Conférence nationale GdR Phycotox & GIS Cyano 2017

95 Pages·2017·1.86 MB·English

Conférence nationale
GdR Phycotox & GIS Cyano 2017
Résumés des présentations
et des posters

14 - 16 Mars 2017,
Gif-sur-Yvette (France)
GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
Comité scientifique d’organisation

Romulo ARAOZ ......................................... CNRS – Gif-sur-Yvette
Hélène HEGARET ...................................... IUEM – LEMAR - Brest
Philipp HESS ............................................. IFREMER – Nantes DYNECO/PHYC
Christophe LAPLACE-TREYTURE ........... IRSTEA - Bordeaux
Delphine LATOUR ..................................... UNIVERSITE de Clermont-Ferrand
Benjamin MARIE ....................................... MNHN – Paris
Joël ROBIN ................................................ ISARA – Lyon
Raffaele SIANO .......................................... IFREMER – Brest DYNECO/Pelagos
Anne THEBAULT ....................................... ANSES – Maisons-Alfort.

Remerciements:
Nous souhaitons remercier le CNRS de Gif-sur-Yvette d’avoir accueilli cette conférence dans ses
locaux. Que soit aussi remercié le CNRS, Irstea, l’Ifremer, l’ANSES et les sociétés Novakits et
Bionef pour leur soutien financier.
Enfin, nous remercions toutes les personnes qui se sont impliquées de près ou de loin dans
l’organisation de cette conférence et toutes celles qui apporteront leur aide précieuse pour en
assurer le bon déroulement le jour J.

Couverture : schéma réalisé par Romulo Araoz (CNRS – Gif/Yvette),
mise en page par Françoise Peyriguer (Irstea – Antony).
GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
Program

Tuesday 14 March 2017

13:30-14:00 Welcome coffee and inscription
14:00-14:15 Introductory speech
14:15-15:00 Invited conference: The Integrated Pest Management Model as a Framework
for Aquaculture HAB Mitigation
Gary Wikfors

15:00-16:00 THEME: Effects of HABs on professional sectors and society at large: from
legal and socio-economic perspectives
Chairmans: Christophe Laplace-Treyture & Rodolphe Lemée

15:00-15:15 Coordinating the implementation of the interagency Global CFP Strategy
Mireille Chinain, Marie-Yasmine Dechraoui Bottein, Philipp Hess
15:15-15:30 Pinnatoxins, an emergent class of fast-acting marine toxins, are potential threat for
neurodevelopment and global health
Sophie Creuzet, Aurélie Couesnon, Vincent Dive, Armen Zakarian, Jordi Molgó, Denis
Servant
15:30-15:45 Eaters of oyster: From the risk of contamination by toxic algae to social
representations and practices of consumption
Elisabeth Guillou, Adeline Raymond
15:45-16:00 Thinking out of the box: finding new ways of regulating marine toxins
Marie Bonnin, Hélène Hégaret, Philippe Soudant, Philippe Fotso, Caroline Fabioux, Philipp
Hess

16:00-16:30 Coffee break

16:30-18:15 THEME: Tropics
Chairmans: Christophe Laplace-Treyture & Rodolphe Lemée

16:30-16:45 Ciguatera incidence in the Caribbean Islands
Jose Ernesto Mancera
16:45-17:00 Microalgae from French Polynesian lagoons: novel source of bioactive
compounds?
Mireille Chinain, Sebastien Longo, Kevin Henry, André Ung, Taiana Darius, Jérôme Viallon,
Martine Rodier, Bruno Delesalle, Alain Lo-Yat, Gilles Le Moullac, Romulo Araoz
17:00-17:15 Distribution and abundances of benthic toxic dinoflagellates in French West Indies
Aurélie Boisnoir, Pierre-Yves Pascal, Sophie Marro, Rodolphe Lemée
17:15-17:30 Taxonomic diversity of the dinoflagellate genus Ostreopsis in French Polynesia:
preliminary results from Marquesas Islands (Pacific Ocean)
Nicolas Chomérat, Clémence Gatti, Gwenael Bilien, André Ung, H. Taiana Darius, R.
Wayne Litaker, Patricia Tester, Mireille Chinain
17:30-17:45 Taxonomic characterization of Gambierdiscus species in Reunion Island, Indian
Ocean
Alina Tunin-Ley, Nicolas Chomérat, Gwenael Bilien, Jean Turquet

