Current Research Topics in Applied Microbiology and Microbial Biotechnology: Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (Biomicroworld2007)

788 Pages·2009·40.3 MB·English

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Proceedings of the II International Conference on
Environmental, Industrial and Applied Microbiology
(BioMicroWorld2007)
edited by
Antonio Mendez-Vilas
Formatex Research Center, Spain
World Scientific
NEW JERSEY • LONDON • SINGAPORE • BEIJING • SHANGHAI • HONG KONG • TAIPEI • CHENNAI
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Published by
World Scientific Publishing Co. Pte. Ltd.
5 Toh Tuck Link, Singapore 596224
USA office: 27 Warren Street, Suite 401-402, Hackensack, NJ 07601
UK office: 57 Shelton Street, Covent Garden, London WC2H 9HE
Library of Congress Cataloging-in-Publication Data
International Conference on Environmental, Industrial, and Applied Microbiology
(2nd : 2007 : Seville, Spain)
Current research topics in applied microbiology and microbial biotechnology : proceedings of the II International Conference
on Environmental, Industrial, and Applied Microbiology (BioMicroWorld2007) / Antonio Méndez-Vilas (editor).
p. ; cm.
Includes bibliographical references.
ISBN-13: 978-981-283-754-7 (hardcover : alk. paper)
ISBN-10: 981-283-754-X (hardcover : alk. paper)
1. Microbiology--Congresses. 2. Biotechnology--Congresses. I. Méndez-Vilas, A. II. Title.
[DNLM: 1. Microbiology--Congresses. 2. Biotechnology--methods--Congresses. 3. Microbiological
Techniques--Congresses. QW 4 I596c 2009]
QR1.I527 2007
616.9'041--dc22
2008046073
British Library Cataloguing-in-Publication Data
A catalogue record for this book is available from the British Library.
Copyright © 2009 by World Scientific Publishing Co. Pte. Ltd.
All rights reserved. This book, or parts thereof, may not be reproduced in any form or by any means, electronic or
mechanical, including photocopying, recording or any information storage and retrieval system now known or to be
invented, without written permission from the Publisher.
For photocopying of material in this volume, please pay a copying fee through the Copyright Clearance Center, Inc.,
222 Rosewood Drive, Danvers, MA 01923, USA. In this case permission to photocopy is not required from the
publisher.
Printed in Singapore.
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Introduction
This book contains a compilation of papers presented at the II International Conference on
Environmental, Industrial and Applied Microbiology (BioMicroWorld2007) held in Seville (Spain)
on 28 November–1 December 2007, where over 550 researches of about 60 countries attended and
presented their cutting edge research.

The main goals of this meeting were to 1) identify new approaches and research opportunities in
applied microbiology presenting works which linked microbiology with research areas usually related
with other scientific and engineering disciplines, 2) consolidate international collaborative efforts and
3) communicate current research priorities and progress in the field. In other words, the focus of the
meeting was not only to present current science but also to discuss and synthesize research priorities
and directions for the future. The contents of this book mirror this focus.

There were many excellent presentations in the several conference sessions and plenary lectures.
The conference was structured in four main sessions covering the topics: 1) Environmental, Marine,
Aquatic Microbiology/ Geomicrobiology 2) Agriculture, Soil, Forest Microbiology 3) Food
Microbiology 4) Industrial Microbiology/ Future Bioindustries. And four additional special sessions
dedicated to: 1) Bioremediation 2) Microbial Production of Chemicals and Pharmaceuticals/
Biosurfactants/ Biotechnologically Relevant Enzymes and Proteins 3) Medical and Pharmaceutical
Microbiology 4) Techniques and Methods/ Microbiology Education.

