J. Res. ANGRAU Vol. XLI No.3 pp 1-144, July-Sep., 2013 1 The Journal of Research ANGRAU (Published quarterly in March, June, September and December) ADVISORY BOARD Dr. A. Siva Sankar Dr. R. Ranga Reddy Director of Extension, Director of Research, Rajendranagar, Hyderabad. Rajendranagar, Hyderabad. Dr. P. Chandrasekhar Rao Dr. T.V. Satyanarayana Prof. & University Head, Dean of Agril. Engineering & Technology & Dept. of Soil Science & Agril. Chemistry Dean of Homescience i/c Rajendranagar, Hyderabad. Rajendranagar, Hyderabad. Dr. K. Veeranjaneyulu University Librarian ANGRAU, Rajendranagar, Hyderabad. EDITORIAL COMMITTEE MEMBERS Dr. T. Pradeep Dr. A. Mani Principal Scientist(Breeding), Associate Professor Maize Research Station, Dept. of Agril. Engineering & Technology ARI Campus, Rajendranagar, College of Agriculture, Rajendranagar, Hyderabad. Hyderabad. Dr. R. Sudhakar Dr. T. Ramesh Principal Scientist (Plant Pathology), Professor Seed Research & Technology Centre, Dept. of Plant Physiology ANGRAU, Rajendranagar, Hyderabad. College of Agriculture, Rajendranagar, Hyderabad. Dr. M. Venkata Ramana Principal Scientist (Agron) & Head, Dr. I. Sreenivas Rao AICRP on Integrated Farming Systems, Professor and Head, Diamond Jubilee Block,College of Agriculture, Dept. of Extension Education, College of Agriculture, ANGRAU, Rajendranagar, Hyderabad. ANGRAU, Rajendranagar, Hyderabad. Dr. G. Sravan Kumar Dr. T. Neeraja Additional Controller of Examinations & Professor, Dept. of Resource Management and University Head, Department of English, Consumer Sciences, College of Agriculture, Rajendranagar, College of Home Science, Hyderabad. Saifabad, Hyderabad. EDITOR MANAGING EDITOR Dr. P. Chandrasekhar Rao Dr. K. Anand Singh Professor & University Head, Principal Agricultural Information Officer Department of SSAC, College of Agriculture, AI&CC and ANGRAU Press, Rajendranagar, ANGRAU, Rajendranagar, Hyderabad. Hyderabad. RESEARCH EDITOR Dr. A. Lalitha AI&CC and ANGRAU Press, Rajendranagar, Hyderabad with effect from April, 2012: SUBSCRIPTION TARIFF Individual (Annual) : Rs. 300/- Institutional (Annual) : Rs. 1200/- Individual (Life) : Rs. 1200/- Printing Charges : Rs. 100/- per page DDs may be sent to The Managing Editor, Journal of Research ANGRAU, Agricultural Information & Communication Centre and ANGRAU Press - Agricultural Research Institute, Rajendranagar - Hyderabad - 500 030 2 CONTENTS PART I : PLANT SCIENCE Effect of polypropylene packaging on shelf life and quality of custard apple Annona squamosa L. cv. Balanagar fruits stored at 15±1oC A. Venkatram, A. Bhagwan and M. Pratap 1 Seasonal Incidence of Iinseed insect pests and their natural enemies Harshwardhan Gupta and B. P. Katlam 7 Components of genetic variation for macrophomina phaseolona resistance in maize K.Murali Krishna, G.K Chikkappa and G. Manjulatha 12 Summer season survey for incidence of Maruca vitrata (g.) (pyralidae: lepidoptera) and its natural enemies on greengram and other alternative hosts in main pulse growing tracts of Khammam District, Andhra pradesh Ch. Sandhya Rani , G. Ramachandra Rao, MSV Chalam, Patibanda Anil Kumar and V Srinivasa Rao 16 Nature of Gene action in new cms and restorer lines of rice (oryza sativa l.) Zakir Hussain and Narendra Kulkarni 21 Evaluation of changes in soil quality under different land use environs of chanvelly village, Rangareddy District of Andhra Pradesh- a case study M. Ram Prasad and V.Govardhan 25 Genetic variability, correlation and path coefficient analysis for yield and yield components in mungbean Vigna radiata l. Wilczek M. Jyothsna and and CH. Anuradha 31 Effect of post harvest application