CURRENT FRONTIERS AND PERSPECTIVES IN CELL BIOLOGY Edited by Stevo Najman Current Frontiers and Perspectives in Cell Biology Edited by Stevo Najman Published by InTech Janeza Trdine 9, 51000 Rijeka, Croatia Copyright © 2012 InTech All chapters are Open Access distributed under the Creative Commons Attribution 3.0 license, which allows users to download, copy and build upon published articles even for commercial purposes, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech, authors have the right to republish it, in whole or part, in any publication of which they are the author, and to make other personal use of the work. Any republication, referencing or personal use of the work must explicitly identify the original source. As for readers, this license allows users to download, copy and build upon published chapters even for commercial purposes, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials, instructions, methods or ideas contained in the book. Publishing Process Manager Vedran Greblo Technical Editor Teodora Smiljanic Cover Designer InTech Design Team First published April, 2012 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from [email protected] Current Frontiers and Perspectives in Cell Biology, Edited by Stevo Najman p. cm. ISBN 978-953-51-0544-2 Contents Section 1 Cell Structures and Functions 1 Chapter 1 Tight Junctions 3 Lorenza González-Mariscal, Miguel Quirós, Mónica Díaz-Coránguez and Pablo Bautista Chapter 2 Tubulohelical Membrane Arrays, Annulate Lamellae and Nuclear Pores: Tripartite Membrane Architecture with the Participation of Nucleoporins 31 Siegfried Reipert and Elena Kiseleva Chapter 3 Actin Folding, Structure and Function: Is It a Globular or an Intrinsically Disordered Protein? 57 Olga I. Povarova, Anna I. Sulatskaya, Irina M. Kuznetsova and Konstantin K. Turoverov Chapter 4 Microtubules During the Cell Cycle of Higher Plant Cells 81 Elena Smirnova Section 2 Genetic Material: Structure and Expression 103 Chapter 5 Centromere Evolution: Digging into Mammalian Primary Constriction 105 Giuliana Giannuzzi, Claudia Rita Catacchio and Mario Ventura Chapter 6 mRNA Biogenesis in the Nucleus and Its Export to the Cytoplasm 131 Naoko Fujiwara, Takuya Shiki and Seiji Masuda VI Contents Chapter 7 Membrane Initiated Effects of 1α,25-Dihydroxyvitamin D in Prostate Cancer Cells: 3 Effects on AP1 and CREB Mediated Transcription 153 Dennis Larsson, Adele Jonas, Niklas Bergsten, Fredrik Ståhl and Sandra Karlsson Chapter 8 Genetic Transformation and Analysis of Protein-Protein Interaction of Class B MADS-Box Genes from Dendrobium moniliforme 163 Supatida Abdullakasim and Takashi Handa Section 3 Molecular and Cellular Regulatory Mechanisms 179 Chapter 9 Exploring Secrets of Nuclear Actin Involvement in the Regulation of Gene Transcription and Genome Organization 181 Yong Zhong Xu, Cynthia Kanagaratham and Danuta Radzioch Chapter 10 Signaling of Receptor Tyrosine Kinases in the Nucleus 211 Sally-Anne Stephenson, Inga Mertens-Walker and Adrian Herington Chapter 11 G Protein-Coupled Receptors-Induced Activation of Extracellular Signal-Regulated Protein Kinase (ERK) and Sodium-Proton Exchanger Type 1 (NHE1) 235 Maria N. Garnovskaya Chapter 12 The Kinetochore and Mitosis: Focus on the Regulation and Correction Mechanisms of Chromosome-to-Microtubule Attachments 259 Rita M. Reis and Hassan Bousbaa Chapter 13 Molecular and Sub-Cellular Gametogenic Machinery of Stem and Germline Cells Across Metazoa 279 Andrey I. Shukalyuk and Valeria V. Isaeva Chapter 14 Drosophila: A Model System That Allows in vivo Manipulation and Study of Epithelial Cell Polarity 315 Andrea Leibfried and Yohanns Bellaïche Chapter 15 Development and Cell Polarity of the C. elegans Intestine 335 Olaf Bossinger and Michael Hoffmann Chapter 16 Intercellular Communication 361 Nuri Faruk Aykan Contents VII Section 4 Cellular Basis of Disease and Therapy 377 Chapter 17 Adult Stem Cells in Tissue Homeostasis and Disease 379 Elena Lazzeri, Anna Peired, Lara Ballerini and Laura Lasagni Chapter 18 Claudins in Normal and Lung Cancer State 423 V. Morales-Tlalpan, C. Saldaña, P. García-Solís, H. L. Hernández-Montiel and H. Barajas-Medina Chapter 19 Biology of Cilia and Ciliopathies 423 David Alejandro Silva, Elizabeth Richey and Hongmin Qin Chapter 20 The Roles of ESCRT Proteins in Healthy Cells and in Disease 453 Jasmina Ilievska, Naomi E. Bishop, Sarah J. Annesley and Paul R. Fisher Chapter 21 Autologous Grafts of Mesenchymal Stem Cells – Between Dream and Reality 481 Frédéric Torossian, Aurelie Bisson, Laurent Drouot, Olivier Boyer and Marek Lamacz Section 5 New Methods in Cell Biology 501 Chapter 22 Salivary Glands: A Powerful Experimental System to Study Cell Biology in Live Animals by Intravital Microscopy 503 Monika Sramkova, Natalie Porat-Shliom, Andrius Masedunkas, Timothy Wigand, Panomwat Amornphimoltham and Roberto Weigert Chapter 23 Evaluation of Mitochondrial DNA Dynamics Using Fluorescence Correlation Analysis 525 Yasutomo Nomura Chapter 24 Regeneration and Recycling of Supports for Biological Macromolecules Purification 535 Marcello Tagliavia and Aldo Nicosia Section 1 Cell Structures and Functions