The lectures presented by the three plenary speakers can be highlighted as good examples of modern
interdisciplinary applied microbiology: Dr. J.L. Ramos Martín, from the CSIC-Spanish National
Research Council (Granada, Spain), presented the lecture titled “The toluene paradox: to eat, to
tolerate or to run away”. Dr. M.L. González-Martín, from the Biosurfaces and Interfacial Phenomena
Group of the Department of Applied Physics, UEX (Badajoz, Spain), expounded on “Physico-
chemistry of microbial adhesion to materials: from macroscopic surface thermodynamics to modern
nano-scale technology”. Finally, Dr. Riitta Puupponen-Pimiä, from the VTT Technical Research
Centre of Finland (Espoo, Finland), presented the lecture titled: “Antimicrobial and antiadhesion
properties of bioactive berry compounds”

This book is made up of the works presented in all the above mentioned sessions. As a result of it, a
complete and heterogeneous book is presented here.

We would like to thank all members of the Organizing and Scientific Advisory Committees for the
work made during the conference preparation. We would also like to thank all the contributing
authors for sharing their knowledge and research, as well as all the reviewers for their advice, which
certainly helped to improve the quality, accuracy and relevance of the conference program and
publications. Lastly, we would like to thank EVONIK INDUSTRIES, BIOLOG, AES CHEMUNEX
and HORIZON SCIENTIFIC PRESS for sponsoring the conference.

We hope readers will find this book interesting and helpful to their research purposes and it helps to
consolidate the conference as a regular research meeting in the field.