of antioxidants on shelf life and quality of custard apple annona squamosa L. cv. Balanagar fruits stored at 15±1oC A. Venkatram, A. Bhagwan and M. Pratap 40 Production potential and economic viability of food – forage based cropping systems in Southern Telangana Region of Andhra Pradesh V.Chandrika, R. Balaji Naik, K.B. Suneetha Devi and M. Shanti 47 PART II : SOCIAL SCIENCE Attitude of farmers and extension officers towards Agricultural Technology Management Agency (ATMA) & suggestions for effective functioning M. Rama Devy, M. Surya Mani and N. Shandhya Shenoy 53 Knowledge and extent of adoption of improved practices of chickpea through KVK interventions P.Ganesh Kumar, M.K. Jyosthna and P.Lakshmi Reddy 58 A study on purpose of using ICT tools among farming community K. P. Raghuprasad, S.C. Devaraja and Y.M Gopala 64 Problems faced by women entrepreneurs and suggestions for production & marketing of Products G.Padmini Devi, P. Rameshkumar Reddy and Ch. Venugopal Reddy 70 A study on growth, performance and impact of Kisan Credit Cards on farmer’s income in Rajasthan – An Economic Approach S. S. Meena and Dr. G. P. Reddy 75 3 PART III : HOME SCIENCE Utility of herbal products in antimicrobial finishing of cotton fabrics D.Anitha, S.Dhana Laxmi and D.Mahesh 82 Impact of personal variables on work related musculoskeletal symptoms among women working in packing units of pharmaceutical industry V. Prasuna, T. Neeraja, P. Radha Rani and A. Sharada devi 88 PART IV : RESEARCH NOTE Cooling effect of body on milk production and feed consumption in the cross bred cows Suresh Rathod, M.Kishan Kumar and Ch.Sreedhar 94 PART V : RESEARCH NOTE Farmers’ suggestions for better participatory management of tank irrigation system Kadiri Mohan1 and P.Rameshkumar Reddy 97 Character association and path coefficient analysis for seed yield in quality protein maize (zea mays L.) K. Vijay Kumar, M. R. Sudarshan, Kuldeep Singh Dangi and S. Madhusudan Reddy 100 Effect of gamma radiation on germination parameters in promising rice cultivars during long term storage L.Vidya Kumari, M. Sreedhar, S.Vanisree and K. Keshavulu 105 Influence of Nutritional Information on food labels on consumer buying Swetha Kodali, Neeraja Telaprolu and P. Radha Rani 110 Variability in yield and its component traits in quality protein maize genotypes (zea mays l.) K. Vijay Kumar, M. R. Sudarshan, Kuldeep Singh Dangi and S. Madhusudan Reddy 113 Correlation and path analysis in greengram (Vigna radiata (L.) Wilczek) Srikanth Thippani, K. B. Eswari and M. V. Brahmeswar Rao 120 Influence of row spacing and phosphorus levels on seed production of dhaincha (Sesbania aculeata) B. Venkanna, B. Joseph, K.B. Suneetha Devi and A. Siva Shankar 124 Scale construction for measuring the attitude towards pursuing self employment in agriculture Kadiri Mohan1 and P.Ramesh Kumar Reddy 127 Response of safflower (carthamus tinctorius l.) genotypes to plant density on yield components, yield and economics under rainfed conditions P. Satish, C. Sudhakar and C. Sudharani 130 Sensory properties of guar-gum incorporated tomato sauce E. Sharath and K. Uma Maheswari 132 4 J.Res. ANGRAU 41(3) 1-6, 2013 EFFECT OF POLYPROPYLENE PACKAGING ON SHELF LIFE AND QUALITY OF CUSTARD APPLE Annona squamosa L. cv. BALANAGAR FRUITS STORED AT 15±1oC A. VENKATRAM, A. BHAGWAN and M. PRATAP Fruit Research Station, Sangareddy, Dr. Y.S.R. Horticultural University, Medak (District) – 502 110 Date of Receipt : 05-06-2013 Date of Acceptance : 24.08.13 ABSTRACT A study was conducted at fruit research station, Sangareddy, Dr. Y.S.R. Horticultural University, Medak (district) during 2009-11 to know