Corneal Regeneration: Methods and Protocols

253 Pages·2020·7.738 MB·English

by  Mark Ahearne

Methods in
Molecular Biology 2145
Mark Ahearne Editor
Corneal
Regeneration
Methods and Protocols
M M B
ETHODS IN OLECULAR IO LO GY
SeriesEditor
JohnM.Walker
School of Lifeand MedicalSciences
University ofHertfordshire
Hatfield, Hertfordshire, UK
Forfurther volumes:
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For over 35 years, biological scientists have come to rely on the research protocols and
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Corneal Regeneration
Methods and Protocols
Edited by
Mark Ahearne
Trinity Centre for Biomedical Engineering, Trinity College Dublin, Dublin, Ireland
Editor
MarkAhearne
TrinityCentreforBiomedical
Engineering
TrinityCollegeDublin
Dublin,Ireland
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-0716-0598-1 ISBN978-1-0716-0599-8 (eBook)
https://doi.org/10.1007/978-1-0716-0599-8
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Preface
Cornealblindnessisoneofthemostcommoncausesofblindnessworldwide.Totreatthis
condition,therehasbeenmuchinterestintryingtodevelopnewtherapiestoregenerateor
repair damaged and diseased corneal tissue. This book will provide a detailed overview of
several laboratory techniques that are used to develop regenerative therapies to help treat
corneal blindness. These include how to optimize cell culture conditions, how to apply
gene-editingtechniques,howtopreparedifferenttypesofscaffoldforcornealregeneration,
andhowtoevaluatethesuccessofthesetherapiesusinginvitroandinvivomodelsandcell
and material characterization techniques. This book will be of interest to new and experi-
encedlaboratoryresearchersworkingondifferentaspectsofcornealregenerationaswellas
ophthalmologists and patients interested in learning more about the latest techniques and
technology.
Dublin,Ireland MarkAhearne
v
Contents
Preface ..................................................................... v
Contributors................................................................. ix
1 IsolationandCultureofCornealStromalStemCells........................ 1
RichardM.Nagymihaly,MortenC.Moe,andGoranPetrovski
2 InVitroExpansionofCornealEndothelialCellsforTransplantation.......... 17
KimSanterre,IsabelleXu,MathieuThe´riault,andSte´phanieProulx
3 PrimaryCultureofCornea-LimbalEpithelialCellsInVitro.................. 29
FinbarrO’Sullivan
4 OptimizationofHumanLimbalStemCellCulturebyReplating
aSingleLimbalExplant.................................................. 39
MarinaL(cid:1)opez-Paniagua,TeresaNieto-Miguel,SaraGalindo,
LauraGarcı´a-Posadas,AnadelaMata,RosaM.Corrales,
MargaritaCalonge,andYolandaDiebold
5 AGuidetotheDevelopmentofHumanCorneaOrganoids
fromInducedPluripotentStemCellsinCulture............................ 51
JamesW.Foster,KarlJ.Wahlin,andShuktiChakravarti
6 GeneEditingforCornealStromalRegeneration............................ 59
TaraMoore,ConnieChao-Shern,LarryDeDionisio,
KathleenA.Christie,andM.AndrewNesbit
7 PreparationandAdministrationofAdeno-associatedVirusVectors
forCornealGeneDelivery ............................................... 77
LiujiangSong,JacquelynJ.Bower,andMatthewL.Hirsch
8 TheSelf-assemblyApproachasaToolfor theTissueEngineering
ofaBi-lamellarHumanCornea........................................... 103
Ga¨etanLe-Bel,PascaleDesjardins,CamilleCouture,
LucieGermain,andSylvainL.Gue´rin
9 FormationofCornealStromal-LikeAssembliesUsingHumanCorneal
FibroblastsandMacromolecularCrowding ................................ 119
MehmetGu¨rdal,Gu¨linnazErcan,andDimitriosI.Zeugolis
10 PreparationofDriedAmnioticMembraneforCornealRepair................ 143
AndrewHopkinson,EmilyR.Britchford,andLauraE.Sidney