Antonio Mendez-Vilas
BioMicroWorld2007 General Chairman

Formatex Research Center
Zurbaran 1, 2nd floor, 1st office
06002 Badajoz
Spain
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Contents
Introduction......................................................................................................................v
Agriculture, Soil, Forest Microbiology.........................................................................1
Anti-oxidative stress enzymes in Pleurotus ostreatus.............................................................3
Beauveria bassiana mutants display a different protein profile in comparison with
parental strain..........................................................................................................................8
Culturable microbial populations in a vineyard soil under different management
regimes: Influence on spontaneous must fermentation.........................................................12
Diagnosis and association of Olpidium bornovanus and MNSV with vine decline of
melon in Honduras................................................................................................................16
Effect of nitroaromatic compounds on the growth of potted plants......................................24
Effects of cattle grazing, trampling and excrement deposition on microbial nitrogen
transformations in upland soil...............................................................................................29
Evaluation of Crude Glycerol from Biodiesel Production as a plant pathogen control
agent......................................................................................................................................33
Extended nutrient limitation influences Ralstonia solanacearum survival in natural
water microcosms..................................................................................................................38
Genetic characterization of Plutella xylostella with resistance to formulates of
Bacillus thuringiensis at laboratory and field level...............................................................42
Hemicelluloses: from wood to the fermenter........................................................................46
Identification and characterization of a Cry7-like protein of Bacillus thuringiensis
GM-33 strain holotype for subsp. monterrey........................................................................51
Identification of actinomycetes with antifungal activity isolated from soil amended
with composts........................................................................................................................55
Identification of Mycobacterium sp. as Alfalfa endophytes using 16s rRNA gene
sequence analysis...................................................................................................................59
Identification of the respiratory chain of Armillaria mellea (A.m.) in mushroom state
and cultured in vitro...............................................................................................................64
Isolation and identification of sulphur-oxidizing bacteria from composted two-phase
olive mill waste amended with elemental sulphur.................................................................69
Manipulating the growth of bold and small grains in the ear of Triticum aestivum by
salicylhydroxamic acid..........................................................................................................74
New hosts for the enterobacterial phytopathogen Erwinia persicina....................................78
vii
Production and Evaluation of Polyclonal Antisera for Detection of Ralstonia
solanacearum........................................................................................................................83
Survival of Erwinia amylovora in rain water at low temperatures........................................88
The addition of copper sulphate to a non-selective medium improves the recovery of
plant associated bacteria: Erwinia amylovora as a model.....................................................92
The effects of various organic wastes applied into eroded soil on dehydrogenase
enzyme activity......................................................................................................................97
Use of vinasses in the control of fungi phytopathogens......................................................102
Utilization of organic and mineral amendments to control potato bacterial wilt disease....109
Viability of culturable soil microorganisms during freeze storage......................................114
Analytical and Imaging Techniques. Microscopy...................................................119
Characterization of Cell Walls from Mucoralean Fungi by Biochemical Composition,
Transmission Electron Microscopy and X-Ray Microanalysis...........................................121
Comparative analysis of different microbial techniques of quantification applied to
anaerobic digestion..............................................................................................................126
Comparison of different analytical processes for patulin determination in apple juice......131
Electroimmunoassay for detection of bacterial cells...........................................................136
Fine structure of wild type and mit- mutants of the yeast Saccharomyces cerevisiae.........140
Study of necessary pre-treatment for application of microbial techniques of
quantification to high content in solids samples..................................................................145
Environmental, Marine, Aquatic Microbiology. Geomicrobiology.......................151
A preliminar survey on the fungi of Doña Trinidad Cave, Ardales, Malaga, Spain...........153
A survey of microorganisms related to the biodeterioration of prehistoric paintings in
natural shelters from Aragon (Spain)..................................................................................157
An Investigation on Heterotrophic and Pathogenic Gastrointestinal Bacteria in
Otamiri River, Nigeria.........................................................................................................162
Assessment of the performance of porphyrin derivatives as photosensitizers for the
inactivation of bacterial endospores....................................................................................166
Biodegradation of aromatic contaminants present in industrial production waste
waters by Trichosporon cutaneum......................................................................................170
Biodegradation of exogenous DNA by bio-products used in domestic sewage
treatment..............................................................................................................................176
Biotechnological Potential of Phanerochaete chrysosporium UCP 963 and
Cunninghamella elegans UCP 596 in the Copper and Zinc Removal................................180
viii
Cadmium toxicity on Cunninghamella elegans: Ultrastructural damage and actin
cytoskeleton alterations.......................................................................................................184
Comparative analyses of microorganisms from different high-temperature volcanic
environments.......................................................................................................................188
Composition of halophilic bacteria survived in bioaerosol.................................................193
Control of Legionella pneumophila by disinfectants. Effects of sodium hypochlorite
against persistent strains......................................................................................................198
Degradation of phenols by Fusarium moniliforme..............................................................203
Degradative potential of marine bacterial isolates from the aquatic plant Posidonia
oceanica...............................................................................................................................208
Development of new primer systems for the detection of the polyphosphate kinase
gene in activated sludge.......................................................................................................213
Effect of Phenanthrene on the Germination, Radial Growth and Chitin and Chitosan
production by Cunninghamella elegans Lendner................................................................219
Effect of Pyrene on the growth of Rhodotorula sp..............................................................223
Effect of the introduction of an anaerobic phase on the protozoa community of an
SBR used for biodecolorization of an azo dye....................................................................228
Effect of water dilution and nutrient supplements (wood ash, urea and poultry
droppings) on biogas production from brewers spent grain................................................232
Evaluation of the impact of two aquaculture systems on bacterial communities of the
estuarine system Ria de Aveiro...........................................................................................236
Evaluation of the microbial diversity in anaerobic reactors fed with sucrose applied to
hydrogen production............................................................................................................240
Evidence of a bimodal effect on Saccharomyces cerevisiae UE-ME by vanadium (V)
3
stress – a dual response to different V O medium concentration detected in the rate
2 5
growth, GSH/GSSG, G6PD, CAT T and GR enzymatic activities.....................................245
Factorial design applied to biosurfactant production by Chromobacterium violaceum......249
Formation of Biofilms and Production of Enzymes by Bacillus subtilis on Surfaces of
Polyethylene Terephtalate Simulating Degradation............................................................254
Glutamylcysteine ligase gene of the ciliated protozoan Tetrahymena thermophila: A
potential tool for pollution monitoring................................................................................259
Identification of culturable psychrophilic yeasts isolated from sediments and melt
waters of the Calderone Glacier (Italy)...............................................................................264
In situ assessment of drinking water biostability using nascent reference biofilm ATR-
FTIR fingerprint..................................................................................................................268
Influence of light: dark cycle in the cellular composition of Nannochloropsis gaditana...273
ix