the effect of polypropylene package (fruits packed in polypropylene bags with 3% O + 5% CO or 3% O + 10% CO or 5% O + 5% CO or 5% O + 10% CO or air) on shelf life and quality of custard 2 2 2 2 2 2 2 2 apple cv. Balanagar fruits stored at 175±1oC. The study established the fact that various physico-chemical parameters viz., days taken for ripening, shelf life, total soluble solids, acidity, brix-acid ratio, sugars (reducing, total and non- reducing) and ascorbic acid of the fruit studied at an interval of 2 days during storage were significantly improved by polypropylene bag flushed with different concentration of O + CO gasses (modified atmosphere package). 2 2 Comparatively higher shelf life of fruits i.e. 12.28 days with fruits packed in polypropylene bags flushed with air. Custard apple Annona squamosa L. belongs life and conserve the quality of packed produce to family annonaceae is an arid zone fruit grown in (Deepak Raj Rai et al., 2002). Active MA can be done India. Out of 100 species of Annona only custard by pulling a slight vacuum and replacing the package apple, cherimoya, soursop, bullock’s heart and atmosphere with the desirable gas mixture (Kader et atemoya are of major commercial importance. Of al., 1986). Low O and or high CO gas mixtures in 2 2 these, custard apple (synonyms: Sithaphal, Sharifa, the package atmosphere has been shown to reduce Sugar apple, Sweetsop) is the most popular and the ethylene production rates, retard softening of widely used dessert fruit having the maximum tissue and slow down various compositional changes production efficiency. The plants are hardy, drought associated with ripening resulting in reduced resistant and can thrive well on marginal and respiration there by delay the fruit ripening process neglected soils with minimum inputs (Rajput, 1985). of many tropical and subtropical fruits (Ranganna et Custard apple is the delicious table fruit and is valued al., 2009). Hence, the present investigation was mainly for its sweet, mild flavored pulp. The fruit is carried out to study the effect of polypropylene botanically referred as syncarpium and aggregate of packaging for enhancing shelf life and quality of fruitlets, each pocessing edible fleshy mass of soft, custard apple cv. Balanagar fruits stored at 15±1oC. juicy and granular textured pulp. It is a climacteric MATERIALS AND METHODS fruit, ripening starts soon after detached from the tree (Wills et al., 2001). It is highly perishable fruit with The experiment was carried out during the short shelf life of 1-2 days after ripening. The lack of year 2009-11 at Fruit Research Station, Sangareddy, information on the post harvest handling of this highly Dr. Y.S.R. Horticultural University, Medak district. perishable fruit has resulted in huge losses. Custard apple cv. Balanagar fruits were harvested Extension of storage life in custard apple even for a when fruit is in light green colour, yellowish white day or two will go a long way in increasing the shelf colour between the carpels and initiation of cracking life, making it much easier to handle the fruit. Vishnu of the skin between the carpels. Matured fruits of Prasanna et al. (2000) reported that the safe range uniform size, firm, free from disease and injuries were of storage temperature of custard apple was found directly picked from orchard and brought to the to be 15-20 oC, with maximum shelf life at 15 oC. laboratory. The fruits were cleaned with running tap water to remove the adherent dirt material and then Modified atmosphere package (MAP) is intended to allowed to dry in shade. The fruits were surface create an appropriate gaseous