11 FabricationofCornealExtracellularMatrix-DerivedHydrogels .............. 159
MarkAhearneandJuliaFerna´ndez-Pe´rez
12 SynthesisandApplicationofCollagensforAssemblingaCornealImplant ..... 169
ElleEdin,FionaSimpson,andMayGriffith
13 DevelopmentandValidationofa3DInVitroModeltoStudy
theChemotacticBehaviorofCornealStromalFibroblasts ................... 185
EvrimCerenKabak,JuliaFerna´ndez-Pe´rez,andMarkAhearne
vii
viii Contents
14 FemtosecondLaser-AssistedSurgeryforImplantation
ofBioengineeredCornealStromatoPromoteCornealRegeneration.......... 197
NeilLagaliandMehrdadRafat
15 TheUseofAnimalModelstoAssessEngineeredCornealTissue ............. 215
RobertThomasBradyandPeterW.Madden
16 X-RayDiffractionImagingofCornealUltrastructure ....................... 231
KeithM.Meek,AndrewJ.Quantock,SallyHayes,andJamesBell
Index ...................................................................... 249
Contributors
MARKAHEARNE • DepartmentofMechanicalandManufacturingEngineering,Schoolof
Engineering,TrinityCollegeDublin,TheUniversityofDublin,Dublin,Ireland;Trinity
CentreforBiomedicalEngineering,TrinityBiomedicalSciencesInstitute,TrinityCollege
Dublin,TheUniversityofDublin,Dublin,Ireland
JAMESBELL • StructuralBiophysicsGroup,SchoolofOptometryandVisionSciences,Cardiff
University,Cardiff,UK
JACQUELYNJ.BOWER • LinebergerComprehensiveCancerCenter,UniversityofNorth
CarolinaatChapelHill,ChapelHill,NC,USA
ROBERTTHOMASBRADY • DepartmentofOphthalmology,MaterMisericordiaeUniversity
Hospital,Dublin,Ireland
EMILYR.BRITCHFORD • AcademicOphthalmology,DivisionofClinicalNeuroscience,School
ofMedicine,UniversityofNottingham,Nottingham,UK;NuVisionBiotherapiesLtd,
MediCity,Nottingham,UK
MARGARITACALONGE • GrupodeSuperficieOcular,InstitutoUniversitariode
Oftalmobiologı´aAplicada(IOBA),UniversidaddeValladolid,Valladolid,Spain;Centro
deInvestigaci(cid:1)onBiome´dicaenReddeBioingenierı´a,BiomaterialesyNanomedicina
(CIBER-BBN).InstitutodeSaludCarlosIII,Madrid,Spain
SHUKTICHAKRAVARTI • DepartmentofOphthalmologyandPathology,NYULangone
Health,AlexandriaLifeSciencesCenter,NewYork,NY,USA
CONNIECHAO-SHERN • BiomedicalSciencesResearchInstitute,UlsterUniversity,Coleraine,
NorthernIreland,UK;AvellinoLabUSA,Inc.,MenloPark,CA,USA
KATHLEENA.CHRISTIE • BiomedicalSciencesResearchInstitute,UlsterUniversity,
Coleraine,NorthernIreland,UK
ROSAM.CORRALES • GrupodeSuperficieOcular,InstitutoUniversitariodeOftalmobiologı´a
Aplicada(IOBA),UniversidaddeValladolid,Valladolid,Spain;CentrodeInvestigaci(cid:1)on
Biome´dicaenReddeBioingenierı´a,BiomaterialesyNanomedicina(CIBER-BBN).
InstitutodeSaludCarlosIII,Madrid,Spain
CAMILLECOUTURE • Centreuniversitaired’ophtalmologie-recherche(CUO-Recherche)et,
Universite´Laval,Que´bec,QC,Canada;Centrederechercheenorganoge´ne`seexpe´rimentale
del’Universite´Laval/LOEX,CentrederechercheduCHUdeQue´bec-Universite´Laval,
Universite´Laval,Que´bec,QC,Canada;De´partementdechirurgie,Faculte´deme´decine,
Universite´Laval,Que´bec,QC,Canada;De´partementd’ophtalmologie,Faculte´deMe´
decine,Universite´Laval,Que´bec,QC,Canada
LARRYDEDIONISIO • AvellinoLabUSA,Inc.,MenloPark,CA,USA
PASCALEDESJARDINS • Centreuniversitaired’ophtalmologie-recherche(CUO-Recherche)et,
Universite´Laval,Que´bec,QC,Canada;Centrederechercheenorganoge´ne`seexpe´rimentale
del’Universite´Laval/LOEX,CentrederechercheduCHUdeQue´bec-Universite´Laval,
Universite´Laval,QCQue´bec,Canada;De´partementd’ophtalmologie,Faculte´deMe´decine,
Universite´Laval,Que´bec,QC,Canada
YOLANDADIEBOLD • GrupodeSuperficieOcular,InstitutoUniversitariodeOftalmobiologı´a
Aplicada(IOBA),UniversidaddeValladolid,Valladolid,Spain;CentrodeInvestigaci(cid:1)on
Biome´dicaenReddeBioingenierı´a,BiomaterialesyNanomedicina(CIBER-BBN).
