnooduinrchceuesm(aHnS)soisuorlcaetes..9757
11.iPsFoGlEat,esFwAiMtEh,idaenndticOa-lseMrAoRgroaunpdsRoTfpnroofnihleusmansource(NHS) .96
12.Plasmidprofilesofantibioticresistantisolates 99
Pi(srNoeHldSao)tmeidinnfatrnhotemaRhnBtuiNmbEiaoRntRiscoruersciest(aHnSc)eapnatdtenronnshoufmEa.ncosloiurce
PercentageofRBNERRisolatesatdifferentMARindices.
LISTOFFIGURES
MMRNaaeatjsjioeoorrrnvaffeleeaaIEttAsuutNrruEeeassRrRioaInfnedtRhseeasmAeppaalrlecahcsihRtieecssoelorafv.eNt.aht(eiAoANnpEaaRllRaEIcshtiucaorlianeResearch 3320
MEsatjuoarrifneeatRuerseesaarncdhsRaemsperlveesi(tReBsNoEfRtRhIeRookeryBayNational 35
D(liHenSnk)dagr£eogcaronlaailmyissoiofslaatnetisbdioettiecrrmeisniesdtabnyceEupcrloifidlieasnfmoertrhiuc,maanvesroaugrece 52
D(lNeiHnnSkdaIrgoe£garncaaomlliysoiifssoalnattiebsiodteicterremsiinsetadncbeypEruocfliildeisafnomrentroinch,uamvaenrasgoeurce54
Tprwion-cdiipmaeln-scioomnpoanlenpltotanoaflyasnitsibioticresistancepatternsby 56
DneonndhruomgarnamsooufrFceAMINEHSpr)of£ilecsoloifishoulamtaesnsource(HS)and 62
TRRgewTesosnt-red(irBica)tmtieoeonfdnshbieuyonmndpaoarlnniunpccsllioopeutaarolscf-eecFo[(AmHHipSMno)Edni£eWpn)rtcodofailiniglaeelissystsooilf(saA.t£)esacnoldiriesporleatseesntative..7636
RReTsstroifct<iBo)noefnndoonnuhculmeaanseso(Mu/rn<c/elll(|NdHiSgIes£t(cAo)liainsdolrateepsr.e.sentative 74