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GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
17:45 18:00 Environmental control and dynamics of blooms of the benthic toxic dinoflagellate
Ostreopsis cf. ovata in the Mediterranean Sea
Cécile Jauzein, Rodolphe Lemée
18h00 18h15 Ostreopsis blooms at the global scale: influence of macroalgal communities
Luisa Passeron Mangialajo, Rodolphe Lemée, Anna Fricke, Daniela Catania, Sarah Kulins,
Cécile Jauzein, Nick Shears

18:15-19:00 GdR Phycotox Annual general meeting

19:00-20:00 Welcome cocktail

Wednesday 15 March 2017

09:00-09:45 Invited conference: The diatom genus Pseudo-nitzschia in the Gulf of Naples
(Mediterranean Sea): exploring diversity and life history traits
Marina Montresor

09:45-10:45 THEME: Ecology of HAB
Chairmans: Delphine Latour & Mathilde Schapira

09:45-10:00 Genetic, morphological and ecological evidence of new oomycete parasites of the
toxic diatom genus Pseudo-nitzschia
Garvetto Andrea, Claire Gachon, Elisabeth Nézan, Raffaele Siano
10:00-10:15 Effects of salinity variation on the physiology and domoic acid production by two
strains of Pseudo-nitzschia australis
Nour Ayache, Amandine Caruana, Fabienne Hervé, Zouher Amzil
10:15-10:30 Interspecific and intraspecific variability in physiology among four Pseudo-nitzschia
species
Aurore Sauvey, Pascal Claquin, Juliette Fauchot
10:30-10:45 Nutrient ratios influence variability in Pseudo-nitzschia species diversity and
particulate domoic acid production in the Bay of Seine (France)
Juliette Fauchot, Maxine Thorel, Mathilde Schapira, Romain Le Gendre, Philippe Riou,
Didier Goux, Bertrand Le Roy, Virginie Raimbault, Anne-Flore Deton-Cabanillas, Pauline
Bazin, Valérie Kientz-Bouchart, Pascal Claquin

10:45-11:15 Coffee break

11:15-12:30 THEME: Diversity and theoretical ecological niches of HAB, synthesis and
transformation of metabolites, analytical challenges
Chairmans: Delphine Latour & Mathilde Schapira

11:15-11:30 Benthic Planktothrix and their antifungal natural products
Claire Pancrace, Jouni Jokela, Nathalie Sassoon, Christelle Ganneau, Marie Desnos-
Ollivier, Matti Wahlsten, Anu Humisto, Alexandra Calteau, Sylvie Bay, David P. Fewer,
Kaarina Sivonen, Muriel Gugger
11:30-11:45 From cyanobacteria to mammals: role of ergothionein, glutathione and its analogs
in stressors signalling and detoxication
Kinsley Narainsamy, Christophe Junot, Corinne Cassier-Chauvat, Franck Chauvat
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GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
11:45-12:00 Latest advances in two functional methods to detect ciguatoxins (CTXs): the
Receptor Binding Assay (RBA) & the Cell Based Assay (CBA-N2a)
Taiana Darius, Jérôme Viallon, Jennifer Mc Call, Taina Revel, Ransom Hardison, William
Holland, Patricia Tester, Wayne Litaker, Mireille Chinain
12:15-12:30 Rapid characterization of microcystin-producing cyanobacteria in freshwater lakes
by TSA-FISH (Tyramid Signal Amplification - Fluorescent In Situ Hybridization)
Isabelle Biegala, Luc Brient

12:30-14:00 Lunch

14:00-:14:45 Invited conference: The Užice Case
Jussy Meriluoto, Zorica Svirčev

14:45-16:15 THEME: Human and animal toxicology
Chairmans: Benjamin Marie & Ronel Biré