atmosphere around a disinfected with 0.1% (w/v) carbendazim solution for commodity packed in film packages to enhance shelf 2 minutes. The fruits were then packed in email: [email protected] 5 VENKATRAM et al polypropylene bags (PPB) with the following amount the days taken for ripening of custard apple fruits of CO and O gases flushed with MAP machine: T- (Kamble and Chavan, 2005). Similar results on using 2 2 1 PPB with 3% O + 5% CO T - PPB with 3% O + modified atmosphere package as also reported by 2 2, 2 2 10% CO , T - PPB with 5% O + 5% CO , T - PPB Yonemoto et al. (2002) in cherimoya and Bolivar- 2 3 2 2 4 with 5% O + 10% CO , T - PPB with Air, T - Control Fernandez et al. (2009) in custard apple fruits. 2 2 5 6 (no packing). The packed fruits were then stored at Shelf life (days) 15±1oC temperature in horizontal racks. The physico- chemical parameters like days taken for ripening, The shelf life (Table 1) of custard apple fruits shelf life, TSS, acidity, brix-acid ratio, sugars differed significantly among different treatments with (reducing and total) and ascorbic acid were estimated maximum shelf life of 12.28 days (44.47% increase at an interval of 2 days during storage. over control) recorded in fruits packed in PPB with air, which was on par with PPB flushed with 3% O + The stage wherein more than 50% of the 2 5% CO and minimum in control (8.50 days). The stored fruits became ripen was considered as end of 2 extended shelf life of fruits is due to reduction in days taken for ripening. The stage wherein more than ethylene production attributed to modified atmosphere 50% of the stored fruits became unfit for consumption created by accumulation of CO and depletion of O was considered as end of shelf life and expressed 2 2 and maintenance of high humidity inside the as mean number of days. The total soluble solids polypropylene bags (Magdaline et al., 2001). These (TSS, oBrix) and titrable acidity (%) were determined results are in conformation with the findings of Vishnu by using ERMA hand refractometer and titration Prasanna et al. (2000) in custard apple. method, respectively (Ranganna, 1986). Brix-Acid ratio was calculated by dividing the TSS value by TSS (oBrix) the acid value. The sugars (reducing and total %) Significant differences were observed in TSS present in the custard apple pulp were determined (Table 2) for the stored fruits. At initial stage of by the method of Lane and Eyon. The non-reducing storage TSS was minimal (18.50oBrix), which sugars (%) were determined by subtracting reducing increased gradually with advancement in storage. sugars from total sugars. Ascorbic acid content (mg/ Highest TSS content was documented at 12 days 100 g of pulp) was determined by 2, 6-dichlorophenol after storage (23.61oBrix). In packaging treatments, indophenol titration method (Ranganna, 1986). The the annona fruits packed in PPB flushed with different experiment was conducted in completely randomized concentration of O and CO extended their influence 2 2 design with factorial concept and each treatment in recording lower TSS than the control. The replicated thrice. interaction effect between the treatments and storage RESULTS AND DISCUSSION period were also significant, which showed a steep increase in the TSS with progressive ripening of the Days taken for ripening fruits, as a fact the soluble solids rise concomitantly Days taken for ripening (Table 1) among with the respiratory increase in annonaceous fruits different treatments differed significantly, days taken and reach a maximum after the onset of second for ripening were maximum (10.43 days) with fruits respiratory rise (Martinez et al., 1993). Similar result packed in PPB flushed with 5% O + 10% CO or 5% also observed by Alique (1995) in cherimoya and 2 2 O + 5% CO or 3% O + 10% CO and minimum in Chunprasert et al. (2006) ‘Neang’ sugar apple fruits. 