Complete thesis

213 Pages·2017·14.61 MB·English

by  Magdalena Golachowska

University of Groningen

Microvillus Inclusion Disease. Lessons about the apical plasma membrane.
Golachowska, Magdalena Renata
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Golachowska, M. R. (2011). Microvillus Inclusion Disease. Lessons about the apical plasma membrane.
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Download date: 08-01-2023
Microvillus Inclusion Disease
Lessons about the apical plasma membrane

Magdalena R. Golachowska
This research was financially supported by the University of Groningen
University Medical Center Groningen

The research described in this dissertation was conducted at the Department of
Cell Biology, section Membrane Cell Biology, University Medical Center
Groningen, University of Groningen, Groningen, The Netherlands

This thesis is available at the Library of University of Groningen.

Printing of this thesis was financially supported by:

RijksUniversiteit Groningen, University Medical Center Groningen
Graduate School for Drug Exploration (GUIDE)
Nederlandse Vereniging voor Gastro-Enterologie (NVGE)
Sectie Experimentele Gastroenterologie (SEG) of the NVGE

Printed by: GS Media, Wroclaw, Poland

© 2011 by M.R.Golachowska
All rights reserved. No part of this book may be reproduced or transmitted in any
form or by any means without written permission of the author and the publisher
holding the copyright of the published articles.

Cover: Electron micrograph of microvillus inclusion from a duodenal enterocyte
of a MVID-diagnosed patient processed with graphic software.

ISBN: 978-83-61039-78-5
(Wydawnictwo EUROPA)
RIJKSUNIVERSITEIT GRONINGEN

Microvillus Inclusion Disease
Lessons about the apical plasma membrane

Proefschrift

ter verkrijging van het doctoraat in de
Medische Wetenschappen
aan de Rijksuniversiteit Groningen
op gezag van de
Rector Magnificus, dr. E. Sterken,
in het openbaar te verdedigen op
maandag 11 juli 2011
om 16.15 uur

door

Magdalena Renata Golachowska

geboren op 27 december 1981
te Wrocław, Polen
Promotor: Prof. dr. D. Hoekstra

Copromotor: Dr. S.C.D. van IJzendoorn

Beoordelingscommissie: Prof. dr. K.N. Faber
Prof. dr. R.M.W. Hofstra
Prof. dr. H.J. Verkade
Paranimfen: Herschel Dhekne
Péter Mészáros

Rodzicom

CONTENTS

Chapter 1 The epithelial apical plasma membrane: structure- 13
function relationships and dynamics at the molecular
level

Chapter 2 Functional characterization of mutations in the myosin Vb 37
gene associated with microvillus inclusion disease

Chapter 3 Divergent effects of MYO5B mutations on apical brush 65
border and apical recycling endosome organization in
kidney and intestinal epithelial cells of Microvillus
Inclusion Disease patients presenting with renal Fanconi
syndrome

Chapter 4 Extracellular acidosis induces Rho Kinase/myosin-II- 85
mediated vacuolarization of the apical plasma membrane
and formation of intracytoplasmic apical vacuoles

Chapter 5 The complex organization and apical positioning of the 109
Golgi complex is dispensable for polarized apical
trafficking and polarity in hepatoma HepG2 cells at steady
state

Chapter 6 Recycling endosomes in apical plasma membrane domain 131
formation and epithelial cell polarity

Chapter 7 Summary, Discussion and Perspectives 151

Nederlandse samenvatting 157
Streszczenie w języku polskim 161
References 167
Appendix 1 (Supplementary Figures for Chapter 2) 195
Appendix 2 (Supplementary Figures for Chapter 4) 205
Acknowledgements 209