Date palm biotechnology protocols. Volume I, Tissue culture applications PDF
Preview Date palm biotechnology protocols. Volume I, Tissue culture applications
Methods in Molecular Biology 1637 Jameel M. Al-Khayri S. Mohan Jain Dennis V. Johnson Editors Date Palm Biotechnology Protocols Volume I Tissue Culture Applications M M B ETHODS IN OLECULAR IO LO GY SeriesEditor JohnM.Walker School of Lifeand MedicalSciences University ofHertfordshire Hatfield, Hertfordshire,AL109AB,UK Forfurther volumes: http://www.springer.com/series/7651 Date Palm Biotechnology Protocols Volume I Tissue Culture Applications Edited by Jameel M. Al-Khayri Department of Agricultural Biotechnology King Faisal University Al-Hassa, SA S. Mohan Jain Agricultural Sciences University of Helsinki Helsinki, FL Dennis V. Johnson Cincinnati, OH, USA Editors JameelM.Al-Khayri S.MohanJain DepartmentofAgricultural AgriculturalSciences Biotechnology UniversityofHelsinki KingFaisalUniversity Helsinki,FL Al-Hassa,SA DennisV.Johnson Cincinnati,OH,USA ISSN1064-3745 ISSN1940-6029 (electronic) MethodsinMolecularBiology ISBN978-1-4939-7155-8 ISBN978-1-4939-7156-5 (eBook) DOI10.1007/978-1-4939-7156-5 LibraryofCongressControlNumber:2017945286 ©SpringerScience+BusinessMediaLLC2017 Thisworkissubjecttocopyright.AllrightsarereservedbythePublisher,whetherthewholeorpartofthematerialis concerned,specificallytherightsoftranslation,reprinting,reuseofillustrations,recitation,broadcasting,reproduction onmicrofilmsorinanyotherphysicalway,andtransmissionorinformationstorageandretrieval,electronicadaptation, computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdeveloped. 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Preface Biotechnologyisplayinganessentialroleinsustainingsufficientfoodsupplieschallengedby the burgeoning human population and current global environmental changes. Develop- ment of plant biotechnology has reached advanced stages of applications in several impor- tant food crops. Palm fruit crops, particularly those grown predominantly in developing countries, are attracting increasing research attention in response to greater funding allo- catedforplantbiotechnologyresearchinthesecountries.Cultivationofdatepalm(Phoenix dactylifera L.), an economically important tree species grown in the arid and semiarid regions of the Middle East and North Africa, has expanded to Australia, Southern Africa, South America, Mexico and the southwestern USA. In addition to its high nutritive value andversatiletreeby-products,dateshavediversemedicinalpropertiesduetoahighcontent of bioactive compounds including carotenoids, polyphenols, tannins, and sterols. These compoundsareknownforantioxidantactivity,cholesterol-loweringproperties,andchemo- preventionwhichmayinhibitcancer,diabetes,andcardiovasculardiseases. Our earlier book entitled Date Palm Biotechnology provided a comprehensive coverage onvariousbiotechnologicalaspectsinrelationtodatepalm.ThisbookDatePalmBiotech- nology Protocols is intended to supplement the previous volume and to provide precise stepwiseprotocolsinthefieldofdatepalmbiotechnology.Materials,equipment,methods, and analysis are detailed for easy adoption by novices and substantiated with relevant referencesforspecialistsdesiringtoindulgeinnewbiotechnologicalapplications. The book consists of two volumes, Vol 1: Tissue Culture Applications and Vol 2: Germplasm Conservation and Molecular Breeding. This first volume comprises 27 chapters grouped in six parts: Part I Adventitious Organogenesis; Part II Somatic Embryogenesis; Part III Contamination, Hyperhydricity, and Acclimatization; Part IV Cell Suspension, Protoplast,andBioreactors;PartVGeneticTransformation;andPartVISecondaryMeta- bolitesandAbioticStress.Eachprotocolchapterstartswithabriefintroductionrelevantto the topic methodology and then lists the necessary materials, including chemical and equipment, as well as reagent preparation, followed by detailed reproducible procedural stepssupportedwithobservationalnotesandillustrativephotographs.Thechaptersinthis book are authored and reviewed by prominent specialists demonstrating distinct research contributions to date palm biotechnology, invited from industry, universities, and research institutes. This two-volume book is a valuable resource to students, researchers, scientists, com- mercialproducers,consultants,andpolicymakersinterestedinagricultureorplantsciences