2 2 2 2 control (7.60 days). The days taken for ripening of Titrable acidity (%) fruits packed in PPB with 3% O + 5% CO were at 2 2 par with PPB with air. Lowering of storage The titrable acidity of stored custard apple temperature from ambient (27ÚC) to 15 oC and fruits subjected to different treatments is presented treatment of fruits to fungicide or waxol and modified in table 2. At initial stage of storage, acidity was atmosphere package is may be due to reduction in minimal (0.16%), which increased gradually with ethylene synthesis thereby lowered the rate of advancement in storage. Highest acidity content was deteriorative physiological, biological and biochemical documented at 12 days after storage (0.44%). With activities in ripening thereby resulted in extending regard to packaging treatments, the annona fruits 6 EFFECT OF POLYPROPYLENE PACKAGING ON SHELF LIFE packed in PPB flushed with different concentration sugars content of the fruit was highest at 12 days of O and CO extended their improvement in after storage (18.43%). With regard to packaging 2 2 recording higher acidity than the control. The treatments, the annona fruits packed in PPB flushed interaction effect between the treatments and storage with different concentration of O and CO exhibited 2 2 period were not significant. The increase in acidity their effect in recording lower total sugars than the can be ascribed to the production of organic acids control. At initial stage of storage, non-reducing during ripening as reported by Gutierrez et al. (1994). sugars was minimal (1.25%), which increased gradually with advancement in storage. Highest non- These results are in conformation with the findings reducing sugars was documented at 12 days after of Paull et al. (1983) in cherimoya fruits. storage (1.60%). With regard to packaging treatments, Brix-acid ratio the annona fruits packed in PPB flushed with different Significant differences were observed in brix- concentration of O and CO extended their 2 2 improvement in recording lower non-reducing sugars acid ratio (Table 2) for the stored fruits. At initial stage than the control. of storage brix-acid ratio was maximal (115.60), which decreased gradually with advancement in storage. The sugars increased gradually with Lowest brix-acid ratio was documented at 10 days advancement in storage due to the starch declined after storage (53.55). With regard to packaging drastically and there was also a coincidence in the treatments, the annona fruits packed in PPB flushed increase of sugars and the decline of starch content, with different concentration of O and CO extended indicating a hydrolysis of starch into its constituent 2 2 their improvement in recording lower brix-acid ratio sugars during fruit ripening and the onset of ethylene than the control. The interaction effects between the emission coincided with an increase in the respiration treatments and storage period were also significant, (Martinez et al., 1993). Lowest sugars was which showed a steep increase in the brix-acid ratio documented in annona fruits packed in PPB flushed with progressive ripening of the fruits. Though both with different concentration of O and CO is may be 2 2 due to slow ripening process in the modified TSS and acidity had shown initial increase followed atmosphere packaging which leads to slow build up by decrease, but an increase in acidity is very less, of the sugars