Cytochrome P450, Part B

494 Pages·1996·10.119 MB·English

by  Eric F. Johnson and Michael R. Waterman (Eds.)

Contributors to Volume 272
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
JOHN F. ANDERSEN (34), Department of Ento- ALEXIS BRONINE (6), Centre de GdnOtique
mology, University of Arizona, Tucson, Ar- Mol&ulaire, Centre National de al Re-
izona 85721 cherche Scientifique, 91198 Gif-sur-yvette
TOMMY ANDERSSON (14), Clinical Pharma- Cedex, France
cology, Astra Hiissle AB, S-43183 MOn- KIN BR~SEN (20), Department of Clinical
dal, Sweden Pharmacology, Institute of Medical Biol-
HENRY J. BARNES (1), Immune Complex Cor- ogy, Odense University, DK-5000 Odense,
poration, La Jolla, California 92038 Denmark
PHILLIPE BEAUNE (8), CHU NECKER-IN- GNAIQ-NIJ CHEN (41), Department of Environ-
SERM U ,57 Biochimie Pharmacologique mental Health Sciences', Johns Hopkins
et Mdtabolique, Universit~ Rend Descartes, University School of Public Health, Balti-
F-75730 "siraP Cedex ,51 France more, Maryland 21218
CLAIRE BELLOC (8), CHU NECKER-IN- YOUNG-JIN CHUN (4), Toxicology Research
SERM U ,57 Biochimie Pharmacologique Center, Korean Research Institute of Chem-
et Mdtabolique, Universitd Rend Descartes, ical Technology, Taejon, South Korea
F-75730 "siraP Cedex ,51 France
MINOR J. COON (3), Department of Biological
IRENE ETSINEVNEB (29), Department of Cellu-
Chemistry, University of Michigan Medical
lar and Molecular Enzymology, Plant Mo-
School, Ann Arbor, Michigan 48109
lecular Biology Institute, CNRS, Stras-
RODNEY CROTEAU (27), Department of Ge-
bourg, France
netics and Cell Biology, Institute of Biologi-
FRANCOIS BERTHOU (12), Labs Biochimie-
cal Chemistry, Washington State ,yti'srevinU
Nutrition, Facultd de Mddecine, F-29285
Pullman, Washington 99164
Brest, France
ANN K. DALY (22), Department of Pharmaco-
DONALD J. BIRKETr (14, 15), Department of
logical Sciences, University of Newcastle
Clinical Pharmacology, Flinders Urliversity
upon Tyne Medical School Framlington
of South Australia, Flinders Medical Center,
Place, Newcastle upon Tyne NE2 4HH,
Bedford Park, South Australia 5042,
United Kingdom
Australia
JAMES J. DE VOSS (37), Department of Phar-
JovcE BLAISDZLL (23), Laboratory "fo Bio-
maceutical Chemistry, School of Pharmacy,
chemical Risk Analysis', National Institute
University of California, San Francisco,
of Environmental Health Sciences, Re-
California 94143
search Triangle Park, North Carolina 27709
SIGRIO BOTSCH (10), Dr. Margarete Fischer- ,CNAHS ARA S. DEHAL (17), Worcester Foun-
Bosch-lnstitate far Klinische Pharmakolo- dation for Biomedical Research, Shrews-
gie, D-70341 Stuttgart, Germany bury, "sttesuhcassaM 01545
ALAN R. BRASH (28), Department of Pharma- SNEZANA DJORDJEV1C (40), Department of
cology, Vanderbilt University Medical Cen- Biochemistry, Center for Advanced BiD-