particularly in date palm biotechnology. This protocol manual is highly recommended for teachingadvancedlevelundergraduateaswellasgraduatecoursesindatepalmbiotechnol- ogyorother relevantcourses. Wewouldliketoexpressoursinceregratitudeforthegenerosityandexcellentcoopera- tion of the chapter authors of these two volumes. In total, 125 scientists, representing 21 countries, have provided their detailed knowledge of the biotechnological protocols being v vi Preface employedtoimprovethedatepalmandenhanceitscontributiontotheworld’sfoodneeds. Thesetwoscientificbooksrepresenttheepitomeofinternationalcooperation,transcending thevariousdifferences,whichexistintheworld,andemphasizingthecommonsolutionsto feedingtheburgeoninghumanpopulationoftheplanetweallshare. Al-Hassa,SA JameelM.Al-Khayri Helsinki,FL S.MohanJain Cincinnati,OH,USA DennisV.Johnson Contents Preface ..................................................................... v Contributors................................................................. xi PART I ADVENTITIOUS ORGANOGENESIS 1 Cultivar-DependentDirectOrganogenesisofDatePalmfromShoot TipExplants............................................................ 3 LarbiAbahmane 2 NAA-InducedDirectOrganogenesisfromFemaleImmatureInflorescence ExplantsofDatePalm................................................... 17 HussamS.M.Khierallah,SalehM.Bader,andMakkiA.Al-Khafaji 3 DirectOrganogenesisfromImmatureFemaleInflorescenceofDatePalm byGradualReductionof2,4-DConcentration ............................. 27 EzzEl-DinG.Gadalla 4 OptimizedDirectOrganogenesisfromShootTipExplantsofDate Palm .................................................................. 37 RehabSidky 5 DirectOrganogenesisandIndirectSomaticEmbryogenesisbyInVitro ReversionofMatureFemaleFloralBudstoaVegetativeState................ 47 EmanM.M.Zayed PART II SOMATIC EMBRYOGENESIS 6 EnhancedIndirectSomaticEmbryogenesisofDatePalmUsingLow LevelsofSeawater....................................................... 63 RaniaA.Taha 7 EnhancedIndirectSomaticEmbryogenesisfromShoot-TipExplants ofDatePalmbyGradualReductionsof2,4-DConcentration................ 77 ZeinabE.Zayed 8 IndirectSomaticEmbryogenesisfromMatureInflorescenceExplants ofDatePalm ........................................................... 89 AliM.Al-Ali,Chien-YingKo,SultanA.Al-Sulaiman,SamiO.Al-Otaibi, AbdUlmoneemH.Al-Khamees,andMegahedH.Ammar 9 IndirectSomaticEmbryogenesisofDatePalmUsingJuvenileLeaf ExplantsandLow2,4-DConcentration ................................... 99 LotfiFki,WalidKriaa,AmeniNasri,EmnaBaklouti,OlfaChkir, RajaB.Masmoudi,AlainRival,andNoureddineDrira 10 Desiccation-EnhancedMaturationandGerminationofDatePalm SomaticEmbryosDerivedfromCellSuspensionCulture.................... 107 NazimBoufis,KhayreddineTitouh,andLakhdarKhelifi vii viii Contents 11 DesiccationandColdHardeningofDatePalmSomaticEmbryos ImproveGermination ................................................... 119 HusseinJ.Shareef 12 HistologicalEvidenceofIndirectSomaticEmbryogenesisfrom ImmatureFemaleDatePalmInflorescences................................ 129 EmanM.M.ZayedandOlaH.Abdelbar 13 HistologicalAnalysisoftheDevelopmentalStagesofDirectSomatic EmbryogenesisInducedfromInVitroLeafExplantsofDatePalm ........... 145 OlaH.Abdelbar PART III CONTAMINATION, HYPERHYDRICITYAND ACCLIMATIZATION 14 IdentifyingandControllingContaminationofDatePalmTissue Cultures ............................................................... 165 AbeerH.I.Abdel-Karim 15 ControllingHyperhydricityinDatePalmInVitroCulturebyReduced ConcentrationofNitrateNutrients ....................................... 175 MaiadaM.El-DawayatiandZeinabE.Zayed 16 ImprovementofInVitroDatePalmPlantletAcclimatizationRate withKinetinandHoaglandSolution ...................................... 185 MonaM.Hassan PART IV CELL SUSPENSION, PROTOPLASTAND BIOREACTORS 17 PlantRegenerationfromSomaticEmbryogenicSuspensionCultures ofDatePalm........................................................... 203 MansourA.Abohatem,YazidBakil,andMohmmedBaaziz 18 SynchronizationofSomaticEmbryogenesisinDatePalmSuspension CultureUsingAbscisicAcid.............................................. 215 HussainA.Alwael,PoornanandaM.Naik,andJameelM.Al-Khayri 19 MicrocalliInductioninProtoplastsIsolatedfromEmbryogenicCallus ofDatePalm........................................................... 