as reported by Johnson and Ertorn therefore, the brix-acid ratio increased initially and (1983). These results are in conformation with the decreased later indicating that the magnitude of findings of Alique (1995) and Sanchez et al. (1998) increase or decrease in TSS is more as compared to in cherimoya fruits. acidity during storage. The fruits packed in PPB flushed with different concentration of O and CO Ascorbic acid (mg/100 g) 2 2 recorded lower brix-acid ratio and it may be due to Significant differences were observed in lower TSS and higher acidity (Kramchote et al., 2008). ascorbic acid (Table 3) for the stored fruits. At initial stage of storage, ascorbic acid content was minimal Sugars (%) (41.30 mg/100 g) and maximum at 6 days after storage Significant differences were observed in (49.39 mg/100 g). The ascorbic acid of annona sugars (reducing, total and non-reducing) (Table 3) increased from 0 to 6th day and then declined by 31.48 for the stored fruits for days, treatments and their mg/100g on 12 days after storage. With regard to interactions. At initial stage of storage reducing packaging treatments, the annona fruits packed in sugars was minimal (11.90%), which increased PPB flushed with different concentration of O and 2 gradually with advancement in storage. Highest CO extended their improvement in recording high 2 ascorbic acid content than the control. The interaction percent of reducing sugars was documented at 12 effect between the treatments and storage period were days after storage (16.83%). With regard to packaging non-significant. Increase in ascorbic acid content of treatments, the annona fruits packed in PPB flushed custard apple fruit reached a maximum at the with different concentration of O and CO extended 2 2 climacteric, after which the amount decreased. The their improvement in recording lower reducing sugars decline in ascorbic acid in later stage may be than the control. At initial stage of storage, total attributed to conversion of ascorbic acid into sugars was minimal (13.15%), which increased dehydroascorbic acid. Similar result also observed gradually with advancement in storage and total by Paull (1983) in soursop fruits. 7 VENKATRAM et al Table 1. Effect of polypropylene package on days taken for ripening and shelf life (days) of custard apple cv. Balanagar fruits stored at 15±1 oC Days taken Delayed ripening Shelf life Increased shelf life Treatments for ripening over control (%) (days) over control (%) PPB with 3%O +5%CO 9 . 0 0 b 18.42 12.00a 41.17 2 2 PPB with 3%O +10%CO 1 0 . 4 3 a 37.23 11.33c 33.29 2 2 PPB with 5%O +5%CO 1 0 . 4 3 a 37.23 11.33c 33.29 2 2 PPB with 5%O +10%CO 1 0 . 4 3 a 37.23 11.33c 33.29 2 2 PPB with air 8.75b 15.13 12.28a 44.47 Control 7.60c - 8.50d - CD at 5% 0.37 0.58 Figures with same alphabet did not differ significantly (p=0.05) Table 2. Effect of polypropylene package on TSS (oBrix), titrable acidity (%), and brix-acid ratio of custard apple cv. Balanagar fruits stored at 15±1oC Days after storage Mean Treatments 0 2 4 6 8 10 12 TSS (oBrix) PPB with 3%O +5%CO 18.50 18.70 18.75 19.40 20.20 20.30 23.30 19.87c 2 2 PPB with 3%O +10%CO 18.50 18.65 18.65 19.10 19.70 20.30 23.10 19.70c 2 2 PPB with 5%O +5%CO 18.50 18.65 18.65 19.20 19.60 20.35 23.20 19.72c 2 2 PPB with 5%O +10%CO 18.50 18.65 18.65 19.30 19.50 20.40 23.10 19.73c 2 2 PPB with air 18.50 18.85 19.40 20.80 21.70 22.95 24.20 20.90b Control 18.50 19.20 20.20 22.50 23.10 25.00 24.80 22.18a Mean 18.50g 18.78f 19.05e 20.05d 20.63c 21.55b 23.61a Treatments Titrable acidity (%) Mean PPB with 3%O +5%CO 0.16 0.16 0.18 0.24 0.32 0.41 0.46 0.27a 2 2 PPB with 3%O +10%CO 0.16 0.16 