DIFFERENTICAOTIUNBGYHPUHMEANNOTAYNPIDCNAONNDHUGEMNAONTYSPOIUCRMCEESTHOOFDESSCHERICHIA by SAUNAPARVEEN AOFDTIHSESEOURFNTITAVHTEEIROSRNIEDTQPOYURCIEOTRSFOEERFNMLTEOOENFRDTISPTDHAOFIOLITRNOHSEPTOAHGPRERHTDAIYEADLGURAFETUELEFOSIFLCLHMOEONLT UNIVERSITYOFFLORIDA 1997 Dediwchatoedarteonmoylopnagreerntisn,twhiisthworrelgdards, ACKNOWLEDGMENTS chairpeIrwsoonulodfmliykegtroadeuxaptreecsosmmmiyttseien,cefroerthhiasngkusidtaoncDer.,MmoatrikvaLt.ioTna,mpplaitni,entceh,e moralandfinancialsupport.Hisknowledgeandexperiencehavebeenvery bceonmemfiitcitaele.toDrm.eGaobvreirelthBeityteoanr,s.Dr.ISaammueexltrRe.meFlayrrgarha,teDfru.ltJoamtheesmAe.mLbiendrssayo,famnyd Dwro.ulCdheanlgs-oilWiekei,tofoerxpthreeisrsadmvyicet,hasnukgsgetsotiDorn.s,AnawnadrurelviHeuwq,ofDrm.anCuhsacrrliepst.W.I Kaspar,Dr.KennethM.PortierandDr.JahaA.Hamidafortheirhelpful suggestions. IwouldliketoacknowledgeMr.KevinRobinsonandMr.JayHarrisonfor statisticalanalysesofdata,andMr.LeeEdmiston,Dr.ToddE.HopkinsandDr. MaapsipscrihesacteialantcAie.oinSnhttiohrelDref.yatRftooyrbeahrcetildpEi.anngSatlamylelsiascnoedlxleMpcestr.ismNaemanpntlc.eys.CM.yIHwsoiodnugcleedrefloitrkhetahnteoikrseaxadplvrsioecsegsoamntydo Dr.PaulA.Guligforhisadviceandassistanceintheextractionandanalysisof plasmid. studentIwmoeumlbderlsikeoftotheexFporoedssSamfyetgyrsaetcittiuodneitnotthheeDeppasatrtamnedntproefsFeanmtilsyt,afYfoauntdh andCommunitySciences.Thisdissertationwouldnothavebeencompleted withouttheirencouragementandtechnicalassistance.Acknowledgmentsare alsoextendedtothefacultyandstaffoftheDepartmentsofFoodScienceand HumanNutritionandFamily,YouthandCommunitySciencesfortheir assistanceandsupport. supportIwaonudldenlickoeutroageexmpernetsstmhryouegnhdoluetssmtyhanlikfseltoongmeydufcaamtiiloynafloretnhdeeiarvloorvse., Finally,Iwouldliketothankmyhusband,Mohammad,andson,Sakib,fortheir patience,understanding,andmoralsupportthroughalltheseyearsofstudies. ALICSKTNOOFWTLAEBDLGESMENTS viiiii issls IofONA SUMMARYANDCONCLUSIONS 120 REFERENCES 123 BIOGRAPHICALSKETCH 139 LISTOFTABLES Table Page 1. Pseorucrecnet(aNgHeSoIfi£solcaotleishruemsiasntasntoutrocean(tiHbSi)otaincds(nAoNnEhRuRmIan 49 2. Pfarrneoddmaohnmiuimnmaaalnntfesaconetusirbcieot(iHcSrIe,snisotnahncuemapanttseorunrsceof(£NHSc)o,lihiusomlaant'ed, 50 3. Percentageof£collatdifferentMARindices 50 4. sMoaujrocrefa(tNtHySa)ciidssoloaftehsumansouroeIHS)andnonhuman 60 56.. iiNNssuuoommllaabbtteeeerrssssiinnoodffdiifhhfffuueemrmreeaannnnttcsssluooubuus-rrtgccerereso((uHHpSSs))aannddnnoonnhhuummaannssoouurrccee((NNHHSS)) .6654 7. O(-NSHeSr)oigsrooluaptessofhumansource(HS)andnonhumansource 70 8. P(eNrHcSe)nt£agceoloifishoulamtaensastousrecleec(tHeSd)siamnidlarniotnyhinudmiacens.source 76 91.0.wN(PiNFutHGmhSEb),ied£rFenAotcMfiocElRai,lTissaMonAoldfaRtOhes-ausnmaedtarsnoRegTlsreoopcuurtropefcsdielose(fisHmhSil)uamraiantndyisnnooduinrchceuesm(aHnS)soisuorlcaetes..9757 11.iPsFoGlEat,esFwAiMtEh,idaenndticOa-lseMrAoRgroaunpdsRoTfpnroofnihleusmansource(NHS) .96 12.Plasmidprofilesofantibioticresistantisolates 99 Pi(srNoeHldSao)tmeidinnfatrnhotemaRhnBtuiNmbEiaoRntRiscoruersciest(aHnSc)eapnatdtenronnshoufmEa.ncosloiurce PercentageofRBNERRisolatesatdifferentMARindices. LISTOFFIGURES MMRNaaeatjsjioeoorrrnvaffeleeaaIEttAsuutNrruEeeassRrRioaInfnedtRhseeasmAeppaalrlecahcsihRtieecssoelorafv.eNt.aht(eiAoANnpEaaRllRaEIcshtiucaorlianeResearch 3320 MEsatjuoarrifneeatRuerseesaarncdhsRaemsperlveesi(tReBsNoEfRtRhIeRookeryBayNational 35 D(liHenSnk)dagr£eogcaronlaailmyissoiofslaatnetisbdioettiecrrmeisniesdtabnyceEupcrloifidlieasnfmoertrhiuc,maanvesroaugrece 52 D(lNeiHnnSkdaIrgoe£garncaaomlliysoiifssoalnattiebsiodteicterremsiinsetadncbeypEruocfliildeisafnomrentroinch,uamvaenrasgoeurce54 Tprwion-cdiipmaeln-scioomnpoanlenpltotanoaflyasnitsibioticresistancepatternsby 56 DneonndhruomgarnamsooufrFceAMINEHSpr)of£ilecsoloifishoulamtaesnsource(HS)and 62 TRRgewTesosnt-red(irBica)tmtieoeonfdnshbieuyonmndpaoarlnniunpccsllioopeutaarolscf-eecFo[(AmHHipSMno)Edni£eWpn)rtcodofailiniglaeelissystsooilf(saA.t£)esacnoldiriesporleatseesntative..7636 RReTsstroifct<iBo)noefnndoonnuhculmeaanseso(Mu/rn<c/elll(|NdHiSgIes£t(cAo)liainsdolrateepsr.e.sentative 74