14:45-15:00 Mixtures of lipophilic phycotoxins: exposure data and in vitro toxicity assessment
Jimmy Alarcan, Ronel Biré, Ludovic Le Hegarat, Valérie Fessard
15:00-15:15 Ostreopsis cf. ovata blooms in the NW Mediterranean: Exploring the links between
toxins variability and impacts on human health
Elisa Berdalet, Rafael Abós-Herràndiz, Marta Estrada
15:15-15:30 Maitotoxin-4, a novel MTX analog produced by Gambierdiscus excentricus
Francesco Pisapia, Manoëlla Sibat, Christine Herrenknecht, Korian Lhaute, Greta Gaiani,
Pierre-Jean Ferron, Valérie Fessard, Catherine Roullier, Santiago Fraga, Silvia
Nascimento, Wayne Litaker, Philipp Hess
15:30-15:45 Transgenerational effects of Microcystin-LR on Daphnia magna
Claudia Wiegand, Rafael Ortiz-Rodríguez, Thanh Dao
15:45-16:00 Gambierol enhances quantal acetylcholine release from motor nerve terminals of
vertebrate neuromuscular junctions
Jordi Molgó, Sébastien Schlumberger, Makoto Sasaki, Haruhiko Fuwa, M. Louzao, Luis
Botana, Evelyne Benoit
16:00-16:15 Receptor-based lateral flow test for fast and early detection of neurotoxins acting
on nicotinic acetylcholine receptors
Romulo Araoz, Denis Servent

16:15-16:45 Coffee break

16:45-17:15 POSTER SESSION: My poster in 3 minutes
Chairman: Valérie Fessard

17:15-19:30 POSTER SESSION

18:30-19 :30 Réunion du comité de pilotage pour préparer la conférence ICHA 2018 à Nantes

19:30 Diner cocktail

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GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette

Thursday 16 March 2017

09:00-09:45 Invited conference: Differential Effects of Algal Toxins on Grazing by the
Marine Copepod Acartia tonsa
Patricia Tester, Jiarong Hong, Rebecca Waggett, Joseph Katz, Allen Place,
Erik Davenport

09:45-10:45 THEME: Effects of HAB on marine organisms and ecosystems, trophic
transfer, etc.
Chairmans: Juliette Fauchot & Cécile Bernard

09:45-10:00 Interest of bivalves for the biosurvey of environmental contamination by
cyanobacterial toxins
Emilie Lance, Zouher Amzil, Alexandra Lepoutre, Luc Brient, Myriam Bormans
10:00-10:15 Impacts of Alexandrium minutum on the reproduction of the oyster Crassostrea
gigas: from gametogenesis to larval settlement
Justine Castrec, Philippe Soudant, Marianne Alunno Bruscia, Bruno Petton, Isabelle Queau,
Marc Suquet, Dominique Ratiskol, Jacqueline Le Grand, Christian Mingant, Nelly Le Goic,
Hélène Hégaret, Caroline Fabioux
10:15-10:30 Impact of the environmental toxin BMAA on the immune system of the zebra mussel
Dreissena polymorpha.
Alexandra Lepoutre, Nadia Milliote, Mélissa Palos Ladeiro, Marc Bonnard, Elisabeth
Faassen, Alain Geffard, Emilie Lance
10:30-10:45 Metabolomics for cyanobacteria ecotoxicology: insights from the Toxcyfish project
Benjamin Marie, Benoit Sotton, Séverine Le Manach, Alain Paris

10:45-11:15 Coffee break

11:15-11:30 Short term effects of the allelochemicals released by the toxic dinoflagellate
Alexandrium minutum upon the diatom Chaetoceros sp.
Marc Long, Kevin Tallec, Philippe Soudant, Fabienne Le Grand, Géraldine Sarthou, Dianne
Jolley, Hélène Hégaret
11:30-11:45 Phenolic compounds from the seagrasses Zostera marina and Zostera noltei affect
negatively the physiology of the neurotoxic dinoflagellate Alexandrium catenella
Mohamed Laabir
11:45-12:00 Paralytic Shellfish Toxin accumulation in oyster is predicted by gene expression
Audrey Mat, Christophe Klopp, Laura Payton, Céline Jeziorski, Morgane Chalopin, Zouher
Amzil, Damien Tran, Hélène Hégaret, Philippe Soudant, Caroline Fabioux, Arnaud Huvet
12:00-12:15 The Paralytic Shellfish Toxin, Saxitoxin, enters the cytoplasm and induces
apoptosis of oyster immune cells via a caspases-dependent pathway
Jean-Luc Rolland, Célina Abi-Khalil, Darren Finkelstein, Genevieve Conejero, Justin Du
Bois, Delphine Destoumieux-Garzon
12:15-12:30 Continued feeding with Gambierdiscus polynesiensis causes increasing
accumulation of ciguatoxin over 16 weeks in an herbivorous reef fish
Rachel Clausing, Barbara Losen, Taiana Darius, Philipp Hess, Manoella Sibat, Jerome
Viallon, Mireille Chinain, Marie-Yasmine Dechraoui Bottein
12:30-12:45 Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations
with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea
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GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
gigas
Floriane Boullot, Justine Castrec, Adeline Bidault-Toffin, Natanael Dantas, Laura Payton,
Mickael Perrigault, Damien Tran, Zouher Amzil, Pierre BOUDRY, Philippe Soudant,
Hélène Hegaret, Caroline Fabioux