0.18 0.24 0.32 0.41 0.46 0.27a 2 2 PPB with 5%O +5%CO 0.16 0.16 0.18 0.24 0.32 0.41 0.46 0.27a 2 2 PPB with 5%O +10%CO 0.16 0.16 0.18 0.24 0.32 0.41 0.46 0.27a 2 2 PPB with air 0.16 0.16 0.18 0.24 0.32 0.41 0.46 0.27a Control 0.16 0.16 0.17 0.20 0.25 0.36 0.36 0.23b Mean 0.16f 0.16f 0.17e 0.23d 0.30c 0.40b 0.44a Treatments Brix-acid ratio Mean PPB with 3%O +5%CO 115.6 116.8 104.1 80.83 63.12 50.00 50.65 82.38c 2 2 PPB with 3%O +10%CO 115.6 116.5 103.6 80.41 60.93 46.75 50.21 82.00c 2 2 PPB with 5%O +5%CO 115.6 116.5 103.6 80.00 61.25 49.63 50.43 82.37c 2 2 PPB with 5%O +10%CO 115.6 116.5 103.6 79.58 61.56 49.51 50.21 82.38c 2 2 PPB with air 115.6 117.8 107.7 86.66 67.81 55.97 52.60 86.31b Control 115.6 120.0 118.8 112.5 92.40 69.44 68.88 99.66a Mean 115.6b 117.3a 106.9c 86.66d 67.84e 53.55f 53.83f CD at 5 % TSS Titrable acidity Brix-acid ratio For days (D) 0.154 0.016 0.95 For treatments (T) 0.220 0.013 0.77 For D × T 0.378 NS 2.32 Figures with same alphabet did not differ significantly (p=0.05); NS- Non significant 8 EFFECT OF POLYPROPYLENE PACKAGING ON SHELF LIFE Table 3. Effect of polypropylene package on reducing sugars (%), non-reducing sugars (%), total sugars (%) and ascorbic acid (mg/100 g) of custard apple cv. Balanagar fruits stored at 15±1oC Days after storage Mean Treatments 0 2 4 6 8 10 12 Reducing sugars PPB with 3%O +5%CO 1 1 . 9 0 11.90 12.10 12.10 13.20 14.40 16.20 13.10c 2 2 PPB with 3%O +10%CO 1 1 . 9 0 1 1.90 12.10 12.10 13.20 14.30 16.10 13.08c 2 2 PPB with 5%O2+5%CO2 1 1 . 9 0 11.90 12.10 12.10 13.20 14.40 16.20 13.10c PPB with 5%O +10%CO 1 1 . 9 0 1 1.90 12.10 12.10 13.20 14.40 16.20 13.10c 2 2 PPB with air 11.90 11.90 12.30 12.50 14.00 15.80 17.20 13.64b Control 11.90 12.00 12.90 13.50 15.50 18.10 19.10 15.48a Mean 11.90f 11.91f 12.26e 12.40d 13.71c 15.23b 16.83a Treatments Non-reducing sugars Mean PPB with 3%O +5%CO 1 . 2 5 1.25 1.25 1.30 1.41 1.41 1.57 1.34c 2 2 PPB with 3%O +10%CO 1 . 2 5 1.25 1.25 1.30 1.41 1.41 1.57 1.34c 2 2 PPB with 5%O +5%CO 1 . 2 5 1.25 1.25 1.30 1.41 1.41 1.57 1.34c 2 2 PPB with 5%O +10%CO 1 . 2 5 1.25 1.25 1.30 1.41 1.41 1.57 1.34c 2 2 PPB with air 1.25 1.25 1.35 1.43 1.48 1.53 1.62 1.40b Control 1.25 1.25 1.47 1.55 1.65 1.75 1.71 1.51a Mean 1.25e 1.25e 1.30d 1.36c 1.46b 1.48b 1.60a Treatments Total sugars Mean PPB with 3%O +5%CO 1 3 . 1 5 13.15 13.35 13.40 14.61 15.81 17.77 14.40c 2 2 PPB with 3%O +10%CO 1 3 . 1 5 1 3.15 13.35 13.40 14.61 15.71 17.67 14.39c 2 2 PPB with 5%O +5%CO 1 3 . 1 5 13.15 13.35 13.40 14.61 15.81 17.77 14.40c 2 2 PPB with 5%O +10%CO 1 3 . 1 5 1 3.15 13.35 13.40 14.61 15.81 17.77 14.40c 2 2 PPB with air 13.15 13.15 13.65 13.93 15.48 17.33 18.82 15.06b Control 13.15 13.25 14.37 15.05 17.15 19.85 20.81 16.22a Mean 13.15f 13.16f 13.57e 13.76d 15.17c 16.72b 18.43a Treatments Ascorbic acid Mean PPB with 3%O +5%CO 4 1 . 3 0 41.30 41.30 50.20 45.10 43.60 32.50 42.17a 2 2 PPB with 3%O +10%CO 4 1 . 3 0 4 1.30 41.30 50.20 45.10 43.60 32.50 42.17a 2 2 PPB with 5%O +5%CO 4 1 . 3 0 41.30 41.30 50.20 45.10 43.60 32.50 42.17a 2 2 PPB with 5%O +10%CO 4 1 . 3 0 4 1.30 41.30 50.16 45.10 43.60 32.50 42.17a 2 2 PPB with air 41.30 41.30 41.30 48.40 44.40 40.75 30.22 41.07a Control 41.30 41.30 41.5 47.20 42.25 38.55 28.66 40.10b Mean 41.30c 41.30c 41.33c 49.39a 44.50b 42.28c 31.48d CD at 5 % Reducing sugars Non-reducing sugars Total sugars Ascorbic acid For days (D) 0.118 0.042 0.071 1.650 For treatments (T) 0.096 0.034 0.058 1.350 For D T 0.289 0.104 0.175 NS Figures with same alphabet did not differ significantly (p=0.05); NS- Non significant 9 VENKATRAM et al REFERENCES Alique, R. 1995. 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