ter, Nashville, Tennessee 37232 technology and Medicine, Piscataway, New
Douwz D. BREIMER (19), Division of Phar- Jersey 08854
macology, Leiden/Amsterdam Center for HTENNEK DORKO (42), Department of Pathol-
Drug Research, University of Leiden, 2300 ogy, University of Pittsburgh, Pittsburgh,
RA Leiden, The "sdnalrehteN Pennsylvania 15261
X CONTRIBUTORS TO VOLUME 272
FRANCIS DURST (29), Department of Cellular FRANK J. GONZALEZ (44), Division of Basic
and Molecular Enzymology, Plant Molecu- Science, National Cancer Institute, National
lar Biology Institute, CNRS, Strasbourg, Institutes of Health, Bethesda, Maryland
France 20892
RONALD W. ESTABROOK (2, 5), Department SANDRA E. GRAHAM-LORENCE (35), Depart-
of Biochemistry, The University of Texas ment of Biochemistry, The University of
Southwestern Medical Center, Dallas, Texas Southwestern Medical Center, Dallas,
Texas 75235 Texas 75235
KAREN S. FAIRBROTHER (22), Department of KEES GROEN (19), Department of Clinical
Pharmacological Sciences, University of Pharmacokinetics, Janssen Research Foun-
Newcastle upon Tyne Medical School, dation, B-2340 Beerse, Belgium
Framlington Place, Newcastle upon Tyne
NE2 4HH, United Kingdom F. PETER GUENGERICH (4), Department of
Biochemistry and Center in Molecular Toxi-
MICHAEL J. FASCO (43), New York State De-
cology, Vanderbilt University School of
partment of Health, Wadsworth Center, Al-
Medicine, Nashville, Tennessee 37232
bany, New York 12201
ZuYu Guo (4), Rhone-Poulenc-Rorer, De-
KEVIN M. FAULKNER (5), Department of Bio-
partment of Drug Metabolism, Collegeville,
chemistry, The University of Texas South-
Pennsylvania 19426
western Medical Center, Dallas, Texas
75235 BARBARA ANN HALKIER (30), Department of
PEDRO M. FERNANDEZ-SALGUERO (44), Divi- Plant Biology, Plant Biochemistry Labora-
sion of Basic Science, National Cancer Insti- tory, Royal Veterinary and Agricultural
tute, National Institutes of Health, Bethesda, University, D K-1871 Frederiksberg ,C
Maryland 20892 Denmark
RENE FEYEREISEN (34), Department of Ento- IHCI-N1HS HAYASHI (25), Department of Bio-
mology, University of Arizona, Tucson, Ar- chemistry, Saitama Cancer Center Research
izona 85721 Institute, Saitama 362, Japan
CHARLES W. FISHER (2, 5), Department of JERRY HEVNER (27), Department of Genetics
Biochemistry, The University of Texas and Cell Biology, Institute of Biological
Southwestern Medical Center, Dallas, Chemistry, Washington State University,
Texas 75235 Pullman, Washington 99164
YOSHIHIKO FUNAE (18), Laboratory of Chem- GEORG HEINKELE (10), Dr. Margarete Fi-
istry, Osaka City University Medical School, scher-Bosch-Institute fiir Klinische Phar-
Osaka 545, Japan makologie, D-70341 Stuttgart, Germany
ALINE GAUFFRE (8), CHU NECKER-IN- TIMOTHY A. HOLTON (31), Florigene Pty.
