Description:were transferred to liquid Murashige and Skoog (MS) medium containing with 2 mg/L IBA, 0.5 mg/L . days of preculture with 0.3 and 0.5 M sucrose. Moreover,. Baghdadi et al. (2010) reported that maximum survival. (100%) rates were obtained after preculture of crocus calli on 0.1 and 0.5 M sucrose