InstitutodeSaludCarlosIII,Madrid,Spain
ix
x Contributors
ELLEEDIN • Maisonneuve-RosemontHospitalResearchCentre,Montre´al,QC,Canada;
DepartmentofOphthalmologyandInstituteofBiomedicalEngineering,Universite´de
Montre´al,Montre´al,QC,Canada
GU¨LINNAZERCAN • FacultyofMedicine,DepartmentofMedicalBiochemistry,Ege
University,Izmir,Turkey;DepartmentofStemCell,InstituteofHealthSciences,Ege
University,Izmir,Turkey
JULIAFERNA´NDEZ-PE´REZ • DepartmentofMechanicalandManufacturingEngineering,
SchoolofEngineering,TrinityCollegeDublin,TheUniversityofDublin,Dublin,Ireland;
TrinityCentreforBiomedicalEngineering,TrinityBiomedicalSciencesInstitute,Trinity
CollegeDublin,TheUniversityofDublin,Dublin,Ireland
JAMESW.FOSTER • TheWilmerEyeInstitute,JohnsHopkinsUniversity,Baltimore,MD,
USA
SARAGALINDO • GrupodeSuperficieOcular,InstitutoUniversitariodeOftalmobiologı´a
Aplicada(IOBA),UniversidaddeValladolid,Valladolid,Spain;CentrodeInvestigaci(cid:1)on
Biome´dicaenReddeBioingenierı´a,BiomaterialesyNanomedicina(CIBER-BBN).
InstitutodeSaludCarlosIII,Madrid,Spain
LAURA GARCI´A-POSADAS • GrupodeSuperficieOcular,InstitutoUniversitariode
Oftalmobiologı´aAplicada(IOBA),UniversidaddeValladolid,Valladolid,Spain
LUCIE GERMAIN • Centreuniversitaired’ophtalmologie-recherche(CUO-Recherche)et,
Universite´Laval,Que´bec,QC,Canada;Centrederechercheenorganoge´ne`seexpe´rimentale
del’Universite´Laval/LOEX,CentrederechercheduCHUdeQue´bec-Universite´Laval,
Universite´Laval,QCQue´bec,Canada;De´partementdechirurgie,Faculte´deme´decine,
Universite´Laval,Que´bec,QC,Canada;De´partementd’ophtalmologie,Faculte´deMe´
decine,Universite´Laval,Que´bec,QC,Canada
MAYGRIFFITH • Maisonneuve-RosemontHospitalResearchCentre,Montre´al,QC,Canada;
DepartmentofOphthalmologyandInstituteofBiomedicalEngineering,Universite´de
Montre´al,Montre´al,QC,Canada
SYLVAIN L.GUE´RIN • Centreuniversitaired’ophtalmologie-recherche(CUO-Recherche)et,
Universite´Laval,Que´bec,QC,Canada;Centrederechercheenorganoge´ne`seexpe´rimentale
del’Universite´Laval/LOEX,CentrederechercheduCHUdeQue´bec-Universite´Laval,
Universite´Laval,Que´bec,QC,Canada;De´partementd’ophtalmologie,Faculte´deMe´
decine,Universite´Laval,Que´bec,QC,Canada
MEHMETGU¨RDAL • Regenerative,Modular&DevelopmentalEngineeringLaboratory
(REMODEL),NationalUniversityofIrelandGalway(NUIGalway),Galway,Ireland;
ScienceFoundationIreland(SFI)CentreforResearchinMedicalDevices(CU´RAM),
NationalUniversityofIrelandGalway(NUIGalway),Galway,Ireland;Facultyof
Medicine,DepartmentofMedicalBiochemistry,EgeUniversity,Izmir,Turkey
SALLYHAYES • StructuralBiophysicsGroup,SchoolofOptometryandVisionSciences,Cardiff
University,Cardiff,UK
MATTHEWL.HIRSCH • GeneTherapyCenter,UniversityofNorthCarolinaatChapelHill,
ChapelHill,NC,USA;DepartmentofOphthalmology,UniversityofNorthCarolina,
ChapelHill,NC,USA
ANDREWHOPKINSON • AcademicOphthalmology,DivisionofClinicalNeuroscience,Schoolof
Medicine,UniversityofNottingham,Nottingham,UK;NuVisionBiotherapiesLtd,
MediCity,Nottingham,UK
EVRIM CEREN KABAK • TrinityCentreforBiomedicalEngineering,TrinityBiomedical
SciencesInstitute,TrinityCollegeDublin,TheUniversityofDublin,Dublin,Ireland;
BiotechnologyUnit,NobelPharmaceuticalsA.S,Gebze,Kocaeli,Turkey