12:45-14:15 Lunch

14:15-15:00 Invited conference: Strategies to reduce toxins in shellfish
Ana Garcia Cabado

15:00-16:15 THEME: Diversity and theoretical ecological niches of HAB, synthesis and
transformation of metabolites, analytical challenges
Chairmans: Joël Robin & Elisa Berdalet

15:00-15:15 Monitoring and modelling cyanobacteria biomass in a small urban lake
Denis Plec, Lucas Jardim Porto, Bruno Lemaire, Philippe Dubois, Mohamed Saad, Cécile
Bernard, Laure Huguenard, Brigitte Vinçon-Leite
15:15-15:30 Temperature and stratification of the artificial lake and the proliferation of
cyanobacteria, case of the lake of the Eguzon dam - France
Zoubida Qsair, Laurent Touchart, Pascal Bartout
15:30-15:45 Genetic diversity of the toxic bloom-forming cyanobacterium Microcystis
aeruginosa: a meta-analysis at large spatial and temporal scales
Marion Sabart, Benjamin Misson, Marlène Jobard, Gaofei Song, Li Renhui, Delphine Latour
15:45-16:00 Wise use of metabolomics in the field of marine chemical ecology
Eva Ternon, Anne-Sophie Pavaux, Olivier Thomas
16:00-16:15 Metabolomics of ancient populations of DINoflAgellates in phosphorous-limited
conditions
Raffaele Siano, Florence Mondeguer, Marie Latimier, Julien Quéré, Manoella Sibat, Laure
Guillou, Philipp Hess

16:15 End of the symposium

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GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
Table of contents

Theme 1: Diversity and theoretical ecological niches of HAB, synthesis and
transformation of metabolites, analytical challenges ..........................................................12

Rapid characterization of microcystin-producing cyanobacteria in freshwater lakes by TSA-
FISH (Tyramid Signal Amplification - Fluorescent In Situ Hybridization),
Isabelle Biegala [et al.] ......................................................................................................13

Latest advances in two functional methods to detect ciguatoxins (CTXs): the Receptor
Binding Assay (RBA) & the Cell Based Assay (CBA-N2a),
Taiana Darius [et al.] ........................................................................................................14

From cyanobacteria to mammals: role of ergothionein, glutathione and its analogs in stressors
signalling and detoxication,
Kinsley Narainsamy [et al.]. ..............................................................................................15

Benthic Planktothrix and their antifungal natural products,
Claire Pancrace [et al.] ......................................................................................................16

Monitoring and modelling cyanobacteria biomass in a small urban lake,
Denis Plec [et al.]. ............................................................................................................17

Temperature and stratification of the artificial lake and the proliferation of cyanobacteria, case
of the lake of the Eguzon dam - France,
Zoubida Qsair [et al.] ........................................................................................................18

Genetic diversity of the toxic bloom-forming cyanobacterium Microcystis aeruginosa: a
meta-analysis at large spatial and temporal scales,
Marion Sabart [et al.]. ......................................................................................................19

MEtabolomics of ancient populations of DINoflAgellates in phosphorous-limited conditions,
Raffaele Siano [et al.] ........................................................................................................20

Wise use of metabolomics in the field of marine chemical ecology,
Eva Ternon [et al.]. ...........................................................................................................21

Theme 2: Ecology of HAB ....................................................................................................22

Genetic, morphological and ecological evidence of new oomycete parasites of the toxic
diatom genus Pseudo-nitzschia,
Garvetto Andrea [et al.] ....................................................................................................23

Effects of salinity variation on the physiology and domoic acid production by two strains of
Pseudo-nitzschia australis,
Nour Ayache [et al.] ..........................................................................................................24

Nutrient ratios influence variability in Pseudo-nitzschia species diversity and particulate
domoic acid production in the Bay of Seine (France),
Juliette Fauchot [et al.] .....................................................................................................25