SERM U ,57 Biochimie Pharmacologique Ltd., Collingwood, Victoria 3066, Australia
et Mdtabolique, Universitd Rend Descartes,
F-75730 Paris Cedex ,51 France JEFVREY R. IDLE (22), Faculty of Medicine,
Institute of Cancer Research and Molecular
JEAN-CHARLES GAUTIER (8), CHU
Biology, The University of Trondheim,
NECKER-INSERM U ,57 Biochimie Phar-
N-7005 Trondheim, Norway
macologique et Mdtabolique, Universitd
Rend Descartes, F-75730 Paris Cedex ,51 MAGNUS INGELMAN-SUNDBERG (24), Institute
France of Environmental Medicine, Karolinska In-
stitute, S-171 77 Stockholm, Sweden
JOYCE A. GOLDSTEIN (23), Laboratory of Bio-
chemical Risk Analysis, National Institute JEFFREY P. JONES (36), Department of Phar-
of Environmental Health Sciences, Re- macology and Physiology, University of
search Triangle Park, North Carolina 27709 Rochester, Rochester, New York 14642
CONTRIBUTORS TO VOLUME 272 xi
THOMAS JURETZEK (7), Department of Cell JOHN I. MANCHESTER (38), Environmental
Biology, Max Delbrack Center for Molecu- Molecular Sciences Laboratory, Pacific
lar Medicine, D-13125 Berlin, Germany Northwest National Laboratories, Rich-
WEANER KALOW (13), Department of Phar- land, Washington 99352
macology, University of Toronto, Toronto, MARTHA V. MARTIN (4), Department of BiD-
Ontario M5S 1A8, Canada chemistry and Center in Molecular Toxicol-
LAURENCE KAMINSKY (43), New York State ogy, Vanderbilt University School of Medi-
Department of Health, Wadsworth Center, cine, Nashville, Tennessee 37232
Albany, New York 12201 BETTIE SUE SILER MASTERS (40), Department
KANAME KAWAJIRI (25), Department of -DiB of Biochemistry, The University of Texas
chemistry, Saitama Cancer Center Research Health Science Center, San Antonio,
Institute, Saitama 362, Japan Texas 78284
JOHN L. MCCARTHY (33), Department of Bio-
JUNG-JA PARK KIM (40), Department of -DiB
logical Sciences, Southern Methodist Uni-
chemistry, Medical College of Wisconsin,
versity, Dallas, Texas 75275
Milwaukee, Wisconsin 53226
SIO~fNARF-NAEJ MENEZ'~ (12), Labs Bio-
HTENNEK R. KORZEKWA (36), Center for Clin-
chimie-Nutrition, Facultd de Mddecine,
ical Pharmacology, University of Pitts-
F-29285 Brest, France
burgh, Pittsburgh, Pennsylvania 15213
ALFRED H. MERRILL, JR. (41), Department of
THOMAS A. KOST (9), Division of Biochemis-
Biochemistry, Emory University School of
try, Glaxo-Wellcome, Inc., Research Trian-
Medicine, Atlanta, Georgia 30322
gle Park, North Carolina 27709
JOHN O. MINERS (14, 15), Department of Clin-
HErO K. KROEMER (10), Dr. Margarete Fi-
ical Pharmacology, Flinders Medical Cen-
scher-Bosch-Institute far Klinische Phar-
tre, BedJbrd Park, South Australia 5042,
makologie, D-70341 Stuttgart, Germany
Australia
DAVID KUPFER (17), Worcester Foundation
BIRGER LINDBERG MOLLER (30), Department
for Biomedical Research, Shrewsbury, Mas-
of Plant Biology, Plant Biochemistry Labo-
sachusetts 01545
ratory, Royal Veterinary and Agricultural
SzE-ME! CINDY LAI.I (26), Central Research University, DK-1871 Frederiksberg ,C
and Development, The Dupont Company Denmark
Experimental Station, Wilmington, Dela-
EDWARD T. MORGAN (41), Department of
ware 19880
Pharmacology, Emory University School of
ENIAVLYS LECOEUR (8), CHU NECKER-IN- Medicine, Atlanta, Georgia 30322
SERM U ,57 Biochimie Pharmacologique
VESSELA NEDEECHEVA (24), Center oflndus-
et Mdtabolique, Universitd Rend Descartes,
trial Hygience and Occupational Diseases,
F-75730 Paris Cedex ,51 France
National Institute of Public Health, Praha,
CAROLINE A. LEE (9), Agouron Pharmaceuti- Czech Republic
"slac Inc., San Diego, California 92121 KARIN KRAMER NIELSEN (20), Department of
DIANE R. LESTER (31), Florigene Pty. Ltd., Clinical Pharmacology, Institute of Medical