227 Khayreddine Titouh,NazimBoufis,andLakhdarKhelifi 20 TemporaryImmersionSystemforDatePalmMicropropagation.............. 239 AhmedOthmani,ChokriBayoudh,AmelSellemi,andNoureddineDrira 21 PlantformBioreactor forMassMicropropagationofDatePalm .............. 251 AbdulminamH.A.Almusawi,AbdullahJ.Sayegh,AnsamM.S.Alshanaw, andJohnL.GriffisJr PART V GENETIC TRANSFORMATION 22 GeneticTransformationofDatePalmViaMicroprojectile Bombardment.......................................................... 269 MousaMousavi,AmirMousavi,AliA.Habashi,KazemArzani, BaharehDehsara,andMohsenBrajeh Contents ix 23 MicroprojectileBombardmentTransformationofDatePalm UsingtheInsecticidalCholesterolOxidase(ChoA)Gene .................... 281 MaiA.AllamandMahmoudM.Saker 24 TransientGUSGeneExpressioninDatePalmFruitUsingAgroinjection TransformationTechnique............................................... 295 MoheiEl-DinM.Solliman,HebaallahA.Mohasseb, AbdullatifA.Al-Khateeb,JameelM.Al-Khayri, andSulimanA.Al-Khateeb PART VI SECONDARY METABOLITES AND ABIOTIC STRESS 25 BioreactorSteroidProductionandAnalysisofDatePalm EmbryogenicCallus..................................................... 309 SherifEl-SharabasyandMaiadaEl-Dawayati 26 ExtractionandEstimationofSecondaryMetabolitesfromDate PalmCellSuspensionCultures ........................................... 319 PoornanandaM.NaikandJameelM.Al-Khayri 27 InVitroAssessmentofAbioticStressinDatePalm:Salinity andDrought........................................................... 333 JameelM.Al-Khayri,PoornanandaM.Naik,andHussainA.Alwael Index...................................................................... 347 Contributors LARBIABAHMANE (cid:1) PlantBiotechnologyLaboratory,INRA,MarrakechRegionalCenter ofAgriculturalResearch,Marrakech,Morocco ABEERH.I.ABDEL-KARIM (cid:1) CentralLaboratoryofDatePalmResearchandDevelopment, AgriculturalResearchCenter,Giza,Egypt OLAH.ABDELBAR (cid:1) DepartmentofAgriculturalBotany,FacultyofAgriculture,AinShams University,Cairo,Egypt MANSOURA.ABOHATEM (cid:1) DepartmentofBiology,FacultyofEducationandLanguages, AmranUniversity,Amran,Yemen;PlantTissueCultureLaboratory,PublicCorporation forAgriculturalServices,MinistryofAgricultureandIrrigation,Sana´a,Yemen ALIM.AL-ALI (cid:1) PlantTissueCultureandGeneticEngineeringLaboratory,National AgricultureandAnimalResourcesResearchCenter,MinistryofEnvironment,Waterand Agriculture,Riyadh,SaudiArabia MAKKIA.AL-KHAFAJI (cid:1) DepartmentofHorticultureandLandscapeGardening, CollegeofAgriculture,UniversityofBaghdad,Baghdad,Iraq ABDULMONEEMH.AL-KHAMEES (cid:1) PlantTissueCultureandGeneticEngineering Laboratory,NationalAgricultureandAnimalResourcesResearchCenter,Ministryof Environment,WaterandAgriculture,Riyadh,SaudiArabia ABDULLATIFA.AL-KHATEEB (cid:1) DepartmentofAgriculturalBiotechnology,Collegeof AgricultureandFoodSciences,KingFaisalUniversity,Al-Hassa,SaudiArabia SULIMANA.AL-KHATEEB (cid:1) DepartmentofEnvironmentandNaturalResources, CollegeofAgricultureandFoodSciences,KingFaisalUniversity,Al-Hassa,SaudiArabia JAMEELM.AL-KHAYRI (cid:1) DepartmentofAgriculturalBiotechnology,CollegeofAgriculture andFoodSciences,KingFaisalUniversity,Al-Hassa,SaudiArabia MAIA.ALLAM (cid:1) GeneticEngineeringandBiotechnologyDivision,DepartmentofPlant Biotechnology,NationalResearchCenter,Giza,Egypt ABDULMINAMH.A.ALMUSAWI (cid:1) CellandBiotechnologyResearchUnit,CollegeofScience, BasraUniversity,Basra,Iraq SAMIO.AL-OTAIBI (cid:1) PlantTissueCultureandGeneticEngineeringLaboratory,National AgricultureandAnimalResourcesResearchCenter,MinistryofEnvironment,Waterand Agriculture,Riyadh,SaudiArabia ANSAMM.S.ALSHANAW (cid:1) DatePalmResearchCentre,BasraUniversity,Basra,Iraq SULTANA.AL-SULAIMAN (cid:1) PlantTissueCultureandGeneticEngineeringLaboratory, NationalAgricultureandAnimalResourcesResearchCenter,MinistryofEnvironment, WaterandAgriculture,Riyadh,SaudiArabia HUSSAINA.ALWAEL (cid:1) DepartmentofAgriculturalBiotechnology,CollegeofAgricultureand FoodSciences,KingFaisalUniversity,Al-Hassa,SaudiArabia MEGAHEDH.AMMAR (cid:1) PlantProductionDepartment,CollegeofFoodandAgriculture Sciences,KingSaudUniversity,Riyadh,SaudiArabia KAZEMARZANI (cid:1) DepartmentofHorticultureScience,FacultyofAgriculture,Tarbiat ModaresUniversity,Tehran,Iran MOHMMEDBAAZIZ (cid:1) LaboratoiredeBiotechnologies-Biochimie,ValorisationetProtectiondes Plantes,Faculte´ desSciencesSemlalia,Universite´ CadiAyyad,Marrakech,Morocco xi
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