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GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
The diatom genus Pseudo-nitzschia in the Gulf of Naples (Mediterranean Sea): exploring diversity
and life history traits,
Marina Montresor ........................................................................................................................ 26

Interspecific and intraspecific variability in physiology among four Pseudo-nitzschia species,
Aurore Sauvey [et al.] ........................................................................... 27

Theme 3: Effects of HAB on marine organisms and ecosystems, trophic transfer, etc. 28

Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with
Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas,
Floriane Boullot [et al.] .....................................................................................................29

Impacts of Alexandrium minutum on the reproduction of the oyster Crassostrea gigas:
from gametogenesis to larval settlement,
Justine Castrec [et al.] ......................................................................................................30

Continued feeding with Gambierdiscus polynesiensis causes increasing accumulation of
ciguatoxin over 16 weeks in an herbivorous reef fish,
Rachel Clausing [et al.]. ....................................................................................................31

Phenolic compounds from the seagrasses Zostera marina and Zostera noltei affect negatively
the physiology of the neurotoxic dinoflagellate Alexandrium catenella,
Mohamed Laabir ...............................................................................................................32

Interest of bivalves for the biosurvey of environmental contamination by cyanobacterial toxins,
Emilie Lance [et al.] ..........................................................................................................33

Impact of the environmental toxin BMAA on the immune system of the zebra mussel
Dreissena polymorpha,
Alexandra Lepoutre [et al.] ...............................................................................................34

Short term effects of the allelochemicals released by the toxic dinoflagellate Alexandrium
minutum upon the diatom Chaetoceros sp..
Marc Long [et al.] .............................................................................................................35

Metabolomics for cyanobacteria ecotoxicology: insights from the Toxcyfish project,
Benjamin Marie [et al.] .....................................................................................................36

Paralytic Shellfish Toxin accumulation in oyster is predicted by gene expression,
Audrey Mat [et al.]. ..........................................................................................................37

The Paralytic Shellfish Toxin, Saxitoxin, enters the cytoplasm and induces apoptosis of
oyster immune cells via a caspases-dependent pathway,
Jean-Luc Rolland [et al.] ..................................................................................................38

Differential Effects of Algal Toxins on Grazing by the Marine Copepod Acartia tonsa,
Patricia Tester [et al.] .......................................................................................................39

7
GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette
Theme 4: Human and animal toxicology ..............................................................................40

Receptor-based lateral flow test for fast and early detection of neurotoxins acting on
nicotinic acetylcholine receptors.,
Romulo Araoz [et al.]........................................................................................................41

Mixtures of lipophilic phycotoxins: exposure data and in vitro toxicity assessment,
Jimmy Alarcan [et al.] ......................................................................................................42

Ostreopsis cf. ovata blooms in the NW Mediterranean: Exploring the links between toxins
variability and impacts on human health,
Elisa Berdalet [et al.] ........................................................................................................43

The Užice Case,
Jussi Meriluoto [et al.] .......................................................................................................................... 44

Gambierol enhances quantal acetylcholine release from motor nerve terminals of vertebrate
neuromuscular junctions,
Jordi Molg´o [et al.] ...........................................................................................................45

Maitotoxin-4, a novel MTX analog produced by Gambierdiscus excentricus,
Francesco Pisapia [et al.]. ................................................................................................46

Transgenerational effects of Microcystin-LR on Daphnia magna,
Claudia Wiegand [et al.]. ..................................................................................................47

Theme 5: Effects of HABs on professional sectors and society at large: from legal and
socio-economic perspectives ................................................................................................ 48

Thinking out of the box: finding news ways of regulating marine toxins,
Marie Bonnin [et al.] .........................................................................................................49

Coordinating the implementation of the interagency Global CFP Strategy,
Mireille Chinain [et al.]. ....................................................................................................50

Pinnatoxins, an emergent class of fast-acting marine toxins, are potential threat for
neurodevelopment and global health,
Sophie Creuzet [et al.]. ......................................................................................................51

Strategies to reduce toxins in shellfish,
Ana Garcia-Cabado .........................................................................................................52

Eaters of oyster: From the risk of contamination by toxic algae to social representations
and practices of consumption,
Elisabeth Guillou [et al.] ...................................................................................................53

The Integrated Pest Management Model as a Framework for Aquaculture HAB Mitigation,
Gary Wikfors ....................................................................................................................54

8