Collingwood, Victoria 3066, Australia Biology, Odense University, DK-5000 Ode-
BENEDICTE LOUERAT (6), Centre de Gdn- nse, Denmark
dtique Moldculaire, Centre National de al MARIANA NIKOLOVA-KARAKASHIAN (41),
Recherche Scientifique, 91198 Gif-sur- Department of Biochemistry, Emory Uni-
yvette Cedex, France versity School of Medicine, Atlanta, Geor-
DANIELE LUCAS (12), Labs Biochimie-Nutriti- gia 30322
tion, Facultd de Mddecine, F-29285 Brest, DANIEL P. O'KEEFE (26), Central Research
France and Development, The Dupont Company
Experimental Station, Wilmington, Dela-
? Deceased. ware 19880
xii CONTRIBUTORS TO VOLUME 272
RICK L. ORNSTEIN (38), Environmental Mo- ERIN D. SCHUETZ (42), Department of Phar-
lecular Sciences Laboratory, Pacific North- maceutical Sciences, .tS Jude Children's
west National Laboratory, Richland, Wash- Research Hospital, Memphis, Tennessee
ington 99352 38105
PAUL R. ORTIZ ED MONTELLANO (37), De- JOHN D. SCHUETZ (42), Department of Phar-
partment of Pharmaceutical Chemistry, maceutical Sciences, .tS Jude Children's Re-
School of Pharmacy, University of Califor- search Hospital, Memphis, Tennessee 38105
nia, San Francisco, California 94143
WOLF-HAGEN SCHUNCK (7), Department of
MARK D. PAULSEN (38), Environmental Mo-
Cell Biology, Max Delbrack Center for
lecular Sciences Laboratory, Pacific North- Molecular Medicine, D-13125 Berlin,
west National Laboratory, Richland, Wash-
Germany
ington 99352
JEFFREY G. Scoa~r (32), Department of Ento-
NEVETS J. PERNECKY (3), Department of
mology, Cornell University, Ithaca, New
Chemistry, Eastern Michigan University,
York 14853
Ypsilante, Michigan 48197
JULIE A. SCOTT (34), Department of Entomol-
IRENE PERSSON (24), Institute of Environmen-
ogy, University of Arizona, Tucson, Ari-
tal Medicine, Karolinska Institute, S-171 77
zona 85721
Stockholm, Sweden
JULIAN A. PETERSON (35), Department of Bio- COSEqTE J, SERABJIT-SINGH (9), Division of
chemistry, The University of Texas South- BioAnalysis and Drug Metabolism, Glaxo-
western Medical Center, Dallas, Texas Wellcome, Inc., Research Triangle Park,
53257 North Carolina 27709
LIUBOMIR A. PISAROV (42), Department of THOMAS M. SHEA (40), Department of Bio-
Pathology, University of Pittsburgh, Pitts- chemistry, The University of Texas Health
burgh, Pennsylvania 15261 Science Center, San Antonio, Texas 78284
DENIS POMPON (6), Centre de Gdn~tique Mo- MANJUNATH S. SHET (2, 5), Department of
l~culaire, Centre National de la Recherche Biochemistry, The University of Texas
Scientifique, 91198 Gif-sur-yvette Cedex, Southwestern Medical Center, Dallas,
France Texas 75235
THOMAS L. POULOS (39), Departments of Bio-
OLE SIBBESEN (30), Department of Plant Biol-
chemistry and Molecular Biology and Phys-
ogy, Plant Biochemistry Laboratory, Royal
iology Biophysics, University of California,
Veterinary and Agricultural University,
Irvine, California 92717
DK-1871 Frederiksberg ,C Denmark
DAVID L. ROBERTS (40), Department of Bio-
ERIK SKJELBO (20), Department of Clinical
chemistry, Medical College of Wisconsin,
Pharmacology, Institute of Medical Biol-
Milwaukee, Wisconsin 53226
ogy, Odense University, DK-5000 Odense,
A. DAVID RODRIGUES (21), Drug Metabolism
Denmark
Department, Abbott Laboratories, Abbott
Park, Illinois 60064 TIMOTHY J. SLITER (33), Department of Bio-
logical Sciences, Southern Methodist Uni-
MICHEL SCHALK (29), Department of Cellular
versity, Dallas, Texas 75275
and Molecular Enzymology, Plant Molecu-
lar Biology Institute, CNRS, Strasbourg, MARK J. SNYDER (34), Department of Ento-
France mology, University of Arizona, Tucson, Ar-
ULRICH SCHELLER (7), Department of Cell Bi- izona 85721
ology, Max Delbrtick Center for Molecular WENCHAO SONG (28), Department of Phar-
