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Complete Chromosome Sequence of Carnobacterium maltaromaticum LMA 28 CatherineCailliez-Grimal,aStéphaneChaillou,b,cJamilaAnba-Mondoloni,b,cValentinLoux,dMuhammadInamAfzal,aAbdurRahman,a GillesKergourlay,e,fMarie-ChristineChampomier-Vergès,b,cMoniqueZagorec,b,c,e,fPawDalgaard,gJorgenJ.Leisner,h HervéPrévost,e,fAnne-MarieRevol-Junelles,aFrédéricBorgesa UniversitédeLorraine,Laboratoired’IngénieriedesBiomolécules(LIBio),Vandoeuvre-lès-Nancy,Francea;INRA,UMR1319Micalis,Jouy-en-Josas,Franceb;AgroParisTech, UMRMicalis,Jouy-en-Josas,Francec;UnitédeMathématique,InformatiqueetGénome,INRA,Jouy-en-Josas,Franced;INRA,Nantes,Francee;L’UniversitéNantesAngersLe Mans(L’UNAM),Oniris,UMR1014Secalim,Nantes,Francef;NationalFoodInstitute(DTUFood),TechnicalUniversityofDenmark,Kgs.Lyngby,Denmarkg;Departmentof VeterinaryDiseaseBiology,FacultyofHealthandMedicalSciences,UniversityofCopenhagen,Copenhagen,Denmarkh WithinthelacticacidbacteriumgenusCarnobacterium,Carnobacteriummaltaromaticumisoneofthemostfrequentlyisolated speciesfromnaturalenvironmentsandfood.Itpotentiallyplaysamajorroleinfoodproductbiopreservation.Wereporthere onthe3.649-MbchromosomesequenceofC.maltaromaticumLMA28,whichwasisolatedfromripenedsoftcheese. Received8November2012 Accepted19November2012 Published31January2013 CitationCailliez-GrimalC,ChaillouS,Anba-MondoloniJ,LouxV,AfzalMI,RahmanA,KergourlayG,Champomier-VergèsMC,ZagorecM,DalgaardP,LeisnerJJ,PrévostH, Revol-JunellesAM,BorgesF.2013.CompletechromosomesequenceofCarnobacteriummaltaromaticumLMA28.GenomeAnnounc.1(1):e00115-12.doi:10.1128/ genomeA.00115-12. Copyright©2013Cailliez-Grimaletal.Thisisanopen-accessarticledistributedunderthetermsoftheAttribution3.0UnportedCreativeCommonsLicense. AddresscorrespondencetoCatherineCailliez-Grimal,[email protected]. ThegenusCarnobacteriumbelongstothelacticacidbacteria, taromaticummightexplaintheabilityofthisspeciestoadaptto andcurrentlyconsistsof11species.Carnobacteriummaltaro- multipleanddiverseenvironmentscomparedtotheothercarno- maticumstrainsarewidelyfoundinfoods,includingdairyprod- bacterialspecies.Thisgenomictraitisillustratedbythepresence ucts(1).Thisspecieshaspotentialforapplicationasaprotective of genes involved in the metabolism of branched-chain amino cultureinfoods.Mostresearchhasfocusedontheproductionof acids. bacteriocins,ontheirrolesintheinhibitionofListeriamonocyto- Indeed, the species C. maltaromaticum is well known for its genes, and on the regulation of metabolic pathways of sensory abilitytoproducetheflavorcompound3-methylbutanal,whichis importance(2).C.maltaromaticumLMA28wasisolatedfroma theresultofleucinecatabolism.Inlacticacidbacteria,themore softripenedcheese(3). prevalent pathway is the (cid:1)–keto acid dehydrogenase (KaDH) ThegenomesequenceofC.maltaromaticumLMA28wasde- pathway (8). A less common alternative pathway is the (cid:1)–keto terminedusing454pyrosequencingGS-FLXsystem(Roche454 aciddecarboxylasepathway,encodedbygenekdcA.Thisgeneis Life Sciences, Mannheim, Germany) and Illumina sequencing. onlydescribedfortwostrainsofL.lactis(8).Inthegenomese- Pyrosequencingruns,includingshotgunandpaired-endruns,re- quencesofC.maltaromaticumLMA28andATCC35586,anor- sultedin15scaffoldscontaining123contigsand55-foldcoverage. thologous gene of kdcA was found (2) that is absent from the AsubsequentIlluminasequencingrunperformedwithapaired- genomesoftheotherCarnobacteriumstrains. endlibrarycorrected923indels.PCR-basetechniquesandSanger Nucleotidesequenceaccessionnumber.Thecompletechro- sequencingoftheproductswereusedtoclosetheremaininggaps. mosome sequence of Carnobacterium maltaromaticum LMA 28 ThemanuallycuratedsequenceofLMA28comprisesonechro- hasbeendepositedatEMBL/GenBankundertheaccessionnum- mosomeof3,649,737bpwithanoverallG(cid:1)Ccontentof34.5%. berno.HE999757. Coding sequence (CDS) predictions and annotations were per- formed with Integrative Services for Genomics Analysis (ISGA) ACKNOWLEDGMENTS (4)andprovided3,933predictedCDSs,59tRNAgenes,6rRNA WethankMyriamMichelandArnaudKhemistiforprovidingexcellent operons,andasingle5SrRNAgene. technicalassistance. Sofar,threegenomicsequencesofCarnobacteriumhavebeen ThisworkwassupportedpartlybytheFabelorCompetenceCluster, published:thecompletegenomesequenceofCarnobacteriumsp. Nancy,France. 17-4(5)isolatedfrompermanentcoldseawater;thedraftgenome sequences of C. maltaromaticum ATCC 35586, isolated from a REFERENCES diseasedsalmon(6);andthedraftgenomeofCarnobacteriumsp. 1. AfzalMI,JacquetT,DelaunayS,BorgesF,MillièreJB,Revol-Junelles AT7, a piezophilic strain isolated from the Aleutian trench (7). AM,Cailliez-GrimalC.2010.Carnobacteriummaltaromaticum:identifi- ThegenomesizeofthestrainATCC35586(3.5Mbp)issimilarto cation,isolationtools,ecologyandtechnologicalaspectsindairyproducts. 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