Medicine, D-13125 Berlin, Germany macology and Center for Experimental
MONIKA SCHICK (10), Dr. Margarete Fischer- Therapeutics, Stellar-Chance Laboratories,
Bosch-Institute fiir Klinische Pharmakolo- University of Pennsylvania, Philadelphia,
gie, D-70341 Stuttgart, Germany Pennsylvania 19104
CONTRIBUTORS TO VOLUME 272 xiii
VIDAR M. STEEN (22), Dr. Einar Martens Re- EPPIL1HP URBAN (6), Centre de G~n~tique Mo-
search Group for Biological Psychiatry, ,erialuc~l Centre National de al Recherche
Center for Molecular Medicine, Haukeland Scientifique, 91198 Gif-sur-yvette Cedex,
Hospital, N-5021 Bergen, Norway France
THOMAS WAELE (16), Department of Cell and
NEHPETS C. STROM (42), Department of Pa-
Molecular Pharmacology and Experimen-
thology, University of Pittsburgh, Pitts-
tal Therapeutics, Medical University of
burgh, Pennsylvania 15261
South Carolina, Charleston, South Caro-
KOMSUN SUDHIVORASETH (33), Department lina 29425
of Biological Sciences, Southern Methodist MIUG WANG (40), Department of Biochemis-
University, Dallas, Texas 75275 try, Medical College of Wisconsin, Milwau-
kee, Wisconsin 53226
EKUSONIE TANAKA (18), Institute of Commu-
JUNKO WATANABE (25), Department of Bio-
nity Medicine, University of Tsukuba, Tsu-
chemistry, Saitama Cancer Center Research
kuba 305, Japan
Institute, Saitama 362, Japan
BtNG-Kou TANG (13), Department of Phar- PETER J. WEDLUND (11), College of Phar-
macology, University of Toronto, Toronto, ,'2cam University of Kentucky, Lexington,
Ontario M5S 1A8, Canada Kentucky 40536
DANIEEE WERCK-REICHHART (29), Depart-
ASS1LEM T. THOMPSON (42), Department of
ment of Cellular and Molecular Enzymol-
Pathology, University of Pittsburgh, Pitts-
ogy, Plant Molecular Biology Institute,
burgh, Pennsylvania 15261
CNRS, Strasbourg, France
REHPOTSIRHC TREANOR (43), New York State GRANT R. WILKINSON (ll), Department of
Department of Health, Wadsworth Center, Pharmacology, Vanderbilt University,
Albany, New York 12201 Nashville, Tennessee 37232
Preface
The study of cytochrome P450s continues to grow at a rapid pace. The
Preface to Volume 206 of Methods in Enzymology on Cytochrome P450,
published in 1991, noted 160 distinct P450s largely of mammalian origin. A
quick check at this time indicates a count of 477 P450 sequences distributed
among 127 subfamilies comprising 70 families of which only 26 and 14,
respectively, are mammalian. This reflects a rapid increase in the identifica-
tion and characterization of plant, insect, and microbial P450s made possible
by the techniques of molecular biology.
This volume includes several chapters that focus on methods for the
investigation of P450s in these areas as well as on advances in general
techniques for the heterologous expression of P450s from cloned sequences.
These approaches have also expanded our knowledge of the human P450
enzymes, and a number of chapters that address the enzymatic characteriza-
tion of variations among individuals in the expression of the human en-
zymes, as well as procedures for characterizing associated genotypes, are
included. Moreover, three-dimensional structures are now available for
three additional P450s (compared to the single structure available in 1991),
providing an understanding of conserved features of P450s and significant
refinements in the prediction of P450 structures and substrate specificities.
Once again we have asked contributors to provide their detailed experi-
mental procedures, and they did so with enthusiasm and promptness. We
hope readers will find this compilation of protocols useful in their work.
We thank the authors for their contributions and the staff of Academic
Press for their help in the preparation of this volume.
ERIC F. JOHNSON
MICHAEL R. WATERMAN
VX
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by YENDIS P. KCIWOLOC DNA NAHTAN 0. NALPAK
EMULOV II. Preparation and Assay of Enzymes
Edited by YENDIS P. KCIWOLOC DNA NAHTAN 0. NALPAK
EMULOV III. Preparation and Assay of Substrates
Edited by YENDIS P. KClWOLOC DNA NAHTAN 0. NALPAK
EMULOV IV. Special Techniques for the Enzymologist
Edited by YENDIS P. KC1WOLOC DNA NAHTAN O. NALPAK
EMULOV V. Preparation and Assay of Enzymes
Edited by YENDIS P. KCIWOLOC DNA NAHTAN 0. NALPAK
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by YENDIS P. KClWOLOC DNA NAHTAN 0. NALPAK
EMULOV VII. Cumulative Subject Index
Edited by YENDIS P. KCIWOLOC DNA NAHTAN 0. NALPAK
EMULOV VIII. Complex Carbohydrates
Edited by HTEBAZILE F. DLEFUEN DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by SILLIW A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited by DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV .1X Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by NITSUK HTENNEK
iivx
xviii
METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by HERBERT TABOR DNA CELIA WHITE TABOR
EMULOV XVIII. Vitamins and Coenzymes (Parts A, ,B dna )C
Edited yb DONALD B. McCORMICK DNA LEMUEL D. WRIGHT
EMULOV XIX. Proteolytic Enzymes
Edited yb GERTRUDE E. NNAMLREP DNA LORAND LASZLO
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KlVIE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb ANTHONY NAS PIETRO
EMULOV XXlV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA SAN PIETRO
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited yb C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited yb VICTOR GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by LAWRENCE NAMSSORG DNA KIVIE MOLDAVE
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part )F
Edited yb KIVIE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by FLEISCHER SIDNEY DNA LESTER PACKER
EMULOV XXXII. Biomembranes (Part B)
Edited by FLEISCHER SIDNEY DNA LESTER PACKER
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by MARTHA G. DENNIS DNA EDWARD a. DENNIS
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited yb WILLIAM B. JAKOBY DNA MEIR WILCHEK
EMULOV XXXV. Lipids (Part B)
Edited by JOHN M. NIETSNEWOL
METHODS NI ENZYMOLOGY xix
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by LEOJ G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XXXlX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by LEOJ G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by .W A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by NHOJ H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. JAKOBY DNA MEIR WILCHEK
EMULOV XLVII. Enzyme Structure (Part E)
Edited by .C H. .W HIRS DNA EGRES N. FEEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by .C H. W. HIRS DNA EGRES N. EFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by .C H. W. HIRS DNA EGRES .N FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by PATR1CIA A. HOFFEE DNA MARY ELLEN JONES
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
XX METHODS NI ENZYMOLOGY
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by SIDNEY REHCSIELF DNA LESTER REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by MARLENE A. DELucA
EMULOV LVIII. Cell Culture
Edited by WILLIAM B. JAKOBY DNA IRA PASTAN
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVlE MOLDAVE DNA LAWRENCE GROSSMAN
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by KlVlE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV 61. Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV 62. Vitamins and Coenzymes (Part D)
Edited by DONALD B. McCORMICK DNA LEMUEL D. WRIGHT
EMULOV 63. Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
Edited by DANIEL L. PURICH
EMULOV 64. Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and Com-
plex Enzyme Systems)
Edited by DANIEL L. PURICH
EMULOV 65. Nucleic Acids (Part I)
Edited by LAWRENCE GROSSMAN DNA KIVIE MOLDAVE
EMULOV 66. Vitamins and Coenzymes (Part E)
Edited by DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
EMULOV 67. Vitamins and Coenzymes (Part F)
Edited by DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
VOLUME 68. Recombinant DNA
Edited by RAY Wu
EMULOV 69. Photosynthesis and Nitrogen Fixation (Part C)
Edited by ANTHONY SAN PIETRO
EMULOV 70. Immunochemical Techniques (Part A)
Edited by HELEN VAN VUNAKIS DNA JOHN J. LANGONE
EMULOV 71. Lipids (Part C)
Edited by JOHN M. NIETSNEWOL
EMULOV 72. Lipids (Part D)
Edited by JOHN M. NIETSNEWOL